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The map label for this gene is truA

Identifier: 21672480

GI number: 21672480

Start: 216323

End: 217114

Strand: Direct

Name: truA

Synonym: BUsg193

Alternate gene names: 21672480

Gene position: 216323-217114 (Clockwise)

Preceding gene: 21672476

Following gene: 21672481

Centisome position: 33.72

GC content: 28.16

Gene sequence:

>792_bases
ATGAAAGAAAAAAAAAATAAAAAATTTGCCTTAGGAGTAGAATACGATGGAAGTTATTATCATGGTTGGCAAAGTCAAAA
AGGACTTTCTACTATTCAATCAGAAATAGAGAAGGCTTTGTCTAAAATTGCAAATCATCAAGTGAACGTTATATGTGCTG
GTCGGACTGATTCCGGTGTTCATAGCATAGGACAAGTAATACATTTCACTAGTATTTCGTTTAGAAGTGATTTTTCTTGG
ATAGTGGGAGTGAATAGTTATTTATCTAAAAATATTTCTGTAAAATGGATTAAAGAAGTTTCAGAAAATTTCAATGCTCG
TTATAGTGCAATTTCTCGCTCTTACCGTTATATAATATATAATCATAAATGTCGTTCTGCTATTTTTCGAAATAGATCAA
ATCATGTTTATAAAGAATTAAATGTTGATAAGATGTATTTTGAAGCACAATCCTTATTAGGAGAACATGATTTTTCATCT
TTTCGATCCTCAGGCTGTCAATCACGTTCTCCTTTCAGAACAATTACTGATTTAAATATTCGGCGTTTAAAAAATTTAGT
TATTATTGATATTACTGCTAATTCTTTTTTATATCATATGGTTCGAAACATTGTTGGTTCATTGATTCAAATCAACGATA
ATACAAAAAAAAATTATATAAAAAAAATATTAGATAAAAAAGATAGAAACTATGCAGGTCCTACTGTTTCAGCTCGAGGG
TTATACTTATTTTTTGTAAAATATCCTATGTGTTTTAATTTGCCTATATTTAAGGATGATTTTATTATTTGA

Upstream 100 bases:

>100_bases
TAAATAATATTAACATAAACTTTAGTTAAATATATTCTATAGAAGTATGTTGATTTAAAAAATTATATTTTTTTATCTAT
AATTATAGGAAATGTAATAA

Downstream 100 bases:

>100_bases
AGAATAAAAATGCACGTCAGATTACTTTTTAGAATAGTTGACTTCTTAAATTTATCATTAATTATATTCAACAAGTTATG
TATATGCTTTTCAAATGAAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKEKKNKKFALGVEYDGSYYHGWQSQKGLSTIQSEIEKALSKIANHQVNVICAGRTDSGVHSIGQVIHFTSISFRSDFSW
IVGVNSYLSKNISVKWIKEVSENFNARYSAISRSYRYIIYNHKCRSAIFRNRSNHVYKELNVDKMYFEAQSLLGEHDFSS
FRSSGCQSRSPFRTITDLNIRRLKNLVIIDITANSFLYHMVRNIVGSLIQINDNTKKNYIKKILDKKDRNYAGPTVSARG
LYLFFVKYPMCFNLPIFKDDFII

Sequences:

>Translated_263_residues
MKEKKNKKFALGVEYDGSYYHGWQSQKGLSTIQSEIEKALSKIANHQVNVICAGRTDSGVHSIGQVIHFTSISFRSDFSW
IVGVNSYLSKNISVKWIKEVSENFNARYSAISRSYRYIIYNHKCRSAIFRNRSNHVYKELNVDKMYFEAQSLLGEHDFSS
FRSSGCQSRSPFRTITDLNIRRLKNLVIIDITANSFLYHMVRNIVGSLIQINDNTKKNYIKKILDKKDRNYAGPTVSARG
LYLFFVKYPMCFNLPIFKDDFII
>Mature_263_residues
MKEKKNKKFALGVEYDGSYYHGWQSQKGLSTIQSEIEKALSKIANHQVNVICAGRTDSGVHSIGQVIHFTSISFRSDFSW
IVGVNSYLSKNISVKWIKEVSENFNARYSAISRSYRYIIYNHKCRSAIFRNRSNHVYKELNVDKMYFEAQSLLGEHDFSS
FRSSGCQSRSPFRTITDLNIRRLKNLVIIDITANSFLYHMVRNIVGSLIQINDNTKKNYIKKILDKKDRNYAGPTVSARG
LYLFFVKYPMCFNLPIFKDDFII

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=48.995983935743, Blast_Score=281, Evalue=3e-77,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=28.0936454849498, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362146, Length=275, Percent_Identity=24.7272727272727, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648455, Length=275, Percent_Identity=24.7272727272727, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BUCAP (Q8K9U4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660547.1
- ProteinModelPortal:   Q8K9U4
- SMR:   Q8K9U4
- EnsemblBacteria:   EBBUCT00000000328
- GeneID:   1005390
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg193
- GeneTree:   EBGT00050000008034
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   BAPH198804:BUSG193-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30496; Mature: 30496

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: ACT_SITE 57-57 BINDING 115-115 BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKKNKKFALGVEYDGSYYHGWQSQKGLSTIQSEIEKALSKIANHQVNVICAGRTDSGV
CCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
HSIGQVIHFTSISFRSDFSWIVGVNSYLSKNISVKWIKEVSENFNARYSAISRSYRYIIY
HHHHHHHHHEEEECCCCCCEEEECHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCEEEEEE
NHKCRSAIFRNRSNHVYKELNVDKMYFEAQSLLGEHDFSSFRSSGCQSRSPFRTITDLNI
CCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHCCHH
RRLKNLVIIDITANSFLYHMVRNIVGSLIQINDNTKKNYIKKILDKKDRNYAGPTVSARG
HHHCCEEEEEEECHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
LYLFFVKYPMCFNLPIFKDDFII
EEEEEEECCCEECCCCCCCCCCC
>Mature Secondary Structure
MKEKKNKKFALGVEYDGSYYHGWQSQKGLSTIQSEIEKALSKIANHQVNVICAGRTDSGV
CCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
HSIGQVIHFTSISFRSDFSWIVGVNSYLSKNISVKWIKEVSENFNARYSAISRSYRYIIY
HHHHHHHHHEEEECCCCCCEEEECHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCEEEEEE
NHKCRSAIFRNRSNHVYKELNVDKMYFEAQSLLGEHDFSSFRSSGCQSRSPFRTITDLNI
CCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHCCHH
RRLKNLVIIDITANSFLYHMVRNIVGSLIQINDNTKKNYIKKILDKKDRNYAGPTVSARG
HHHCCEEEEEEECHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
LYLFFVKYPMCFNLPIFKDDFII
EEEEEEECCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438