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The map label for this gene is pheT

Identifier: 21672412

GI number: 21672412

Start: 135239

End: 137635

Strand: Direct

Name: pheT

Synonym: BUsg122

Alternate gene names: 21672412

Gene position: 135239-137635 (Clockwise)

Preceding gene: 21672411

Following gene: 21672413

Centisome position: 21.08

GC content: 22.61

Gene sequence:

>2397_bases
ATGAAATTTAATGAAAACTGGTTACGTGAATGGATTAATCCGAAAATAACTAGTGTTAGTTTAAGAAATCAAATTGTTGA
ATCTGGTATAGAAATAGAATCAATACATGAATTTAATCCTATTTTTGATGGTTTTTTAGTTGGTAAAATAGTTGAATGCA
TCAATCCTTGTAAAGGAAATAATTTAAAAATATTAAAAGTAGATGTAGGATATAAAAAATTATTAAATATTGTATGTGGA
GCTTCAAACTGTCGTAATAATATTAAAGTTGTAGTGGCTACGATTGATAGTATTTTGCCAAATGGATCAAAAATTAAGAT
AAAAAAAATAAAAGAAAAATTATCTGAAGGTATGATATGTTCTTTTTTTGAATTAGGTTTGTTTAATTTTTGCAAAGATA
TTATTGAACTTCCTGAAGATATTCCTATAGGAAAAAAAATAAACGATTTATTTTTATTAAAAAAAGATACTTTTATTAAA
GTTGCTGTTACACCTAATCGTCCAGATGGATTAAGCATTTTAGGAATAGCTCGAAATATAGCTGCTATTAATAATTTAAA
AAAAATCCGATTAAAAAAAAGAATTCTTCCAACAACAATTGAAGATCAATTTCCTATTACAATTAATACTGAAAAACAAA
GTGTTAATTATTTCGGAAGAATTATTCAAAATGTTAATTTAAATGTTGACACTCCATTTTGGATGAAAAAAAAATTATTT
TTTTGTGATTTGTTATCAGATAATATAATTGAAAATATTCTTAATTATATTTTAATAGAAATTGGACAACCATTAAATAT
ATTAAATGCAGATAAAATAGATGATGTAATTGAAATTCGTATGGCTATAAAAAAAGAGTTTTTAATTTTAAAAAATGATA
CTCGAATAGTATTAGATAAAGATATTTTAGTGTTTTCAGATAAAACAAAAATATTGTTTATCCCGGGTAATATAAACAAT
TCTGATTTAGAACCAAATAAAAATACTAAAAATATATTTTTAACATCATATTTAGTTGATAAAAAATCGATATTAAATAT
TTTAAAAAAAATAGATTCTAATAATATTTTAGACTATTATAGTCATGGTGTTGATGCATCACTGCAAAAATATGCTATAG
AATATGCAACATATTTAATTGTTAAGATATGTGGTGGTAAAATAGGTCCTATTAATACAAAAAAATCTAATTTTAATTCT
TTATCTTGCTCAAATAAGATTAAGTTATATCATCAAAACTTCAATAAATGTATAGATTCTTTCGTTGATTCTTCTATTAT
TTCAAACATTTTATTATGTCTTGAATATAAAGTTAATTTTCACAAAAAATATTGGTATGTGTTTCCACCTAGTTGGCGAT
TTGATATTTTGATTGAAGAAGATGTAATAGGTGATATACTTCGTATATACAATTATAATAATATTCCTTTAACTCCATTA
AAACAAAATTACTATTTTAATAGTAAAAATAAAGACTTAAAAAGTCCCTTATTAGATGAAGCAGCTGTATTACTTATTAA
TAGAGGATATTATGAAATAATAACATATTCTTTTATCAATCCCTCTCTACAAGATGATATTATTCCAAATAATAATCAAA
TATTAATTTCTAATCCTATTTCTAAAGATTTTTCGTCTATGCGTTTATCATTATGGCCAGGTCTTCTTAAGACTGTTTCT
TATAACAAAAATCGTCAACAAGAAAGTATGCGTTTTTTCGAAAGAGGACTCTGTTTTTCAATTGATGAATCACAAATTCT
TGGAATACGACAAGAAATGTTTTTAGGAGGCGTAATAAGTGGTTTTTATAGTAAAGAAAACTGGTTTTCTGTTAGAAGGA
AAGTAGATTTTTATGATTTAAAAGGTGATTTGGAATCTTTATTAGAAGTTATATGCGGATTAAATAAATTTGAAATAAGG
CATCAAAATATATTAGGTTTACATCCAGAACAAAGTGCAAAGATTTATTTAGATAATAAGTATATTGGTAGTCTTGGTAA
AATTCATCCGAAAATAGAAAAAAAGTTAAATTTATATAATTCTACATTTTTATTTGAACTATCATTAAATTATATTTCAA
AATTAAAATTTTATAATACAGAAGAAATTTCTAAATATCCTACAAGTCGTCGTGATATAGCTATATTAGTATCCAAAGAT
ATTCCGTTTTTAGACATTATTACAGTATGTAAAGATTTTTTTGTTAATAAAAAAGTAGAAATTAATTTATTTGATGTATA
TTCTTGTAAAGAATTTGATAACAGAAAAAAAAGTTTAGGAATTAGTTTTGTATTTCAAAATTTTAAAAAAAATTTAAAAG
AAAATGAAGTTAACTTAATGCTTCATGATTGTATAAAAATATTAAAGAAAAAATTTCAAGTAGTTTTAAGGAAATAA

