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The map label for this gene is hisH

Identifier: 21672388

GI number: 21672388

Start: 108958

End: 109551

Strand: Direct

Name: hisH

Synonym: BUsg096

Alternate gene names: 21672388

Gene position: 108958-109551 (Clockwise)

Preceding gene: 21672387

Following gene: 21672389

Centisome position: 16.99

GC content: 25.93

Gene sequence:

>594_bases
GTGGATATTGCAATATTAAATACTGGTTGTGCTAATTTAACTTCAATTCAAGTTGCAATTAAAAAATTAGGTTATGATTC
CATAGTAACTTCTGATCCTTCTATAGTATTAAAATCTAAAAAAATTTTTTTACCAGGTGTAGGAACAGCTTTAGCTGCTA
TGGAACAATTACATAAAAAAAATTTGATTAATACTTTAAAAAAATTGACGCAACCTATTCTAGGCATTTGTCTTGGAATG
CAAATTTTTTCTCAATTTAGCGAAGAATGTAAAGGAGTAAAAACTATTGGAATTTTTGATAATTGTTTTACGCATCTTTT
AAAAAGCAGTAATTTACCATTACCGCATATGGGTTGGAATCATATTGTTTTTAATAATTTGCATCCATTATTTAAAAACA
TTGATAAAAAAGAAAGATTTTATTTTGTTCATAGTTATATTATACCCCTGAATAAATATACTTTAGCAAAAACTAAATAT
GGTGTATCTTTTAGTTCTGTAATGCAAAAAAATAATTTTTTCGGAGTTCAATTTCATCCAGAAAAATCTGGTGATGCAGG
AGCTCAATTGTTAAAAAATTTTTTAGAGATATAA

Upstream 100 bases:

>100_bases
TCATTGTATTGAAAGTTTGTTTAAAGCATTTGGACGTGCACTCCGCAAGGCAGTAAAAATAGAAGGAAATACACTGCCAA
CATCTAAAGGGATTTTATAA

Downstream 100 bases:

>100_bases
AAATCCATGATTATTCCAGCATTTGATTTAATTAATGGCGAAATAGTTCGCTTGTATAAAGGAAATTTTTTTAATCGAAC
AAAATATGACATAAATTTAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MDIAILNTGCANLTSIQVAIKKLGYDSIVTSDPSIVLKSKKIFLPGVGTALAAMEQLHKKNLINTLKKLTQPILGICLGM
QIFSQFSEECKGVKTIGIFDNCFTHLLKSSNLPLPHMGWNHIVFNNLHPLFKNIDKKERFYFVHSYIIPLNKYTLAKTKY
GVSFSSVMQKNNFFGVQFHPEKSGDAGAQLLKNFLEI

Sequences:

>Translated_197_residues
MDIAILNTGCANLTSIQVAIKKLGYDSIVTSDPSIVLKSKKIFLPGVGTALAAMEQLHKKNLINTLKKLTQPILGICLGM
QIFSQFSEECKGVKTIGIFDNCFTHLLKSSNLPLPHMGWNHIVFNNLHPLFKNIDKKERFYFVHSYIIPLNKYTLAKTKY
GVSFSSVMQKNNFFGVQFHPEKSGDAGAQLLKNFLEI
>Mature_197_residues
MDIAILNTGCANLTSIQVAIKKLGYDSIVTSDPSIVLKSKKIFLPGVGTALAAMEQLHKKNLINTLKKLTQPILGICLGM
QIFSQFSEECKGVKTIGIFDNCFTHLLKSSNLPLPHMGWNHIVFNNLHPLFKNIDKKERFYFVHSYIIPLNKYTLAKTKY
GVSFSSVMQKNNFFGVQFHPEKSGDAGAQLLKNFLEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=51.7766497461929, Blast_Score=223, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=30.8056872037915, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BUCAP (Q9ZHE3)

Other databases:

- EMBL:   AF067228
- EMBL:   AE013218
- RefSeq:   NP_660455.1
- ProteinModelPortal:   Q9ZHE3
- SMR:   Q9ZHE3
- EnsemblBacteria:   EBBUCT00000000396
- GeneID:   1005913
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg096
- GeneTree:   EBGT00050000008173
- HOGENOM:   HBG292341
- OMA:   YAFERIC
- ProtClustDB:   PRK13170
- BioCyc:   BAPH198804:BUSG096-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22064; Mature: 22064

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 77-77 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAILNTGCANLTSIQVAIKKLGYDSIVTSDPSIVLKSKKIFLPGVGTALAAMEQLHKK
CCEEEEECCCCCHHHHHHHHHHCCCCHHCCCCCCEEEECCEEECCCCHHHHHHHHHHHHH
NLINTLKKLTQPILGICLGMQIFSQFSEECKGVKTIGIFDNCFTHLLKSSNLPLPHMGWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCC
HIVFNNLHPLFKNIDKKERFYFVHSYIIPLNKYTLAKTKYGVSFSSVMQKNNFFGVQFHP
CCHHCCCCHHHHCCCHHHHEEEEEHEEEECCCEEEHHHHCCCHHHHHHHCCCEEEEEECC
EKSGDAGAQLLKNFLEI
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDIAILNTGCANLTSIQVAIKKLGYDSIVTSDPSIVLKSKKIFLPGVGTALAAMEQLHKK
CCEEEEECCCCCHHHHHHHHHHCCCCHHCCCCCCEEEECCEEECCCCHHHHHHHHHHHHH
NLINTLKKLTQPILGICLGMQIFSQFSEECKGVKTIGIFDNCFTHLLKSSNLPLPHMGWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCC
HIVFNNLHPLFKNIDKKERFYFVHSYIIPLNKYTLAKTKYGVSFSSVMQKNNFFGVQFHP
CCHHCCCCHHHHCCCHHHHEEEEEHEEEECCCEEEHHHHCCCHHHHHHHCCCEEEEEECC
EKSGDAGAQLLKNFLEI
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9767718; 12089438