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The map label for this gene is metF

Identifier: 21672336

GI number: 21672336

Start: 50249

End: 51127

Strand: Direct

Name: metF

Synonym: BUsg043

Alternate gene names: 21672336

Gene position: 50249-51127 (Clockwise)

Preceding gene: 21672324

Following gene: 21672338

Centisome position: 7.83

GC content: 24.69

Gene sequence:

>879_bases
ATGAACTTTTTACATCAGTATTATCAGGATATAATTAAGAAAAAATTAGAAAATTTAAATGATAAAATTCAATGTTCTTT
CGAATTTTTTCCTCCTAAAAATTTAGATACAGAAAAAAAATTATTCTCTTCAGTTGTAAAATTAAGTCTATTGAAACCGT
TTTTGTTTTCTGTCACTTATGGAGCAAATAGCGGAGAACGAAAAAAAACTTATAGTATTGTACAAAGAATACATAAAAAA
ACAGGTATTACAACAGCTCCTCATCTGACTTGTGTAGATGCTTCATTAAGTGAATTAAAAGAAATAGCAAAATTTTATTG
GGAAAATGGTATTAGAAGTATAGTTGCTTTAAGAGGAGACGTGCCAAACAAATCATATCAACATAAAATATATGCTTTAG
ATTTAGTTGTATTATTGAAGAAAATTGCTGATTTTGATATTTCTGTAGCTGCTTATCCAGAAATACATCCAGAATCAAAA
AATGCTCAATCTGATATTTTAAATTTAAAAAAGAAAGCTGATGCTGGTGCAAATAGAGCAATTACTCAGTTTTTTTTTAA
TGTTGAAAGTTATTTACGTTTTCGTGATAACTGTATAAAAAATAATATAAATATTGAAATTATACCTGGTATACTACCAA
TTTATAATTTTGAACAACTAAAAAAATTTTCTAGTATGACTAATGTGAGTATTCCTAAATGGATGTTTGAAATGTTTAAC
GGATTAGATGAAGATTTAGAAACTCAGAAAATTATAGGTTCAAACATAGTTATAAATATTGTAAAAACGTTATTATATGA
AGGAGTAAAAAATTTTCATTTTTATACTTTAAATAAATCAGATGTAATATATTCTATATGTCATATGTTTGGTTTATAA

Upstream 100 bases:

>100_bases
AATTTAATTCTTTAAACTTTAAAAAGTTAATATAAATTTAATGTATAATATATCAAAAATATTAAGTTTTAACTATCAAA
TTTTAATGGAATTTTTAATA

Downstream 100 bases:

>100_bases
TAATCAAAGAAATCATTTTTTTTAAATATTATTTAATAACAAAAGTTTTTTATTAATTTTCTTATTATTTTTTTAGTAGG
TTTAATAAAAGAACAATCTA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTYGANSGERKKTYSIVQRIHKK
TGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGDVPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESK
NAQSDILNLKKKADAGANRAITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFN
GLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL

Sequences:

>Translated_292_residues
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTYGANSGERKKTYSIVQRIHKK
TGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGDVPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESK
NAQSDILNLKKKADAGANRAITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFN
GLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL
>Mature_292_residues
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTYGANSGERKKTYSIVQRIHKK
TGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGDVPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESK
NAQSDILNLKKKADAGANRAITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFN
GLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family

Homologues:

Organism=Homo sapiens, GI87240000, Length=299, Percent_Identity=29.0969899665552, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1790377, Length=292, Percent_Identity=59.5890410958904, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25147441, Length=300, Percent_Identity=30, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147436, Length=300, Percent_Identity=30, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=283, Percent_Identity=30.7420494699647, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=283, Percent_Identity=28.9752650176678, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METF_BUCAP (Q8KA62)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660403.1
- ProteinModelPortal:   Q8KA62
- SMR:   Q8KA62
- EnsemblBacteria:   EBBUCT00000000433
- GeneID:   1005859
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg043
- GeneTree:   EBGT00050000007865
- HOGENOM:   HBG649727
- OMA:   ITQMFFD
- ProtClustDB:   PRK09432
- BioCyc:   BAPH198804:BUSG043-MONOMER
- InterPro:   IPR003171
- InterPro:   IPR004620
- TIGRFAMs:   TIGR00676

Pfam domain/function: PF02219 MTHFR

EC number: =1.5.1.20

Molecular weight: Translated: 33676; Mature: 33676

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTY
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
GANSGERKKTYSIVQRIHKKTGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESKNAQSDILNLKKKADAGANRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFN
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHC
GLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MNFLHQYYQDIIKKKLENLNDKIQCSFEFFPPKNLDTEKKLFSSVVKLSLLKPFLFSVTY
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
GANSGERKKTYSIVQRIHKKTGITTAPHLTCVDASLSELKEIAKFYWENGIRSIVALRGD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPNKSYQHKIYALDLVVLLKKIADFDISVAAYPEIHPESKNAQSDILNLKKKADAGANRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ITQFFFNVESYLRFRDNCIKNNINIEIIPGILPIYNFEQLKKFSSMTNVSIPKWMFEMFN
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHC
GLDEDLETQKIIGSNIVINIVKTLLYEGVKNFHFYTLNKSDVIYSICHMFGL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438