Upstream 100 bases:

>100_bases
ACGAATTACTATGCTACGTTATGGAATTTCTGATCTTCGTTCTTTTTTTGAAAACGACATAAGGTTTATAAAACAATTTA
AACATATTTAGTGAGATAAA

Downstream 100 bases:

>100_bases
ATTTATGGTAGTTACAAAAGCTGCAATTTCAGAAAATTTATTTGAAAAATTAAAATTAACTAAACGTGATTCTAAAGAAT
TTGTAGAGTTTTTTTTTGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MKFNENWLREWINPKITSVSLRNQIVESGIEIESIHEFNPIFDGFLVGKIVECINPCKGNNLKILKVDVGYKKLLNIVCG
ASNCRNNIKVVVATIDSILPNGSKIKIKKIKEKLSEGMICSFFELGLFNFCKDIIELPEDIPIGKKINDLFLLKKDTFIK
VAVTPNRPDGLSILGIARNIAAINNLKKIRLKKRILPTTIEDQFPITINTEKQSVNYFGRIIQNVNLNVDTPFWMKKKLF
FCDLLSDNIIENILNYILIEIGQPLNILNADKIDDVIEIRMAIKKEFLILKNDTRIVLDKDILVFSDKTKILFIPGNINN
SDLEPNKNTKNIFLTSYLVDKKSILNILKKIDSNNILDYYSHGVDASLQKYAIEYATYLIVKICGGKIGPINTKKSNFNS
LSCSNKIKLYHQNFNKCIDSFVDSSIISNILLCLEYKVNFHKKYWYVFPPSWRFDILIEEDVIGDILRIYNYNNIPLTPL
KQNYYFNSKNKDLKSPLLDEAAVLLINRGYYEIITYSFINPSLQDDIIPNNNQILISNPISKDFSSMRLSLWPGLLKTVS
YNKNRQQESMRFFERGLCFSIDESQILGIRQEMFLGGVISGFYSKENWFSVRRKVDFYDLKGDLESLLEVICGLNKFEIR
HQNILGLHPEQSAKIYLDNKYIGSLGKIHPKIEKKLNLYNSTFLFELSLNYISKLKFYNTEEISKYPTSRRDIAILVSKD
IPFLDIITVCKDFFVNKKVEINLFDVYSCKEFDNRKKSLGISFVFQNFKKNLKENEVNLMLHDCIKILKKKFQVVLRK

Sequences:

>Translated_798_residues
MKFNENWLREWINPKITSVSLRNQIVESGIEIESIHEFNPIFDGFLVGKIVECINPCKGNNLKILKVDVGYKKLLNIVCG
ASNCRNNIKVVVATIDSILPNGSKIKIKKIKEKLSEGMICSFFELGLFNFCKDIIELPEDIPIGKKINDLFLLKKDTFIK
VAVTPNRPDGLSILGIARNIAAINNLKKIRLKKRILPTTIEDQFPITINTEKQSVNYFGRIIQNVNLNVDTPFWMKKKLF
FCDLLSDNIIENILNYILIEIGQPLNILNADKIDDVIEIRMAIKKEFLILKNDTRIVLDKDILVFSDKTKILFIPGNINN
SDLEPNKNTKNIFLTSYLVDKKSILNILKKIDSNNILDYYSHGVDASLQKYAIEYATYLIVKICGGKIGPINTKKSNFNS
LSCSNKIKLYHQNFNKCIDSFVDSSIISNILLCLEYKVNFHKKYWYVFPPSWRFDILIEEDVIGDILRIYNYNNIPLTPL
KQNYYFNSKNKDLKSPLLDEAAVLLINRGYYEIITYSFINPSLQDDIIPNNNQILISNPISKDFSSMRLSLWPGLLKTVS
YNKNRQQESMRFFERGLCFSIDESQILGIRQEMFLGGVISGFYSKENWFSVRRKVDFYDLKGDLESLLEVICGLNKFEIR
HQNILGLHPEQSAKIYLDNKYIGSLGKIHPKIEKKLNLYNSTFLFELSLNYISKLKFYNTEEISKYPTSRRDIAILVSKD
IPFLDIITVCKDFFVNKKVEINLFDVYSCKEFDNRKKSLGISFVFQNFKKNLKENEVNLMLHDCIKILKKKFQVVLRK
>Mature_798_residues
MKFNENWLREWINPKITSVSLRNQIVESGIEIESIHEFNPIFDGFLVGKIVECINPCKGNNLKILKVDVGYKKLLNIVCG
ASNCRNNIKVVVATIDSILPNGSKIKIKKIKEKLSEGMICSFFELGLFNFCKDIIELPEDIPIGKKINDLFLLKKDTFIK
VAVTPNRPDGLSILGIARNIAAINNLKKIRLKKRILPTTIEDQFPITINTEKQSVNYFGRIIQNVNLNVDTPFWMKKKLF
FCDLLSDNIIENILNYILIEIGQPLNILNADKIDDVIEIRMAIKKEFLILKNDTRIVLDKDILVFSDKTKILFIPGNINN
SDLEPNKNTKNIFLTSYLVDKKSILNILKKIDSNNILDYYSHGVDASLQKYAIEYATYLIVKICGGKIGPINTKKSNFNS
LSCSNKIKLYHQNFNKCIDSFVDSSIISNILLCLEYKVNFHKKYWYVFPPSWRFDILIEEDVIGDILRIYNYNNIPLTPL
KQNYYFNSKNKDLKSPLLDEAAVLLINRGYYEIITYSFINPSLQDDIIPNNNQILISNPISKDFSSMRLSLWPGLLKTVS
YNKNRQQESMRFFERGLCFSIDESQILGIRQEMFLGGVISGFYSKENWFSVRRKVDFYDLKGDLESLLEVICGLNKFEIR
HQNILGLHPEQSAKIYLDNKYIGSLGKIHPKIEKKLNLYNSTFLFELSLNYISKLKFYNTEEISKYPTSRRDIAILVSKD
IPFLDIITVCKDFFVNKKVEINLFDVYSCKEFDNRKKSLGISFVFQNFKKNLKENEVNLMLHDCIKILKKKFQVVLRK

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=799, Percent_Identity=40.5506883604506, Blast_Score=619, Evalue=1e-178,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_BUCAP (P59057)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660479.1
- ProteinModelPortal:   P59057
- EnsemblBacteria:   EBBUCT00000000260
- GeneID:   1005939
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg122
- GeneTree:   EBGT00050000007758
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   BAPH198804:BUSG122-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 92419; Mature: 92419

