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The map label for this gene is leuB

Identifier: 21244181

GI number: 21244181

Start: 4078300

End: 4079373

Strand: Reverse

Name: leuB

Synonym: XAC3456

Alternate gene names: 21244181

Gene position: 4079373-4078300 (Counterclockwise)

Preceding gene: 21244182

Following gene: 21244175

Centisome position: 78.82

GC content: 62.57

Gene sequence:

>1074_bases
ATGAGCAAGCAGATTCTGATTCTTCCCGGCGACGGGATCGGGCCGGAGATCATGGCCGAAGCGGTCAAGGTGTTGAAGCG
CATTGACGCGCAACACGGTCTGGGTTTTCAGCTGGTGTATGACGAGCTGGGCGGCACTGCGTACGACAAATACGGCAGCC
CATTGGCCGATGAAACACTGGAGCGCGCGCGTGGCGCCGATGCGGTGCTGCTGGGTGCGGTGGGTGGCCCGCAGTGGGAC
ACTATCGACCCGGCGCTGCGGCCGGAGCGTGGGTTGCTCAAGATCCGCTCGCAGCTGGGCTTGTTCGCCAATCTGCGGCC
GGCATTGCTGTATCCGCAGCTGGCCGATGCGTCCACGCTCAAGCCGGAGGTCGTGGCCGGGCTGGATCTGCTGATCCTGC
GCGAACTCACGGGCGGCATCTATTTCGGCCAGCCGCGTGGTAACCGCACCTTGGACAACGGCGAACGCCAGGCGTACGAC
ACCTTGCCATACAGCGAGAGCGAGATTCGTCGCATCGCCAAGGCCGGCTTCGACATGGCACGCCTGCGCGGCAAGAAGCT
GTGTTCGGTCGACAAGGCCAACGTGCTGGCATCGAGCCAGCTATGGCGTGCAGTGGTCGAAGAAGTGGCCAAGGACTACC
CGGATATCGCGCTGTCGCATATGTACGTGGACAACGCAGCGATGCAGTTGGTGCGTGCTCCCAAGCAGTTCGACGTGATC
GTGACCGACAACATGTTCGGCGACATCCTGTCCGACCAGGCATCCATGCTCACCGGTTCCATCGGCATGCTGCCTTCGGC
CTCGCTGGATGCCAATAGCAAGGGTATGTACGAACCCTGCCATGGCTCGGCACCGGATATCGCCGGCAAGGGCATCGCCA
ATCCATTAGCCACGATCCTGTCGGTGGCGATGATGTTGCGCTACACCTTCGCGCGGGCCACTGCAGCAGATGCCATCGAA
CATGCGGTTGGCAAGGTGCTCGATCAAGGCCTGCGCACTGCCGACATCTGGTCGGAAGGCACTACCAAGGTCGGTACCGT
GGCGATGGGCGATGCGGTGGTGGCCGCGCTGTAA

Upstream 100 bases:

>100_bases
GCAGTAATGTCGAGCCGCAGATGCGCGCGCTCACCGCGCTGCGTGCGGACTGCATTGGATTGATGTCAATGACGCTGCGG
CGTCACTTCACGAGGGTGGC

Downstream 100 bases:

>100_bases
ACGCGCTCGCAGTCCCAAAGCGTTGTAGTACGCAGGAACGGTCGCCAGCGCGATAGCGCATCCGTGTCCTCAGCTGCCGA
CAGCAGCCGGCAGCAGCCGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFQLVYDELGGTAYDKYGSPLADETLERARGADAVLLGAVGGPQWD
TIDPALRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEIRRIAKAGFDMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFARATAADAIE
HAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Sequences:

>Translated_357_residues
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFQLVYDELGGTAYDKYGSPLADETLERARGADAVLLGAVGGPQWD
TIDPALRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEIRRIAKAGFDMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFARATAADAIE
HAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
>Mature_356_residues
SKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFQLVYDELGGTAYDKYGSPLADETLERARGADAVLLGAVGGPQWDT
IDPALRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDT
LPYSESEIRRIAKAGFDMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVIV
TDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFARATAADAIEH
AVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=308, Percent_Identity=33.4415584415584, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI28178816, Length=361, Percent_Identity=29.6398891966759, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4758582, Length=340, Percent_Identity=28.8235294117647, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178821, Length=309, Percent_Identity=30.42071197411, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.3577981651376, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=146, Percent_Identity=37.6712328767123, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=51.5406162464986, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=34.6478873239437, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=23.8805970149254, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=32.3699421965318, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=31.4606741573034, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=345, Percent_Identity=29.8550724637681, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=341, Percent_Identity=30.791788856305, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=49.8591549295775, Blast_Score=309, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=37.5350140056022, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=30.8781869688385, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=30.9248554913295, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=32.9341317365269, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=32.2485207100592, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24661184, Length=348, Percent_Identity=32.183908045977, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078633, Length=336, Percent_Identity=26.7857142857143, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24650122, Length=336, Percent_Identity=26.7857142857143, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=26.7857142857143, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=26.9461077844311, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI161078635, Length=336, Percent_Identity=26.7857142857143, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20130355, Length=341, Percent_Identity=25.5131964809384, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_XANAC (Q8PH05)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643763.1
- ProteinModelPortal:   Q8PH05
- SMR:   Q8PH05
- GeneID:   1157527
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3456
- NMPDR:   fig|190486.1.peg.3407
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- ProtClustDB:   PRK00772
- BioCyc:   XAXO190486:XAC3456-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38199; Mature: 38068

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFQLVYDELGGTAYDKYGSPLADETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCHHHCCCCHHHHHH
ERARGADAVLLGAVGGPQWDTIDPALRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEIRRIAKAGFDMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFARATAADAIEHAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC
>Mature Secondary Structure 
SKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFQLVYDELGGTAYDKYGSPLADETL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCHHHCCCCHHHHHH
ERARGADAVLLGAVGGPQWDTIDPALRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEIRRIAKAGFDMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFARATAADAIEHAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217