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The map label for this gene is hisA

Identifier: 21672389

GI number: 21672389

Start: 109558

End: 110292

Strand: Direct

Name: hisA

Synonym: BUsg097

Alternate gene names: 21672389

Gene position: 109558-110292 (Clockwise)

Preceding gene: 21672388

Following gene: 21672390

Centisome position: 17.08

GC content: 25.85

Gene sequence:

>735_bases
ATGATTATTCCAGCATTTGATTTAATTAATGGCGAAATAGTTCGCTTGTATAAAGGAAATTTTTTTAATCGAACAAAATA
TGACATAAATTTATATAAACGTTTGAAAGATTATGAAATTAAAGGAGTAAAAAAAATTCATTTGGTAGATTTAGATGGTG
CAAAAAATATTAAAGACAGACAAATAAAAGTTTTTAGGGATATTATTTCTTATACAAATATTCCTATACAAATAGGTGGT
GGAATAAGAAACGAAGAAGACATTAATATGTTTCTTAATTTAGGAGTTAAGAAAGTTGTAATTGGTTCTTCTGCAATAAA
AAACAAAACAAAAGTGAAAAAATGGCTAAAAATATATGGCCCCAATGTTATTATTTTGGCACTAGATATTATTATTAAAA
ATAATAATAAAGAGATAGCAATACATGGTTGGCAGGAGACAACGAATATAACTTTAGAAGAAATTATTGAGTATTTTTCA
CCAATTGGTTTAAAACATGTTTTATGTACTGATATATCGAAAGATGGAACGTTATCTGGTCCTAATAATATATTATATAA
AGATATTGTGAAAAAATTTAAACATATATCGTTTCAAGCGTCAGGAGGAGTTTCAAGATTAAAAGATCTTCTATCTTTAA
AAAAAACAGGTGTTAAAAGTGTTATTATTGGTCGTAGTTTGTTAGAAAATAAGTTTACAATAGAAGAGGCATTAAAATGC
TGGCAAAGCGAATAA

Upstream 100 bases:

>100_bases
AATGCAAAAAAATAATTTTTTCGGAGTTCAATTTCATCCAGAAAAATCTGGTGATGCAGGAGCTCAATTGTTAAAAAATT
TTTTAGAGATATAAAAATCC

Downstream 100 bases:

>100_bases
TTGCATGTTTAGATGTTAACAATGGAGTTGTAGTAAAAGGCATTCAATTTAAAAACCATGAAATTGTAGGTGATATTATA
TCTTTGTCTAAACGTTATAC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIIPAFDLINGEIVRLYKGNFFNRTKYDINLYKRLKDYEIKGVKKIHLVDLDGAKNIKDRQIKVFRDIISYTNIPIQIGG
GIRNEEDINMFLNLGVKKVVIGSSAIKNKTKVKKWLKIYGPNVIILALDIIIKNNNKEIAIHGWQETTNITLEEIIEYFS
PIGLKHVLCTDISKDGTLSGPNNILYKDIVKKFKHISFQASGGVSRLKDLLSLKKTGVKSVIIGRSLLENKFTIEEALKC
WQSE

Sequences:

>Translated_244_residues
MIIPAFDLINGEIVRLYKGNFFNRTKYDINLYKRLKDYEIKGVKKIHLVDLDGAKNIKDRQIKVFRDIISYTNIPIQIGG
GIRNEEDINMFLNLGVKKVVIGSSAIKNKTKVKKWLKIYGPNVIILALDIIIKNNNKEIAIHGWQETTNITLEEIIEYFS
PIGLKHVLCTDISKDGTLSGPNNILYKDIVKKFKHISFQASGGVSRLKDLLSLKKTGVKSVIIGRSLLENKFTIEEALKC
WQSE
>Mature_244_residues
MIIPAFDLINGEIVRLYKGNFFNRTKYDINLYKRLKDYEIKGVKKIHLVDLDGAKNIKDRQIKVFRDIISYTNIPIQIGG
GIRNEEDINMFLNLGVKKVVIGSSAIKNKTKVKKWLKIYGPNVIILALDIIIKNNNKEIAIHGWQETTNITLEEIIEYFS
PIGLKHVLCTDISKDGTLSGPNNILYKDIVKKFKHISFQASGGVSRLKDLLSLKKTGVKSVIIGRSLLENKFTIEEALKC
WQSE

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=46.5020576131687, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BUCAP (Q9ZHE2)

Other databases:

- EMBL:   AF067228
- EMBL:   AE013218
- RefSeq:   NP_660456.1
- ProteinModelPortal:   Q9ZHE2
- EnsemblBacteria:   EBBUCT00000000094
- GeneID:   1005914
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg097
- GeneTree:   EBGT00050000007994
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   BAPH198804:BUSG097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27775; Mature: 27775

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: ACT_SITE 7-7 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPAFDLINGEIVRLYKGNFFNRTKYDINLYKRLKDYEIKGVKKIHLVDLDGAKNIKDR
CEECCEECCCCCEEEEEECCCCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
QIKVFRDIISYTNIPIQIGGGIRNEEDINMFLNLGVKKVVIGSSAIKNKTKVKKWLKIYG
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHC
PNVIILALDIIIKNNNKEIAIHGWQETTNITLEEIIEYFSPIGLKHVLCTDISKDGTLSG
CCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHEECCCCCCCCCC
PNNILYKDIVKKFKHISFQASGGVSRLKDLLSLKKTGVKSVIIGRSLLENKFTIEEALKC
CCCHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
WQSE
HCCC
>Mature Secondary Structure
MIIPAFDLINGEIVRLYKGNFFNRTKYDINLYKRLKDYEIKGVKKIHLVDLDGAKNIKDR
CEECCEECCCCCEEEEEECCCCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHH
QIKVFRDIISYTNIPIQIGGGIRNEEDINMFLNLGVKKVVIGSSAIKNKTKVKKWLKIYG
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHC
PNVIILALDIIIKNNNKEIAIHGWQETTNITLEEIIEYFSPIGLKHVLCTDISKDGTLSG
CCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHEECCCCCCCCCC
PNNILYKDIVKKFKHISFQASGGVSRLKDLLSLKKTGVKSVIIGRSLLENKFTIEEALKC
CCCHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
WQSE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9767718; 12089438