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The map label for this gene is leuD

Identifier: 21244182

GI number: 21244182

Start: 4079467

End: 4080114

Strand: Reverse

Name: leuD

Synonym: XAC3457

Alternate gene names: 21244182

Gene position: 4080114-4079467 (Counterclockwise)

Preceding gene: 21244183

Following gene: 21244181

Centisome position: 78.83

GC content: 61.27

Gene sequence:

>648_bases
ATGACTCCCTTCACCCGACACACCGGATTGGTCGCGCCGCTGGATCGCGCCAATGTCGATACCGACCAGATCATTCCCAA
GCAGTTCCTCAAGTCGATCAAGCGCACCGGTTTCGGTCCGAACCTGTTCGACGAGTGGCGCTATCTGGATATCGGCGAGC
CTGGTCGCGACAACAGCACGCGCCCGCTCAATCCGGAATTCGTGCTCAATTTCCCGCGCTATCAGGGCGCCAGCGTCTTG
CTGGCCCGGGAAAACTTCGGCTGTGGTTCCTCGCGCGAGCATGCGCCGTGGGCGCTGGACGAATACGGCTTTCGCACCGT
GATCGCGCCGAGCTTTGCCGACATTTTCTACAACAACAGTTTCAAGAACGGCCTGCTGCCGATCGTGCTGGCCGAAGCGC
AGGTCGATGCATTGTTCGAACAATGCCTGACGACCGAGGGCTACCAGCTCACCGTAGATCTGGCCGCGCAACGCGTACGC
CGCCCCGATGGCGTGGAGTACGGCTTCGACATCGATGCCTTCCGCAAACACTGCCTGCTCAATGGCCTGGACGATATCGG
CCTCACCTTGCAGGACGCCGATGCCATCGGGCGCTTCGAGCAAGGCCATCGCGCGCGCCAACCGTGGTTGTTTGGCGCGT
TGCAGTAA

Upstream 100 bases:

>100_bases
AATTGCAGGGAATCGAGAGTCGGGAATAGAGAATGGCAACGTCCGTCCCGTCTCCGCCCTGCCAATTCCCCATTCTCCAC
TCCCAATTCCCGGCATTCCA

Downstream 100 bases:

>100_bases
TGTCGAGCCGCAGATGCGCGCGCTCACCGCGCTGCGTGCGGACTGCATTGGATTGATGTCAATGACGCTGCGGCGTCACT
TCACGAGGGTGGCATGAGCA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTPFTRHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVL
LARENFGCGSSREHAPWALDEYGFRTVIAPSFADIFYNNSFKNGLLPIVLAEAQVDALFEQCLTTEGYQLTVDLAAQRVR
RPDGVEYGFDIDAFRKHCLLNGLDDIGLTLQDADAIGRFEQGHRARQPWLFGALQ

Sequences:

>Translated_215_residues
MTPFTRHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVL
LARENFGCGSSREHAPWALDEYGFRTVIAPSFADIFYNNSFKNGLLPIVLAEAQVDALFEQCLTTEGYQLTVDLAAQRVR
RPDGVEYGFDIDAFRKHCLLNGLDDIGLTLQDADAIGRFEQGHRARQPWLFGALQ
>Mature_214_residues
TPFTRHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVLL
ARENFGCGSSREHAPWALDEYGFRTVIAPSFADIFYNNSFKNGLLPIVLAEAQVDALFEQCLTTEGYQLTVDLAAQRVRR
PDGVEYGFDIDAFRKHCLLNGLDDIGLTLQDADAIGRFEQGHRARQPWLFGALQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=205, Percent_Identity=62.9268292682927, Blast_Score=254, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321429, Length=218, Percent_Identity=50.4587155963303, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_XANAC (Q8PH04)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643764.1
- ProteinModelPortal:   Q8PH04
- SMR:   Q8PH04
- GeneID:   1157528
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3457
- NMPDR:   fig|190486.1.peg.3408
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   XAXO190486:XAC3457-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24253; Mature: 24121

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFTRHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNST
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPEFVLNFPRYQGASVLLARENFGCGSSREHAPWALDEYGFRTVIAPSFADIFYNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHCCCC
FKNGLLPIVLAEAQVDALFEQCLTTEGYQLTVDLAAQRVRRPDGVEYGFDIDAFRKHCLL
CCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
NGLDDIGLTLQDADAIGRFEQGHRARQPWLFGALQ
CCCCCCCCEEECHHHHHHHHHCCCCCCCEEECCCC
>Mature Secondary Structure 
TPFTRHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNST
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPEFVLNFPRYQGASVLLARENFGCGSSREHAPWALDEYGFRTVIAPSFADIFYNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHCCCC
FKNGLLPIVLAEAQVDALFEQCLTTEGYQLTVDLAAQRVRRPDGVEYGFDIDAFRKHCLL
CCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
NGLDDIGLTLQDADAIGRFEQGHRARQPWLFGALQ
CCCCCCCCEEECHHHHHHHHHCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217