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The map label for this gene is ribE

Identifier: 21672396

GI number: 21672396

Start: 117605

End: 118231

Strand: Direct

Name: ribE

Synonym: BUsg104

Alternate gene names: 21672396

Gene position: 117605-118231 (Clockwise)

Preceding gene: 21672394

Following gene: 21672397

Centisome position: 18.33

GC content: 24.24

Gene sequence:

>627_bases
ATGTTCACAGGCATTGTAAATGGCATTGCAAGAGTTGTTTCCATAAATAAGAAAAAAAATTTTCATACCTATACTGTTAA
TTTTTCGTCTATTTTGTTAAAAAATCTGAAAATAGGTGATTCAGTTGCTCATAATGGATGTTGTTTAACTGTTAAATATA
TTAATTGTCCTCATGTAGTTTTTGATATTATGAAAATTACGATTGCGAATACTAATTTAGGAGTTTTAAATATAGGAGAC
TATGTTAATATTGAAAGATCATTAAAATATGGTGATGAAATGGGAGGTCATATTATATCAGGTCATATTATGAATACAGG
TGAAATTTCTAAAATATCAAAATTAGATAAAAATTATATTCTATGGTGTAAAGTAAAAGATCTTTCTTTAATGAAATATA
TTTTTTATAAAGGTTTTATCGCTATTGATGGCATCAGTTTAACTATTAATAATATCATTAAAAATGAATTTTGCGTTAGT
ATAATACCAGAAACTTTATCTTCAACTACTATAGGATTTAAAAAAATAGGACAATTAGTGAATATTGAAATCGATTTTTA
TACTCAAATCATTGTAGATACAACAAAACGTTTGATTAAAAAAGATATATCTACGTTGTTTAAGTAG

Upstream 100 bases:

>100_bases
ATTATAATATACAAATATTTTTTATAAAAAATCAGATATTTTCTAAATTTTATTAACTTTAAATTTATTATCTACATTGT
ATTAATTTTAAGAGGGTTTT

Downstream 100 bases:

>100_bases
TTTCATTTTTTTAGTCATTTCAAGGAAATTATCAATGAAAAGTTACTTTTTTTTTATTTTATCAAATATATTAATTGATA
ATTTTATTTTAGTAAAATTT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MFTGIVNGIARVVSINKKKNFHTYTVNFSSILLKNLKIGDSVAHNGCCLTVKYINCPHVVFDIMKITIANTNLGVLNIGD
YVNIERSLKYGDEMGGHIISGHIMNTGEISKISKLDKNYILWCKVKDLSLMKYIFYKGFIAIDGISLTINNIIKNEFCVS
IIPETLSSTTIGFKKIGQLVNIEIDFYTQIIVDTTKRLIKKDISTLFK

Sequences:

>Translated_208_residues
MFTGIVNGIARVVSINKKKNFHTYTVNFSSILLKNLKIGDSVAHNGCCLTVKYINCPHVVFDIMKITIANTNLGVLNIGD
YVNIERSLKYGDEMGGHIISGHIMNTGEISKISKLDKNYILWCKVKDLSLMKYIFYKGFIAIDGISLTINNIIKNEFCVS
IIPETLSSTTIGFKKIGQLVNIEIDFYTQIIVDTTKRLIKKDISTLFK
>Mature_208_residues
MFTGIVNGIARVVSINKKKNFHTYTVNFSSILLKNLKIGDSVAHNGCCLTVKYINCPHVVFDIMKITIANTNLGVLNIGD
YVNIERSLKYGDEMGGHIISGHIMNTGEISKISKLDKNYILWCKVKDLSLMKYIFYKGFIAIDGISLTINNIIKNEFCVS
IIPETLSSTTIGFKKIGQLVNIEIDFYTQIIVDTTKRLIKKDISTLFK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats

Homologues:

Organism=Escherichia coli, GI1787952, Length=199, Percent_Identity=56.78391959799, Blast_Score=240, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6319733, Length=221, Percent_Identity=33.0316742081448, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISA_BUCAP (Q8KA22)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660463.1
- ProteinModelPortal:   Q8KA22
- SMR:   Q8KA22
- EnsemblBacteria:   EBBUCT00000000283
- GeneID:   1005921
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg104
- GeneTree:   EBGT00050000007992
- HOGENOM:   HBG289809
- OMA:   CCLTVTK
- ProtClustDB:   PRK09289
- BioCyc:   BAPH198804:BUSG104-MONOMER
- InterPro:   IPR001783
- InterPro:   IPR017938
- PANTHER:   PTHR21098
- PIRSF:   PIRSF000498
- TIGRFAMs:   TIGR00187

Pfam domain/function: PF00677 Lum_binding; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =2.5.1.9

Molecular weight: Translated: 23365; Mature: 23365

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS51177 LUMAZINE_BIND; PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVNGIARVVSINKKKNFHTYTVNFSSILLKNLKIGDSVAHNGCCLTVKYINCPHVV
CCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHH
FDIMKITIANTNLGVLNIGDYVNIERSLKYGDEMGGHIISGHIMNTGEISKISKLDKNYI
HHHHHEEEECCCCCEEECCCEEEHHHHHCCCHHCCCEEEEEEEECCCCHHHHHHCCCCEE
LWCKVKDLSLMKYIFYKGFIAIDGISLTINNIIKNEFCVSIIPETLSSTTIGFKKIGQLV
EEEEECCHHHHHHHHHCCEEEECCEEEEEHHHHCCCHHEEECHHHHCCCCCCHHHCCCEE
NIEIDFYTQIIVDTTKRLIKKDISTLFK
EEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTGIVNGIARVVSINKKKNFHTYTVNFSSILLKNLKIGDSVAHNGCCLTVKYINCPHVV
CCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHH
FDIMKITIANTNLGVLNIGDYVNIERSLKYGDEMGGHIISGHIMNTGEISKISKLDKNYI
HHHHHEEEECCCCCEEECCCEEEHHHHHCCCHHCCCEEEEEEEECCCCHHHHHHCCCCEE
LWCKVKDLSLMKYIFYKGFIAIDGISLTINNIIKNEFCVSIIPETLSSTTIGFKKIGQLV
EEEEECCHHHHHHHHHCCEEEECCEEEEEHHHHCCCHHEEECHHHHCCCCCCHHHCCCEE
NIEIDFYTQIIVDTTKRLIKKDISTLFK
EEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438