Click here to switch to the map view.

The map label for this gene is topA

Identifier: 21672553

GI number: 21672553

Start: 309636

End: 312197

Strand: Direct

Name: topA

Synonym: BUsg273

Alternate gene names: 21672553

Gene position: 309636-312197 (Clockwise)

Preceding gene: 21672552

Following gene: 21672555

Centisome position: 48.27

GC content: 25.57

Gene sequence:

>2562_bases
ATGTGTAAATCTTTAGTTATTGTTGAATCACCAGTAAAAGCAAAAACTATAAGTAAATATTTAGGTAAGCAATATATAGT
CAAATCTAGTGTGGGTCATGTACGAGATTTAATAAATAATAAATCTAAAAACAAAAAAAATATTAAAAAATCTAATATCA
AACATAATGAAAAAACAGCTTTAATACAACAAATGGGGATTAATCCATATAAAAATTGGAAAGCAGAATATCATATTTTA
CCTGGTAAGGAAAAAATTATTTCTGAGTTAAAAAATATTGCAAATAAAGTTCACTATATATATCTTGCTACTGATTTAGA
TAGAGAAGGTGAAGCAATAGCGTGGCATTTAAAAGAGGTAATTGGAGGAAATTCTCTTAAATTTAGACGTGTTGTTTTTA
ATGAAATAACTAAAAAATCAATTGAAAAAGCTTTTCAAAATATTGGTAAAATTAATATGAACCGGGTGTATTCGCAACAA
GCTCGTCGATTTATGGATAGAATTGTAGGCTATATGATTTCACCTTTATTATGGAAAAAAATCTCAAGAGGATTATCTGC
AGGTCGTGTTCAATCTGCAGCTGTTCGACTTATAACAGATCGAGAATATGAAATAAAAAATTTTATCGAACGTGAACATT
GGAAAATTAGTCTTTCTCTTTTATCTAAAGAAAATAAAAAAGTCACGATGGATATAACACATTATAGAAATAAAACATTT
TTATTAAATAATAAAAAAGAGGTAAATTCTACAATAGAAAAAATAAAAAATTTATCTTTTATTATTACAGATCGAAAGGA
TAAAATTTTCAAAAAAAAACCACCAGCTCCATTAATTACCTCGACGTTACAACAAGCTTCTAATATTGATTTGGGATTTA
GTGTAAAAAAAACAATGTTTTTAGCGCAAAAATTATATGAGCAAGGATATATAACTTATATAAGAACTGATTCTTATTTT
TTAAGTAATTATGCTATTGAAAAAGTTAGATCGTATATAGAAAATTTTTATGGAAGTGATTTTTTGCCTAAAAAATCAAA
TATATACTCAAATCAAAAATATTCTCAAGAAGCTCATGAAGCTATTCGACCTTCAGATGTCCAAGTTGTAAATATTGATA
ATTGTGATCCAGATGCTAAAAAATTATATAAATTAATTTGGAATTATTTTATTGCTTCTCAAATGAAATCAGAAAGATAT
AAATCTATTAAAACGACAGTTATGGCTGATGTATTTAAATTACAGTCAAATACAAAAATAGTGCTCTTTTCAGGATGGAC
TAAAATTTTGAAAAAATCAAATAATGTTAATTTTAAATTTCCTGTTTTAGATATAGGAACTACATTGATTTTAGATAAAA
TTTTGCCTCATCAAATCTTTACTAAGCCTCCACCACGTTTTAGTGAGTCATCTTTAGTACGTGAATTAGAAAAAAAAGGT
ATTGGAAGACCTTCTACCTACGCTACTATAATAACGAAAATAAAAGAAAAAGGGTATTTAAAGATAAAAAAAAATAAATT
TTATGCTGCAAAAATAGGAGAAATTCTTATAACTCGATTGAAAAAAAATTTTTCTGATTTAGTTGATTATGATTTTACAG
CTCGTATGGAAAAAAATCTTGATCAAGTTTCTGATAAATTAATTAATTGGAAACATTTGTTGAATTCTTTTTTTGATAAT
TTTTCTCAACAGTTAGAACAAGCAAAAAAACCTCCTGAAGAAGGTGGTATGGAACTTAATACGACTGTTCCGACTGAAAT
TGATTGCTCTTTATGTAATAAAAAAATGGGGATTAAAACCGCTGTTACAGGTGTATTTTTAAGCTGTTTAGGATATAATT
CAGAACCTAATGAAAAACGCTGTAAAAATACAATAAATTTAATTTCATTAAATGATTTGAGTACAACAAAAAAAGAAGAA
AAAAATTTTCATTTAAAAAATAGATGTAAAAAATGCAATTTGGTAATGGACGTGTATTTAATTAATGAAAATTTAAAAAT
TTTCATTTGTATAAATAATCCTAGTTGTAATGGTTACAATCTTAAAAAAGGCATATTTAAAAAAAGTTTAAATTGCTCAT
TAAAAAAAATAAAATGTGAAAAATGTAAAAATGATATGTTATTTAAAACAGGTAGATTTGGAAATTTTTTCATGTGCATT
AATGATACATGTAAAAATACACGAAAAATTTTACCTAACGGTGAAATATCAGAACCAAAATTTGAACCTATTCCATTTCC
TGATATATTATGTGAAAAATCTAATACATGGTTTGTTTTAAGAGAGGGAATTTCTGGTATTTTTTTTGCTGCAAATACCT
TTCCTAAATCGCGTGAAACAAGATCTCCTTTTGTAGAAGAACTTGCTAGATTTGAATATTTATTACCAAAAAAATTACAC
TATTTAGCTAGCGCACCTCAAAGAGATAACAAAGGAAATAAAACTATAGTATGTTTTAATAAATTAAATAAACAGCAATA
TATTGCCTCTAAAAAAGAAGGAAAATTTACAGGTTGGGCAGCTTTTTTTATTGATAGAAAATGGTGTATTGTGAATAAAT
AA

Upstream 100 bases:

>100_bases
TAAACATTTAAAATATATATAAATTTTTTAATCTATTGTAAATTATAATTGTGACTTTGTCTTATTATTAAAGAGATTTT
GATATTATTGGATAAAAATT

Downstream 100 bases:

>100_bases
CATAAACTAATTAATTATTGAAAGAGTAACATTGACTTATTTTTTCAGTAATTAATCTCATAAATTTCGAACTACTAGTT
AAATTTGAAGTACAAAAATT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTALIQQMGINPYKNWKAEYHIL
PGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEVIGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQ
ARRFMDRIVGYMISPLLWKKISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTF
LLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMFLAQKLYEQGYITYIRTDSYF
LSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHEAIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERY
KSIKTTVMADVFKLQSNTKIVLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKG
IGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNLDQVSDKLINWKHLLNSFFDN
FSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKTAVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEE
KNFHLKNRCKKCNLVMDVYLINENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCI
NDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRETRSPFVEELARFEYLLPKKLH
YLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWAAFFIDRKWCIVNK

Sequences:

>Translated_853_residues
MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTALIQQMGINPYKNWKAEYHIL
PGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEVIGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQ
ARRFMDRIVGYMISPLLWKKISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTF
LLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMFLAQKLYEQGYITYIRTDSYF
LSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHEAIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERY
KSIKTTVMADVFKLQSNTKIVLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKG
IGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNLDQVSDKLINWKHLLNSFFDN
FSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKTAVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEE
KNFHLKNRCKKCNLVMDVYLINENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCI
NDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRETRSPFVEELARFEYLLPKKLH
YLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWAAFFIDRKWCIVNK
>Mature_853_residues
MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTALIQQMGINPYKNWKAEYHIL
PGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEVIGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQ
ARRFMDRIVGYMISPLLWKKISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTF
LLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMFLAQKLYEQGYITYIRTDSYF
LSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHEAIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERY
KSIKTTVMADVFKLQSNTKIVLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKG
IGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNLDQVSDKLINWKHLLNSFFDN
FSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKTAVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEE
KNFHLKNRCKKCNLVMDVYLINENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCI
NDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRETRSPFVEELARFEYLLPKKLH
YLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWAAFFIDRKWCIVNK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=562, Percent_Identity=23.1316725978648, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI4507635, Length=499, Percent_Identity=22.8456913827655, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1787529, Length=863, Percent_Identity=52.6071842410197, Blast_Score=975, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=649, Percent_Identity=21.1093990755008, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=644, Percent_Identity=22.6708074534161, Blast_Score=119, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=491, Percent_Identity=23.8289205702648, Blast_Score=108, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=478, Percent_Identity=24.2677824267782, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24640096, Length=566, Percent_Identity=22.4381625441696, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585251, Length=491, Percent_Identity=23.4215885947047, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_BUCAP (Q8K9P7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660620.1
- ProteinModelPortal:   Q8K9P7
- EnsemblBacteria:   EBBUCT00000000343
- GeneID:   1005477
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg273
- GeneTree:   EBGT00050000008158
- HOGENOM:   HBG512935
- OMA:   EEYWSID
- ProtClustDB:   PRK07561
- BioCyc:   BAPH198804:BUSG273-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:1.10.290.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 98817; Mature: 98817

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTA
CCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHH
LIQQMGINPYKNWKAEYHILPGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEV
HHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHH
IGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQARRFMDRIVGYMISPLLWKK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTF
HHCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCEEE
LLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMF
EECCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
LAQKLYEQGYITYIRTDSYFLSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHE
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
AIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERYKSIKTTVMADVFKLQSNTKI
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKG
EEEECHHHHHHCCCCCEEEEEEHHCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCC
IGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNL
CCCCHHHHHHHHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DQVSDKLINWKHLLNSFFDNFSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCHHHHCCCCCHHH
AVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEEKNFHLKNRCKKCNLVMDVYL
HHHHHHHHHHCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHCCCHHHHHHHCCEEEEEEE
INENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCI
ECCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCEEEEE
NDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRET
CCHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCC
RSPFVEELARFEYLLPKKLHYLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCEEEE
AFFIDRKWCIVNK
EEEEECCEEECCC
>Mature Secondary Structure
MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTA
CCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHH
LIQQMGINPYKNWKAEYHILPGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEV
HHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHH
IGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQARRFMDRIVGYMISPLLWKK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTF
HHCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCEEE
LLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMF
EECCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
LAQKLYEQGYITYIRTDSYFLSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHE
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
AIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERYKSIKTTVMADVFKLQSNTKI
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKG
EEEECHHHHHHCCCCCEEEEEEHHCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCC
IGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNL
CCCCHHHHHHHHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DQVSDKLINWKHLLNSFFDNFSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCHHHHCCCCCHHH
AVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEEKNFHLKNRCKKCNLVMDVYL
HHHHHHHHHHCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHCCCHHHHHHHCCEEEEEEE
INENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCI
ECCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCEEEEE
NDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRET
CCHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCC
RSPFVEELARFEYLLPKKLHYLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCEEEE
AFFIDRKWCIVNK
EEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438