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The map label for this gene is pmbA

Identifier: 21672374

GI number: 21672374

Start: 91921

End: 93264

Strand: Direct

Name: pmbA

Synonym: BUsg082

Alternate gene names: 21672374

Gene position: 91921-93264 (Clockwise)

Preceding gene: 21672372

Following gene: 21672376

Centisome position: 14.33

GC content: 25.82

Gene sequence:

>1344_bases
ATGCAATTATTAAATCAAATAGAAAAAGAAGAAAATTTACTGATAGAAAAAGTAAAAAACACTCTTTTTCTAGCTCAAGA
AAAAGTTAATTGTTCTATCGAGGTTTGTGTTAAAAAAACAATAGGTATAAGTGTCAATATAAGAAATAGTATTGTTGAAA
ATGTAGAATTTAATAATGATAGTATACTTTTTGTTACAGTTTATAATAAGTTTTCTAAAGGTGTTTCATCCTCTAAAGAT
TTTAGCACCAACGGTATAAAAAAAATGTTAGAAATTGCAATTGACATTTCTAAACATACTTCTTCCGATTTTTTTTCAGG
ATTACCAGATGCAAAATTTTTATGCTTTAAAGCTCAAGATCTTGATTTATTTTATCCTTCTGAATTTAATATTAAAAATG
CAATTAATTTTGCTTCTGAATCAGAACGAGAAGCTTTTAAGTTTGACAAAAGAATTATAAATAGTGAAGGTAGTTTTTTT
AGTAATCATATTACGATAAAAGTTTTCGCTAATAGTTTAGGTATGTTAGAACAATATAAGTCTACTCGTTACTCTAACTA
TAACTGTATGATTGCGAAAGATAATAGTACTATGCAAAGAGACTTTTCTTATTCTACTTCAAGAAAATTAGATGATTTAG
AAAAACCAGATGTATTAGGTCAAAAAACTGCTACAAATGCTATATCTCGATTAGGTTCTAAAAAAATAAATACTATAAAA
TGTCCAGTAATTTTTTCAAAAGAAACATCTTCTATTTTTTTTTCTCATCTTATTCCAGCGATTAGTGGTGATAATATTTA
TCGAAAATCGACTTTTTTACTTTATGATTTAAAAAAAAGAATTTTCCCTGAATGGCTAAATATTGAAGAAAACCCACATA
TTAAAAAAGGATTAGGAAGTAAACCTTTTGATAGTGAAGGTGTATCAACAACTATTAGAAGTATTATTAAAAATGGAATT
TTGGAAACTTGGTTATTAAATACCTATAATGCTCGTAAATTAGGTTTAACAACTACAGGTCATTCTGGAGGTATTTCAAA
TTGGTTAGTTTCAAATAAAAATATATCTTTCAAAGAATTGTTAAAGATGATGAAAACTGGTTTACTAATCACTGAATTAA
TGGGACAAGGAGTTGATATTGTTAGTGGTAATTATTCACGTGGAGCAGTAGGATTTTGGATTGAAAACGGAAAAATTAAA
TATCCAGTAAATGAAATTACTATATCTGGTAATTTAAAAAAAATGTGGAATAGTATTCTTAGTATTAGTAATGATGTTAA
TATGTATAGTAATATTCAATGTGGTTCAACATTACTATCTGAAATTCAGATTTCAGGAAATTAA

Upstream 100 bases:

>100_bases
TTAAAAAAAATAATTTGTTTTGGATAATTATTTTTTTTTTTATATAATTATCTTTATTAAAAATAGTTTTATTAGTTAAT
ATATATTTAAGGACCTATAA

Downstream 100 bases:

>100_bases
TTTTTTATTTTTAAATAAATTTTTTAGTAAAAAGGTAATAAATTTATTACCTTTTATATTTTATTTTTTAAGAAGTTGAC
CTATAAGCCGGGTTCTGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MQLLNQIEKEENLLIEKVKNTLFLAQEKVNCSIEVCVKKTIGISVNIRNSIVENVEFNNDSILFVTVYNKFSKGVSSSKD
FSTNGIKKMLEIAIDISKHTSSDFFSGLPDAKFLCFKAQDLDLFYPSEFNIKNAINFASESEREAFKFDKRIINSEGSFF
SNHITIKVFANSLGMLEQYKSTRYSNYNCMIAKDNSTMQRDFSYSTSRKLDDLEKPDVLGQKTATNAISRLGSKKINTIK
CPVIFSKETSSIFFSHLIPAISGDNIYRKSTFLLYDLKKRIFPEWLNIEENPHIKKGLGSKPFDSEGVSTTIRSIIKNGI
LETWLLNTYNARKLGLTTTGHSGGISNWLVSNKNISFKELLKMMKTGLLITELMGQGVDIVSGNYSRGAVGFWIENGKIK
YPVNEITISGNLKKMWNSILSISNDVNMYSNIQCGSTLLSEIQISGN

Sequences:

