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The map label for this gene is thiC

Identifier: 21244172

GI number: 21244172

Start: 4062379

End: 4064256

Strand: Reverse

Name: thiC

Synonym: XAC3447

Alternate gene names: 21244172

Gene position: 4064256-4062379 (Counterclockwise)

Preceding gene: 21244174

Following gene: 21244171

Centisome position: 78.53

GC content: 63.1

Gene sequence:

>1878_bases
ATGAATGCCGCGCCCACCGTTTTGCAGCAACAAGCCCAGTCGTTGTCGGAGGCTGTCACCCAGCCGATTCCCGGTTCACG
CAAGATCTTCGTGCGAGGCTCGCGCGCGGATCTGCAAGTGCCGATGCGCGAGATCGCGTTGACGCGTACGCCCACCTTGT
TCGGTGGCGAAGAGAATCCGCCGCTCAGCGTCTACGACACGTCCGGGCCTTACACCGATCCGCGGGTGGCGATCGATCTG
GCAGCCGGTCTGGCACCGCTGCGTGCGCAGTGGATTGCCGAGCGTGGCGACACGCTCGCCCTGGACGGCCTGAGCTCCAG
CTTCGGGCGTGGCCGCGAGCACGATGCGCGTCTGGATGCAGTGCGCTTTCCGGCGCGGCGTCTGCCGCGGGTCGCGCGCC
AGGGCGCCAACGTCACCCAGATGCATTACGCGCGCCGCGGCATCATCACGCCGGAAATGGAGTACGTGGCGATCCGCGAA
AATCAGCGCCTGGAAGCGGTGACCGATGCAAGCTTGCGCAAGCAGCATCCGGGCCAGGCGTTCGGCGCCTCGATCCAGCA
ACGCATTACACCGGAGTTCGTGCGCGAAGAGATCGCACGCGGCCGTGCGATCTTGCCCAACAACATCAATCACCCCGAAA
GCGAGCCGATGATCATCGGCCGCAATTTCCTGACCAAGATCAATGCCAACATCGGCAACAGCGCGGTGTCCTCAGGCATT
GCCGAAGAAGTCGAGAAGCTGGTGTGGTCGATCCGCTGGGGCGGGGACACGGTCATGGATCTGTCCACCGGCAAGCACAT
CCATGAGACGCGCGAGTGGATCATCCGCAACTCGCCGGTGCCGATCGGCACGGTGCCGATCTATCAGGCATTGGAAAAGG
TCGATGGGCGCGCCGAAGAACTCACCTGGGACATCTTCCGCGACACCTTGATCGAACAGGCCGAACAGGGCGTGGATTAC
TTCACCATCCACGCTGGCGTGCTGCTGCGCTACGTGCCCCTGACCGCAAAGCGCGTGACCGGCATCGTGTCGCGTGGCGG
TTCGATCATGGCCAAGTGGTGCCTGGCGCATCACAAAGAGAACTTTCTCTACACGCACTTCGAAGACATCTGCCAGATCA
TGAAGGCCTACGACGTGGCGTTTTCGTTGGGCGATGGCCTACGCCCCGGCTGCATTGCCGATGCCAACGACGCGGCGCAA
TTCGGCGAGCTGGAAACGCTGGGCGAGCTGACCAAGCTCGCCTGGCAGCACGATGTGCAGACCATGATCGAAGGTCCCGG
CCATGTGCCGATGCAATTGATCAAGGAAAACATGGACAAGCAGCTGCGCGAATGCGGCGAGGCGCCGTTCTACACCCTGG
GCCCGCTGACCACCGACATCGCGCCGGGCTACGACCACATCACCAGCGCGATCGGCGCGGCAATGATCGGCTGGTTCGGC
ACCGCCATGCTCTGCTACGTCACTCCGAAAGAACACCTGGGCCTGCCCAACCGGCAGGACGTGCGCGACGGCATCATGGC
CTACAAGATCGCCGCGCATGCGGCCGACCTCGCCAAGGGGCATCCGGGCGCGCAGGTGCGCGACAACGCCTTGAGCAAGG
CGCGTTTCGAGTTTCGTTGGGACGACCAGTTCCATCTTGGCCTGGATCCTGAAAAGGCCAAGGAGTTCCACGATGAGACC
CTGCCCAAGGACGCGCACAAGCTGGCGCACTTCTGCTCGATGTGCGGGCCGCACTTCTGTTCGATGAAGATTACCCAGGA
CGTGCGTGATTACGCGAACGAGCACGGCGTGAGCGAGGCGCAAGCGCTATCGACAGGCATGCAGGAAAAGTCCGCGCAAT
TTGTCGCGCAGGGTGCACAGGTCTATCGCGCCACCTGA

Upstream 100 bases:

>100_bases
AGATGCGATGGTCCGTGTGTGCACGGGCGATCGGTATCGAGCGCCGCCGCTTCGTCCGCACTGCGACTCCTTCGCAGGGC
CAGCACACAGGACGAATGCC

Downstream 100 bases:

>100_bases
CGCGTGACATGGCAGCGCTTCACAGATCGTATGTAGCGCCTGCTTAGGAAAAATTCAGTTTTATATTGAACAGATTGCAG
CGCGGTTCGCAAGTGCCGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVRGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTLALDGLSSSFGRGREHDARLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEELTWDIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWQHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYANEHGVSEAQALSTGMQEKSAQFVAQGAQVYRAT

Sequences:

>Translated_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVRGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTLALDGLSSSFGRGREHDARLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEELTWDIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWQHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYANEHGVSEAQALSTGMQEKSAQFVAQGAQVYRAT
>Mature_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVRGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTLALDGLSSSFGRGREHDARLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEELTWDIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWQHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYANEHGVSEAQALSTGMQEKSAQFVAQGAQVYRAT

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=68.5337726523888, Blast_Score=887, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_XANAC (Q8PH13)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643754.1
- GeneID:   1157518
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3447
- NMPDR:   fig|190486.1.peg.3398
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   XAXO190486:XAC3447-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 69229; Mature: 69229

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 230-230 BINDING 259-259 BINDING 288-288 BINDING 324-324 BINDING 424-424 BINDING 451-451

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVRGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPRVAIDLAAGLAPLRAQWIAERGDTLALDGLSSSFGRGREHDARLDA
CEEEEECCCCCCCCCEEHHHHHCHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCHHHHH
VRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRKQHPGQA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEE
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHH
LTWDIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWQHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDETLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYANEHGVSEA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHH
QALSTGMQEKSAQFVAQGAQVYRAT
HHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVRGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPRVAIDLAAGLAPLRAQWIAERGDTLALDGLSSSFGRGREHDARLDA
CEEEEECCCCCCCCCEEHHHHHCHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCHHHHH
VRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRKQHPGQA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEE
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHH
LTWDIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWQHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDETLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYANEHGVSEA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHH
QALSTGMQEKSAQFVAQGAQVYRAT
HHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217