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The map label for this gene is folE

Identifier: 21672419

GI number: 21672419

Start: 143817

End: 144473

Strand: Direct

Name: folE

Synonym: BUsg129

Alternate gene names: 21672419

Gene position: 143817-144473 (Clockwise)

Preceding gene: 21672416

Following gene: 21672420

Centisome position: 22.42

GC content: 22.68

Gene sequence:

>657_bases
ATGATTAAAATCACTAAAGAAGCTAATATTGCACGTAATGTTTTATTATTAAAAGGTTTAGAAAATCCTATTTTAAAAGA
ATACAATGATATAAAAGAAAAAGAAAGAGAATTATTAATTGCAGAATATTTATATAAAATTATGTCTTTACTAAATTTAG
ATCTAAAAAACGAAAGTTTAAAAGATACACCTATTCGAATATCAAAAATGTATGTTAATGAAATATTTTCTGGTTTAGAT
TATAAAAATTTTCCAAAAATTATGTTGATGGATAATAAAATAAAATTTCATGAAATGATTATAGTACGCGATATTCTATT
AATAAGCACTTGCGAACATCATTTTATTACTATTAATGGACAAGCTACTATTGCATATATTCCTGAGAATAAAATTATTG
GTTTATCTAAAATAAATAGAATTGCACAATTTTTTGCAAAAAGACCTCAAATTCAAGAACGTTTAACAAAACAAATACTT
CTAGTTCTGCAAGTTTTATTACAAACTAAAAATGTTGCAATAATTATTCATATGGATCATTTTTGTGTTAAAGCTCGAGG
AGTATGTGATACCAGTAGTAGTACTGTTACTTCATGTTTAGATGGTTTATTTAAATCTGATAAAAATATTCGAGAAGAAT
TTTTTTTTAAAAAATAA

Upstream 100 bases:

>100_bases
ATGATTTTTTTACGTATAAATGTTTTAGTTATAAATCAGTTTAAATGGTTTTCACATGTAAAAATAAAAAGTGTATGATA
TTTAATTTAAGGAAAAAATT

Downstream 100 bases:

>100_bases
AAAATAGGAATAAATATATCTATTTTATTGATTAAAACAATTTTTCTCAGTATGATATTAATTATTCATTAAAATATATA
ATTTTTAAAAAAATCAATTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIKITKEANIARNVLLLKGLENPILKEYNDIKEKERELLIAEYLYKIMSLLNLDLKNESLKDTPIRISKMYVNEIFSGLD
YKNFPKIMLMDNKIKFHEMIIVRDILLISTCEHHFITINGQATIAYIPENKIIGLSKINRIAQFFAKRPQIQERLTKQIL
LVLQVLLQTKNVAIIIHMDHFCVKARGVCDTSSSTVTSCLDGLFKSDKNIREEFFFKK

Sequences:

>Translated_218_residues
MIKITKEANIARNVLLLKGLENPILKEYNDIKEKERELLIAEYLYKIMSLLNLDLKNESLKDTPIRISKMYVNEIFSGLD
YKNFPKIMLMDNKIKFHEMIIVRDILLISTCEHHFITINGQATIAYIPENKIIGLSKINRIAQFFAKRPQIQERLTKQIL
LVLQVLLQTKNVAIIIHMDHFCVKARGVCDTSSSTVTSCLDGLFKSDKNIREEFFFKK
>Mature_218_residues
MIKITKEANIARNVLLLKGLENPILKEYNDIKEKERELLIAEYLYKIMSLLNLDLKNESLKDTPIRISKMYVNEIFSGLD
YKNFPKIMLMDNKIKFHEMIIVRDILLISTCEHHFITINGQATIAYIPENKIIGLSKINRIAQFFAKRPQIQERLTKQIL
LVLQVLLQTKNVAIIIHMDHFCVKARGVCDTSSSTVTSCLDGLFKSDKNIREEFFFKK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=216, Percent_Identity=34.2592592592593, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4503949, Length=216, Percent_Identity=34.2592592592593, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI66932972, Length=172, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI66932970, Length=172, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1788476, Length=215, Percent_Identity=58.1395348837209, Blast_Score=262, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17560486, Length=118, Percent_Identity=46.6101694915254, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=40.9836065573771, Blast_Score=135, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24656782, Length=118, Percent_Identity=45.7627118644068, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656791, Length=118, Percent_Identity=45.7627118644068, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656796, Length=118, Percent_Identity=45.7627118644068, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_BUCAP (Q8KA05)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660486.1
- ProteinModelPortal:   Q8KA05
- SMR:   Q8KA05
- EnsemblBacteria:   EBBUCT00000000357
- GeneID:   1005946
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg129
- HOGENOM:   HBG370191
- OMA:   MYVEEIF
- ProtClustDB:   PRK09347
- BioCyc:   BAPH198804:BUSG129-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25363; Mature: 25363

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITKEANIARNVLLLKGLENPILKEYNDIKEKERELLIAEYLYKIMSLLNLDLKNESL
CEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDTPIRISKMYVNEIFSGLDYKNFPKIMLMDNKIKFHEMIIVRDILLISTCEHHFITING
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
QATIAYIPENKIIGLSKINRIAQFFAKRPQIQERLTKQILLVLQVLLQTKNVAIIIHMDH
CEEEEECCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
FCVKARGVCDTSSSTVTSCLDGLFKSDKNIREEFFFKK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MIKITKEANIARNVLLLKGLENPILKEYNDIKEKERELLIAEYLYKIMSLLNLDLKNESL
CEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDTPIRISKMYVNEIFSGLDYKNFPKIMLMDNKIKFHEMIIVRDILLISTCEHHFITING
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
QATIAYIPENKIIGLSKINRIAQFFAKRPQIQERLTKQILLVLQVLLQTKNVAIIIHMDH
CEEEEECCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
FCVKARGVCDTSSSTVTSCLDGLFKSDKNIREEFFFKK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438