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The map label for this gene is pleD [H]

Identifier: 21242098

GI number: 21242098

Start: 1551713

End: 1553422

Strand: Reverse

Name: pleD [H]

Synonym: XAC1345

Alternate gene names: 21242098

Gene position: 1553422-1551713 (Counterclockwise)

Preceding gene: 21242099

Following gene: 21242097

Centisome position: 30.01

GC content: 66.84

Gene sequence:

>1710_bases
ATGTCCTCTTCTTTTACGTCGCGCCGCCGCAACCAGCTGGCGCTGATTTTTTCTGCGCTGATCTTCGTCGTCATCGGAAT
CGGCATCTTCGTCGGTGCCCGCCGTTCGCTGGCCGATGCCGCGCTGGTGTCGCACACCCATGAGGTGATCGGGCGGGTCG
ACGAGATCCAGGCGCGCCTGCTCGATGCCGAGTCGGCGCAGCGCGGCTTCCTGCTCACCGGCAACGACGCCTACCTGCTG
GGCTACCAGACCAGTGTCGAACGTCTGCCGATCCTGCTGGACAACCTGCGCCGCCTGATCGTCGACAACCCCGCACAGGA
GCAGCACCTGGCGCAGTTGCAACGGCTGGTGGAAACCCGCCTGCGGCAGATTCAGCACGTGCTCGACATTTATACGCAAA
GTGGGCTGGAGCCGGCACGTGCCGACATCCGGCAGAGCGCCTTCCGCACCACCAGCGCGATCCGCGAGCTGGCATTGGCG
ATGGTGCAGCGCGAACAGGAGCTGCTGGCACAGCGCGCGCAAAGCAGCGAACAAAGCGCCAACCTGTTGCTTGCGCTGGC
ACTGGCCGGCATTCCGTTCGGGCTGGTGGTGGTGGGCATGGTCTATGCGCTGTTGATGCGCGAATTGCGCCACCGCGCGC
AGGCCGAACAGCTGGCGGCGCAGGCCAATCGCGAGTTGGGCCAGAGCATCGACGCGTTGCAGCGCAGTACCGCCGATCTC
AACCTGCTCAGCCGTTACACCGGGCTGCTGCAGAGCTGCATCAGTGCCGAAGAAGCGTTGATGGTCAGCAGCCGTACCCT
GGCCAGCCTGCTGCCGGGCGTGGCGGGAAGCGTGTACCTGCTGCGCGCCTCGCAAGACCGCGCCGAGGCGATCAGCCACT
GGGGCGAGCCGCTGGTGCGCAGCGCGCCGCATCTGTCGCCGGAGCAGTGCTGGGCGCTGCGCCGCGGCCAGCCGCACATC
GTCCAAGACCTGGGTCGGGATGCACTGTGTGCGCATATCGAAGAACCCGCACACGATGCGTCGGTCACCACCGCCTGCCT
GCCGATGTCGGCGCAAGGCACCCAGCTGGGGTTTCTGTTCCTGTCCGCAGCCGGCCCCGGGCCGATGCCGCGGCTGGAAA
TCGCCGAGGCGGCCGCCGAGCAGTTGTCGTTGGCGCTGAGCAACCTGCGCCTGCGCGAATCGCTGCGCCGGCAGTCGATC
CGCGATGCGCTGACGGGGCTGTATAACCGCCGCTATCTGGAAGAATCGCTGAGCCACGAGTTGGCGCGCTGCGCACGGCG
TGGGTTGCCGTTGCCGGTCTTGATGCTGGACGTGGACCATTTCAAGCAGTTCAACGACAGCCAGGGCCATGCCGGTGGCG
ACCTGTTGCTGGCAGCGGTGGGCGAATTGTTGCTGACCCGCCTGCGCGCCGAGGACGTGGCCTGTCGCTACGGCGGCGAG
GAATTCACCGTCCTTCTGCCCGAAGCCGATGGCGAAGAAGCCATGCGCGTGGCCGAGCAGATCCGCGGTTACATCGCGGC
ACTGGCGGTCAGCGACGGTGCGCGCGCCTTGCCGAAGGTGACCGCCTCGATCGGCGTGGCCAGCTTCCCGGTCGATGGCG
AACAGGGTGCGAGCCTGATCCAGAAAGCCGACGCTGCGTTGTATCGCGCCAAGCACGCGGGGCGCAACCGGGTGGAGCGA
CACGGTGCTGCGACGGACAATGCGGACTGA

Upstream 100 bases:

>100_bases
CCCTTTCGCAGCACCCGCAACGCGCCGGCCATGTGCCGGCGCGGTTGTCTCGGCCGACGGATGGCTCGTGTTGTCGAGGC
CGGCAGGCTAGCATTCGCAT

Downstream 100 bases:

>100_bases
GAGCAGCGACCAAGACCACCGCGCGGCAGCCAGCCAGTCGCGCCCGCGCCCGCGCCCGCGCCCGAAACCGGGATGTAGCA
CTCAAGACCGCGGCCCTGGT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSSSFTSRRRNQLALIFSALIFVVIGIGIFVGARRSLADAALVSHTHEVIGRVDEIQARLLDAESAQRGFLLTGNDAYLL
GYQTSVERLPILLDNLRRLIVDNPAQEQHLAQLQRLVETRLRQIQHVLDIYTQSGLEPARADIRQSAFRTTSAIRELALA
MVQREQELLAQRAQSSEQSANLLLALALAGIPFGLVVVGMVYALLMRELRHRAQAEQLAAQANRELGQSIDALQRSTADL
NLLSRYTGLLQSCISAEEALMVSSRTLASLLPGVAGSVYLLRASQDRAEAISHWGEPLVRSAPHLSPEQCWALRRGQPHI
VQDLGRDALCAHIEEPAHDASVTTACLPMSAQGTQLGFLFLSAAGPGPMPRLEIAEAAAEQLSLALSNLRLRESLRRQSI
RDALTGLYNRRYLEESLSHELARCARRGLPLPVLMLDVDHFKQFNDSQGHAGGDLLLAAVGELLLTRLRAEDVACRYGGE
EFTVLLPEADGEEAMRVAEQIRGYIAALAVSDGARALPKVTASIGVASFPVDGEQGASLIQKADAALYRAKHAGRNRVER
HGAATDNAD

Sequences:

>Translated_569_residues
MSSSFTSRRRNQLALIFSALIFVVIGIGIFVGARRSLADAALVSHTHEVIGRVDEIQARLLDAESAQRGFLLTGNDAYLL
GYQTSVERLPILLDNLRRLIVDNPAQEQHLAQLQRLVETRLRQIQHVLDIYTQSGLEPARADIRQSAFRTTSAIRELALA
MVQREQELLAQRAQSSEQSANLLLALALAGIPFGLVVVGMVYALLMRELRHRAQAEQLAAQANRELGQSIDALQRSTADL
NLLSRYTGLLQSCISAEEALMVSSRTLASLLPGVAGSVYLLRASQDRAEAISHWGEPLVRSAPHLSPEQCWALRRGQPHI
VQDLGRDALCAHIEEPAHDASVTTACLPMSAQGTQLGFLFLSAAGPGPMPRLEIAEAAAEQLSLALSNLRLRESLRRQSI
RDALTGLYNRRYLEESLSHELARCARRGLPLPVLMLDVDHFKQFNDSQGHAGGDLLLAAVGELLLTRLRAEDVACRYGGE
EFTVLLPEADGEEAMRVAEQIRGYIAALAVSDGARALPKVTASIGVASFPVDGEQGASLIQKADAALYRAKHAGRNRVER
HGAATDNAD
>Mature_568_residues
SSSFTSRRRNQLALIFSALIFVVIGIGIFVGARRSLADAALVSHTHEVIGRVDEIQARLLDAESAQRGFLLTGNDAYLLG
YQTSVERLPILLDNLRRLIVDNPAQEQHLAQLQRLVETRLRQIQHVLDIYTQSGLEPARADIRQSAFRTTSAIRELALAM
VQREQELLAQRAQSSEQSANLLLALALAGIPFGLVVVGMVYALLMRELRHRAQAEQLAAQANRELGQSIDALQRSTADLN
LLSRYTGLLQSCISAEEALMVSSRTLASLLPGVAGSVYLLRASQDRAEAISHWGEPLVRSAPHLSPEQCWALRRGQPHIV
QDLGRDALCAHIEEPAHDASVTTACLPMSAQGTQLGFLFLSAAGPGPMPRLEIAEAAAEQLSLALSNLRLRESLRRQSIR
DALTGLYNRRYLEESLSHELARCARRGLPLPVLMLDVDHFKQFNDSQGHAGGDLLLAAVGELLLTRLRAEDVACRYGGEE
FTVLLPEADGEEAMRVAEQIRGYIAALAVSDGARALPKVTASIGVASFPVDGEQGASLIQKADAALYRAKHAGRNRVERH
GAATDNAD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=42.0731707317073, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=41.6216216216216, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=37.7777777777778, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=38.3647798742138, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=40.4624277456647, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.6459627329193, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=36.4705882352941, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787802, Length=216, Percent_Identity=30.0925925925926, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=30.1204819277108, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI87081974, Length=120, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=33.1395348837209, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=33.1360946745562, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61979; Mature: 61847

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSFTSRRRNQLALIFSALIFVVIGIGIFVGARRSLADAALVSHTHEVIGRVDEIQARL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAESAQRGFLLTGNDAYLLGYQTSVERLPILLDNLRRLIVDNPAQEQHLAQLQRLVETR
HHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRQIQHVLDIYTQSGLEPARADIRQSAFRTTSAIRELALAMVQREQELLAQRAQSSEQSA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NLLLALALAGIPFGLVVVGMVYALLMRELRHRAQAEQLAAQANRELGQSIDALQRSTADL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLSRYTGLLQSCISAEEALMVSSRTLASLLPGVAGSVYLLRASQDRAEAISHWGEPLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHH
SAPHLSPEQCWALRRGQPHIVQDLGRDALCAHIEEPAHDASVTTACLPMSAQGTQLGFLF
CCCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEE
LSAAGPGPMPRLEIAEAAAEQLSLALSNLRLRESLRRQSIRDALTGLYNRRYLEESLSHE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARCARRGLPLPVLMLDVDHFKQFNDSQGHAGGDLLLAAVGELLLTRLRAEDVACRYGGE
HHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFTVLLPEADGEEAMRVAEQIRGYIAALAVSDGARALPKVTASIGVASFPVDGEQGASLI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCHHHHHHH
QKADAALYRAKHAGRNRVERHGAATDNAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSSFTSRRRNQLALIFSALIFVVIGIGIFVGARRSLADAALVSHTHEVIGRVDEIQARL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAESAQRGFLLTGNDAYLLGYQTSVERLPILLDNLRRLIVDNPAQEQHLAQLQRLVETR
HHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRQIQHVLDIYTQSGLEPARADIRQSAFRTTSAIRELALAMVQREQELLAQRAQSSEQSA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NLLLALALAGIPFGLVVVGMVYALLMRELRHRAQAEQLAAQANRELGQSIDALQRSTADL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLSRYTGLLQSCISAEEALMVSSRTLASLLPGVAGSVYLLRASQDRAEAISHWGEPLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHH
SAPHLSPEQCWALRRGQPHIVQDLGRDALCAHIEEPAHDASVTTACLPMSAQGTQLGFLF
CCCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEE
LSAAGPGPMPRLEIAEAAAEQLSLALSNLRLRESLRRQSIRDALTGLYNRRYLEESLSHE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARCARRGLPLPVLMLDVDHFKQFNDSQGHAGGDLLLAAVGELLLTRLRAEDVACRYGGE
HHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFTVLLPEADGEEAMRVAEQIRGYIAALAVSDGARALPKVTASIGVASFPVDGEQGASLI
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCHHHHHHH
QKADAALYRAKHAGRNRVERHGAATDNAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA