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The map label for this gene is amiA [H]

Identifier: 57238141

GI number: 57238141

Start: 1320896

End: 1322875

Strand: Reverse

Name: amiA [H]

Synonym: CJE1405

Alternate gene names: 57238141

Gene position: 1322875-1320896 (Counterclockwise)

Preceding gene: 57238142

Following gene: 57238140

Centisome position: 74.41

GC content: 29.14

Gene sequence:

>1980_bases
ATGGCTAAAATTTTTGTATTTTTAGTATTTGCTTTCACAACTCTTTTTGGAGCTTATGAAAATGAACTTGCAAATTTTGA
TAAAAATTTTATCGGTTCTAAAAGTGAAGTGCAAGTAAAATTTCACCATCAGCTCAAAAGCCTTTATATACAAAGTGTTA
TTCATGAAGATGAAAAAACTAAAATTGAAATTTTAAAAAGATTGATCATCAGTTCAAATACTTTAAATTTAGATGACAAG
TCATATGCTAATGAGTTAAAAGAAAGTGGTATAAGTGAAGCAAGTATTAATGCTTTAAGAAAGGCTGTTATTAAAGATAT
TAAGGTTCAAAATATTTCTCAAGACACTGAAAAAACAACTTTTGAACCTATAAAAACAGTTCAAAAAAATGAAAAAAAAT
CATCTAACGAACAAATAAAAAATACAGAAAAAACAAATTCTAAAACTTCGCAAGCACCAAAGAGCAGTATCGTTTCTAAA
GCAAAAAGCGATGATAAAATTTATGTTTTAAAGTCAAGTAAAACAAACCAAGGTGTAGAGTTTGAACTAAATGCAGATTT
GGATAAGGATCAATTAAAAAGTTTTGCTTTAGACGAAAAAGGAAACTATCGTTTTATTAGTGATTTTGATGGGGTTTTAG
AAGGGGGTAAAAAAGAGTTTAAATTTGATGACTATCGTTTAGTGATTTCACAATTTAATCCCAAAACCATACGCATAGTG
GTTTATGCTAAAGAAAAAATTCCTATTGATATATCTTTTAAAGAAGAAAAAATAATTTTTTCAAAAGAAACTACTAGTCA
AAAAAAGACTGAGCAAATTAGTAAAATTTCAGAGAAAAAGCAAGATCAAAAAACAGAAATTAAAACCAAAGTTTTAGAAA
CAAAAAAACAAGATAAAAAAACCAATAATGAAAAAGAAAATCAAAAACCTTTATATGTTTTAGATGTGGATAAAATTTCT
AATGCTGTGGTGTTAAATTTAAGCGATGATTTAGATGAAAAAGAAATAGCAGTATTTGATACCAAAGATCAAAAATTCCG
ATATGTTGTAAGTTTTAAGGGTGTGTTAGAAGGTAATAGAAAAAGCTTTACTTTTGGACAAAATGTGATTACAGTTACTC
AATACAATCCAAAAACCGTTCGTGTGGTTTTGAGTGCTCCTAAAGAATTTAAACTTTTAAAGGAGTTGGATAATAATAAT
CTCACTTTAGGTTTTTATATACAAACTACAAATCAAAATGCAAACAAAAAAGCAACTCAAAGTTCTAGTAAAACTTTAAA
TACAAATTACAAATCAGGAAAGCTTGTGGTTATCGATGCGGGGCATGGTGGAAAAGATAGCGGTGCTTTAAGTGATAAAA
AAGGAAGTTTAAAAGAAAAAGATATAGTTTTAAGTACGGCTTTAAAACTTGGAAATGAACTTAAAAAACGCGGTTATAAA
GTGCTTTATACTAGAAGTTCGGATAAATTTATCAATCTTCGTGATAGAACCAAATATGCCAATGATAAAAGAGCGGATTT
GTTTATTTCTATACATGCTAACGCAGCACCAAATGCCACAAAAGCCAAAAGTAGCGAAGGCGTGGAAACTTTCTTTTTAA
GTCCTGCAAGAAGCGAAAGAAGTAAAAAGGCAGCAGAAAAAGAAAATCAAGGGGATTTTGAAGAGATTAATTATTTCTCA
AAACAAAGTATTTTAAATTTCTTAAATCGTGAAAAAATCGTTGCTTCAAATAAACTAGCCATTGATGTACAAAAAAATAT
ACTCACTCAAACACGCAAAAAGTATAAAATTGTAGATGGCGGTGTAAGAGAAGCACCTTTTTGGGTTTTAGTAGGTGCGC
AAATGCCTGCAATTTTGATTGAAATTGGCTATATTACACATCCAAATGAAGGCAAAAGAATCGCCAATAAAGCTTTTCAA
GATTTATTGGTTAAGGGTATTGCCGATGGAGTTGAGAGTTATTTTTATAACAACCGATGA

