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The map label for this gene is nuoE

Identifier: 21672440

GI number: 21672440

Start: 168171

End: 168677

Strand: Direct

Name: nuoE

Synonym: BUsg150

Alternate gene names: 21672440

Gene position: 168171-168677 (Clockwise)

Preceding gene: 21672439

Following gene: 21672441

Centisome position: 26.22

GC content: 25.84

Gene sequence:

>507_bases
ATGGATAAAAAAAAAAAAATAAAAGAAATCTCTACAACATTTACTTTAACTACTTTTGAAATTAATGAAATAGAAAAGCA
AAAAAAATATTATGAAAATTCTAGATCAGTTTCAATAGAAGCATTAAAAATTGTTCAAAAAAAACGAGGATGGGTCTGTG
ATCAAGCAATTATAGAAATTGCAAACATACTCAATCTTAATCCAAGTGAAGTAGAAAGTGTAGCTACTTTTTATAGTCAA
ATTTATCGTCAACCTGTAGGTCGTAACGTCATAAGATATTGTGATAGTGTGGTTTGTTATTTAACTGGTTATGAAAAAAT
AAAAAAATTTTTAGAAGACTATTTATTTATTAAAATAGGTAATACTACTGTAGATGGTAGATTTACCTTATTGCCAGTTT
GTTGTTTAGGAAATTGTGATAAAGCTCCTACCATAATGATTAATGAAGATTTATATTCTCACCTTAATCCAGATTTAATA
CCAAATTTGCTGGAATTATATAAATGA

Upstream 100 bases:

>100_bases
TACAGCAAATACCTTCAGTTATTCGTGGAAGTTTAATATCAGATTTAATTGTATATTTAGGCAGTATAGATTTTGTCATG
TCAGATGTGGACAGATAATT

Downstream 100 bases:

>100_bases
ATAATATTATCCCCACTGAGGAGACACATCCGTTAACATGGAGATTAAGAAATGATCGAGAAACAGTTTGGATTGAAGAA
TATTGTAAAAAAAATGGTTA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MDKKKKIKEISTTFTLTTFEINEIEKQKKYYENSRSVSIEALKIVQKKRGWVCDQAIIEIANILNLNPSEVESVATFYSQ
IYRQPVGRNVIRYCDSVVCYLTGYEKIKKFLEDYLFIKIGNTTVDGRFTLLPVCCLGNCDKAPTIMINEDLYSHLNPDLI
PNLLELYK

Sequences:

>Translated_168_residues
MDKKKKIKEISTTFTLTTFEINEIEKQKKYYENSRSVSIEALKIVQKKRGWVCDQAIIEIANILNLNPSEVESVATFYSQ
IYRQPVGRNVIRYCDSVVCYLTGYEKIKKFLEDYLFIKIGNTTVDGRFTLLPVCCLGNCDKAPTIMINEDLYSHLNPDLI
PNLLELYK
>Mature_168_residues
MDKKKKIKEISTTFTLTTFEINEIEKQKKYYENSRSVSIEALKIVQKKRGWVCDQAIIEIANILNLNPSEVESVATFYSQ
IYRQPVGRNVIRYCDSVVCYLTGYEKIKKFLEDYLFIKIGNTTVDGRFTLLPVCCLGNCDKAPTIMINEDLYSHLNPDLI
PNLLELYK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family

Homologues:

Organism=Homo sapiens, GI222080062, Length=146, Percent_Identity=35.6164383561644, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1788621, Length=158, Percent_Identity=61.3924050632911, Blast_Score=213, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17561328, Length=137, Percent_Identity=38.6861313868613, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI18859877, Length=123, Percent_Identity=37.3983739837398, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355353, Length=123, Percent_Identity=32.520325203252, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOE_BUCAP (Q8K9Y4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660507.1
- ProteinModelPortal:   Q8K9Y4
- SMR:   Q8K9Y4
- EnsemblBacteria:   EBBUCT00000000393
- GeneID:   1005967
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg150
- GeneTree:   EBGT00050000008166
- HOGENOM:   HBG616790
- OMA:   IVQKERG
- ProtClustDB:   PRK07539
- BioCyc:   BAPH198804:BUSG150-MONOMER
- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PANTHER:   PTHR10371
- PIRSF:   PIRSF000216
- TIGRFAMs:   TIGR01958

Pfam domain/function: PF01257 Complex1_24kDa; SSF52833 Thiordxn-like_fd

EC number: =1.6.99.5

Molecular weight: Translated: 19463; Mature: 19463

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKKIKEISTTFTLTTFEINEIEKQKKYYENSRSVSIEALKIVQKKRGWVCDQAIIEI
CCHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
ANILNLNPSEVESVATFYSQIYRQPVGRNVIRYCDSVVCYLTGYEKIKKFLEDYLFIKIG
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEC
NTTVDGRFTLLPVCCLGNCDKAPTIMINEDLYSHLNPDLIPNLLELYK
CEEECCCCEEEHHHHCCCCCCCCEEEECHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MDKKKKIKEISTTFTLTTFEINEIEKQKKYYENSRSVSIEALKIVQKKRGWVCDQAIIEI
CCHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
ANILNLNPSEVESVATFYSQIYRQPVGRNVIRYCDSVVCYLTGYEKIKKFLEDYLFIKIG
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEC
NTTVDGRFTLLPVCCLGNCDKAPTIMINEDLYSHLNPDLIPNLLELYK
CEEECCCCEEEHHHHCCCCCCCCEEEECHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438