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The map label for this gene is argG

Identifier: 21672340

GI number: 21672340

Start: 54294

End: 55499

Strand: Direct

Name: argG

Synonym: BUsg047

Alternate gene names: 21672340

Gene position: 54294-55499 (Clockwise)

Preceding gene: 21672339

Following gene: 21672341

Centisome position: 8.46

GC content: 32.17

Gene sequence:

>1206_bases
ATGAATAATTTTAAAAAAGTGGTGTTAGCATATTCTGGTGGTTTAGATACTTCAGCCATCATCCCTTGGTTGAAAGAAAA
TTATCATGTTGATGTTGTGGCTTTTGTAGCTGATATAGGTCAATCAAAAAAAGATTTAAATGGTATTGAAAAAAAAGCAC
TGCAATCTGGTGCAATTGATTGCCGTGTTTTTGATTTAAAAGAGGAATTTATAAAAGATTACGTATATCCAGTTTTAAAA
ACAGGTTCTTTATATGAAGGAAATTATTTATTAGGTACAGCAATAGCTAGACCTATTATTGCTAAAAAACAAGTAGAATT
CGCTTCGAGTATTGGAGCAAAATTTTTATGTCATGGCGCTACTGGGAAGGGTAATGATCAAGTTCGTTTTGAGATAGCTT
ATGCAGCATTAGCTCCTTATATGAAAGTGATTGCTCCATGGCGCGAATGGAATCTTAATTCAAGAGAATCACTGTTAGAA
TATTTGCGTGAAAAAAATATTCCAACATCAGCGACTTTAGAGAAAATTTACAGCAGAGATGAGAATTCTTTGCATATTTC
TACAGAAGGAGGTTTACTTGAAGATCTTTGGAATAAACCGAATGCAGATTGTTGGAATTGGACAGTAGAACTAGAAGATG
CTCCAGAACAACCAGAATATATTTCTTTAATTGTAAAAAATGGATCTGTTGTATCTGTTAATAATGAGTTTTTAACTCCT
TTAAAATGTATAGAAAAATTAAACAAAATTGGTTCTAAACACGCTATAGGAAGAATTGACATTGTTGAAAATAGACTCGT
TGGAATTAAATCTAGAGGTTGCTATGAAACTCCGGGTGGTACTATTATGCACATAGCACTCAGAGCAATTGAGCAATTAG
TATTTGATCGAGAAAGTTTTAAATGGCGAGAAAAAATTGGTTTAGAAATGTCGTCTGTAGTTTACGATGGACGTTGGTTT
ACTCCTATTCGTAAATCTTTACAAGCTTCTGCAGATTCTTTATCTTCTGAAGTTAATGGCGAAGTTGTATTAAAATTGTA
TAAAGGCAGTGTAATTCCTGTACAAAAAAAATCTTCGAATTCTTTATATTCTAAAGAATATGCAACTTTTGGTGAAGATG
AAGTTTATAAGCATTCTGATGCTGAAGGTTTTATTCGTCTTTTTTCTCTTTCTTCTAGAATACGAGCTCAAAACAAGTTA
AAATAA

Upstream 100 bases:

>100_bases
AGTTGGCAAAATACAGATCAACTTAAATTATTATTTAATGGTATCAATATTGGTACTCGAGTTTTAGTATAGTCTGATTA
TAATAGGTTGTAAAAATATT

Downstream 100 bases:

>100_bases
AAACATAAATATTTTTAAATCATTCTTATAGAGAAAATATATGTCGCTTTGGGGTGGAAGATTCCTTGATGAATCTAATG
AAACATTTAAAAAATTTAAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNNFKKVVLAYSGGLDTSAIIPWLKENYHVDVVAFVADIGQSKKDLNGIEKKALQSGAIDCRVFDLKEEFIKDYVYPVLK
TGSLYEGNYLLGTAIARPIIAKKQVEFASSIGAKFLCHGATGKGNDQVRFEIAYAALAPYMKVIAPWREWNLNSRESLLE
YLREKNIPTSATLEKIYSRDENSLHISTEGGLLEDLWNKPNADCWNWTVELEDAPEQPEYISLIVKNGSVVSVNNEFLTP
LKCIEKLNKIGSKHAIGRIDIVENRLVGIKSRGCYETPGGTIMHIALRAIEQLVFDRESFKWREKIGLEMSSVVYDGRWF
TPIRKSLQASADSLSSEVNGEVVLKLYKGSVIPVQKKSSNSLYSKEYATFGEDEVYKHSDAEGFIRLFSLSSRIRAQNKL
K

Sequences:

>Translated_401_residues
MNNFKKVVLAYSGGLDTSAIIPWLKENYHVDVVAFVADIGQSKKDLNGIEKKALQSGAIDCRVFDLKEEFIKDYVYPVLK
TGSLYEGNYLLGTAIARPIIAKKQVEFASSIGAKFLCHGATGKGNDQVRFEIAYAALAPYMKVIAPWREWNLNSRESLLE
YLREKNIPTSATLEKIYSRDENSLHISTEGGLLEDLWNKPNADCWNWTVELEDAPEQPEYISLIVKNGSVVSVNNEFLTP
LKCIEKLNKIGSKHAIGRIDIVENRLVGIKSRGCYETPGGTIMHIALRAIEQLVFDRESFKWREKIGLEMSSVVYDGRWF
TPIRKSLQASADSLSSEVNGEVVLKLYKGSVIPVQKKSSNSLYSKEYATFGEDEVYKHSDAEGFIRLFSLSSRIRAQNKL
K
>Mature_401_residues
MNNFKKVVLAYSGGLDTSAIIPWLKENYHVDVVAFVADIGQSKKDLNGIEKKALQSGAIDCRVFDLKEEFIKDYVYPVLK
TGSLYEGNYLLGTAIARPIIAKKQVEFASSIGAKFLCHGATGKGNDQVRFEIAYAALAPYMKVIAPWREWNLNSRESLLE
YLREKNIPTSATLEKIYSRDENSLHISTEGGLLEDLWNKPNADCWNWTVELEDAPEQPEYISLIVKNGSVVSVNNEFLTP
LKCIEKLNKIGSKHAIGRIDIVENRLVGIKSRGCYETPGGTIMHIALRAIEQLVFDRESFKWREKIGLEMSSVVYDGRWF
TPIRKSLQASADSLSSEVNGEVVLKLYKGSVIPVQKKSSNSLYSKEYATFGEDEVYKHSDAEGFIRLFSLSSRIRAQNKL
K

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=42.1951219512195, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=42.1951219512195, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1789563, Length=409, Percent_Identity=25.9168704156479, Blast_Score=139, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=37.2881355932203, Blast_Score=264, Evalue=1e-71,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=40.0497512437811, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_BUCAP (Q8KA60)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660407.1
- ProteinModelPortal:   Q8KA60
- SMR:   Q8KA60
- EnsemblBacteria:   EBBUCT00000000444
- GeneID:   1005863
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg047
- GeneTree:   EBGT00050000008086
- HOGENOM:   HBG335267
- OMA:   ADLVTFE
- ProtClustDB:   PRK00509
- BioCyc:   BAPH198804:BUSG047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45124; Mature: 45124

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 89-89 BINDING 119-119 BINDING 121-121 BINDING 125-125 BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 178-178 BINDING 187-187 BINDING 263-263 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFKKVVLAYSGGLDTSAIIPWLKENYHVDVVAFVADIGQSKKDLNGIEKKALQSGAID
CCCCEEEEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCE
CRVFDLKEEFIKDYVYPVLKTGSLYEGNYLLGTAIARPIIAKKQVEFASSIGAKFLCHGA
EEEEHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECC
TGKGNDQVRFEIAYAALAPYMKVIAPWREWNLNSRESLLEYLREKNIPTSATLEKIYSRD
CCCCCCEEEEEEHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
ENSLHISTEGGLLEDLWNKPNADCWNWTVELEDAPEQPEYISLIVKNGSVVSVNNEFLTP
CCCEEEEECCCHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCEEEECCCCCCH
LKCIEKLNKIGSKHAIGRIDIVENRLVGIKSRGCYETPGGTIMHIALRAIEQLVFDRESF
HHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
KWREKIGLEMSSVVYDGRWFTPIRKSLQASADSLSSEVNGEVVLKLYKGSVIPVQKKSSN
HHHHHHCCCHHHEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCC
SLYSKEYATFGEDEVYKHSDAEGFIRLFSLSSRIRAQNKLK
CHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNFKKVVLAYSGGLDTSAIIPWLKENYHVDVVAFVADIGQSKKDLNGIEKKALQSGAID
CCCCEEEEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCE
CRVFDLKEEFIKDYVYPVLKTGSLYEGNYLLGTAIARPIIAKKQVEFASSIGAKFLCHGA
EEEEHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECC
TGKGNDQVRFEIAYAALAPYMKVIAPWREWNLNSRESLLEYLREKNIPTSATLEKIYSRD
CCCCCCEEEEEEHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
ENSLHISTEGGLLEDLWNKPNADCWNWTVELEDAPEQPEYISLIVKNGSVVSVNNEFLTP
CCCEEEEECCCHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCEEEECCCCCCH
LKCIEKLNKIGSKHAIGRIDIVENRLVGIKSRGCYETPGGTIMHIALRAIEQLVFDRESF
HHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
KWREKIGLEMSSVVYDGRWFTPIRKSLQASADSLSSEVNGEVVLKLYKGSVIPVQKKSSN
HHHHHHCCCHHHEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCC
SLYSKEYATFGEDEVYKHSDAEGFIRLFSLSSRIRAQNKLK
CHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438