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The map label for this gene is thrS

Identifier: 21672408

GI number: 21672408

Start: 130947

End: 132875

Strand: Direct

Name: thrS

Synonym: BUsg117

Alternate gene names: 21672408

Gene position: 130947-132875 (Clockwise)

Preceding gene: 21672407

Following gene: 30410815

Centisome position: 20.41

GC content: 25.66

Gene sequence:

>1929_bases
ATGCCTGTGATAAGATTTTGTGATGGGAGTCAGCAGGTATATAAGCATTCTGTCTCATTGAGAGAAATTATAGAAAATAA
AAAACCTAATATAATTAGGTCCCTTATTGCTATTTCTGTCAATAATAGTTTTTCAAATTTTAATACTTTAATAACAGAAG
ATTCTTCTATATCATTTATTAGTAAAAAAGATTGCGAAGCATTAAATATTATTCGATATTCTTGTATACAGCTTTTAAAT
TATGCAGCGAAAAAAACATGGCCATCGTGTAAGATTGGAGAAAGTGAAATTACAAAAAGTGGTTTTTATTGTGATATTGA
TTTTGAAAATAGTATTACAGAAGAAGATTTTTTTATATTAGAAAATAATATGAAAACTCTTATAAAAAGAGAATATTTTA
TATCTCATCAAAACATTTCTTTTGATCATGCGTATGAAATGTTTAAAAAGAAATCAGAAATATATAAGATTCATTTAATA
AAAAAATATATAAATAAAAAAAATAAAATATCATTGTATTATCATGAAAATTATTTTGATATTGATATGGGGATGCAAGT
TTTTAATATAAAATTTTGTAAATATTTTAAATTACAAAAAATTGGAGGTATTTATTGGAAAGGTGATCATAAAAATAAAA
TGTTACAACGTATTTACGGTACTGCTTGGTCAACTAAAAAAGAATTAGATAAACATTTAAGCTATATTAATGAATTAAAA
AAAAGAGATCATAGAAAAATTGGAAAATTGTTAAATTTATATCATATGCAGAAAGAATCTCCAGGTATGATATTTTGGCA
TAATAACGGTTGGATCATTTTTAATGAACTAGAAATATTTGTTCGAGAAAAACTAAAAGAATATAAATATAAGGAAGTTA
AAACACCTTTATTAATAGATAAATCAATATGGCAAAAAAGTGGTCATTGGGACAATTATCAAGATGCTATTTTTACTACT
TCATCAGAAAATCGAGAATATTGTATTAAACCAATGAATTGTCCAGGACATGTTCAAATTTTTAACTGTGGATTAAAGTC
ATATCGAGATCTACCTATTCGTATGGCTGAGTTTGGAAGTTGTCATCGTAACGAATCTTCAGGATCTTTGCATGGTCTTA
TGAGAATTCGTAATTTTACTCAAGATGATGCTCATATATTTTGTACCCAAGAGCAGTTGCGATATGAAATAAATAATTGT
ATTAAGATGATATATGATTTATATAGTACATTTAATTTTAAAAAAATATTAGTAAAATTCTCTACTCGACCAAAAAAACG
TATTGGAGATGAATCTGTTTGGGATCAAGCAGAAAAAGATTTATCTGATGTATTGATAGAAAATAATCTAAAGTTTGAAC
ATCAAGAAGGAGAAGGTGCTTTTTATGGTCCTAAAATTGAGTTTGTTTTACAGGACTCTTTAGATCGTAATTGGCAGTGT
GGAACGATTCAACTTGATTTTTATTTGCCAATTCGTTTACGTTCATTTTATATTGATGAACACAATCATCAAAAAATTCC
TATAATAATTCATCGAGCGATATTAGGTTCAATAGAACGTTTTATTGGAATATTAATTGAAGAGTTTTCAGGAAAATTGC
CTACATGGTTATCTCCAATACAGGTAGTAATACTCAGTATTACTGATAGTCATATAAATTACGTTAAAAAAATAGTCCAA
CATTTTTCTGATATTAATATTCGAGTTGAATCAGATTTAAGAAATGAAAAAATAGGTTTTAAAATTCGTGAACATACATT
GCGTCAAATTCCATATATATTAATTTGTGGAGAAAAAGAAATTAAATCTAAAAAAATTTCGGTTAGAACTAGAAATGGTT
ACAATTTAGGAATTATAGATATTGATTGTTTTATTAAAAAGTTACAAAAAGAAATTTTTACTCGTAGCTTTTATCAAATG
GAGGAATAA