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNENWLREWINPKITSVSLRNQIVESGIEIESIHEFNPIFDGFLVGKIVECINPCKGN
CCCCHHHHHHHCCCCEEEEHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCC
NLKILKVDVGYKKLLNIVCGASNCRNNIKVVVATIDSILPNGSKIKIKKIKEKLSEGMIC
CEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEEHHHHHHHHHCCCCH
SFFELGLFNFCKDIIELPEDIPIGKKINDLFLLKKDTFIKVAVTPNRPDGLSILGIARNI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEHHHHHH
AAINNLKKIRLKKRILPTTIEDQFPITINTEKQSVNYFGRIIQNVNLNVDTPFWMKKKLF
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FCDLLSDNIIENILNYILIEIGQPLNILNADKIDDVIEIRMAIKKEFLILKNDTRIVLDK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCEEEEEC
DILVFSDKTKILFIPGNINNSDLEPNKNTKNIFLTSYLVDKKSILNILKKIDSNNILDYY
CEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHCHHHHHHHHHHCCCCCCHHHH
SHGVDASLQKYAIEYATYLIVKICGGKIGPINTKKSNFNSLSCSNKIKLYHQNFNKCIDS
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FVDSSIISNILLCLEYKVNFHKKYWYVFPPSWRFDILIEEDVIGDILRIYNYNNIPLTPL
HHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHCCCCCCCCCC
KQNYYFNSKNKDLKSPLLDEAAVLLINRGYYEIITYSFINPSLQDDIIPNNNQILISNPI
CCCCEECCCCCCCCCCHHHCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCC
SKDFSSMRLSLWPGLLKTVSYNKNRQQESMRFFERGLCFSIDESQILGIRQEMFLGGVIS
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
GFYSKENWFSVRRKVDFYDLKGDLESLLEVICGLNKFEIRHQNILGLHPEQSAKIYLDNK
HHCCCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCEEEECCH
YIGSLGKIHPKIEKKLNLYNSTFLFELSLNYISKLKFYNTEEISKYPTSRRDIAILVSKD
HHCCHHHCCHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECC
IPFLDIITVCKDFFVNKKVEINLFDVYSCKEFDNRKKSLGISFVFQNFKKNLKENEVNLM
CCHHHHHHHHHHHHCCCEEEEEEEEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LHDCIKILKKKFQVVLRK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFNENWLREWINPKITSVSLRNQIVESGIEIESIHEFNPIFDGFLVGKIVECINPCKGN
CCCCHHHHHHHCCCCEEEEHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCC
NLKILKVDVGYKKLLNIVCGASNCRNNIKVVVATIDSILPNGSKIKIKKIKEKLSEGMIC
CEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEEHHHHHHHHHCCCCH
SFFELGLFNFCKDIIELPEDIPIGKKINDLFLLKKDTFIKVAVTPNRPDGLSILGIARNI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCEEEHHHHHH
AAINNLKKIRLKKRILPTTIEDQFPITINTEKQSVNYFGRIIQNVNLNVDTPFWMKKKLF
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FCDLLSDNIIENILNYILIEIGQPLNILNADKIDDVIEIRMAIKKEFLILKNDTRIVLDK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCEEEEEC
DILVFSDKTKILFIPGNINNSDLEPNKNTKNIFLTSYLVDKKSILNILKKIDSNNILDYY
CEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHCHHHHHHHHHHCCCCCCHHHH
SHGVDASLQKYAIEYATYLIVKICGGKIGPINTKKSNFNSLSCSNKIKLYHQNFNKCIDS
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FVDSSIISNILLCLEYKVNFHKKYWYVFPPSWRFDILIEEDVIGDILRIYNYNNIPLTPL
HHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHCCCCCCCCCC
KQNYYFNSKNKDLKSPLLDEAAVLLINRGYYEIITYSFINPSLQDDIIPNNNQILISNPI
CCCCEECCCCCCCCCCHHHCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCC
SKDFSSMRLSLWPGLLKTVSYNKNRQQESMRFFERGLCFSIDESQILGIRQEMFLGGVIS
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
GFYSKENWFSVRRKVDFYDLKGDLESLLEVICGLNKFEIRHQNILGLHPEQSAKIYLDNK
HHCCCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCEEEECCH
YIGSLGKIHPKIEKKLNLYNSTFLFELSLNYISKLKFYNTEEISKYPTSRRDIAILVSKD
HHCCHHHCCHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECC
IPFLDIITVCKDFFVNKKVEINLFDVYSCKEFDNRKKSLGISFVFQNFKKNLKENEVNLM
CCHHHHHHHHHHHHCCCEEEEEEEEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LHDCIKILKKKFQVVLRK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438