Click here to switch to the map view.

The map label for this gene is nuoF

Identifier: 21672441

GI number: 21672441

Start: 168674

End: 170017

Strand: Direct

Name: nuoF

Synonym: BUsg151

Alternate gene names: 21672441

Gene position: 168674-170017 (Clockwise)

Preceding gene: 21672440

Following gene: 21672442

Centisome position: 26.3

GC content: 30.73

Gene sequence:

>1344_bases
ATGAATAATATTATCCCCACTGAGGAGACACATCCGTTAACATGGAGATTAAGAAATGATCGAGAAACAGTTTGGATTGA
AGAATATTGTAAAAAAAATGGTTATTTTAGTTTAAAAAAAGCATTAAAAACTATGTGTCCAGAAGATGTTGTACATTTGA
TAAAAGAATCAGGTTTAAAAGGAAGAGGAGGAGCTGGTTTTTCTACAGGTTTAAAATGGAGTTTAATGTCTAAAGACAAT
AGCTCTAAAATTCGTTATTTATTATGCAACGCTGATGAAATGGAACCTGGAACTTATAAAGATAGATTTTTAATGGAAAA
TATACCTCATCAATTAATTGAAGGAATGTTATTATCAGCGTTTGCCTTAAATGTTTCTCGTGGTTATATTTTTTTACGGG
GTGAATATATTAAAGCTGAATATATTTTAAAAAAATCTATACAAGAAGCAATTAATTTTGGTTTTATTGGTTCTAATATT
TTAAATAGTGGTTTTAATTTTGAACTTTTTTTACATACTGGAGCTGGTCGTTATATTTGTGGCGAAGAAACTGCTTTAAT
TAATTCGCTAGAGGGACGTAGAGCTAATCCAAGAGCTAAGCCACCTTTTCCTGCAGTATTTGGGCTATGGGGAAAACCTA
CTTGTGTTAACAATGTTGAAACATTATCTAATGTTCCTTCTATTGTATTACATGGTGTTAATTGGTATAAAAAACTATCT
AAAAGTACTGATACTACTGGTACTAAGTTAATGGGATTTTCAGGAAAAGTAAATAATCCTGGTGTTTGGGAATTACCTTT
TGGAACTACTGCACGTGAAATTTTAGAAGATTATGCACGTGGAATGAAATCAGGTTTATTTTTAAAATCTTGGCAACCTG
GTGGAGCAGGAACAGATTTTCTCATTGAAAAACATTTAGATTTACCGATGGATTTTACAAGTATTGCAAAAGCCGGAAGT
CGTTTAGGAACAGCTATTGCTATGGCTGTAGATAATAAAACTAATATGATTTCTTTAGTTTGCAATATAGAAAAGTTTTT
TTCTCGTGAATCATGTGGTTTATGTACACCATGCAGAGAAGGATTACCCTGGATTGTAAAGATATTAGAAAGTTTAGAGA
AAAAAGAAGGTCATAAAAATGATGTCAAAAATTTAGAGAGGTTATGTTTAGATTTAAGTCCAGGAAAAACATTTTGTGCT
CATGCACCTGGTGCAGTAGAACCTTTACAAAGTGCTATAAAATATTTTCGTTTAGAGTTTGAAGCTGGTATTAGCATAAA
AAAATTAAAAAAATGTTCAAATATTCTTGGAATTCAATCAAATGAATTTACTTCAAAAGTTTAA

Upstream 100 bases:

>100_bases
GTTTAGGAAATTGTGATAAAGCTCCTACCATAATGATTAATGAAGATTTATATTCTCACCTTAATCCAGATTTAATACCA
AATTTGCTGGAATTATATAA

Downstream 100 bases:

>100_bases
TTTTTTATATAAATAATTTCTAATATAAAGATTTTTATAAAAATAAAAGTTTTTTTTGGAACTCTTCTATTATGGCTAAA
ATTTATGTAGATGGAAAAGC

Product: NADH dehydrogenase I chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNNIIPTEETHPLTWRLRNDRETVWIEEYCKKNGYFSLKKALKTMCPEDVVHLIKESGLKGRGGAGFSTGLKWSLMSKDN
SSKIRYLLCNADEMEPGTYKDRFLMENIPHQLIEGMLLSAFALNVSRGYIFLRGEYIKAEYILKKSIQEAINFGFIGSNI
LNSGFNFELFLHTGAGRYICGEETALINSLEGRRANPRAKPPFPAVFGLWGKPTCVNNVETLSNVPSIVLHGVNWYKKLS
KSTDTTGTKLMGFSGKVNNPGVWELPFGTTAREILEDYARGMKSGLFLKSWQPGGAGTDFLIEKHLDLPMDFTSIAKAGS
RLGTAIAMAVDNKTNMISLVCNIEKFFSRESCGLCTPCREGLPWIVKILESLEKKEGHKNDVKNLERLCLDLSPGKTFCA
HAPGAVEPLQSAIKYFRLEFEAGISIKKLKKCSNILGIQSNEFTSKV

Sequences:

>Translated_447_residues
MNNIIPTEETHPLTWRLRNDRETVWIEEYCKKNGYFSLKKALKTMCPEDVVHLIKESGLKGRGGAGFSTGLKWSLMSKDN
SSKIRYLLCNADEMEPGTYKDRFLMENIPHQLIEGMLLSAFALNVSRGYIFLRGEYIKAEYILKKSIQEAINFGFIGSNI
LNSGFNFELFLHTGAGRYICGEETALINSLEGRRANPRAKPPFPAVFGLWGKPTCVNNVETLSNVPSIVLHGVNWYKKLS
KSTDTTGTKLMGFSGKVNNPGVWELPFGTTAREILEDYARGMKSGLFLKSWQPGGAGTDFLIEKHLDLPMDFTSIAKAGS
RLGTAIAMAVDNKTNMISLVCNIEKFFSRESCGLCTPCREGLPWIVKILESLEKKEGHKNDVKNLERLCLDLSPGKTFCA
HAPGAVEPLQSAIKYFRLEFEAGISIKKLKKCSNILGIQSNEFTSKV
>Mature_447_residues
MNNIIPTEETHPLTWRLRNDRETVWIEEYCKKNGYFSLKKALKTMCPEDVVHLIKESGLKGRGGAGFSTGLKWSLMSKDN
SSKIRYLLCNADEMEPGTYKDRFLMENIPHQLIEGMLLSAFALNVSRGYIFLRGEYIKAEYILKKSIQEAINFGFIGSNI
LNSGFNFELFLHTGAGRYICGEETALINSLEGRRANPRAKPPFPAVFGLWGKPTCVNNVETLSNVPSIVLHGVNWYKKLS
KSTDTTGTKLMGFSGKVNNPGVWELPFGTTAREILEDYARGMKSGLFLKSWQPGGAGTDFLIEKHLDLPMDFTSIAKAGS
RLGTAIAMAVDNKTNMISLVCNIEKFFSRESCGLCTPCREGLPWIVKILESLEKKEGHKNDVKNLERLCLDLSPGKTFCA
HAPGAVEPLQSAIKYFRLEFEAGISIKKLKKCSNILGIQSNEFTSKV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family

Homologues:

Organism=Homo sapiens, GI260656005, Length=378, Percent_Identity=41.2698412698413, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI20149568, Length=378, Percent_Identity=41.2698412698413, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1788620, Length=441, Percent_Identity=70.9750566893424, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=370, Percent_Identity=40.8108108108108, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI19920794, Length=388, Percent_Identity=41.4948453608247, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI45550452, Length=396, Percent_Identity=39.1414141414141, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24653819, Length=378, Percent_Identity=33.0687830687831, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=31.1614730878187, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOF_BUCAP (Q8K9Y3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660508.1
- ProteinModelPortal:   Q8K9Y3
- SMR:   Q8K9Y3
- EnsemblBacteria:   EBBUCT00000000083
- GeneID:   1005968
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg151
- GeneTree:   EBGT00050000007982
- HOGENOM:   HBG669338
- OMA:   LHTGAGR
- ProtClustDB:   CLSK315384
- BioCyc:   BAPH198804:BUSG151-MONOMER
- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554
- SMART:   SM00928
- TIGRFAMs:   TIGR01959

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB

EC number: =1.6.99.5

Molecular weight: Translated: 49865; Mature: 49865

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00644 COMPLEX1_51K_1; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIPTEETHPLTWRLRNDRETVWIEEYCKKNGYFSLKKALKTMCPEDVVHLIKESGLK
CCCCCCCCCCCCEEEEECCCCCEEEHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMSKDNSSKIRYLLCNADEMEPGTYKDRFLMENIPHQLIEGMLLSA
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
FALNVSRGYIFLRGEYIKAEYILKKSIQEAINFGFIGSNILNSGFNFELFLHTGAGRYIC
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRAKPPFPAVFGLWGKPTCVNNVETLSNVPSIVLHGVNWYKKLS
CCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCHHHHHCCHHHHHHH
KSTDTTGTKLMGFSGKVNNPGVWELPFGTTAREILEDYARGMKSGLFLKSWQPGGAGTDF
CCCCCCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
LIEKHLDLPMDFTSIAKAGSRLGTAIAMAVDNKTNMISLVCNIEKFFSRESCGLCTPCRE
HHHHCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECHHHHHCCCCCCCCCHHHC
GLPWIVKILESLEKKEGHKNDVKNLERLCLDLSPGKTFCAHAPGAVEPLQSAIKYFRLEF
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCEECCCCCHHHHHHHHHHHHEEHH
EAGISIKKLKKCSNILGIQSNEFTSKV
HCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNNIIPTEETHPLTWRLRNDRETVWIEEYCKKNGYFSLKKALKTMCPEDVVHLIKESGLK
CCCCCCCCCCCCEEEEECCCCCEEEHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMSKDNSSKIRYLLCNADEMEPGTYKDRFLMENIPHQLIEGMLLSA
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
FALNVSRGYIFLRGEYIKAEYILKKSIQEAINFGFIGSNILNSGFNFELFLHTGAGRYIC
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRAKPPFPAVFGLWGKPTCVNNVETLSNVPSIVLHGVNWYKKLS
CCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCHHHHHCCHHHHHHH
KSTDTTGTKLMGFSGKVNNPGVWELPFGTTAREILEDYARGMKSGLFLKSWQPGGAGTDF
CCCCCCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
LIEKHLDLPMDFTSIAKAGSRLGTAIAMAVDNKTNMISLVCNIEKFFSRESCGLCTPCRE
HHHHCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECHHHHHCCCCCCCCCHHHC
GLPWIVKILESLEKKEGHKNDVKNLERLCLDLSPGKTFCAHAPGAVEPLQSAIKYFRLEF
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCEECCCCCHHHHHHHHHHHHEEHH
EAGISIKKLKKCSNILGIQSNEFTSKV
HCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438