Upstream 100 bases:

>100_bases
AGTCAAAAATTTGACTAGCATCTTTTTATTAAACTCAACAGTTGAAATTTTTTAAATAATTTTTAAAAAATAATATTTTT
TTAATTAAGGATCTAAAAGA

Downstream 100 bases:

>100_bases
AGTATTAAAGGCGGAAAAAGAATTCAATTCACGCGGCCGAATCGTATTAATAATGAAATTCGTGCCATTAAAGTTCGTCT
TACTGGTGTCGAAGGCGATC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MPVIRFCDGSQQVYKHSVSLREIIENKKPNIIRSLIAISVNNSFSNFNTLITEDSSISFISKKDCEALNIIRYSCIQLLN
YAAKKTWPSCKIGESEITKSGFYCDIDFENSITEEDFFILENNMKTLIKREYFISHQNISFDHAYEMFKKKSEIYKIHLI
KKYINKKNKISLYYHENYFDIDMGMQVFNIKFCKYFKLQKIGGIYWKGDHKNKMLQRIYGTAWSTKKELDKHLSYINELK
KRDHRKIGKLLNLYHMQKESPGMIFWHNNGWIIFNELEIFVREKLKEYKYKEVKTPLLIDKSIWQKSGHWDNYQDAIFTT
SSENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGSCHRNESSGSLHGLMRIRNFTQDDAHIFCTQEQLRYEINNC
IKMIYDLYSTFNFKKILVKFSTRPKKRIGDESVWDQAEKDLSDVLIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQC
GTIQLDFYLPIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFSGKLPTWLSPIQVVILSITDSHINYVKKIVQ
HFSDINIRVESDLRNEKIGFKIREHTLRQIPYILICGEKEIKSKKISVRTRNGYNLGIIDIDCFIKKLQKEIFTRSFYQM
EE

Sequences:

>Translated_642_residues
MPVIRFCDGSQQVYKHSVSLREIIENKKPNIIRSLIAISVNNSFSNFNTLITEDSSISFISKKDCEALNIIRYSCIQLLN
YAAKKTWPSCKIGESEITKSGFYCDIDFENSITEEDFFILENNMKTLIKREYFISHQNISFDHAYEMFKKKSEIYKIHLI
KKYINKKNKISLYYHENYFDIDMGMQVFNIKFCKYFKLQKIGGIYWKGDHKNKMLQRIYGTAWSTKKELDKHLSYINELK
KRDHRKIGKLLNLYHMQKESPGMIFWHNNGWIIFNELEIFVREKLKEYKYKEVKTPLLIDKSIWQKSGHWDNYQDAIFTT
SSENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGSCHRNESSGSLHGLMRIRNFTQDDAHIFCTQEQLRYEINNC
IKMIYDLYSTFNFKKILVKFSTRPKKRIGDESVWDQAEKDLSDVLIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQC
GTIQLDFYLPIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFSGKLPTWLSPIQVVILSITDSHINYVKKIVQ
HFSDINIRVESDLRNEKIGFKIREHTLRQIPYILICGEKEIKSKKISVRTRNGYNLGIIDIDCFIKKLQKEIFTRSFYQM
EE
>Mature_641_residues
PVIRFCDGSQQVYKHSVSLREIIENKKPNIIRSLIAISVNNSFSNFNTLITEDSSISFISKKDCEALNIIRYSCIQLLNY
AAKKTWPSCKIGESEITKSGFYCDIDFENSITEEDFFILENNMKTLIKREYFISHQNISFDHAYEMFKKKSEIYKIHLIK
KYINKKNKISLYYHENYFDIDMGMQVFNIKFCKYFKLQKIGGIYWKGDHKNKMLQRIYGTAWSTKKELDKHLSYINELKK
RDHRKIGKLLNLYHMQKESPGMIFWHNNGWIIFNELEIFVREKLKEYKYKEVKTPLLIDKSIWQKSGHWDNYQDAIFTTS
SENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGSCHRNESSGSLHGLMRIRNFTQDDAHIFCTQEQLRYEINNCI
KMIYDLYSTFNFKKILVKFSTRPKKRIGDESVWDQAEKDLSDVLIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQCG
TIQLDFYLPIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFSGKLPTWLSPIQVVILSITDSHINYVKKIVQH
FSDINIRVESDLRNEKIGFKIREHTLRQIPYILICGEKEIKSKKISVRTRNGYNLGIIDIDCFIKKLQKEIFTRSFYQME
E