Upstream 100 bases:

>100_bases
AAAAAGAAACAGGACTTTTCTTTACAGGTGCAAATGGATATCGTTTAGATAAGCTTATCAGTGTTAAAGAACTTATGGAA
AAATTGGTTAATGGTGAATA

Downstream 100 bases:

>100_bases
AAAAAGCTAAGCTAATTTTTAAGGATAATACTCCTTTTTCTTTGGATTTTGATGATTTTTATTTTAATTCCAAAGATGGC
TTAAATGAAAGCAAATTTGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MAKIFVFLVFAFTTLFGAYENELANFDKNFIGSKSEVQVKFHHQLKSLYIQSVIHEDEKTKIEILKRLIISSNTLNLDDK
SYANELKESGISEASINALRKAVIKDIKVQNISQDTEKTTFEPIKTVQKNEKKSSNEQIKNTEKTNSKTSQAPKSSIVSK
AKSDDKIYVLKSSKTNQGVEFELNADLDKDQLKSFALDEKGNYRFISDFDGVLEGGKKEFKFDDYRLVISQFNPKTIRIV
VYAKEKIPIDISFKEEKIIFSKETTSQKKTEQISKISEKKQDQKTEIKTKVLETKKQDKKTNNEKENQKPLYVLDVDKIS
NAVVLNLSDDLDEKEIAVFDTKDQKFRYVVSFKGVLEGNRKSFTFGQNVITVTQYNPKTVRVVLSAPKEFKLLKELDNNN
LTLGFYIQTTNQNANKKATQSSSKTLNTNYKSGKLVVIDAGHGGKDSGALSDKKGSLKEKDIVLSTALKLGNELKKRGYK
VLYTRSSDKFINLRDRTKYANDKRADLFISIHANAAPNATKAKSSEGVETFFLSPARSERSKKAAEKENQGDFEEINYFS
KQSILNFLNREKIVASNKLAIDVQKNILTQTRKKYKIVDGGVREAPFWVLVGAQMPAILIEIGYITHPNEGKRIANKAFQ
DLLVKGIADGVESYFYNNR

Sequences:

>Translated_659_residues
MAKIFVFLVFAFTTLFGAYENELANFDKNFIGSKSEVQVKFHHQLKSLYIQSVIHEDEKTKIEILKRLIISSNTLNLDDK
SYANELKESGISEASINALRKAVIKDIKVQNISQDTEKTTFEPIKTVQKNEKKSSNEQIKNTEKTNSKTSQAPKSSIVSK
AKSDDKIYVLKSSKTNQGVEFELNADLDKDQLKSFALDEKGNYRFISDFDGVLEGGKKEFKFDDYRLVISQFNPKTIRIV
VYAKEKIPIDISFKEEKIIFSKETTSQKKTEQISKISEKKQDQKTEIKTKVLETKKQDKKTNNEKENQKPLYVLDVDKIS
NAVVLNLSDDLDEKEIAVFDTKDQKFRYVVSFKGVLEGNRKSFTFGQNVITVTQYNPKTVRVVLSAPKEFKLLKELDNNN
LTLGFYIQTTNQNANKKATQSSSKTLNTNYKSGKLVVIDAGHGGKDSGALSDKKGSLKEKDIVLSTALKLGNELKKRGYK
VLYTRSSDKFINLRDRTKYANDKRADLFISIHANAAPNATKAKSSEGVETFFLSPARSERSKKAAEKENQGDFEEINYFS
KQSILNFLNREKIVASNKLAIDVQKNILTQTRKKYKIVDGGVREAPFWVLVGAQMPAILIEIGYITHPNEGKRIANKAFQ
DLLVKGIADGVESYFYNNR
>Mature_658_residues
AKIFVFLVFAFTTLFGAYENELANFDKNFIGSKSEVQVKFHHQLKSLYIQSVIHEDEKTKIEILKRLIISSNTLNLDDKS
YANELKESGISEASINALRKAVIKDIKVQNISQDTEKTTFEPIKTVQKNEKKSSNEQIKNTEKTNSKTSQAPKSSIVSKA
KSDDKIYVLKSSKTNQGVEFELNADLDKDQLKSFALDEKGNYRFISDFDGVLEGGKKEFKFDDYRLVISQFNPKTIRIVV
YAKEKIPIDISFKEEKIIFSKETTSQKKTEQISKISEKKQDQKTEIKTKVLETKKQDKKTNNEKENQKPLYVLDVDKISN
AVVLNLSDDLDEKEIAVFDTKDQKFRYVVSFKGVLEGNRKSFTFGQNVITVTQYNPKTVRVVLSAPKEFKLLKELDNNNL
TLGFYIQTTNQNANKKATQSSSKTLNTNYKSGKLVVIDAGHGGKDSGALSDKKGSLKEKDIVLSTALKLGNELKKRGYKV
LYTRSSDKFINLRDRTKYANDKRADLFISIHANAAPNATKAKSSEGVETFFLSPARSERSKKAAEKENQGDFEEINYFSK
QSILNFLNREKIVASNKLAIDVQKNILTQTRKKYKIVDGGVREAPFWVLVGAQMPAILIEIGYITHPNEGKRIANKAFQD
LLVKGIADGVESYFYNNR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=258, Percent_Identity=32.1705426356589, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87082163, Length=324, Percent_Identity=28.3950617283951, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1790611, Length=234, Percent_Identity=29.0598290598291, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 74715; Mature: 74584

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIFVFLVFAFTTLFGAYENELANFDKNFIGSKSEVQVKFHHQLKSLYIQSVIHEDEKT
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHH
KIEILKRLIISSNTLNLDDKSYANELKESGISEASINALRKAVIKDIKVQNISQDTEKTT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FEPIKTVQKNEKKSSNEQIKNTEKTNSKTSQAPKSSIVSKAKSDDKIYVLKSSKTNQGVE
HHHHHHHHHHCCCCCHHHHHCCHHCCCHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCEE
FELNADLDKDQLKSFALDEKGNYRFISDFDGVLEGGKKEFKFDDYRLVISQFNPKTIRIV
EEECCCCCHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEE
VYAKEKIPIDISFKEEKIIFSKETTSQKKTEQISKISEKKQDQKTEIKTKVLETKKQDKK
EEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNNEKENQKPLYVLDVDKISNAVVLNLSDDLDEKEIAVFDTKDQKFRYVVSFKGVLEGNR
CCCCCCCCCCEEEEEHHHCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCC
KSFTFGQNVITVTQYNPKTVRVVLSAPKEFKLLKELDNNNLTLGFYIQTTNQNANKKATQ
CEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHC
SSSKTLNTNYKSGKLVVIDAGHGGKDSGALSDKKGSLKEKDIVLSTALKLGNELKKRGYK
CCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VLYTRSSDKFINLRDRTKYANDKRADLFISIHANAAPNATKAKSSEGVETFFLSPARSER
EEEEECCCCEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHH
SKKAAEKENQGDFEEINYFSKQSILNFLNREKIVASNKLAIDVQKNILTQTRKKYKIVDG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHEEEECC
GVREAPFWVLVGAQMPAILIEIGYITHPNEGKRIANKAFQDLLVKGIADGVESYFYNNR
CCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKIFVFLVFAFTTLFGAYENELANFDKNFIGSKSEVQVKFHHQLKSLYIQSVIHEDEKT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHH
KIEILKRLIISSNTLNLDDKSYANELKESGISEASINALRKAVIKDIKVQNISQDTEKTT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FEPIKTVQKNEKKSSNEQIKNTEKTNSKTSQAPKSSIVSKAKSDDKIYVLKSSKTNQGVE
HHHHHHHHHHCCCCCHHHHHCCHHCCCHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCEE
FELNADLDKDQLKSFALDEKGNYRFISDFDGVLEGGKKEFKFDDYRLVISQFNPKTIRIV
EEECCCCCHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCCCCCCEEEEEECCCCCEEEEE
VYAKEKIPIDISFKEEKIIFSKETTSQKKTEQISKISEKKQDQKTEIKTKVLETKKQDKK
EEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNNEKENQKPLYVLDVDKISNAVVLNLSDDLDEKEIAVFDTKDQKFRYVVSFKGVLEGNR
CCCCCCCCCCEEEEEHHHCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEEEECCCC
KSFTFGQNVITVTQYNPKTVRVVLSAPKEFKLLKELDNNNLTLGFYIQTTNQNANKKATQ
CEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHC
SSSKTLNTNYKSGKLVVIDAGHGGKDSGALSDKKGSLKEKDIVLSTALKLGNELKKRGYK
CCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
VLYTRSSDKFINLRDRTKYANDKRADLFISIHANAAPNATKAKSSEGVETFFLSPARSER
EEEEECCCCEEEEHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHH
SKKAAEKENQGDFEEINYFSKQSILNFLNREKIVASNKLAIDVQKNILTQTRKKYKIVDG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHEEEECC
GVREAPFWVLVGAQMPAILIEIGYITHPNEGKRIANKAFQDLLVKGIADGVESYFYNNR
CCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]