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The map label for this gene is metG [H]

Identifier: 21242139

GI number: 21242139

Start: 1592375

End: 1594459

Strand: Reverse

Name: metG [H]

Synonym: XAC1386

Alternate gene names: 21242139

Gene position: 1594459-1592375 (Counterclockwise)

Preceding gene: 21242140

Following gene: 21242138

Centisome position: 30.81

GC content: 65.28

Gene sequence:

>2085_bases
ATGACCCGCACCGCACTCGTCACCACTGCCCTGCCGTATGCCAACGGCCCGCTGCATCTCGGCCACTTGGTCGGCTACAT
CCAGGCCGATATCTGGGTGCGCGCGCGCCGGTTGCGCGGCGACAAGACCTGGTTCGTCTGTGCCGACGACACCCATGGCA
CGCCGATCATGCTGGCTGCCGAGAAGGCCGGGGTGACCCCGGAAGCCTTCATCGCCAACGTCCAGGCCAGCCACGAGCGC
GATTTCGCGGCGTTCGGCGTGACCTTCGACCACTACGATTCGACCAATTCGCCGGTCAATCGCGAGCTCACCGAAGCCTT
CTACGCCAAGCTGGAGGCCGCCGGCCATATCAGCCGGCGCTCGGTGGCGCAGTTCTACGATACGGCCAAGGGCATGTTCC
TGCCGGACCGCTACATCAAGGGCATCTGCCCCAACTGCGGCAGCCCCGACCAGTACGGCGACAACTGCGAAGTGTGCGGC
GCCACCTATGCGCCGACCGAGCTGAAGGAACCGAAGTCGGTGATTTCAGGCGCCACCCCGGAACTGCGCGATTCGGAGCA
TTTCTTCTTCGAAGTGGGCCATTTCGACGGATTTTTGCGCGAGTGGCTTGCCGGCGATGTGGCGCTTCCCGGGGTCAAGG
CCAAGCTCAAGGAATGGCTGGATGCCGAGGGCGGCCTGCGCGCCTGGGACATCTCGCGCGATGCGCCGTATTTCGGCTTC
CAGATTCCCGGCCAGCCGGGCAAGTATTTCTACGTGTGGCTGGATGCGCCGATCGGGTACCTGTGCAGCTTCAAGACGCT
GTGCGCGCAGATGGGCGAAAATTTCGAAGCGCATCTTGTCGCCGGCACCCAGACCGAGCTGCATCACTTCATCGGCAAGG
ACATCGTGAATTTCCACGGCCTGTTCTGGCCGGCCGTGCTGCACGGCACCGGCCACCGCGCGCCGACCCGCCTGCACGTC
AACGGCTACCTCACCGTGGACGGGGCCAAGATGTCCAAGTCGCGCGGCACCTTCGTGATGGCGCGGACCTTCCTGGATGT
CGGCCTGGAACCGGAAGCGCTGCGTTACTACTTCGCGGCCAAGTCCTCCGGCGGCGTGGACGACCTGGACCTCAACCTGG
GCGATTTCATCGCACGCGTGAATGCGGACCTGGTCGGCAAGTTCGTCAACCTGGCCAGCCGTTGCGCCGGCTTCATCGGC
AAGCGCTTCGATGGCAAACTGGCCGACGCCCTACCCGATGCCGCCCAGTACGACCGCTTCGTCGCCGCCCTGGCACCGAT
CCGCGAGGCCTACGAACGCAACGATGCCGCCAGCGCGATCCGCCAGACCATGGCGCTGGCCGACGAGGCCAATAAATACA
TCGACGACACCAAGCCGTGGGTGATCGCCAAGCAGGATGGCGCCGATGCGCAGCTGCAGTCGGTCTGCACGCAGGGCCTG
AACCTGTTCCGCATCCTGGTGGCCGCGCTCAAGCCGATCCTGCCGCGCACCTGCGCCGAAGCCGAAGCCTTCCTGTCGGC
GCCGATGACCAGTTGGGAAGACGTGATCGGCCCGCTGACGGCGCACACGATCCAGCCCTATACCGCCCTGTTTACCCGTA
TCGACCCCAAACTGATCGACGCCATGACCGACGCTTCCAAGGACACCCTCGCCGCACCTGCAACACCGGCTACCGCCTCC
AAGCCTGCACCGGCCAAGGCGGACGCAAAGCCCGCTGCAGCGGCGAATCCCCAATCCCCCATCGCCACTCCCGGCTTCAT
CGGCATGGACGATTTCGCCAAGCTCGACCTGCGCATCGGCAAGGTGCTGGCGTGCGAATTCGTGGAAGGCTCGGACAAGT
TGCTGCGCTTCGAACTGGACGCCGGCGAACTGGGCACGCGGCAGATCTTCTCCGGCATTCGCGCCAGCTATCGCGAACCG
GAAACGCTGGTCGGCCGCAGCGTGGTGTTCATTGCCAACCTGGCCCCGCGCAAGATGCGCTTTGGCATCAGCGAAGGCAT
GATCTTGTCGGCTGGCTTCGACGGCGGCGCCCTGGCGCTGCTGGACGCCGACAGCGGCGCACAGCCGGGCATGCCGGTCA
GGTGA

Upstream 100 bases:

>100_bases
ACCGGCCGCACGGCAACCGGCCGCTTCAACCAATTCCCGATTCCCGACTCCCTATTCCCGACTAGTGCGATAATCGCGTG
TCCCCCGCATTACGCCTGCC

Downstream 100 bases:

>100_bases
TGGGATTGGGAGTTGGGAATCGGGAATCGTAAAAGCGGAGTCTGCAAGGTCTGCTGATTGCGAGCTTGAGAATCGCCGCA
CCCGACATCCACACACCGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLRGDKTWFVCADDTHGTPIMLAAEKAGVTPEAFIANVQASHER
DFAAFGVTFDHYDSTNSPVNRELTEAFYAKLEAAGHISRRSVAQFYDTAKGMFLPDRYIKGICPNCGSPDQYGDNCEVCG
ATYAPTELKEPKSVISGATPELRDSEHFFFEVGHFDGFLREWLAGDVALPGVKAKLKEWLDAEGGLRAWDISRDAPYFGF
QIPGQPGKYFYVWLDAPIGYLCSFKTLCAQMGENFEAHLVAGTQTELHHFIGKDIVNFHGLFWPAVLHGTGHRAPTRLHV
NGYLTVDGAKMSKSRGTFVMARTFLDVGLEPEALRYYFAAKSSGGVDDLDLNLGDFIARVNADLVGKFVNLASRCAGFIG
KRFDGKLADALPDAAQYDRFVAALAPIREAYERNDAASAIRQTMALADEANKYIDDTKPWVIAKQDGADAQLQSVCTQGL
NLFRILVAALKPILPRTCAEAEAFLSAPMTSWEDVIGPLTAHTIQPYTALFTRIDPKLIDAMTDASKDTLAAPATPATAS
KPAPAKADAKPAAAANPQSPIATPGFIGMDDFAKLDLRIGKVLACEFVEGSDKLLRFELDAGELGTRQIFSGIRASYREP
ETLVGRSVVFIANLAPRKMRFGISEGMILSAGFDGGALALLDADSGAQPGMPVR

Sequences:

>Translated_694_residues
MTRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLRGDKTWFVCADDTHGTPIMLAAEKAGVTPEAFIANVQASHER
DFAAFGVTFDHYDSTNSPVNRELTEAFYAKLEAAGHISRRSVAQFYDTAKGMFLPDRYIKGICPNCGSPDQYGDNCEVCG
ATYAPTELKEPKSVISGATPELRDSEHFFFEVGHFDGFLREWLAGDVALPGVKAKLKEWLDAEGGLRAWDISRDAPYFGF
QIPGQPGKYFYVWLDAPIGYLCSFKTLCAQMGENFEAHLVAGTQTELHHFIGKDIVNFHGLFWPAVLHGTGHRAPTRLHV
NGYLTVDGAKMSKSRGTFVMARTFLDVGLEPEALRYYFAAKSSGGVDDLDLNLGDFIARVNADLVGKFVNLASRCAGFIG
KRFDGKLADALPDAAQYDRFVAALAPIREAYERNDAASAIRQTMALADEANKYIDDTKPWVIAKQDGADAQLQSVCTQGL
NLFRILVAALKPILPRTCAEAEAFLSAPMTSWEDVIGPLTAHTIQPYTALFTRIDPKLIDAMTDASKDTLAAPATPATAS
KPAPAKADAKPAAAANPQSPIATPGFIGMDDFAKLDLRIGKVLACEFVEGSDKLLRFELDAGELGTRQIFSGIRASYREP
ETLVGRSVVFIANLAPRKMRFGISEGMILSAGFDGGALALLDADSGAQPGMPVR
>Mature_693_residues
TRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLRGDKTWFVCADDTHGTPIMLAAEKAGVTPEAFIANVQASHERD
FAAFGVTFDHYDSTNSPVNRELTEAFYAKLEAAGHISRRSVAQFYDTAKGMFLPDRYIKGICPNCGSPDQYGDNCEVCGA
TYAPTELKEPKSVISGATPELRDSEHFFFEVGHFDGFLREWLAGDVALPGVKAKLKEWLDAEGGLRAWDISRDAPYFGFQ
IPGQPGKYFYVWLDAPIGYLCSFKTLCAQMGENFEAHLVAGTQTELHHFIGKDIVNFHGLFWPAVLHGTGHRAPTRLHVN
GYLTVDGAKMSKSRGTFVMARTFLDVGLEPEALRYYFAAKSSGGVDDLDLNLGDFIARVNADLVGKFVNLASRCAGFIGK
RFDGKLADALPDAAQYDRFVAALAPIREAYERNDAASAIRQTMALADEANKYIDDTKPWVIAKQDGADAQLQSVCTQGLN
LFRILVAALKPILPRTCAEAEAFLSAPMTSWEDVIGPLTAHTIQPYTALFTRIDPKLIDAMTDASKDTLAAPATPATASK
PAPAKADAKPAAAANPQSPIATPGFIGMDDFAKLDLRIGKVLACEFVEGSDKLLRFELDAGELGTRQIFSGIRASYREPE
TLVGRSVVFIANLAPRKMRFGISEGMILSAGFDGGALALLDADSGAQPGMPVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=589, Percent_Identity=27.5042444821732, Blast_Score=236, Evalue=6e-62,
Organism=Homo sapiens, GI24308436, Length=523, Percent_Identity=26.1950286806883, Blast_Score=153, Evalue=5e-37,
Organism=Escherichia coli, GI1788432, Length=699, Percent_Identity=53.79113018598, Blast_Score=779, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=620, Percent_Identity=27.741935483871, Blast_Score=228, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=517, Percent_Identity=23.7911025145068, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=23.4146341463415, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=28.673835125448, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6321610, Length=554, Percent_Identity=24.0072202166065, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24655311, Length=620, Percent_Identity=27.5806451612903, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI21358483, Length=527, Percent_Identity=26.7552182163188, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75338; Mature: 75207

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLRGDKTWFVCADDTHGTPIMLAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEE
EKAGVTPEAFIANVQASHERDFAAFGVTFDHYDSTNSPVNRELTEAFYAKLEAAGHISRR
CCCCCCHHHHHHHCCCCCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
SVAQFYDTAKGMFLPDRYIKGICPNCGSPDQYGDNCEVCGATYAPTELKEPKSVISGATP
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCC
ELRDSEHFFFEVGHFDGFLREWLAGDVALPGVKAKLKEWLDAEGGLRAWDISRDAPYFGF
CCCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEE
QIPGQPGKYFYVWLDAPIGYLCSFKTLCAQMGENFEAHLVAGTQTELHHFIGKDIVNFHG
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
LFWPAVLHGTGHRAPTRLHVNGYLTVDGAKMSKSRGTFVMARTFLDVGLEPEALRYYFAA
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEHHHHCCCCHHHHEEEEEE
KSSGGVDDLDLNLGDFIARVNADLVGKFVNLASRCAGFIGKRFDGKLADALPDAAQYDRF
CCCCCCCEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHH
VAALAPIREAYERNDAASAIRQTMALADEANKYIDDTKPWVIAKQDGADAQLQSVCTQGL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
NLFRILVAALKPILPRTCAEAEAFLSAPMTSWEDVIGPLTAHTIQPYTALFTRIDPKLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHCCHHHHHHHHCCHHHHH
AMTDASKDTLAAPATPATASKPAPAKADAKPAAAANPQSPIATPGFIGMDDFAKLDLRIG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
KVLACEFVEGSDKLLRFELDAGELGTRQIFSGIRASYREPETLVGRSVVFIANLAPRKMR
CEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHH
FGISEGMILSAGFDGGALALLDADSGAQPGMPVR
CCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TRTALVTTALPYANGPLHLGHLVGYIQADIWVRARRLRGDKTWFVCADDTHGTPIMLAA
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEE
EKAGVTPEAFIANVQASHERDFAAFGVTFDHYDSTNSPVNRELTEAFYAKLEAAGHISRR
CCCCCCHHHHHHHCCCCCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
SVAQFYDTAKGMFLPDRYIKGICPNCGSPDQYGDNCEVCGATYAPTELKEPKSVISGATP
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCC
ELRDSEHFFFEVGHFDGFLREWLAGDVALPGVKAKLKEWLDAEGGLRAWDISRDAPYFGF
CCCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEE
QIPGQPGKYFYVWLDAPIGYLCSFKTLCAQMGENFEAHLVAGTQTELHHFIGKDIVNFHG
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
LFWPAVLHGTGHRAPTRLHVNGYLTVDGAKMSKSRGTFVMARTFLDVGLEPEALRYYFAA
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEHHHHCCCCHHHHEEEEEE
KSSGGVDDLDLNLGDFIARVNADLVGKFVNLASRCAGFIGKRFDGKLADALPDAAQYDRF
CCCCCCCEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHH
VAALAPIREAYERNDAASAIRQTMALADEANKYIDDTKPWVIAKQDGADAQLQSVCTQGL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
NLFRILVAALKPILPRTCAEAEAFLSAPMTSWEDVIGPLTAHTIQPYTALFTRIDPKLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHCCHHHHHHHHCCHHHHH
AMTDASKDTLAAPATPATASKPAPAKADAKPAAAANPQSPIATPGFIGMDDFAKLDLRIG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
KVLACEFVEGSDKLLRFELDAGELGTRQIFSGIRASYREPETLVGRSVVFIANLAPRKMR
CEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHH
FGISEGMILSAGFDGGALALLDADSGAQPGMPVR
CCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA