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The map label for this gene is aceA [H]

Identifier: 21241031

GI number: 21241031

Start: 306512

End: 307807

Strand: Direct

Name: aceA [H]

Synonym: XAC0257

Alternate gene names: 21241031

Gene position: 306512-307807 (Clockwise)

Preceding gene: 21241030

Following gene: 21241032

Centisome position: 5.92

GC content: 61.57

Gene sequence:

>1296_bases
ATGAGCACTACACTGCAAAGCGCCGAACAACTGCAGCAGGACTGGTCCAACAACCCTCGCTGGGCCGGCATCGCCCGCAA
TTACAGCGCTGCCGATGTGGCGCGCCTGCGTGGCACCGTGCATGTCGAGCATTCGCTGGCACGCCTGGGTGCAGAAAAAC
TGTGGAATTCCCTGCATGCCACACCGTTCGTCAATGCGCTGGGCGCGCTGACCGGCAACCAGGCCATGCAACAGATCAAG
GCCGGCCTGAAGGCGATTTACCTGAGCGGTTGGCAGGTTGCCGCCGATGCCAATCTGGCCGGGCAGATGTATCCGGATCA
ATCGCTATACCCGGCCGATTCCGTGCCGGCGGTGGTCAAGCGCATCAACAATACCTTGCTGCGCGCCGACCAGCTCCATC
ATGCCGAAGGCAACGATGCCATCGACTTCCTGCAGCCGATCGTGGCCGACGCCGAAGCCGGTTTCGGCGGCGTGCTCAAC
GCCTTCGAACTGATGAAGGCGATGATCGAAGCCGGTGCGGCCGGTGTGCACTTCGAAGACCAGCTGGCCTCGGTCAAGAA
ATGCGGCCATATGGGCGGCAAGGTGCTGGTGCCCACGCGTGAGGCGATCGAAAAATTGAATGCCGCGCGCCTGGCCGCCG
ATGTGCTGGGCGTGCCGACGGTGCTGATCGCGCGCACAGACGCCGAAGCCGCCGACCTGATCACCAGCGATGTGGATGCC
AACGATCAACCGTTCACCACCGGCGAGCGTACGGTCGAAGGCTTCTTCAAGACCCGCAATGGCCTGGACCAGGCAATCAG
CCGCGGCTTGGCCTATGCCCCGTACGCCGATCTGATCTGGTGCGAAACGGGCAAGCCGGACCTGGAATTTGCGCGCGCCT
TCGCACAGGCCATCCATGCCAGGTTCCCCGGCAAATTGCTGGCCTACAACTGCTCGCCGAGTTTCAACTGGAAGAAGAAT
CTGGACGACGCCACCATTGCCAAATTCCAGACCGAACTGGCGAGCTATGGCTACAAGTTCCAGTTCATCACCCTGGCCGG
TTTCCATGCGTTGAATCACAGCATGTTCCATCTGGCGCACGGCTATGCGCGCCGGCAGATGAGCGCATTCGTCGAATTGC
AGCAGGCCGAGTTCGAGGCGGCGGAGATGGGATTTACCGCAGTCAAGCATCAGCGCGAGGTGGGCACCGGCTATTTCGAT
GCAGTGACGCAGGCGATCCAGCAGGGGCAATCGTCCACGACCGCGTTGCGTGGCTCCACTGAAGAAGAGCAATTCCAAAG
CGACAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCACGCACGCGCAACGACGGCCTCGCCATGCCGCCTGCCTTGGCATGCCCGCCATTCCTTCCTCGTTCCATCCGTCGT
ACTTCTCAGGAGAACGCAAG

Downstream 100 bases:

>100_bases
TTGCTGGCAGCACTGCAACGTCGGCGGATGTACATGAGGCAATGCAGTGACGCAGCACCTGGACGCTCAGTTGATGCAGC
TGTCTCCATGGCAATGGACG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTTLQSAEQLQQDWSNNPRWAGIARNYSAADVARLRGTVHVEHSLARLGAEKLWNSLHATPFVNALGALTGNQAMQQIK
AGLKAIYLSGWQVAADANLAGQMYPDQSLYPADSVPAVVKRINNTLLRADQLHHAEGNDAIDFLQPIVADAEAGFGGVLN
AFELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAIEKLNAARLAADVLGVPTVLIARTDAEAADLITSDVDA
NDQPFTTGERTVEGFFKTRNGLDQAISRGLAYAPYADLIWCETGKPDLEFARAFAQAIHARFPGKLLAYNCSPSFNWKKN
LDDATIAKFQTELASYGYKFQFITLAGFHALNHSMFHLAHGYARRQMSAFVELQQAEFEAAEMGFTAVKHQREVGTGYFD
AVTQAIQQGQSSTTALRGSTEEEQFQSDKAA

