Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 21672411

GI number: 21672411

Start: 134240

End: 135229

Strand: Direct

Name: pheS

Synonym: BUsg121

Alternate gene names: 21672411

Gene position: 134240-135229 (Clockwise)

Preceding gene: 21672410

Following gene: 21672412

Centisome position: 20.93

GC content: 23.54

Gene sequence:

>990_bases
ATGTTGATTTTACAAAAATTATTTGACAGCGTAAAAATTGAAATTAAGAATTCACACACAATAGATAAATTAGATCATAT
ACGAATTAAATATTTAGGGAAAAAAGGAATTTTTTCTAATCTTATAAAAGATTTAAAAAATTTTTCTCTTGAAGAAAGAA
AAAAATACTTTATCATTTTTAATGAAATTAAAAAAAAAACAATAAATCAAATTAATAAAAAAAAAATAGAACTAAATAAA
ATTATTTTAGATAAACAGATTGAAAAAGAAAAAATTGATGTATCTTTACCTGGTCGTCGTATAAAAAACGGTGTTATTCA
TCCTATTAATCATACTATTACTTATATAAAAAATTTTTTTTCTAAATTAGGATTTGAATCTATTAGTGGATTTGAAATAG
AAGACGAATATCATAATTTTGATGCATTAAATATTCCTAAAGATCATCCTGCTCGCAATATTCATGATACCTTTTGGTTT
GATGAAAATCGTTTATTAAGAACACAAACTTCTAGTATGCAAATTAGGATTATGAAAAAAGAAAAACCTCCTATTAGGTT
GATTTTTCCTGGAAAAGTATATCGTAATGATTATGATTCTACACATACACCTATGTTTCATCAAGTTGAAGGATTAATAG
TCGAAAAAAATATTAATTTTTCTAATTTAAAATGGATTATATATAATTTTTTACGTAATTTTTTTCATAAAGATATTGCT
ATTAGATTTCGTCCATCTTATTTTCCGTTTACTACACCTTCTGCAGAAGTTGATGTTGTTACAAATAATGGAAAATTATT
AGAAGTATTAGGATGTGGTATGGTTCATCCATCAGTTTTAAAAAATGTGAATATTGATTCAAATTTTTATTCTGCTTGTG
CATTTGGAATTGGTATAGAACGAATTACTATGCTACGTTATGGAATTTCTGATCTTCGTTCTTTTTTTGAAAACGACATA
AGGTTTATAAAACAATTTAAACATATTTAG

Upstream 100 bases:

>100_bases
TTTAAATGTAAAACATACTAAGCTTCCTTTATGGAAGCTTTTTCATATTTTAAATATATGTATTTAAATTAAAAATATAA
AAAAAATAGGTATATAATAT

Downstream 100 bases:

>100_bases
TGAGATAAAATGAAATTTAATGAAAACTGGTTACGTGAATGGATTAATCCGAAAATAACTAGTGTTAGTTTAAGAAATCA
AATTGTTGAATCTGGTATAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLILQKLFDSVKIEIKNSHTIDKLDHIRIKYLGKKGIFSNLIKDLKNFSLEERKKYFIIFNEIKKKTINQINKKKIELNK
IILDKQIEKEKIDVSLPGRRIKNGVIHPINHTITYIKNFFSKLGFESISGFEIEDEYHNFDALNIPKDHPARNIHDTFWF
DENRLLRTQTSSMQIRIMKKEKPPIRLIFPGKVYRNDYDSTHTPMFHQVEGLIVEKNINFSNLKWIIYNFLRNFFHKDIA
IRFRPSYFPFTTPSAEVDVVTNNGKLLEVLGCGMVHPSVLKNVNIDSNFYSACAFGIGIERITMLRYGISDLRSFFENDI
RFIKQFKHI

Sequences:

>Translated_329_residues
MLILQKLFDSVKIEIKNSHTIDKLDHIRIKYLGKKGIFSNLIKDLKNFSLEERKKYFIIFNEIKKKTINQINKKKIELNK
IILDKQIEKEKIDVSLPGRRIKNGVIHPINHTITYIKNFFSKLGFESISGFEIEDEYHNFDALNIPKDHPARNIHDTFWF
DENRLLRTQTSSMQIRIMKKEKPPIRLIFPGKVYRNDYDSTHTPMFHQVEGLIVEKNINFSNLKWIIYNFLRNFFHKDIA
IRFRPSYFPFTTPSAEVDVVTNNGKLLEVLGCGMVHPSVLKNVNIDSNFYSACAFGIGIERITMLRYGISDLRSFFENDI
RFIKQFKHI
>Mature_329_residues
MLILQKLFDSVKIEIKNSHTIDKLDHIRIKYLGKKGIFSNLIKDLKNFSLEERKKYFIIFNEIKKKTINQINKKKIELNK
IILDKQIEKEKIDVSLPGRRIKNGVIHPINHTITYIKNFFSKLGFESISGFEIEDEYHNFDALNIPKDHPARNIHDTFWF
DENRLLRTQTSSMQIRIMKKEKPPIRLIFPGKVYRNDYDSTHTPMFHQVEGLIVEKNINFSNLKWIIYNFLRNFFHKDIA
IRFRPSYFPFTTPSAEVDVVTNNGKLLEVLGCGMVHPSVLKNVNIDSNFYSACAFGIGIERITMLRYGISDLRSFFENDI
RFIKQFKHI

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=29.5620437956204, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4758340, Length=268, Percent_Identity=30.5970149253731, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=59.9388379204893, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32566635, Length=280, Percent_Identity=32.8571428571429, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=29.4339622641509, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=264, Percent_Identity=29.1666666666667, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=27.9850746268657, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=37.7777777777778, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18858079, Length=266, Percent_Identity=27.8195488721804, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137424, Length=239, Percent_Identity=27.6150627615063, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BUCAP (P59056)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660478.1
- ProteinModelPortal:   P59056
- SMR:   P59056
- EnsemblBacteria:   EBBUCT00000000485
- GeneID:   1005938
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg121
- GeneTree:   EBGT00050000008141
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   BAPH198804:BUSG121-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38863; Mature: 38863

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILQKLFDSVKIEIKNSHTIDKLDHIRIKYLGKKGIFSNLIKDLKNFSLEERKKYFIIF
CCHHHHHHHHEEEEECCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCHHHCCEEEEEE
NEIKKKTINQINKKKIELNKIILDKQIEKEKIDVSLPGRRIKNGVIHPINHTITYIKNFF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCHHHHCCCCCCHHHHHHHHHHHH
SKLGFESISGFEIEDEYHNFDALNIPKDHPARNIHDTFWFDENRLLRTQTSSMQIRIMKK
HHCCCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEEEEC
EKPPIRLIFPGKVYRNDYDSTHTPMFHQVEGLIVEKNINFSNLKWIIYNFLRNFFHKDIA
CCCCEEEEECCHHCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCEE
IRFRPSYFPFTTPSAEVDVVTNNGKLLEVLGCGMVHPSVLKNVNIDSNFYSACAFGIGIE
EEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHCCHH
RITMLRYGISDLRSFFENDIRFIKQFKHI
HHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLILQKLFDSVKIEIKNSHTIDKLDHIRIKYLGKKGIFSNLIKDLKNFSLEERKKYFIIF
CCHHHHHHHHEEEEECCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCHHHCCEEEEEE
NEIKKKTINQINKKKIELNKIILDKQIEKEKIDVSLPGRRIKNGVIHPINHTITYIKNFF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCHHHHCCCCCCHHHHHHHHHHHH
SKLGFESISGFEIEDEYHNFDALNIPKDHPARNIHDTFWFDENRLLRTQTSSMQIRIMKK
HHCCCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEEEEC
EKPPIRLIFPGKVYRNDYDSTHTPMFHQVEGLIVEKNINFSNLKWIIYNFLRNFFHKDIA
CCCCEEEEECCHHCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCEE
IRFRPSYFPFTTPSAEVDVVTNNGKLLEVLGCGMVHPSVLKNVNIDSNFYSACAFGIGIE
EEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHCCHH
RITMLRYGISDLRSFFENDIRFIKQFKHI
HHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438