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The map label for this gene is trmE

Identifier: 21672311

GI number: 21672311

Start: 16573

End: 17943

Strand: Direct

Name: trmE

Synonym: BUsg017

Alternate gene names: 21672311

Gene position: 16573-17943 (Clockwise)

Preceding gene: 21672310

Following gene: 21672312

Centisome position: 2.58

GC content: 26.84

Gene sequence:

>1371_bases
ATGATTCGAAATGATACTATTATTGCTCAAGTTACCTGTCCTGGAAAAAGTGCTGTTGGAATATTAAGAGTTTCAGGTAT
TCATGCCAATCAAGTTGCTTTTGCAGTGTTAGGTAAAATCCCTAAACCAAGATTTGCTACTTATTCAAAGTTTTTTGATG
AAAGTAAAAAGGTATTAGATGAAGGTATATCTTTATGGTTTCCAGCACCTTTTTCTTTAACAGGAGAAGATGTATTAGAA
CTACAAGGTCATGGTAATCCATTTATTATGGACTTACTAATAAAAAGAATTTTATGTTTGAAAAATATTAAAATAAGAAT
TGCTCAACCAGGTGAATTTTGTCAACGCGCATTTTTAAATGGGAAAATAGATTTGATTCAAGCAGAAGCAATAGATGATT
TAATTAATTCTGAAACAGAGTCCGTGGTTCGAGCGTCCTTAAATTCTTTACATGGAAATTTTTCATTTTATATTCAAAAA
ATAATAAAAAAATTAATTGAATTTCGTACTAATATAGAAGCAAGTATAGATTTTTCAGAAGAAAATATTGATTTTGATTT
TAATATTTTTATTATGTCAAATTTTGAAAAATTGAATGATAAATTTTTAAAAATAAAAAATATAGTTTCAGAAGGTAGTT
TGATAAGAGAAGCAAAAAGAATAGTAATTGTCGGACCACCGAATGCTGGAAAATCTAGTTTGTTAAATGTTTTATCATGT
CGTGATAGAGCTATAGTAACTGATTTACCTGGTACAACACGCGACGTTCTTTATGAAAATATAAATATTCATGGTATTTC
ATGTGAAATAATTGATACTGCGGGTTTACGTGAAACAGAAGATAAAATTGAAAAAATTGGAATACAACGTTCTTGGGAGA
TGATCAAAAATTCTGATCATGTTCTATATGTGATGGATAAAACAATTAGTTTAGAAGATCAAAAAAAAACATCTATTCAG
TTTATGAAACAAATTTCTTCTTATAACATACAAGAAGTGACTTTTGTTTTGAATAAAAATGATTTAGTAGAAGATTTTTG
TGGAATTACAAAAATTGAAAATTTACTATTTATCAGTATTTCTGCTCTTACGGGTCAAGGAATTGATATTTTAAAAAAAC
ATCTTTCAAACAGACAAAAAGATAAAAGTCAAGAAGGTCTTTTTATTGCTAGAAGACGTCATATTCATCAAATTGATCTA
TCGTATTGTGAACTTTTAAAAGCTCAAAAAAATTGGTTAAAATATAAAAATATTGAATTATTAGCTGAATCACTCAATAT
AATTAATAAATTATTAGGAGAAATAACAGGTGAGTTTACTTCTAGTGATTTATTAAAACGTATTTTTTCTACCTTTTGCA
TAGGAAAATAA

Upstream 100 bases:

>100_bases
TACTATTATACAACAAAAATATATTTTATCTAATTTTAAAAAAAATCAAAAAAGATAAAAATTATTTATTCTTTTTATTT
AAAAATTAAGAAAATATTTT

Downstream 100 bases:

>100_bases
GAATATAATTTTTGCCCGGAGGCGGAATTGAACCACCGACACGGGGATTTTCAGTCCCCTGCTCTACCGACTGAGCTATC
CGGGCAATTTTTACATTAAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIRNDTIIAQVTCPGKSAVGILRVSGIHANQVAFAVLGKIPKPRFATYSKFFDESKKVLDEGISLWFPAPFSLTGEDVLE
LQGHGNPFIMDLLIKRILCLKNIKIRIAQPGEFCQRAFLNGKIDLIQAEAIDDLINSETESVVRASLNSLHGNFSFYIQK
IIKKLIEFRTNIEASIDFSEENIDFDFNIFIMSNFEKLNDKFLKIKNIVSEGSLIREAKRIVIVGPPNAGKSSLLNVLSC
RDRAIVTDLPGTTRDVLYENINIHGISCEIIDTAGLRETEDKIEKIGIQRSWEMIKNSDHVLYVMDKTISLEDQKKTSIQ
FMKQISSYNIQEVTFVLNKNDLVEDFCGITKIENLLFISISALTGQGIDILKKHLSNRQKDKSQEGLFIARRRHIHQIDL
SYCELLKAQKNWLKYKNIELLAESLNIINKLLGEITGEFTSSDLLKRIFSTFCIGK

Sequences:

>Translated_456_residues
MIRNDTIIAQVTCPGKSAVGILRVSGIHANQVAFAVLGKIPKPRFATYSKFFDESKKVLDEGISLWFPAPFSLTGEDVLE
LQGHGNPFIMDLLIKRILCLKNIKIRIAQPGEFCQRAFLNGKIDLIQAEAIDDLINSETESVVRASLNSLHGNFSFYIQK
IIKKLIEFRTNIEASIDFSEENIDFDFNIFIMSNFEKLNDKFLKIKNIVSEGSLIREAKRIVIVGPPNAGKSSLLNVLSC
RDRAIVTDLPGTTRDVLYENINIHGISCEIIDTAGLRETEDKIEKIGIQRSWEMIKNSDHVLYVMDKTISLEDQKKTSIQ
FMKQISSYNIQEVTFVLNKNDLVEDFCGITKIENLLFISISALTGQGIDILKKHLSNRQKDKSQEGLFIARRRHIHQIDL
SYCELLKAQKNWLKYKNIELLAESLNIINKLLGEITGEFTSSDLLKRIFSTFCIGK
>Mature_456_residues
MIRNDTIIAQVTCPGKSAVGILRVSGIHANQVAFAVLGKIPKPRFATYSKFFDESKKVLDEGISLWFPAPFSLTGEDVLE
LQGHGNPFIMDLLIKRILCLKNIKIRIAQPGEFCQRAFLNGKIDLIQAEAIDDLINSETESVVRASLNSLHGNFSFYIQK
IIKKLIEFRTNIEASIDFSEENIDFDFNIFIMSNFEKLNDKFLKIKNIVSEGSLIREAKRIVIVGPPNAGKSSLLNVLSC
RDRAIVTDLPGTTRDVLYENINIHGISCEIIDTAGLRETEDKIEKIGIQRSWEMIKNSDHVLYVMDKTISLEDQKKTSIQ
FMKQISSYNIQEVTFVLNKNDLVEDFCGITKIENLLFISISALTGQGIDILKKHLSNRQKDKSQEGLFIARRRHIHQIDL
SYCELLKAQKNWLKYKNIELLAESLNIINKLLGEITGEFTSSDLLKRIFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=479, Percent_Identity=31.9415448851775, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=30.2186878727634, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI193082993, Length=471, Percent_Identity=31.4225053078556, Blast_Score=216, Evalue=5e-56,
Organism=Escherichia coli, GI2367268, Length=458, Percent_Identity=48.471615720524, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI87082120, Length=106, Percent_Identity=34.9056603773585, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=457, Percent_Identity=29.9781181619256, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=484, Percent_Identity=34.297520661157, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=465, Percent_Identity=30.9677419354839, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BUCAP (Q44633)

Other databases:

- EMBL:   D85628
- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660378.1
- ProteinModelPortal:   Q44633
- SMR:   Q44633
- EnsemblBacteria:   EBBUCT00000000166
- GeneID:   1005833
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg017
- GeneTree:   EBGT00050000007774
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   BAPH198804:BUSG017-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51696; Mature: 51696

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 122-122 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRNDTIIAQVTCPGKSAVGILRVSGIHANQVAFAVLGKIPKPRFATYSKFFDESKKVLD
CCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EGISLWFPAPFSLTGEDVLELQGHGNPFIMDLLIKRILCLKNIKIRIAQPGEFCQRAFLN
CCCCEEECCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCC
GKIDLIQAEAIDDLINSETESVVRASLNSLHGNFSFYIQKIIKKLIEFRTNIEASIDFSE
CCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
ENIDFDFNIFIMSNFEKLNDKFLKIKNIVSEGSLIREAKRIVIVGPPNAGKSSLLNVLSC
CCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHC
RDRAIVTDLPGTTRDVLYENINIHGISCEIIDTAGLRETEDKIEKIGIQRSWEMIKNSDH
CCCEEEECCCCCHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHCCCCC
VLYVMDKTISLEDQKKTSIQFMKQISSYNIQEVTFVLNKNDLVEDFCGITKIENLLFISI
EEEEEECEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCEEEEEE
SALTGQGIDILKKHLSNRQKDKSQEGLFIARRRHIHQIDLSYCELLKAQKNWLKYKNIEL
ECCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAESLNIINKLLGEITGEFTSSDLLKRIFSTFCIGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRNDTIIAQVTCPGKSAVGILRVSGIHANQVAFAVLGKIPKPRFATYSKFFDESKKVLD
CCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EGISLWFPAPFSLTGEDVLELQGHGNPFIMDLLIKRILCLKNIKIRIAQPGEFCQRAFLN
CCCCEEECCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCC
GKIDLIQAEAIDDLINSETESVVRASLNSLHGNFSFYIQKIIKKLIEFRTNIEASIDFSE
CCEEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
ENIDFDFNIFIMSNFEKLNDKFLKIKNIVSEGSLIREAKRIVIVGPPNAGKSSLLNVLSC
CCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHC
RDRAIVTDLPGTTRDVLYENINIHGISCEIIDTAGLRETEDKIEKIGIQRSWEMIKNSDH
CCCEEEECCCCCHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHCCCCC
VLYVMDKTISLEDQKKTSIQFMKQISSYNIQEVTFVLNKNDLVEDFCGITKIENLLFISI
EEEEEECEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCEEEEEE
SALTGQGIDILKKHLSNRQKDKSQEGLFIARRRHIHQIDLSYCELLKAQKNWLKYKNIEL
ECCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAESLNIINKLLGEITGEFTSSDLLKRIFSTFCIGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438