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=604, Percent_Identity=36.5894039735099, Blast_Score=391, Evalue=1e-108,
Organism=Homo sapiens, GI38570111, Length=641, Percent_Identity=34.3213728549142, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI20070344, Length=619, Percent_Identity=31.6639741518578, Blast_Score=342, Evalue=6e-94,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=58.5669781931464, Blast_Score=835, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=614, Percent_Identity=33.7133550488599, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984184, Length=614, Percent_Identity=33.7133550488599, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6322112, Length=508, Percent_Identity=37.992125984252, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322654, Length=391, Percent_Identity=40.4092071611253, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24583839, Length=593, Percent_Identity=34.9072512647555, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=33.4920634920635, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=33.4920634920635, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_BUCAP (P46244)

Other databases:

- EMBL:   U11066
- EMBL:   AE013218
- PIR:   I40071
- RefSeq:   NP_660475.1
- ProteinModelPortal:   P46244
- SMR:   P46244
- EnsemblBacteria:   EBBUCT00000000177
- GeneID:   1005934
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg117
- GeneTree:   EBGT00050000007910
- HOGENOM:   HBG352811
- OMA:   GFEYEVE
- ProtClustDB:   PRK12305
- BioCyc:   BAPH198804:BUSG117-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 76228; Mature: 76097

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIRFCDGSQQVYKHSVSLREIIENKKPNIIRSLIAISVNNSFSNFNTLITEDSSISFI
CCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCCCEEEECCCCEEEE
SKKDCEALNIIRYSCIQLLNYAAKKTWPSCKIGESEITKSGFYCDIDFENSITEEDFFIL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCEEEE
ENNMKTLIKREYFISHQNISFDHAYEMFKKKSEIYKIHLIKKYINKKNKISLYYHENYFD
ECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEHEEHHHHHCCCCCEEEEEECCEEE
IDMGMQVFNIKFCKYFKLQKIGGIYWKGDHKNKMLQRIYGTAWSTKKELDKHLSYINELK
ECCCCEEEEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKLLNLYHMQKESPGMIFWHNNGWIIFNELEIFVREKLKEYKYKEVKTPLLID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEE
KSIWQKSGHWDNYQDAIFTTSSENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGS
HHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEECCHHHHCCCCEEHHHCCC
CHRNESSGSLHGLMRIRNFTQDDAHIFCTQEQLRYEINNCIKMIYDLYSTFNFKKILVKF
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPKKRIGDESVWDQAEKDLSDVLIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQC
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEECCCCCCCCC
GTIQLDFYLPIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFSGKLPTWLSPI
EEEEEEEEEEEEEEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCE
QVVILSITDSHINYVKKIVQHFSDINIRVESDLRNEKIGFKIREHTLRQIPYILICGEKE
EEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEHHHHHHHCCEEEEECCHH
IKSKKISVRTRNGYNLGIIDIDCFIKKLQKEIFTRSFYQMEE
HCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVIRFCDGSQQVYKHSVSLREIIENKKPNIIRSLIAISVNNSFSNFNTLITEDSSISFI
CEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCCCEEEECCCCEEEE
SKKDCEALNIIRYSCIQLLNYAAKKTWPSCKIGESEITKSGFYCDIDFENSITEEDFFIL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCEEEE
ENNMKTLIKREYFISHQNISFDHAYEMFKKKSEIYKIHLIKKYINKKNKISLYYHENYFD
ECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEHEEHHHHHCCCCCEEEEEECCEEE
IDMGMQVFNIKFCKYFKLQKIGGIYWKGDHKNKMLQRIYGTAWSTKKELDKHLSYINELK
ECCCCEEEEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKLLNLYHMQKESPGMIFWHNNGWIIFNELEIFVREKLKEYKYKEVKTPLLID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEE
KSIWQKSGHWDNYQDAIFTTSSENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGS
HHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEECCHHHHCCCCEEHHHCCC
CHRNESSGSLHGLMRIRNFTQDDAHIFCTQEQLRYEINNCIKMIYDLYSTFNFKKILVKF
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPKKRIGDESVWDQAEKDLSDVLIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQC
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEECCCCCCCCC
GTIQLDFYLPIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFSGKLPTWLSPI
EEEEEEEEEEEEEEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCE
QVVILSITDSHINYVKKIVQHFSDINIRVESDLRNEKIGFKIREHTLRQIPYILICGEKE
EEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEHHHHHHHCCEEEEECCHH
IKSKKISVRTRNGYNLGIIDIDCFIKKLQKEIFTRSFYQMEE
HCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766160; 12089438