>Translated_447_residues
MQLLNQIEKEENLLIEKVKNTLFLAQEKVNCSIEVCVKKTIGISVNIRNSIVENVEFNNDSILFVTVYNKFSKGVSSSKD
FSTNGIKKMLEIAIDISKHTSSDFFSGLPDAKFLCFKAQDLDLFYPSEFNIKNAINFASESEREAFKFDKRIINSEGSFF
SNHITIKVFANSLGMLEQYKSTRYSNYNCMIAKDNSTMQRDFSYSTSRKLDDLEKPDVLGQKTATNAISRLGSKKINTIK
CPVIFSKETSSIFFSHLIPAISGDNIYRKSTFLLYDLKKRIFPEWLNIEENPHIKKGLGSKPFDSEGVSTTIRSIIKNGI
LETWLLNTYNARKLGLTTTGHSGGISNWLVSNKNISFKELLKMMKTGLLITELMGQGVDIVSGNYSRGAVGFWIENGKIK
YPVNEITISGNLKKMWNSILSISNDVNMYSNIQCGSTLLSEIQISGN
>Mature_447_residues
MQLLNQIEKEENLLIEKVKNTLFLAQEKVNCSIEVCVKKTIGISVNIRNSIVENVEFNNDSILFVTVYNKFSKGVSSSKD
FSTNGIKKMLEIAIDISKHTSSDFFSGLPDAKFLCFKAQDLDLFYPSEFNIKNAINFASESEREAFKFDKRIINSEGSFF
SNHITIKVFANSLGMLEQYKSTRYSNYNCMIAKDNSTMQRDFSYSTSRKLDDLEKPDVLGQKTATNAISRLGSKKINTIK
CPVIFSKETSSIFFSHLIPAISGDNIYRKSTFLLYDLKKRIFPEWLNIEENPHIKKGLGSKPFDSEGVSTTIRSIIKNGI
LETWLLNTYNARKLGLTTTGHSGGISNWLVSNKNISFKELLKMMKTGLLITELMGQGVDIVSGNYSRGAVGFWIENGKIK
YPVNEITISGNLKKMWNSILSISNDVNMYSNIQCGSTLLSEIQISGN

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=51.006711409396, Blast_Score=503, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PMBA_BUCAP (Q8KA30)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660441.1
- ProteinModelPortal:   Q8KA30
- SMR:   Q8KA30
- MEROPS:   U62.001
- EnsemblBacteria:   EBBUCT00000000359
- GeneID:   1005899
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg082
- GeneTree:   EBGT00050000007696
- HOGENOM:   HBG726714
- OMA:   SRYSLSC
- ProtClustDB:   CLSK315232
- BioCyc:   BAPH198804:BUSG082-MONOMER
- GO:   GO:0005737
- InterPro:   IPR002510

Pfam domain/function: PF01523 PmbA_TldD

EC number: NA

Molecular weight: Translated: 50319; Mature: 50319

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLNQIEKEENLLIEKVKNTLFLAQEKVNCSIEVCVKKTIGISVNIRNSIVENVEFNND
CCHHHHHHHHHHHHHHHHHHHHEEEHHCCCCEEEEEEEECCCEEEEEHHHHHHCCEECCC
SILFVTVYNKFSKGVSSSKDFSTNGIKKMLEIAIDISKHTSSDFFSGLPDAKFLCFKAQD
CEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
LDLFYPSEFNIKNAINFASESEREAFKFDKRIINSEGSFFSNHITIKVFANSLGMLEQYK
CEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECEEEEEEEECHHHHHHHHH
STRYSNYNCMIAKDNSTMQRDFSYSTSRKLDDLEKPDVLGQKTATNAISRLGSKKINTIK
HCCCCCEEEEEECCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
CPVIFSKETSSIFFSHLIPAISGDNIYRKSTFLLYDLKKRIFPEWLNIEENPHIKKGLGS
CCEEECCCHHHHHHHHHHHHCCCCCEEECCEEHEEHHHHHHCHHHCCCCCCCCHHHCCCC
KPFDSEGVSTTIRSIIKNGILETWLLNTYNARKLGLTTTGHSGGISNWLVSNKNISFKEL
CCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCCCCHHHH
LKMMKTGLLITELMGQGVDIVSGNYSRGAVGFWIENGKIKYPVNEITISGNLKKMWNSIL
HHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCEEEECCEEEEECCCHHHHHHHHH
SISNDVNMYSNIQCGSTLLSEIQISGN
HCCCCCCHHCCCCHHHHHHHHEEECCC
>Mature Secondary Structure
MQLLNQIEKEENLLIEKVKNTLFLAQEKVNCSIEVCVKKTIGISVNIRNSIVENVEFNND
CCHHHHHHHHHHHHHHHHHHHHEEEHHCCCCEEEEEEEECCCEEEEEHHHHHHCCEECCC
SILFVTVYNKFSKGVSSSKDFSTNGIKKMLEIAIDISKHTSSDFFSGLPDAKFLCFKAQD
CEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
LDLFYPSEFNIKNAINFASESEREAFKFDKRIINSEGSFFSNHITIKVFANSLGMLEQYK
CEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECEEEEEEEECHHHHHHHHH
STRYSNYNCMIAKDNSTMQRDFSYSTSRKLDDLEKPDVLGQKTATNAISRLGSKKINTIK
HCCCCCEEEEEECCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
CPVIFSKETSSIFFSHLIPAISGDNIYRKSTFLLYDLKKRIFPEWLNIEENPHIKKGLGS
CCEEECCCHHHHHHHHHHHHCCCCCEEECCEEHEEHHHHHHCHHHCCCCCCCCHHHCCCC
KPFDSEGVSTTIRSIIKNGILETWLLNTYNARKLGLTTTGHSGGISNWLVSNKNISFKEL
CCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCCCCHHHH
LKMMKTGLLITELMGQGVDIVSGNYSRGAVGFWIENGKIKYPVNEITISGNLKKMWNSIL
HHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCEEEECCEEEEECCCHHHHHHHHH
SISNDVNMYSNIQCGSTLLSEIQISGN
HCCCCCCHHCCCCHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438