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The map label for this gene is rnfA

Identifier: 21672397

GI number: 21672397

Start: 118266

End: 118847

Strand: Direct

Name: rnfA

Synonym: BUsg105

Alternate gene names: 21672397

Gene position: 118266-118847 (Clockwise)

Preceding gene: 21672396

Following gene: 21672398

Centisome position: 18.44

GC content: 26.63

Gene sequence:

>582_bases
ATGAAAAGTTACTTTTTTTTTATTTTATCAAATATATTAATTGATAATTTTATTTTAGTAAAATTTCTTGGTTTATGTCC
CTTCATAGGTGCGTCAAATCAAATAAAACAAGCTTTTGGAATAAGTTTCGCCACTTCTTTTGTTGTAGTTGTATCGACAG
TTTTATTATGGTTTGTTAACTTTTTTATTTTGTTACCTTTTGATTTAGTTTATTTAAGAATTATAGTGTATATGTTAATT
ATTTCTTTTGGTGTTCAATTAATAGAAATTATTTTACGAGGGACTAGTCCTATCTTATATCGTATACTTGGTATTTTTCT
CCCTTTAATTACAACTAATTGTGCCGTTTTAGCTATTCCATTGTTTAGTCTCTATTTAAATCATACATTCTTAGAATCTA
TTCTTTATGCAGTCAGTGCCTCTTTTGGTTTTACTTTAGTGATGGTTATATTTTCTAGTATTCGTGAACGTATATTATTA
TCTGACGTTCCTTTAGCATTTCAAGGGTCGCCTATTGTTCTTATTACTGTAAGTTTAATATCTATTGTATTTATGGGGTT
TAAAGGTTTAGTGAGAATTTAA

Upstream 100 bases:

>100_bases
ACTCAAATCATTGTAGATACAACAAAACGTTTGATTAAAAAAGATATATCTACGTTGTTTAAGTAGTTTCATTTTTTTAG
TCATTTCAAGGAAATTATCA

Downstream 100 bases:

>100_bases
TTATGTCTATTACAATTATTTTAATTTTTTCTATCTTATCTTTTCTTTTAGGTATAATATTAAGTTATGTAAAATATATT
TTTCCAATAAAAAAAAATTC

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKSYFFFILSNILIDNFILVKFLGLCPFIGASNQIKQAFGISFATSFVVVVSTVLLWFVNFFILLPFDLVYLRIIVYMLI
ISFGVQLIEIILRGTSPILYRILGIFLPLITTNCAVLAIPLFSLYLNHTFLESILYAVSASFGFTLVMVIFSSIRERILL
SDVPLAFQGSPIVLITVSLISIVFMGFKGLVRI

Sequences:

>Translated_193_residues
MKSYFFFILSNILIDNFILVKFLGLCPFIGASNQIKQAFGISFATSFVVVVSTVLLWFVNFFILLPFDLVYLRIIVYMLI
ISFGVQLIEIILRGTSPILYRILGIFLPLITTNCAVLAIPLFSLYLNHTFLESILYAVSASFGFTLVMVIFSSIRERILL
SDVPLAFQGSPIVLITVSLISIVFMGFKGLVRI
>Mature_193_residues
MKSYFFFILSNILIDNFILVKFLGLCPFIGASNQIKQAFGISFATSFVVVVSTVLLWFVNFFILLPFDLVYLRIIVYMLI
ISFGVQLIEIILRGTSPILYRILGIFLPLITTNCAVLAIPLFSLYLNHTFLESILYAVSASFGFTLVMVIFSSIRERILL
SDVPLAFQGSPIVLITVSLISIVFMGFKGLVRI

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=50.259067357513, Blast_Score=217, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_BUCAP (Q8KA21)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660464.1
- EnsemblBacteria:   EBBUCT00000000521
- GeneID:   1005922
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg105
- GeneTree:   EBGT00050000007905
- HOGENOM:   HBG624915
- OMA:   LRTIAFI
- ProtClustDB:   PRK05151
- BioCyc:   BAPH198804:BUSG105-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 21685; Mature: 21685

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb2876d8)-; HASH(0x97084c0)-; HASH(0x96ec880)-; HASH(0xbc0aa04)-; HASH(0xba657bc)-; HASH(0x964a528)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYFFFILSNILIDNFILVKFLGLCPFIGASNQIKQAFGISFATSFVVVVSTVLLWFVN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFILLPFDLVYLRIIVYMLIISFGVQLIEIILRGTSPILYRILGIFLPLITTNCAVLAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFSLYLNHTFLESILYAVSASFGFTLVMVIFSSIRERILLSDVPLAFQGSPIVLITVSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SIVFMGFKGLVRI
HHHHHHHHHHHCH
>Mature Secondary Structure
MKSYFFFILSNILIDNFILVKFLGLCPFIGASNQIKQAFGISFATSFVVVVSTVLLWFVN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFILLPFDLVYLRIIVYMLIISFGVQLIEIILRGTSPILYRILGIFLPLITTNCAVLAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFSLYLNHTFLESILYAVSASFGFTLVMVIFSSIRERILLSDVPLAFQGSPIVLITVSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SIVFMGFKGLVRI
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438