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The map label for this gene is metE

Identifier: 21672324

GI number: 21672324

Start: 31532

End: 33805

Strand: Direct

Name: metE

Synonym: BUsg031

Alternate gene names: 21672324

Gene position: 31532-33805 (Clockwise)

Preceding gene: 21672322

Following gene: 21672336

Centisome position: 4.92

GC content: 28.54

Gene sequence:

>2274_bases
ATGACTATTTTTAATCATACTCTTGGATTTCCAAGAATAGGTTTAAATCGTGAATTAAAAAAAGCTCAGGAAGATTACTG
GTCTGGAAATCTTCTATATGAAGATTTTTTGTGCGTGGGGTCTCAATTAAGAAGAGAGAATTGGAAAAAACAAAAAGACG
CCGGAATTGATTACATACCAGTTGGAGATTTTGCTTGGTATGATCATGTATTAAGTACCAGCATGATGTTAGGAAACATT
CCTGAAAGACATCTTAATGTGAATGATAATAAGATTGATTTAGATTGTTTATTTCGAATTGCACGCGGTTGTTCTCCAGA
TGTTGCTGCTTCAGAAATGACTAAATGGTTTAATACCAATTATCATTATATAGTACCAGAATTCAATAAAAATAGAATAT
TAAAATTTTCTTGGAAACAAATTTTAGAAGAAACTGATGAAGCATTATTATTAGGATATAAAGTCAAACCTATAATTTTA
GGTCCAATTACATATCTTTGGTTAGGAAAGATAAAAGGAGAATATTTTGATCGTTTAGATCTTCTTAAAAATATATTACC
AATATATAAACAGGTTTTAAGAGAATTGTCACAACGTAATATTGATTTTGTTCAAATTGATGAACCTGCTTTAGTTTTAG
ATCTTCCAGAAAAGTGGAAAAAAGCTTATTCTTACGCTTATGAATATTTAAATGGAACAACTAAAATATTATTAACAACA
TACTTTGACAGTATTGAACATAACATAGAATATATTCGAGATTTACCGATTTATGGTATTCACATTGATTTAGTTTTTGG
AAAATATAACTTATTTGATTTTAATTCTAAATTACCGAAAGAATGGATTTTATCTTTAGGAGTCATTAATGGTCGAAATG
TATGGCGTGCTGATCTTATAAAATTATTTAAAAGTATTTCTACTATTTTAAAATTTCGTAAAAAATTATTAATTGGTTCA
TCTTGTTCTTTATTGCATTCTCCTATCGATTTACAAAAAGAAAAAAATCTAGATAAAGAATCTAAAAAATGGTTTTCTTT
TGCAGTACAAAAGTGTACTGAATTATCTTTGTTATCAAAAGCGTTAAATAAAAATGATACAGATTCAATTCAAAAATGGT
GTTCATCTATTTATGAACGTAATTTTTCTAAAAGAGTGCAAAAAATTGAAGTTCAAAACCGTTTATCTGAAATTTTGAAT
GTTAATTTTAAACGTTCTGATTCGTATAATATTCGTTCTCGAGAGCAGAAAAAGAAATTTAATTTACCTATTTTGCCAAC
AACGACTATTGGATCATTCCCTCAGACACTACAAATAAGAAAATTACGACGTGATTATAAACAAGGATTAATCAATGATG
TTGATTATGAAATAGGTATTAAAAAACATATTAAAGAAGTAATAAAAGAGCAAGAAAAACTAGGTATAGATGTCTTAGTT
CATGGAGAACCTGAAAGAAATGACATGGTTGAATATTTTGGTGAAAATTTAGATGGATTTATATTTACAGATAACGGATG
GGTTCAAAGTTATGGATCACGTTGCGTGAAACCACCAATTATTATTGGTGATGTTAGTCGTTTAAAACCAATAACTGTGA
AATGGTCTAAATATGCTCAATCTTTAACAGATAAACCAGTAAAAGCGATGCTAACTGGACCAGTAACTATTTTATTTTGG
TCTTTTCCTAGAGAAGATATTTCATTAGAAAAAATTGCTAAACAAATCGGGTTGGCATTGCGAGATGAAGTGTTAGATTT
AGAAAAAGAAAAAATAGAAATCATTCAAATTGATGAACCTGCATTACGAGAAGGCTTGCCTTTGCGTAAAAGTTTATGGA
ATGAATATTTGTCTTGGGCAGTAGATGCTTTTCGCTTGAGTTCTTCAGGTGTAAAAAATACTACTCAAATTCACACACAT
ATGTGTTATTGTGAATTTCATGATATCATGCACGCTATATCTTTCTTAGACGCTGATGTTATTACAATTGAAACTGCTCG
TTCAGATATGGAATTACTGAAATCGTTTAAAACATTTAAATATCCAAATGAAATTGGTCCTGGAGTTTATGATATTCATT
CCTCAAACATACCTAGTATTACATCAATAAAATTTTTATTAAATAAAGCAATAGCTTATATTCCAGTAGAACGTATTTGG
GTAAATCCCGATTGTGGTTTAAAAACTCGTAATTGGAATGAAACAATATTAGCATTAAAAAATATGGTTTGTGCTGCAAA
AGAAATGAGAGATAAAATAAAAAAAATAACTTAA

Upstream 100 bases:

>100_bases
AATTTTACTGGATATGAGAATTATAAGATATCTGATTTAAATAAATCAATATATAAAATTTTATTAATTGATAATTTTAT
TATGAATGATAAGGAAAAAG

Downstream 100 bases:

>100_bases
ATAAATAAAATATTTTTTCTATAAAATATAAATTTTAATTAAATCATCTTTTAGATTATTTTATTAGAAATAAAAATCTA
AATGATGATTTAATAAATTT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MTIFNHTLGFPRIGLNRELKKAQEDYWSGNLLYEDFLCVGSQLRRENWKKQKDAGIDYIPVGDFAWYDHVLSTSMMLGNI
PERHLNVNDNKIDLDCLFRIARGCSPDVAASEMTKWFNTNYHYIVPEFNKNRILKFSWKQILEETDEALLLGYKVKPIIL
GPITYLWLGKIKGEYFDRLDLLKNILPIYKQVLRELSQRNIDFVQIDEPALVLDLPEKWKKAYSYAYEYLNGTTKILLTT
YFDSIEHNIEYIRDLPIYGIHIDLVFGKYNLFDFNSKLPKEWILSLGVINGRNVWRADLIKLFKSISTILKFRKKLLIGS
SCSLLHSPIDLQKEKNLDKESKKWFSFAVQKCTELSLLSKALNKNDTDSIQKWCSSIYERNFSKRVQKIEVQNRLSEILN
VNFKRSDSYNIRSREQKKKFNLPILPTTTIGSFPQTLQIRKLRRDYKQGLINDVDYEIGIKKHIKEVIKEQEKLGIDVLV
HGEPERNDMVEYFGENLDGFIFTDNGWVQSYGSRCVKPPIIIGDVSRLKPITVKWSKYAQSLTDKPVKAMLTGPVTILFW
SFPREDISLEKIAKQIGLALRDEVLDLEKEKIEIIQIDEPALREGLPLRKSLWNEYLSWAVDAFRLSSSGVKNTTQIHTH
MCYCEFHDIMHAISFLDADVITIETARSDMELLKSFKTFKYPNEIGPGVYDIHSSNIPSITSIKFLLNKAIAYIPVERIW
VNPDCGLKTRNWNETILALKNMVCAAKEMRDKIKKIT

Sequences:

>Translated_757_residues
MTIFNHTLGFPRIGLNRELKKAQEDYWSGNLLYEDFLCVGSQLRRENWKKQKDAGIDYIPVGDFAWYDHVLSTSMMLGNI
PERHLNVNDNKIDLDCLFRIARGCSPDVAASEMTKWFNTNYHYIVPEFNKNRILKFSWKQILEETDEALLLGYKVKPIIL
GPITYLWLGKIKGEYFDRLDLLKNILPIYKQVLRELSQRNIDFVQIDEPALVLDLPEKWKKAYSYAYEYLNGTTKILLTT
YFDSIEHNIEYIRDLPIYGIHIDLVFGKYNLFDFNSKLPKEWILSLGVINGRNVWRADLIKLFKSISTILKFRKKLLIGS
SCSLLHSPIDLQKEKNLDKESKKWFSFAVQKCTELSLLSKALNKNDTDSIQKWCSSIYERNFSKRVQKIEVQNRLSEILN
VNFKRSDSYNIRSREQKKKFNLPILPTTTIGSFPQTLQIRKLRRDYKQGLINDVDYEIGIKKHIKEVIKEQEKLGIDVLV
HGEPERNDMVEYFGENLDGFIFTDNGWVQSYGSRCVKPPIIIGDVSRLKPITVKWSKYAQSLTDKPVKAMLTGPVTILFW
SFPREDISLEKIAKQIGLALRDEVLDLEKEKIEIIQIDEPALREGLPLRKSLWNEYLSWAVDAFRLSSSGVKNTTQIHTH
MCYCEFHDIMHAISFLDADVITIETARSDMELLKSFKTFKYPNEIGPGVYDIHSSNIPSITSIKFLLNKAIAYIPVERIW
VNPDCGLKTRNWNETILALKNMVCAAKEMRDKIKKIT
>Mature_756_residues
TIFNHTLGFPRIGLNRELKKAQEDYWSGNLLYEDFLCVGSQLRRENWKKQKDAGIDYIPVGDFAWYDHVLSTSMMLGNIP
ERHLNVNDNKIDLDCLFRIARGCSPDVAASEMTKWFNTNYHYIVPEFNKNRILKFSWKQILEETDEALLLGYKVKPIILG
PITYLWLGKIKGEYFDRLDLLKNILPIYKQVLRELSQRNIDFVQIDEPALVLDLPEKWKKAYSYAYEYLNGTTKILLTTY
FDSIEHNIEYIRDLPIYGIHIDLVFGKYNLFDFNSKLPKEWILSLGVINGRNVWRADLIKLFKSISTILKFRKKLLIGSS
CSLLHSPIDLQKEKNLDKESKKWFSFAVQKCTELSLLSKALNKNDTDSIQKWCSSIYERNFSKRVQKIEVQNRLSEILNV
NFKRSDSYNIRSREQKKKFNLPILPTTTIGSFPQTLQIRKLRRDYKQGLINDVDYEIGIKKHIKEVIKEQEKLGIDVLVH
GEPERNDMVEYFGENLDGFIFTDNGWVQSYGSRCVKPPIIIGDVSRLKPITVKWSKYAQSLTDKPVKAMLTGPVTILFWS
FPREDISLEKIAKQIGLALRDEVLDLEKEKIEIIQIDEPALREGLPLRKSLWNEYLSWAVDAFRLSSSGVKNTTQIHTHM
CYCEFHDIMHAISFLDADVITIETARSDMELLKSFKTFKYPNEIGPGVYDIHSSNIPSITSIKFLLNKAIAYIPVERIWV
NPDCGLKTRNWNETILALKNMVCAAKEMRDKIKKIT

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=63.0810092961487, Blast_Score=1023, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=770, Percent_Identity=45.1948051948052, Blast_Score=659, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BUCAP (Q8KA71)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660391.1
- ProteinModelPortal:   Q8KA71
- SMR:   Q8KA71
- EnsemblBacteria:   EBBUCT00000000544
- GeneID:   1005847
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg031
- GeneTree:   EBGT00050000007816
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   BAPH198804:BUSG031-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 88099; Mature: 87968

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFNHTLGFPRIGLNRELKKAQEDYWSGNLLYEDFLCVGSQLRRENWKKQKDAGIDYIP
CCEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VGDFAWYDHVLSTSMMLGNIPERHLNVNDNKIDLDCLFRIARGCSPDVAASEMTKWFNTN
CCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCC
YHYIVPEFNKNRILKFSWKQILEETDEALLLGYKVKPIILGPITYLWLGKIKGEYFDRLD
CEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEEECEEEECCHHHHHHHHHCHHHHHHHH
LLKNILPIYKQVLRELSQRNIDFVQIDEPALVLDLPEKWKKAYSYAYEYLNGTTKILLTT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCEEEHHH
YFDSIEHNIEYIRDLPIYGIHIDLVFGKYNLFDFNSKLPKEWILSLGVINGRNVWRADLI
HHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHH
KLFKSISTILKFRKKLLIGSSCSLLHSPIDLQKEKNLDKESKKWFSFAVQKCTELSLLSK
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALNKNDTDSIQKWCSSIYERNFSKRVQKIEVQNRLSEILNVNFKRSDSYNIRSREQKKKF
HHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
NLPILPTTTIGSFPQTLQIRKLRRDYKQGLINDVDYEIGIKKHIKEVIKEQEKLGIDVLV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
HGEPERNDMVEYFGENLDGFIFTDNGWVQSYGSRCVKPPIIIGDVSRLKPITVKWSKYAQ
ECCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEECHHHCCCEEEEHHHHHH
SLTDKPVKAMLTGPVTILFWSFPREDISLEKIAKQIGLALRDEVLDLEKEKIEIIQIDEP
HHCCCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
ALREGLPLRKSLWNEYLSWAVDAFRLSSSGVKNTTQIHTHMCYCEFHDIMHAISFLDADV
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
ITIETARSDMELLKSFKTFKYPNEIGPGVYDIHSSNIPSITSIKFLLNKAIAYIPVERIW
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
VNPDCGLKTRNWNETILALKNMVCAAKEMRDKIKKIT
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIFNHTLGFPRIGLNRELKKAQEDYWSGNLLYEDFLCVGSQLRRENWKKQKDAGIDYIP
CEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VGDFAWYDHVLSTSMMLGNIPERHLNVNDNKIDLDCLFRIARGCSPDVAASEMTKWFNTN
CCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCC
YHYIVPEFNKNRILKFSWKQILEETDEALLLGYKVKPIILGPITYLWLGKIKGEYFDRLD
CEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEEECEEEECCHHHHHHHHHCHHHHHHHH
LLKNILPIYKQVLRELSQRNIDFVQIDEPALVLDLPEKWKKAYSYAYEYLNGTTKILLTT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCEEEHHH
YFDSIEHNIEYIRDLPIYGIHIDLVFGKYNLFDFNSKLPKEWILSLGVINGRNVWRADLI
HHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHH
KLFKSISTILKFRKKLLIGSSCSLLHSPIDLQKEKNLDKESKKWFSFAVQKCTELSLLSK
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALNKNDTDSIQKWCSSIYERNFSKRVQKIEVQNRLSEILNVNFKRSDSYNIRSREQKKKF
HHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
NLPILPTTTIGSFPQTLQIRKLRRDYKQGLINDVDYEIGIKKHIKEVIKEQEKLGIDVLV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
HGEPERNDMVEYFGENLDGFIFTDNGWVQSYGSRCVKPPIIIGDVSRLKPITVKWSKYAQ
ECCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEECHHHCCCEEEEHHHHHH
SLTDKPVKAMLTGPVTILFWSFPREDISLEKIAKQIGLALRDEVLDLEKEKIEIIQIDEP
HHCCCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
ALREGLPLRKSLWNEYLSWAVDAFRLSSSGVKNTTQIHTHMCYCEFHDIMHAISFLDADV
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
ITIETARSDMELLKSFKTFKYPNEIGPGVYDIHSSNIPSITSIKFLLNKAIAYIPVERIW
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
VNPDCGLKTRNWNETILALKNMVCAAKEMRDKIKKIT
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438