Sequences:

>Translated_431_residues
MSTTLQSAEQLQQDWSNNPRWAGIARNYSAADVARLRGTVHVEHSLARLGAEKLWNSLHATPFVNALGALTGNQAMQQIK
AGLKAIYLSGWQVAADANLAGQMYPDQSLYPADSVPAVVKRINNTLLRADQLHHAEGNDAIDFLQPIVADAEAGFGGVLN
AFELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAIEKLNAARLAADVLGVPTVLIARTDAEAADLITSDVDA
NDQPFTTGERTVEGFFKTRNGLDQAISRGLAYAPYADLIWCETGKPDLEFARAFAQAIHARFPGKLLAYNCSPSFNWKKN
LDDATIAKFQTELASYGYKFQFITLAGFHALNHSMFHLAHGYARRQMSAFVELQQAEFEAAEMGFTAVKHQREVGTGYFD
AVTQAIQQGQSSTTALRGSTEEEQFQSDKAA
>Mature_430_residues
STTLQSAEQLQQDWSNNPRWAGIARNYSAADVARLRGTVHVEHSLARLGAEKLWNSLHATPFVNALGALTGNQAMQQIKA
GLKAIYLSGWQVAADANLAGQMYPDQSLYPADSVPAVVKRINNTLLRADQLHHAEGNDAIDFLQPIVADAEAGFGGVLNA
FELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAIEKLNAARLAADVLGVPTVLIARTDAEAADLITSDVDAN
DQPFTTGERTVEGFFKTRNGLDQAISRGLAYAPYADLIWCETGKPDLEFARAFAQAIHARFPGKLLAYNCSPSFNWKKNL
DDATIAKFQTELASYGYKFQFITLAGFHALNHSMFHLAHGYARRQMSAFVELQQAEFEAAEMGFTAVKHQREVGTGYFDA
VTQAIQQGQSSTTALRGSTEEEQFQSDKAA

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=69.1954022988506, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1786525, Length=296, Percent_Identity=28.0405405405405, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=415, Percent_Identity=60.7228915662651, Blast_Score=526, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71982926, Length=415, Percent_Identity=60.7228915662651, Blast_Score=526, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6320908, Length=289, Percent_Identity=37.3702422145329, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325263, Length=255, Percent_Identity=35.6862745098039, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 46855; Mature: 46724

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLQSAEQLQQDWSNNPRWAGIARNYSAADVARLRGTVHVEHSLARLGAEKLWNSLHA
CCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
TPFVNALGALTGNQAMQQIKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPADSVPAVVK
CHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
RINNTLLRADQLHHAEGNDAIDFLQPIVADAEAGFGGVLNAFELMKAMIEAGAAGVHFED
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
QLASVKKCGHMGGKVLVPTREAIEKLNAARLAADVLGVPTVLIARTDAEAADLITSDVDA
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCC
NDQPFTTGERTVEGFFKTRNGLDQAISRGLAYAPYADLIWCETGKPDLEFARAFAQAIHA
CCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RFPGKLLAYNCSPSFNWKKNLDDATIAKFQTELASYGYKFQFITLAGFHALNHSMFHLAH
HCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHH
GYARRQMSAFVELQQAEFEAAEMGFTAVKHQREVGTGYFDAVTQAIQQGQSSTTALRGST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHCCCC
EEEQFQSDKAA
HHHHHHHCCCC
>Mature Secondary Structure 
STTLQSAEQLQQDWSNNPRWAGIARNYSAADVARLRGTVHVEHSLARLGAEKLWNSLHA
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
TPFVNALGALTGNQAMQQIKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPADSVPAVVK
CHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
RINNTLLRADQLHHAEGNDAIDFLQPIVADAEAGFGGVLNAFELMKAMIEAGAAGVHFED
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
QLASVKKCGHMGGKVLVPTREAIEKLNAARLAADVLGVPTVLIARTDAEAADLITSDVDA
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCC
NDQPFTTGERTVEGFFKTRNGLDQAISRGLAYAPYADLIWCETGKPDLEFARAFAQAIHA
CCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RFPGKLLAYNCSPSFNWKKNLDDATIAKFQTELASYGYKFQFITLAGFHALNHSMFHLAH
HCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHH
GYARRQMSAFVELQQAEFEAAEMGFTAVKHQREVGTGYFDAVTQAIQQGQSSTTALRGST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHCCCC
EEEQFQSDKAA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]