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The map label for this gene is argH

Identifier: 21672341

GI number: 21672341

Start: 55540

End: 56919

Strand: Direct

Name: argH

Synonym: BUsg048

Alternate gene names: 21672341

Gene position: 55540-56919 (Clockwise)

Preceding gene: 21672340

Following gene: 21672342

Centisome position: 8.66

GC content: 29.13

Gene sequence:

>1380_bases
ATGTCGCTTTGGGGTGGAAGATTCCTTGATGAATCTAATGAAACATTTAAAAAATTTAATGCCTCTTTACCTTTTGATTA
TATTTTAGTTAAAGAGGATATATTTGCATCTATTGCTTGGTCTAAATCTCTTGTTGAAGTTGGAGTTCTCACTCAGGAAG
AGCAAAAAAAAATAGAGTCTGCACTAATTTCTTTAAAAGAAGAAATTTATAATAATTCAAAAAAAATTCTTGAAAGTGAC
TACGAAGATATTCATAGTTGGATAGAAGCTAATCTTATTAAAAAAATAGGTGAATTAGGTAAAAAATTGCATACTGGTCG
TAGTAGAAATGATCAAATTACTACTGATTTAAAATTGTGGTGTAAAAGAAAAATTTTTGTTTTATTAGACAGTATTATTA
ATTTACAAAAAAATTTTATTTTTGTTGCTGAATTAAATGATGACGTCATAATGCCAGGATATACTCATTTGCAGCGTGCT
CAACCGATCACTTTTTCTTATTGGTGTTTAGCTTACGTACAAATGTTTAAACGTGATGTTAGTCGTTTAAAAGATATTTT
AAAAAGATTAGATATCAGTCCATTAGGTTCAGGTGCTCTATCTGGAACTGCATGGAATATTAATAGGAAAAAACTTGCTA
TATCAATGGGTTTTAGTTCGGAAACTAATAATGCACTTGATAGTGTTTCTGATAGAGATTATCTTATAGAATTGCTATCA
GCTTCTTCAATTAGTATGACTCATTTATCTCGTTTCTCTGAAGATTTAATTTTTTTTAACTCTAGTGAAGCTAATTTTAT
TGAATTATCTGATTCTATTACATCTGGATCGTCTTTAATGCCGCAAAAAAAAAATCCAGATGCATTAGAGCTTATACGTG
CTAAATGTGGTCGTGTTCATGGTGCTTTAGTCTCTATTTTAGTGGTTTTAAAATCTCTTCCCTTATCTTATAATAAAGAT
CTTCAAGAAGATAAAGAAGGTCTATTTGATTCTCTTAAAACTTGGAATGATTCTTTGTTGATAGCTAGTTTAGTTTTAAA
AAACATAAAATTAAATCGTTTATCGTGTCGTAAAGCTGCAGAAAAAGGTTATTCTAATGCCACGGAAATTGCAGATTATT
TAGTAAAAAAAGGTATGACTTTTCGTGAAGCACATCATGTATCAGGTAAATTAGTGCTTCGAGCTATTAAGGAAAACAAA
TCTTTGAATGATTTAGATCTCTCGATATTTCAAAGTTATAGTGCCCTTATTACTGATGATATATATAAAAATATTACGCT
AGAAGCATGTCTTGAAAAACGTTCTTCTAAAGGTGGAGTAGCACCAAATGAGATACGTAAAGAGATTTTTAAAGAAAAAG
AAAGATTAAATATTTTATAA

Upstream 100 bases:

>100_bases
GGTTTTATTCGTCTTTTTTCTCTTTCTTCTAGAATACGAGCTCAAAACAAGTTAAAATAAAAACATAAATATTTTTAAAT
CATTCTTATAGAGAAAATAT

Downstream 100 bases:

>100_bases
AAGATTAATTTTTAATTGAAGTTTTTTATTTTTAAATTTTATAAGGCATTTAAATTTTTTTTAAATGCCGTAATTAAAAT
TTATTTTATATAAAATTTTT

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSLWGGRFLDESNETFKKFNASLPFDYILVKEDIFASIAWSKSLVEVGVLTQEEQKKIESALISLKEEIYNNSKKILESD
YEDIHSWIEANLIKKIGELGKKLHTGRSRNDQITTDLKLWCKRKIFVLLDSIINLQKNFIFVAELNDDVIMPGYTHLQRA
QPITFSYWCLAYVQMFKRDVSRLKDILKRLDISPLGSGALSGTAWNINRKKLAISMGFSSETNNALDSVSDRDYLIELLS
ASSISMTHLSRFSEDLIFFNSSEANFIELSDSITSGSSLMPQKKNPDALELIRAKCGRVHGALVSILVVLKSLPLSYNKD
LQEDKEGLFDSLKTWNDSLLIASLVLKNIKLNRLSCRKAAEKGYSNATEIADYLVKKGMTFREAHHVSGKLVLRAIKENK
SLNDLDLSIFQSYSALITDDIYKNITLEACLEKRSSKGGVAPNEIRKEIFKEKERLNIL

Sequences:

>Translated_459_residues
MSLWGGRFLDESNETFKKFNASLPFDYILVKEDIFASIAWSKSLVEVGVLTQEEQKKIESALISLKEEIYNNSKKILESD
YEDIHSWIEANLIKKIGELGKKLHTGRSRNDQITTDLKLWCKRKIFVLLDSIINLQKNFIFVAELNDDVIMPGYTHLQRA
QPITFSYWCLAYVQMFKRDVSRLKDILKRLDISPLGSGALSGTAWNINRKKLAISMGFSSETNNALDSVSDRDYLIELLS
ASSISMTHLSRFSEDLIFFNSSEANFIELSDSITSGSSLMPQKKNPDALELIRAKCGRVHGALVSILVVLKSLPLSYNKD
LQEDKEGLFDSLKTWNDSLLIASLVLKNIKLNRLSCRKAAEKGYSNATEIADYLVKKGMTFREAHHVSGKLVLRAIKENK
SLNDLDLSIFQSYSALITDDIYKNITLEACLEKRSSKGGVAPNEIRKEIFKEKERLNIL
>Mature_458_residues
SLWGGRFLDESNETFKKFNASLPFDYILVKEDIFASIAWSKSLVEVGVLTQEEQKKIESALISLKEEIYNNSKKILESDY
EDIHSWIEANLIKKIGELGKKLHTGRSRNDQITTDLKLWCKRKIFVLLDSIINLQKNFIFVAELNDDVIMPGYTHLQRAQ
PITFSYWCLAYVQMFKRDVSRLKDILKRLDISPLGSGALSGTAWNINRKKLAISMGFSSETNNALDSVSDRDYLIELLSA
SSISMTHLSRFSEDLIFFNSSEANFIELSDSITSGSSLMPQKKNPDALELIRAKCGRVHGALVSILVVLKSLPLSYNKDL
QEDKEGLFDSLKTWNDSLLIASLVLKNIKLNRLSCRKAAEKGYSNATEIADYLVKKGMTFREAHHVSGKLVLRAIKENKS
LNDLDLSIFQSYSALITDDIYKNITLEACLEKRSSKGGVAPNEIRKEIFKEKERLNIL

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=447, Percent_Identity=42.0581655480984, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI68303542, Length=447, Percent_Identity=42.0581655480984, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI68303549, Length=447, Percent_Identity=40.0447427293065, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI68303547, Length=447, Percent_Identity=39.5973154362416, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1790398, Length=457, Percent_Identity=60.3938730853392, Blast_Score=610, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6321806, Length=458, Percent_Identity=43.2314410480349, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221473854, Length=447, Percent_Identity=40.0447427293065, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI78706858, Length=447, Percent_Identity=40.0447427293065, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_BUCAP (Q8KA59)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660408.1
- ProteinModelPortal:   Q8KA59
- SMR:   Q8KA59
- EnsemblBacteria:   EBBUCT00000000471
- GeneID:   1005864
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg048
- GeneTree:   EBGT00050000007810
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- ProtClustDB:   PRK04833
- BioCyc:   BAPH198804:BUSG048-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 52045; Mature: 51913

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLWGGRFLDESNETFKKFNASLPFDYILVKEDIFASIAWSKSLVEVGVLTQEEQKKIES
CCCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALISLKEEIYNNSKKILESDYEDIHSWIEANLIKKIGELGKKLHTGRSRNDQITTDLKLW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
CKRKIFVLLDSIINLQKNFIFVAELNDDVIMPGYTHLQRAQPITFSYWCLAYVQMFKRDV
HHHHHHHHHHHHHHHHHCEEEEEECCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
SRLKDILKRLDISPLGSGALSGTAWNINRKKLAISMGFSSETNNALDSVSDRDYLIELLS
HHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHH
ASSISMTHLSRFSEDLIFFNSSEANFIELSDSITSGSSLMPQKKNPDALELIRAKCGRVH
CCCHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GALVSILVVLKSLPLSYNKDLQEDKEGLFDSLKTWNDSLLIASLVLKNIKLNRLSCRKAA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
EKGYSNATEIADYLVKKGMTFREAHHVSGKLVLRAIKENKSLNDLDLSIFQSYSALITDD
HCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IYKNITLEACLEKRSSKGGVAPNEIRKEIFKEKERLNIL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLWGGRFLDESNETFKKFNASLPFDYILVKEDIFASIAWSKSLVEVGVLTQEEQKKIES
CCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALISLKEEIYNNSKKILESDYEDIHSWIEANLIKKIGELGKKLHTGRSRNDQITTDLKLW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
CKRKIFVLLDSIINLQKNFIFVAELNDDVIMPGYTHLQRAQPITFSYWCLAYVQMFKRDV
HHHHHHHHHHHHHHHHHCEEEEEECCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
SRLKDILKRLDISPLGSGALSGTAWNINRKKLAISMGFSSETNNALDSVSDRDYLIELLS
HHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHH
ASSISMTHLSRFSEDLIFFNSSEANFIELSDSITSGSSLMPQKKNPDALELIRAKCGRVH
CCCHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GALVSILVVLKSLPLSYNKDLQEDKEGLFDSLKTWNDSLLIASLVLKNIKLNRLSCRKAA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
EKGYSNATEIADYLVKKGMTFREAHHVSGKLVLRAIKENKSLNDLDLSIFQSYSALITDD
HCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IYKNITLEACLEKRSSKGGVAPNEIRKEIFKEKERLNIL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438