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The map label for this gene is dosC [H]

Identifier: 21241032

GI number: 21241032

Start: 308105

End: 309163

Strand: Direct

Name: dosC [H]

Synonym: XAC0258

Alternate gene names: 21241032

Gene position: 308105-309163 (Clockwise)

Preceding gene: 21241031

Following gene: 21241033

Centisome position: 5.95

GC content: 65.72

Gene sequence:

>1059_bases
ATGACTTGCCACAACGCCGCAGGCGCACTTGAGCCGACGGGGAGCGCTGCCAAAACGCTCCCCGACGGGCTGCATGCGCT
GATGACTGCCGAGGAACTGGCGTTTTTCGCCCGTTTCGGCCGTTTACGCGAGGTGTCCGCGGGCCAGTCGCTGTTCGAGC
GCGGTGCCGTAGGCACGCAGATGTACATCGTTGTCAGCGGCCAGATCGACCTGGATTTCGGTGAGGACCTGATGCTCAAG
CATCTGGGCCCGGGAGAATTCTTCGGCGAGCTCGGCCTGCTGATCGGCGATCACGCGCGCAGCGCCGGGGCCAGCGCCTC
CACCGACAGCCGTCTGATCGAGCTGGCACACGACGATTTCCAGCGCCTGGTCGACCAGGATCCCTCGATGGTGGCGCACT
TCCTGCGCCGCTCCATCGTGCGGGTGGTCAACAACGAACAATTGCTGATCCGCCAGCTGCGCCGCCGCAATCACGATCTG
GAAGCGGCGCTGGACAACCTGTACGTCACCTCGCACCAGCTCAACCACACCGAAGAACTGAGCCGTACCGACGAGCTCAC
CGGCTTGCACAACCGGCGCGGCCTGGCGTTGCACCTGCAGGAATGCCGGCGGGCCGGTCACGTGCCGGGCGTCGGCCTGA
TCCTGATCGACTGCGACCGCTTCAAGCGCATCAACGACGAGTTCGGTCATCTGGCAGGCGACCGCGTGCTGCAGAACGTG
GCGCATGCCCTGCGTTCGGTGATCGCCGAAGGCGACTTGGCCTGTCGTCTCGGCGGCGACGAATTCTGCGTGCTCGTCGC
ACAAGGCACCAGCGAGCTGGTGCACCACATCGGCGAATGCATCGTGCGGGCGGTGGAAGCGCGGCTGTGCAACGGCCAGG
GCGAGCAAGGCTGCTCGGTCAGCGTGGGCTCGTGCATGATCGATGCGGACGCGGCGTGGAACGACTGGTACACGCTCGCC
GACAGCGCCTTGTACGAGGCCAAGCGGCAGGGCGGCAATGTGCTGTGCATGCAGGAGACGCTGGCCCTGGATCAACCGCC
TGCATCGGGTCACCGCTGA

Upstream 100 bases:

>100_bases
CTCTTTCTTCATGAGAAGGAGTGTGACCTGGTGCTGAACAAGGGAGTCCTGTAGGATTTGTCCATGGGCTGACGTTGCAG
GCCGGCCTAAGGGAAAACCG

Downstream 100 bases:

>100_bases
GCGATGGGCAGCCGCACCCACGCACGCAAGGACGCGTCGTCGGAAGCGCTCCAGGCGCCGCGGCTGCGCACCTTGTTGTT
GACCGATCTCTGCGATTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTCHNAAGALEPTGSAAKTLPDGLHALMTAEELAFFARFGRLREVSAGQSLFERGAVGTQMYIVVSGQIDLDFGEDLMLK
HLGPGEFFGELGLLIGDHARSAGASASTDSRLIELAHDDFQRLVDQDPSMVAHFLRRSIVRVVNNEQLLIRQLRRRNHDL
EAALDNLYVTSHQLNHTEELSRTDELTGLHNRRGLALHLQECRRAGHVPGVGLILIDCDRFKRINDEFGHLAGDRVLQNV
AHALRSVIAEGDLACRLGGDEFCVLVAQGTSELVHHIGECIVRAVEARLCNGQGEQGCSVSVGSCMIDADAAWNDWYTLA
DSALYEAKRQGGNVLCMQETLALDQPPASGHR

Sequences:

>Translated_352_residues
MTCHNAAGALEPTGSAAKTLPDGLHALMTAEELAFFARFGRLREVSAGQSLFERGAVGTQMYIVVSGQIDLDFGEDLMLK
HLGPGEFFGELGLLIGDHARSAGASASTDSRLIELAHDDFQRLVDQDPSMVAHFLRRSIVRVVNNEQLLIRQLRRRNHDL
EAALDNLYVTSHQLNHTEELSRTDELTGLHNRRGLALHLQECRRAGHVPGVGLILIDCDRFKRINDEFGHLAGDRVLQNV
AHALRSVIAEGDLACRLGGDEFCVLVAQGTSELVHHIGECIVRAVEARLCNGQGEQGCSVSVGSCMIDADAAWNDWYTLA
DSALYEAKRQGGNVLCMQETLALDQPPASGHR
>Mature_351_residues
TCHNAAGALEPTGSAAKTLPDGLHALMTAEELAFFARFGRLREVSAGQSLFERGAVGTQMYIVVSGQIDLDFGEDLMLKH
LGPGEFFGELGLLIGDHARSAGASASTDSRLIELAHDDFQRLVDQDPSMVAHFLRRSIVRVVNNEQLLIRQLRRRNHDLE
AALDNLYVTSHQLNHTEELSRTDELTGLHNRRGLALHLQECRRAGHVPGVGLILIDCDRFKRINDEFGHLAGDRVLQNVA
HALRSVIAEGDLACRLGGDEFCVLVAQGTSELVHHIGECIVRAVEARLCNGQGEQGCSVSVGSCMIDADAAWNDWYTLAD
SALYEAKRQGGNVLCMQETLALDQPPASGHR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=284, Percent_Identity=30.2816901408451, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081881, Length=153, Percent_Identity=35.2941176470588, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=30.6748466257669, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=32.1212121212121, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082007, Length=176, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=29.4797687861272, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=31.0126582278481, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=33.9622641509434, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=204, Percent_Identity=30.3921568627451, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=221, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788381, Length=74, Percent_Identity=41.8918918918919, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38432; Mature: 38301

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCHNAAGALEPTGSAAKTLPDGLHALMTAEELAFFARFGRLREVSAGQSLFERGAVGTQ
CCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
MYIVVSGQIDLDFGEDLMLKHLGPGEFFGELGLLIGDHARSAGASASTDSRLIELAHDDF
EEEEEECEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
QRLVDQDPSMVAHFLRRSIVRVVNNEQLLIRQLRRRNHDLEAALDNLYVTSHQLNHTEEL
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHEEEHHHCCCHHHH
SRTDELTGLHNRRGLALHLQECRRAGHVPGVGLILIDCDRFKRINDEFGHLAGDRVLQNV
HHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AHALRSVIAEGDLACRLGGDEFCVLVAQGTSELVHHIGECIVRAVEARLCNGQGEQGCSV
HHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
SVGSCMIDADAAWNDWYTLADSALYEAKRQGGNVLCMQETLALDQPPASGHR
ECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TCHNAAGALEPTGSAAKTLPDGLHALMTAEELAFFARFGRLREVSAGQSLFERGAVGTQ
CCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
MYIVVSGQIDLDFGEDLMLKHLGPGEFFGELGLLIGDHARSAGASASTDSRLIELAHDDF
EEEEEECEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
QRLVDQDPSMVAHFLRRSIVRVVNNEQLLIRQLRRRNHDLEAALDNLYVTSHQLNHTEEL
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHEEEHHHCCCHHHH
SRTDELTGLHNRRGLALHLQECRRAGHVPGVGLILIDCDRFKRINDEFGHLAGDRVLQNV
HHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AHALRSVIAEGDLACRLGGDEFCVLVAQGTSELVHHIGECIVRAVEARLCNGQGEQGCSV
HHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
SVGSCMIDADAAWNDWYTLADSALYEAKRQGGNVLCMQETLALDQPPASGHR
ECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]