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The map label for this gene is mnmC [H]

Identifier: 57238140

GI number: 57238140

Start: 1319058

End: 1320899

Strand: Reverse

Name: mnmC [H]

Synonym: CJE1404

Alternate gene names: 57238140

Gene position: 1320899-1319058 (Counterclockwise)

Preceding gene: 57238141

Following gene: 57238139

Centisome position: 74.3

GC content: 27.8

Gene sequence:

>1842_bases
ATGAAAAAAGCTAAGCTAATTTTTAAGGATAATACTCCTTTTTCTTTGGATTTTGATGATTTTTATTTTAATTCCAAAGA
TGGCTTAAATGAAAGCAAATTTGTTTACACTCATTCTTTTGAATGGAAAAATCAAGAAAATTTTATTATCGCAGAGAGTG
GTTTTGGTATAGGTTTAAATTTTTTTCTTACTTTGAAACGCCTTTTAGAAACTACCCCTTCTAAGCGTCCTAAAAAACTT
TTTTATATTAGCGTAGAAGCTTTTTATATCGAAAAAGAACAATTAAGAGAAATTTATCAAAAACTAGAATTTTATGAAGA
GTTTAAAGAGCTTTTAGAACAATTTTTAAAATTTTATCCTAAGGCTAAAGAAGGAATTTATCGTTTTTATTTTGAAGATT
GTTTTTTAGATCTTGTTTTTGAAGATATAGCTGTTTTAAAAGAATTGGATTTTAAAGCTGATGTTTGGTATTTAGATGGA
TTTTCTCCAAATAAAAACTTGCAAATGTTTGATGAAAATTTAATCTTTGAAGTGGCAAGACTTTCTAAAAAAAATACACA
AATTTGCACCTTTTCTTCGGCAAGTTTTTTGCAAAAAAATTTAAAAAAATATGGTTTTAGAGTAGAAAAAACCAAAGGTT
TTAGAAAAAGAGAAATGATTAAAGCTTATTTGGAAAATGAGCTTGAGTTTAAAGACAAAGAAGCGTATTTTTCAAGAACA
TTTTCATCTTTAAAAAATAAAAAAGTAGCTATTATTGGTGCGGGTATATCAAGCGCTGTTTTAGCTTATGAACTTAGTTT
AAGGGGTTTTGAGATAGATGTTTTTGAAAAATATTTAGAATTAGGCAAGGGTGCAAGTGGAAATGAAAGTGGAATTTTAA
GCTCTTTAATCTTAAAACCTAAGGTAAATTTAGGGGAGTTTTCAGAGCTTTCTTTTATAGAAGCAAGCCGTTTTTATAGA
CAAATTTTAGATTTAGAATTTAAGGGTGTGGTGGAGTTTGCACATAATGATTTGATGCAAGAAAGATTTGACACACAAAG
AGAAAATGTTTTGTTTAAAATTTCAAAAAATCAAGCTTTTTTAGAAGAGGGTGGAGTGATTTTTCCAAAAAATTTGGTTA
AAAATCTTTTTGAAAAAAGTAAAGCTTGTATATATTTTAATCATGAATTTCAAGCTTATAAATTTAAAAATGAGTGTTTT
ACTTTAAAATTTAAAAATGATATTGTTAAAAGTGATTATGCGGTTTTGATTTATGCTATGGGTGCAGATACAAAAGATTT
TGTTTTTTATGATGAGATGAAATTAAGCAAAGTAAGAGGTCAAGTTACACATCTAAAACCTTTTTTAGATACCCCATTTC
CTTTATCTTCTAAGGCTTATATTTGCCCTGTAAAAGATGATTTGCAAGTAATTGGAGCAAGTTATGATAGGCTTAATGCA
AGTTTAGAATCAAAAGAAGAAGATGATAAACAAAATATAGAAAATATAGCCGAATTTATAGATAAAAATACAAAATTAGA
AATCATAGGTTCTAAGGTAGGCTTTAGATCTTATTCGAGTGATCGCTTTATGATAGTTGGAAATGCCTATGATGAAGTGT
TTTATAAAGAAGAATATAAAGCTTTATTATGGACAAAAAACAAGGAGCAAAAACCAGCTAAAATGTCTTGTAATTTGTAT
TTTAATTTTGCTCATGGATCAAGGGGTTTTAGCACAAGTGTTTTAGCGGCAAGGTATTTGTGTGCATTGATAAATAATGA
ACCCTTGTGTTTAGAAAAAAAATACATCCACGCTATTCACCCTGCAAGATTTTTGATTCGCAAACTCAAAAAAGGCTTAT
GA

Upstream 100 bases:

>100_bases
CACATCCAAATGAAGGCAAAAGAATCGCCAATAAAGCTTTTCAAGATTTATTGGTTAAGGGTATTGCCGATGGAGTTGAG
AGTTATTTTTATAACAACCG

Downstream 100 bases:

>100_bases
TTAAGCTTTAATTAAGAGTGATACTAATATTTGCTTATGAGAGTAAGTTTTTAACTTTATTTTAACTTTCTTTATTTTAT
TATTTATAGAGAATAAATTT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKKAKLIFKDNTPFSLDFDDFYFNSKDGLNESKFVYTHSFEWKNQENFIIAESGFGIGLNFFLTLKRLLETTPSKRPKKL
FYISVEAFYIEKEQLREIYQKLEFYEEFKELLEQFLKFYPKAKEGIYRFYFEDCFLDLVFEDIAVLKELDFKADVWYLDG
FSPNKNLQMFDENLIFEVARLSKKNTQICTFSSASFLQKNLKKYGFRVEKTKGFRKREMIKAYLENELEFKDKEAYFSRT
FSSLKNKKVAIIGAGISSAVLAYELSLRGFEIDVFEKYLELGKGASGNESGILSSLILKPKVNLGEFSELSFIEASRFYR
QILDLEFKGVVEFAHNDLMQERFDTQRENVLFKISKNQAFLEEGGVIFPKNLVKNLFEKSKACIYFNHEFQAYKFKNECF
TLKFKNDIVKSDYAVLIYAMGADTKDFVFYDEMKLSKVRGQVTHLKPFLDTPFPLSSKAYICPVKDDLQVIGASYDRLNA
SLESKEEDDKQNIENIAEFIDKNTKLEIIGSKVGFRSYSSDRFMIVGNAYDEVFYKEEYKALLWTKNKEQKPAKMSCNLY
FNFAHGSRGFSTSVLAARYLCALINNEPLCLEKKYIHAIHPARFLIRKLKKGL

Sequences:

>Translated_613_residues
MKKAKLIFKDNTPFSLDFDDFYFNSKDGLNESKFVYTHSFEWKNQENFIIAESGFGIGLNFFLTLKRLLETTPSKRPKKL
FYISVEAFYIEKEQLREIYQKLEFYEEFKELLEQFLKFYPKAKEGIYRFYFEDCFLDLVFEDIAVLKELDFKADVWYLDG
FSPNKNLQMFDENLIFEVARLSKKNTQICTFSSASFLQKNLKKYGFRVEKTKGFRKREMIKAYLENELEFKDKEAYFSRT
FSSLKNKKVAIIGAGISSAVLAYELSLRGFEIDVFEKYLELGKGASGNESGILSSLILKPKVNLGEFSELSFIEASRFYR
QILDLEFKGVVEFAHNDLMQERFDTQRENVLFKISKNQAFLEEGGVIFPKNLVKNLFEKSKACIYFNHEFQAYKFKNECF
TLKFKNDIVKSDYAVLIYAMGADTKDFVFYDEMKLSKVRGQVTHLKPFLDTPFPLSSKAYICPVKDDLQVIGASYDRLNA
SLESKEEDDKQNIENIAEFIDKNTKLEIIGSKVGFRSYSSDRFMIVGNAYDEVFYKEEYKALLWTKNKEQKPAKMSCNLY
FNFAHGSRGFSTSVLAARYLCALINNEPLCLEKKYIHAIHPARFLIRKLKKGL
>Mature_613_residues
MKKAKLIFKDNTPFSLDFDDFYFNSKDGLNESKFVYTHSFEWKNQENFIIAESGFGIGLNFFLTLKRLLETTPSKRPKKL
FYISVEAFYIEKEQLREIYQKLEFYEEFKELLEQFLKFYPKAKEGIYRFYFEDCFLDLVFEDIAVLKELDFKADVWYLDG
FSPNKNLQMFDENLIFEVARLSKKNTQICTFSSASFLQKNLKKYGFRVEKTKGFRKREMIKAYLENELEFKDKEAYFSRT
FSSLKNKKVAIIGAGISSAVLAYELSLRGFEIDVFEKYLELGKGASGNESGILSSLILKPKVNLGEFSELSFIEASRFYR
QILDLEFKGVVEFAHNDLMQERFDTQRENVLFKISKNQAFLEEGGVIFPKNLVKNLFEKSKACIYFNHEFQAYKFKNECF
TLKFKNDIVKSDYAVLIYAMGADTKDFVFYDEMKLSKVRGQVTHLKPFLDTPFPLSSKAYICPVKDDLQVIGASYDRLNA
SLESKEEDDKQNIENIAEFIDKNTKLEIIGSKVGFRSYSSDRFMIVGNAYDEVFYKEEYKALLWTKNKEQKPAKMSCNLY
FNFAHGSRGFSTSVLAARYLCALINNEPLCLEKKYIHAIHPARFLIRKLKKGL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=672, Percent_Identity=30.5059523809524, Blast_Score=264, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 71674; Mature: 71674

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAKLIFKDNTPFSLDFDDFYFNSKDGLNESKFVYTHSFEWKNQENFIIAESGFGIGLN
CCCCEEEEECCCCCEECCHHHEECCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHH
FFLTLKRLLETTPSKRPKKLFYISVEAFYIEKEQLREIYQKLEFYEEFKELLEQFLKFYP
HHHHHHHHHHCCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAKEGIYRFYFEDCFLDLVFEDIAVLKELDFKADVWYLDGFSPNKNLQMFDENLIFEVAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHH
LSKKNTQICTFSSASFLQKNLKKYGFRVEKTKGFRKREMIKAYLENELEFKDKEAYFSRT
HCCCCCEEEEECCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSSLKNKKVAIIGAGISSAVLAYELSLRGFEIDVFEKYLELGKGASGNESGILSSLILKP
HHHHCCCEEEEEECCHHHHHEEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KVNLGEFSELSFIEASRFYRQILDLEFKGVVEFAHNDLMQERFDTQRENVLFKISKNQAF
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LEEGGVIFPKNLVKNLFEKSKACIYFNHEFQAYKFKNECFTLKFKNDIVKSDYAVLIYAM
HHCCCEEECHHHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEEEEEE
GADTKDFVFYDEMKLSKVRGQVTHLKPFLDTPFPLSSKAYICPVKDDLQVIGASYDRLNA
CCCCCCEEEECHHHHHHHHCHHHHCCHHCCCCCCCCCCEEEECCCCCHHHHCCCHHHHCC
SLESKEEDDKQNIENIAEFIDKNTKLEIIGSKVGFRSYSSDRFMIVGNAYDEVFYKEEYK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHCCC
ALLWTKNKEQKPAKMSCNLYFNFAHGSRGFSTSVLAARYLCALINNEPLCLEKKYIHAIH
EEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHC
PARFLIRKLKKGL
HHHHHHHHHHHCC
>Mature Secondary Structure
MKKAKLIFKDNTPFSLDFDDFYFNSKDGLNESKFVYTHSFEWKNQENFIIAESGFGIGLN
CCCCEEEEECCCCCEECCHHHEECCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHH
FFLTLKRLLETTPSKRPKKLFYISVEAFYIEKEQLREIYQKLEFYEEFKELLEQFLKFYP
HHHHHHHHHHCCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAKEGIYRFYFEDCFLDLVFEDIAVLKELDFKADVWYLDGFSPNKNLQMFDENLIFEVAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEHHHHHHHHHHH
LSKKNTQICTFSSASFLQKNLKKYGFRVEKTKGFRKREMIKAYLENELEFKDKEAYFSRT
HCCCCCEEEEECCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSSLKNKKVAIIGAGISSAVLAYELSLRGFEIDVFEKYLELGKGASGNESGILSSLILKP
HHHHCCCEEEEEECCHHHHHEEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KVNLGEFSELSFIEASRFYRQILDLEFKGVVEFAHNDLMQERFDTQRENVLFKISKNQAF
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LEEGGVIFPKNLVKNLFEKSKACIYFNHEFQAYKFKNECFTLKFKNDIVKSDYAVLIYAM
HHCCCEEECHHHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEEEEEE
GADTKDFVFYDEMKLSKVRGQVTHLKPFLDTPFPLSSKAYICPVKDDLQVIGASYDRLNA
CCCCCCEEEECHHHHHHHHCHHHHCCHHCCCCCCCCCCEEEECCCCCHHHHCCCHHHHCC
SLESKEEDDKQNIENIAEFIDKNTKLEIIGSKVGFRSYSSDRFMIVGNAYDEVFYKEEYK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHCCC
ALLWTKNKEQKPAKMSCNLYFNFAHGSRGFSTSVLAARYLCALINNEPLCLEKKYIHAIH
EEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHC
PARFLIRKLKKGL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA