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The map label for this gene is nfo

Identifier: 21672420

GI number: 21672420

Start: 144620

End: 145459

Strand: Direct

Name: nfo

Synonym: BUsg130

Alternate gene names: 21672420

Gene position: 144620-145459 (Clockwise)

Preceding gene: 21672419

Following gene: 21672421

Centisome position: 22.55

GC content: 26.07

Gene sequence:

>840_bases
ATGAACTATATTGGTGCACATGTTAGTTCTTCTGGTGGTTTAGAAAAAGCAGTCTTTCGTGCTTTTCAAATAAAAGCAAC
AACTTTTTCATTTTTTACTAAAAATCAACGTCAATGGATTTCTCTTCCCTTACAAAAAAAAAATATAGAGGATTTTAAAA
AAGCTTGTATTAAATATAATTTTTCACCGGAAAAAATTTTACCTCATAGTAGTTATTTAATTAATTTAGGTCATCCTATT
GATTTTTTTTTAGAAAAATCTCGGGTTGCTTTTATTGATGAAATAGTTCGTTGTGACCAACTTGGTTTACGTTTTTTAAA
TTTTCATCCCGGTAGTCATTTGAATAAAATTTCTGAAGTGACTTGCTTATCAAGAATTTCTGAATCTATTAATATAGCCT
TAGAAAAAACAAAGAACGTAGTTGCTGTAATAGAAAACACTGCAGGTCAAGGAACTAATATTGGTTATAGATTTGAACAT
TTATATGAAATTATTAAAAAAATTGATGATAAATCTAGAATCGGAGTTTGTATCGATACGTGTCATTTATTTGCTTCAGG
ATATGATTTAAGAACAAAAATAGATTATGAAAGTACATTTAATAAATTTTTTGATTTAATAGAAATGAAGTATTTAAAGG
GTTTCCATTTGAATGATTCTAAAAAACAGTTTAATAGTCGTGTAGATCGGCATGAAAATTTAGGTTTAGGTGAAATTGGA
AAATCAGTTTTTAAATGGATTATAAAAAATAAAAATTTTCATAATATTCCAATGATATTAGAAACAATTAATCCAAAACT
ATGGGAAAAAGAAATTGATTGGTTAAGATCATTACAATGA

Upstream 100 bases:

>100_bases
TCAGTATGATATTAATTATTCATTAAAATATATAATTTTTAAAAAAATCAATTTTTTCTCCTTGCTAAAAAATATAAAAA
TCTTTACGGAAAAGAAAAAT

Downstream 100 bases:

>100_bases
AAATCTAACTAATTTTTTTATTAAAATCAGAGAGAGTATCCCCTATGTTTATAGTCAAAGCGGAAATAAGAGATAAAAAA
GGTAAGAGTTTTAGTAGAAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYIGAHVSSSGGLEKAVFRAFQIKATTFSFFTKNQRQWISLPLQKKNIEDFKKACIKYNFSPEKILPHSSYLINLGHPI
DFFLEKSRVAFIDEIVRCDQLGLRFLNFHPGSHLNKISEVTCLSRISESINIALEKTKNVVAVIENTAGQGTNIGYRFEH
LYEIIKKIDDKSRIGVCIDTCHLFASGYDLRTKIDYESTFNKFFDLIEMKYLKGFHLNDSKKQFNSRVDRHENLGLGEIG
KSVFKWIIKNKNFHNIPMILETINPKLWEKEIDWLRSLQ

Sequences:

>Translated_279_residues
MNYIGAHVSSSGGLEKAVFRAFQIKATTFSFFTKNQRQWISLPLQKKNIEDFKKACIKYNFSPEKILPHSSYLINLGHPI
DFFLEKSRVAFIDEIVRCDQLGLRFLNFHPGSHLNKISEVTCLSRISESINIALEKTKNVVAVIENTAGQGTNIGYRFEH
LYEIIKKIDDKSRIGVCIDTCHLFASGYDLRTKIDYESTFNKFFDLIEMKYLKGFHLNDSKKQFNSRVDRHENLGLGEIG
KSVFKWIIKNKNFHNIPMILETINPKLWEKEIDWLRSLQ
>Mature_279_residues
MNYIGAHVSSSGGLEKAVFRAFQIKATTFSFFTKNQRQWISLPLQKKNIEDFKKACIKYNFSPEKILPHSSYLINLGHPI
DFFLEKSRVAFIDEIVRCDQLGLRFLNFHPGSHLNKISEVTCLSRISESINIALEKTKNVVAVIENTAGQGTNIGYRFEH
LYEIIKKIDDKSRIGVCIDTCHLFASGYDLRTKIDYESTFNKFFDLIEMKYLKGFHLNDSKKQFNSRVDRHENLGLGEIG
KSVFKWIIKNKNFHNIPMILETINPKLWEKEIDWLRSLQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=279, Percent_Identity=61.6487455197133, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17531193, Length=267, Percent_Identity=44.9438202247191, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6322735, Length=279, Percent_Identity=41.5770609318996, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BUCAP (P59058)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660487.1
- ProteinModelPortal:   P59058
- SMR:   P59058
- EnsemblBacteria:   EBBUCT00000000490
- GeneID:   1005947
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg130
- GeneTree:   EBGT00050000008125
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   BAPH198804:BUSG130-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32418; Mature: 32418

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIGAHVSSSGGLEKAVFRAFQIKATTFSFFTKNQRQWISLPLQKKNIEDFKKACIKYN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHCCCCCEEECCCCCCCHHHHHHHHHCCC
FSPEKILPHSSYLINLGHPIDFFLEKSRVAFIDEIVRCDQLGLRFLNFHPGSHLNKISEV
CCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
TCLSRISESINIALEKTKNVVAVIENTAGQGTNIGYRFEHLYEIIKKIDDKSRIGVCIDT
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
CHLFASGYDLRTKIDYESTFNKFFDLIEMKYLKGFHLNDSKKQFNSRVDRHENLGLGEIG
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
KSVFKWIIKNKNFHNIPMILETINPKLWEKEIDWLRSLQ
HHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYIGAHVSSSGGLEKAVFRAFQIKATTFSFFTKNQRQWISLPLQKKNIEDFKKACIKYN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHCCCCCEEECCCCCCCHHHHHHHHHCCC
FSPEKILPHSSYLINLGHPIDFFLEKSRVAFIDEIVRCDQLGLRFLNFHPGSHLNKISEV
CCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
TCLSRISESINIALEKTKNVVAVIENTAGQGTNIGYRFEHLYEIIKKIDDKSRIGVCIDT
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
CHLFASGYDLRTKIDYESTFNKFFDLIEMKYLKGFHLNDSKKQFNSRVDRHENLGLGEIG
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
KSVFKWIIKNKNFHNIPMILETINPKLWEKEIDWLRSLQ
HHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438