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The map label for this gene is yhfT [H]

Identifier: 30263574

GI number: 30263574

Start: 3389459

End: 3390922

Strand: Reverse

Name: yhfT [H]

Synonym: BA_3688

Alternate gene names: 30263574

Gene position: 3390922-3389459 (Counterclockwise)

Preceding gene: 30263575

Following gene: 30263573

Centisome position: 64.87

GC content: 34.49

Gene sequence:

>1464_bases
ATGGGAATAACAAAAGAATATAAAAAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTTTT
AACATATAAAGAGTGGTTTGAGTCAGTGTATAAAGTAGCAAAATGGTTGAATGAAAAAGAATCGAAGAATAAAACGATAG
CGATTGTATTAGAAAATCGTATAGAGTTTTTACAACTATTCGCGGGTGCTGCTATGGCCGGATGGGTTTGTGTGCCACTA
GATATAAAGTGGAAACAAGATGAGCTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGACAGAGAGATATAA
GTTAAATGATTTACCGAATGAAGAAGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTA
CATATTTCCCTATAGAAAATGTACAAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGAAAAGCAAAAGCG
TTTTTACGTGCGCAACAATCGTGGCTTCATAGTTTTGATTGTAATGTACATGATTTTCATATGAAAAGAGAGGATTCGAT
TTTAATAGCTGGGACGCTTGTTCATTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGACAAACGGTTCACA
TTATGAGAAAGTTCATTCCAAATCAAGTATTAGATCAGTTAGAAACTGAAAATATATCAGTTATGTATACAGTTCCGACA
ATGCTTGAATCTCCATATAAAGAAAATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGC
TGGAGCGAAAGAAAAAATAAAGAATATCTTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTG
TAACAGCATTAGTTGATGAAGAGAGCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTA
TGTAATGAGGCAGGAAAAGAAGTACAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATA
CATAATAGATGGTGTTTTAGCACGGGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAG
AAGGATTTATTTATATTATCGGTAGAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAA
AGCGTATTACATGAACATCCAGCTGTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGT
CGCGATTGTAAAAGGAAGTGCTACGAGGCAACAATTAAAGCGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGA
AAGAATGGTATTTTGTAGATGAAATACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCAAAGAGTATCATTGAA
AATCAGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
CTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAAGAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTT
GTAAAGAAAGGACATCTCAT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACTATGAAGTTCAGCAATTAG
CAGCACCGCTTCTTACATTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPL
DIKWKQDELKERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKA
FLRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPT
MLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRV
CNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIE
SVLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
NQEKIYE

Sequences:

>Translated_487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPL
DIKWKQDELKERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKA
FLRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPT
MLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRV
CNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIE
SVLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
NQEKIYE
>Mature_486_residues
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLD
IKWKQDELKERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAF
LRAQQSWLHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTM
LESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVC
NEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIES
VLHEHPAVDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIEN
QEKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=374, Percent_Identity=27.807486631016, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=25, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=25, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI58082049, Length=471, Percent_Identity=23.9915074309979, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157311624, Length=481, Percent_Identity=24.7401247401247, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI157311622, Length=481, Percent_Identity=24.7401247401247, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=27.4647887323944, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=468, Percent_Identity=23.9316239316239, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI122937307, Length=375, Percent_Identity=24.2666666666667, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI38505220, Length=357, Percent_Identity=24.9299719887955, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI13375727, Length=427, Percent_Identity=21.0772833723653, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=514, Percent_Identity=24.3190661478599, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.0556660039761, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.9766081871345, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI221142682, Length=202, Percent_Identity=32.6732673267327, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=336, Percent_Identity=25.8928571428571, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790505, Length=424, Percent_Identity=21.9339622641509, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=25.0474383301708, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.9259259259259, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=23.8658777120316, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=301, Percent_Identity=30.5647840531561, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=28.099173553719, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=359, Percent_Identity=27.0194986072423, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=347, Percent_Identity=25.9365994236311, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=347, Percent_Identity=25.9365994236311, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=347, Percent_Identity=25.9365994236311, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.7617728531856, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=24.2424242424242, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=485, Percent_Identity=23.9175257731959, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=493, Percent_Identity=24.5436105476673, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=485, Percent_Identity=21.4432989690722, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=524, Percent_Identity=25, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=23.9786856127886, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=24.4855967078189, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=28.4457478005865, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=25.0501002004008, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21356947, Length=306, Percent_Identity=24.8366013071895, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=23.3400402414487, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356441, Length=463, Percent_Identity=23.110151187905, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.4852071005917, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648255, Length=507, Percent_Identity=22.2879684418146, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358303, Length=136, Percent_Identity=33.8235294117647, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=23.0337078651685, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161076582, Length=207, Percent_Identity=28.5024154589372, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=25.7281553398058, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=346, Percent_Identity=25.1445086705202, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667955, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648257, Length=499, Percent_Identity=20.8416833667335, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355225, Length=387, Percent_Identity=22.7390180878553, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56165; Mature: 56034

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENR
CCCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKLNDLPNEEGRVIE
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCEEE
IDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWLHSFDCNVHDFH
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
MLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDE
HHCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNAD
HHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHHHHHHHHHHCCCC
GWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVEDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHH
NQEKIYE
HHHHHCC
>Mature Secondary Structure 
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENR
CCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
IEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKLNDLPNEEGRVIE
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCEEE
IDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWLHSFDCNVHDFH
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
MLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDE
HHCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYIIDGVLARELNAD
HHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHHHHHHHHHHCCCC
GWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVEDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHH
NQEKIYE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]