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The map label for this gene is rspA [H]

Identifier: 21244946

GI number: 21244946

Start: 4991459

End: 4992718

Strand: Direct

Name: rspA [H]

Synonym: XAC4229

Alternate gene names: 21244946

Gene position: 4991459-4992718 (Clockwise)

Preceding gene: 21244945

Following gene: 77748767

Centisome position: 96.44

GC content: 65.16

Gene sequence:

>1260_bases
ATGTCACAGACTTCCGACTCCCCTGCACCGCTGCAAGGCTCTGCCCGCGACCGCGAGATCGTCGAGGCGCGCGTCATCGT
CACCTGCCCGGGGCGCAATTTCGTCACCTTGAAGATCCGCACGCGCTCGGGCATCACCGGTGTGGGCGATGCCACGCTCA
ACGGGCGCGAACTGGCGGTGGCCGCGTATCTGCAGGAACACCTGGTGCCGAATCTGATCGGCCGCGATGCCGGGCGCATC
GAAGACATCTGGCAGTTCTTCTACCGCGGCGCGTACTGGCGGCGCGGCCCGGTGACGATGAGCGCGATTGCCGCGGTGGA
TGTGGCGTTGTGGGACATCCTGGGCAAGATGGCCGGCATGCCGCTGTATCAGCTGCTCGGTGGGCGCTCACGCGAAGGTG
CGCTGGTGTACGGGCATGCCAACGGGCGCGATATCGCCGAAACCAGCGACGAAGTGGGCCGTTTCCGCGAAATGGGCTTT
ATCGCCATTCGCGCGCAATGCGGCGTGCCAGGCATCAAGAAGACCTACGGCATTTCTGTCGGCGGCAAGCCGTACGAACC
GGCCGAAAGCGAGCTGCCCACCGAAACCGTATGGTCCACGCCGCGCTACCTCGGCGTCGTGCCGCGCCTGTTCGAGCAAC
TGCGCGCCGACCATGGCGACGAGATCGAACTGCTGCACGATGCGCACCACCGGCTCACCCCGATCGAAGCCGCACGCCTG
GGCCGCGACCTGGAGCCGTACCGGCTGTTCTGGCTGGAAGATGCCACCCCGGCGGAAAACCAGCGTGCGTTCGAGATCAT
TCGCCAGCACACGGTGACGCCGCTGGCGGTGGGCGAGGTGTTCAATTCGATCTGGGACTGCAAGCACCTGATCGAGCAGC
AACTGATCGACTACATCCGCACCACGATCGTCCACGCCGGCGGTCTCACCCATGTGCGCCGGCTGGCCGATTTCGCCGCG
CTGCATCAGGTGCGCACCGGCTTCCACGGGGCCACCGACCTCTCGCCGGTGTGCATGGGCGCCGCCTTGCACTTCGACAC
CTGGGTACCCAATTTCGGCATCCAGGAATACATATTCCATTCCGACCAAGCCAATGCGGTATTCCCGCACGACTACCACT
TCCGCGATGGCCGCCTGCACTGCGGCGAGACGCCGGGGCATGGCGTGGACATCGACGAAGCGCTTGCAGCGAAGTATCCC
TACGTGCCCAAGCAGTTGCCGATCGCGCGACTGGAAGACGGCGCGATGTGGGATTGGTGA

Upstream 100 bases:

>100_bases
CCGACAGCCCTGTGTGCACCCTGTACGACCGCAGCGGCCTACCGGCCGGCCCGTTCGAATTTCCCATCACTGCCACCTCT
TCCCCCTGAGCGAGATCACG

Downstream 100 bases:

>100_bases
TGCGCGCGTTTTCTGCCACGTGTGTGGCCCTGCTGATGGCCTTGGCGGTGCCTGCGAACGCGCAGGCGCAGGCCTCTGCC
GATGTTGCGTTCGACTGGTT

Product: starvation sensing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSQTSDSPAPLQGSARDREIVEARVIVTCPGRNFVTLKIRTRSGITGVGDATLNGRELAVAAYLQEHLVPNLIGRDAGRI
EDIWQFFYRGAYWRRGPVTMSAIAAVDVALWDILGKMAGMPLYQLLGGRSREGALVYGHANGRDIAETSDEVGRFREMGF
IAIRAQCGVPGIKKTYGISVGGKPYEPAESELPTETVWSTPRYLGVVPRLFEQLRADHGDEIELLHDAHHRLTPIEAARL
GRDLEPYRLFWLEDATPAENQRAFEIIRQHTVTPLAVGEVFNSIWDCKHLIEQQLIDYIRTTIVHAGGLTHVRRLADFAA
LHQVRTGFHGATDLSPVCMGAALHFDTWVPNFGIQEYIFHSDQANAVFPHDYHFRDGRLHCGETPGHGVDIDEALAAKYP
YVPKQLPIARLEDGAMWDW

Sequences:

>Translated_419_residues
MSQTSDSPAPLQGSARDREIVEARVIVTCPGRNFVTLKIRTRSGITGVGDATLNGRELAVAAYLQEHLVPNLIGRDAGRI
EDIWQFFYRGAYWRRGPVTMSAIAAVDVALWDILGKMAGMPLYQLLGGRSREGALVYGHANGRDIAETSDEVGRFREMGF
IAIRAQCGVPGIKKTYGISVGGKPYEPAESELPTETVWSTPRYLGVVPRLFEQLRADHGDEIELLHDAHHRLTPIEAARL
GRDLEPYRLFWLEDATPAENQRAFEIIRQHTVTPLAVGEVFNSIWDCKHLIEQQLIDYIRTTIVHAGGLTHVRRLADFAA
LHQVRTGFHGATDLSPVCMGAALHFDTWVPNFGIQEYIFHSDQANAVFPHDYHFRDGRLHCGETPGHGVDIDEALAAKYP
YVPKQLPIARLEDGAMWDW
>Mature_418_residues
SQTSDSPAPLQGSARDREIVEARVIVTCPGRNFVTLKIRTRSGITGVGDATLNGRELAVAAYLQEHLVPNLIGRDAGRIE
DIWQFFYRGAYWRRGPVTMSAIAAVDVALWDILGKMAGMPLYQLLGGRSREGALVYGHANGRDIAETSDEVGRFREMGFI
AIRAQCGVPGIKKTYGISVGGKPYEPAESELPTETVWSTPRYLGVVPRLFEQLRADHGDEIELLHDAHHRLTPIEAARLG
RDLEPYRLFWLEDATPAENQRAFEIIRQHTVTPLAVGEVFNSIWDCKHLIEQQLIDYIRTTIVHAGGLTHVRRLADFAAL
HQVRTGFHGATDLSPVCMGAALHFDTWVPNFGIQEYIFHSDQANAVFPHDYHFRDGRLHCGETPGHGVDIDEALAAKYPY
VPKQLPIARLEDGAMWDW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=403, Percent_Identity=67.4937965260546, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI48994953, Length=396, Percent_Identity=30.3030303030303, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 46779; Mature: 46647

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSDSPAPLQGSARDREIVEARVIVTCPGRNFVTLKIRTRSGITGVGDATLNGRELAV
CCCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHH
AAYLQEHLVPNLIGRDAGRIEDIWQFFYRGAYWRRGPVTMSAIAAVDVALWDILGKMAGM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
PLYQLLGGRSREGALVYGHANGRDIAETSDEVGRFREMGFIAIRAQCGVPGIKKTYGISV
CHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHEEECC
GGKPYEPAESELPTETVWSTPRYLGVVPRLFEQLRADHGDEIELLHDAHHRLTPIEAARL
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHC
GRDLEPYRLFWLEDATPAENQRAFEIIRQHTVTPLAVGEVFNSIWDCKHLIEQQLIDYIR
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTIVHAGGLTHVRRLADFAALHQVRTGFHGATDLSPVCMGAALHFDTWVPNFGIQEYIFH
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHEE
SDQANAVFPHDYHFRDGRLHCGETPGHGVDIDEALAAKYPYVPKQLPIARLEDGAMWDW
CCCCCEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
SQTSDSPAPLQGSARDREIVEARVIVTCPGRNFVTLKIRTRSGITGVGDATLNGRELAV
CCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHH
AAYLQEHLVPNLIGRDAGRIEDIWQFFYRGAYWRRGPVTMSAIAAVDVALWDILGKMAGM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
PLYQLLGGRSREGALVYGHANGRDIAETSDEVGRFREMGFIAIRAQCGVPGIKKTYGISV
CHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHEEECC
GGKPYEPAESELPTETVWSTPRYLGVVPRLFEQLRADHGDEIELLHDAHHRLTPIEAARL
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHC
GRDLEPYRLFWLEDATPAENQRAFEIIRQHTVTPLAVGEVFNSIWDCKHLIEQQLIDYIR
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTIVHAGGLTHVRRLADFAALHQVRTGFHGATDLSPVCMGAALHFDTWVPNFGIQEYIFH
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHEE
SDQANAVFPHDYHFRDGRLHCGETPGHGVDIDEALAAKYPYVPKQLPIARLEDGAMWDW
CCCCCEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]