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The map label for this gene is ppnK

Identifier: 21672466

GI number: 21672466

Start: 202048

End: 202926

Strand: Direct

Name: ppnK

Synonym: BUsg179

Alternate gene names: 21672466

Gene position: 202048-202926 (Clockwise)

Preceding gene: 21672463

Following gene: 21672467

Centisome position: 31.5

GC content: 26.96

Gene sequence:

>879_bases
ATGAAACAATATTTTAATTGTATTGGTATTGTTGGACGTCCGCGTCATTCAACTGCATTAATAACACATGAAATACTTTA
TAAGTGGTTAATTAAAAAAGGTTATCAAGTTTTTATTGAATATAATATTTCTAAAAAGCTAAATTTAAAAAACCCTAAAA
CTGCGACATTAATAGAAATTGGTCGATTATGTGATTTAGCAATTGTAATAGGCGGAGATGGAAATTTATTATTTACAGCT
CGTATTTTATCATATTTTAATATTAAAATCATTGGTATTAATTGTGGAAATTTAGGTTTTCTTACAGATTTGAATCCAGA
TAATAAATTTAAAAAACTTTCAGAAGTTTTGTCTGGTAAATATTTCGTAGAAAATCGATTCTTATTAGATGTAATGATTT
ATAAAAAAGAACAAGTTTCTAAATCTAGTATTGCAATTAATGAAGTTGTTTTGCATCCTAAAAACGTAGCGCATATGATA
GAATTTGAAGTTTATATTAATGACAATTTTGCTTTTTCTCAACGTTCAGATGGCTTGATTATTTCAACTCCAACAGGTTC
AACAGGTTATTCACTTTCAGCTGGAGGACCTATTATAGAAACTTCCTTAGAATCTATTTTGTTAGTACCTATGTTCCCCC
ACACTTTATCTGCGCGTCCTTTATTAATTCGCAGTGATAGTGTAATTCGTTTGAGATTTTCTGATATAGAAACTGATTTA
AAAATTAGTTGTGATAGTCAAATAGTTTTACCTGTTAAGAAAAAAGAATATGTATTTATTCGTCGCAGTAACTATTATTT
GAATCTCATACATCCTAAAAGTTATAATTATTTTGAAACTCTAACATCTAAATTAAATTGGTCTAAAAAATTTTTTTAG

Upstream 100 bases:

>100_bases
TACCATTTTAAATGTTATTAATTAAATGTAGAGTTTTTAATATGTTTTTCAAGGTAATTTTTTTATTAAAAATATTTTTT
TTATTGGGAAATTTAATGCA

Downstream 100 bases:

>100_bases
ATTATTAAAGATTTTTATAAAATTTTTTTATAGTTAAAGATTGTTATTAACAATATTTCATATAAAATATTATAGGTAGT
GATTTTTTTATGAGTAATCA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKQYFNCIGIVGRPRHSTALITHEILYKWLIKKGYQVFIEYNISKKLNLKNPKTATLIEIGRLCDLAIVIGGDGNLLFTA
RILSYFNIKIIGINCGNLGFLTDLNPDNKFKKLSEVLSGKYFVENRFLLDVMIYKKEQVSKSSIAINEVVLHPKNVAHMI
EFEVYINDNFAFSQRSDGLIISTPTGSTGYSLSAGGPIIETSLESILLVPMFPHTLSARPLLIRSDSVIRLRFSDIETDL
KISCDSQIVLPVKKKEYVFIRRSNYYLNLIHPKSYNYFETLTSKLNWSKKFF

Sequences:

>Translated_292_residues
MKQYFNCIGIVGRPRHSTALITHEILYKWLIKKGYQVFIEYNISKKLNLKNPKTATLIEIGRLCDLAIVIGGDGNLLFTA
RILSYFNIKIIGINCGNLGFLTDLNPDNKFKKLSEVLSGKYFVENRFLLDVMIYKKEQVSKSSIAINEVVLHPKNVAHMI
EFEVYINDNFAFSQRSDGLIISTPTGSTGYSLSAGGPIIETSLESILLVPMFPHTLSARPLLIRSDSVIRLRFSDIETDL
KISCDSQIVLPVKKKEYVFIRRSNYYLNLIHPKSYNYFETLTSKLNWSKKFF
>Mature_292_residues
MKQYFNCIGIVGRPRHSTALITHEILYKWLIKKGYQVFIEYNISKKLNLKNPKTATLIEIGRLCDLAIVIGGDGNLLFTA
RILSYFNIKIIGINCGNLGFLTDLNPDNKFKKLSEVLSGKYFVENRFLLDVMIYKKEQVSKSSIAINEVVLHPKNVAHMI
EFEVYINDNFAFSQRSDGLIISTPTGSTGYSLSAGGPIIETSLESILLVPMFPHTLSARPLLIRSDSVIRLRFSDIETDL
KISCDSQIVLPVKKKEYVFIRRSNYYLNLIHPKSYNYFETLTSKLNWSKKFF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=65.4109589041096, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=29.7520661157025, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325068, Length=178, Percent_Identity=34.2696629213483, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322509, Length=241, Percent_Identity=26.5560165975104, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573830, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28573832, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28573826, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28573828, Length=188, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI161077047, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24653422, Length=270, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129957, Length=270, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363323, Length=270, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653424, Length=270, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363321, Length=270, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_BUCAP (Q8K9V8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660533.1
- ProteinModelPortal:   Q8K9V8
- SMR:   Q8K9V8
- EnsemblBacteria:   EBBUCT00000000531
- GeneID:   1005376
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg179
- GeneTree:   EBGT00050000007815
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK03378
- BioCyc:   BAPH198804:BUSG179-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33366; Mature: 33366

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYFNCIGIVGRPRHSTALITHEILYKWLIKKGYQVFIEYNISKKLNLKNPKTATLIEI
CCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEEEEECCCEEECCCCCEEHEEEC
GRLCDLAIVIGGDGNLLFTARILSYFNIKIIGINCGNLGFLTDLNPDNKFKKLSEVLSGK
CCEEEEEEEEECCCCEEEEEEEEHEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHCCC
YFVENRFLLDVMIYKKEQVSKSSIAINEVVLHPKNVAHMIEFEVYINDNFAFSQRSDGLI
EEECCEEEEEEEEECHHHCCCCCEEEEEEEECCCCCEEEEEEEEEECCCEEECCCCCCEE
ISTPTGSTGYSLSAGGPIIETSLESILLVPMFPHTLSARPLLIRSDSVIRLRFSDIETDL
EECCCCCCCEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCE
KISCDSQIVLPVKKKEYVFIRRSNYYLNLIHPKSYNYFETLTSKLNWSKKFF
EEECCCEEEEEECCCEEEEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKQYFNCIGIVGRPRHSTALITHEILYKWLIKKGYQVFIEYNISKKLNLKNPKTATLIEI
CCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEEEEECCCEEECCCCCEEHEEEC
GRLCDLAIVIGGDGNLLFTARILSYFNIKIIGINCGNLGFLTDLNPDNKFKKLSEVLSGK
CCEEEEEEEEECCCCEEEEEEEEHEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHCCC
YFVENRFLLDVMIYKKEQVSKSSIAINEVVLHPKNVAHMIEFEVYINDNFAFSQRSDGLI
EEECCEEEEEEEEECHHHCCCCCEEEEEEEECCCCCEEEEEEEEEECCCEEECCCCCCEE
ISTPTGSTGYSLSAGGPIIETSLESILLVPMFPHTLSARPLLIRSDSVIRLRFSDIETDL
EECCCCCCCEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCE
KISCDSQIVLPVKKKEYVFIRRSNYYLNLIHPKSYNYFETLTSKLNWSKKFF
EEECCCEEEEEECCCEEEEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438