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The map label for this gene is htrA [H]

Identifier: 57238100

GI number: 57238100

Start: 1277039

End: 1278457

Strand: Reverse

Name: htrA [H]

Synonym: CJE1363

Alternate gene names: 57238100

Gene position: 1278457-1277039 (Counterclockwise)

Preceding gene: 57238109

Following gene: 57238099

Centisome position: 71.91

GC content: 31.29

Gene sequence:

>1419_bases
ATGAAAAAGATTTTTTTATCATTAAGTTTAGCAAGTGCTTTATTTGCTGCAAGTATTAATTTTAACGAATCGACTGCAAC
GGCTAATCGTGTGAATCCTGCTGCTGGAAATGCAGTGCTTTCTTATCATGATTCTATTAAAGATGCAAAAAAATCAGTGG
TTAATATTTCCACCTCAAAAACTATTACAAGAGCAAATCGTCCAAGCCCTTTGGATGATTTTTTTAATGATCCTTATTTT
AAACAATTTTTTGATTTTGATTTTCCTCAAAGAAAAGGAAAAAATGATAAAGAAGTGGTGAGTTCTTTGGGATCAGGGGT
GATTATTTCAAAAGATGGTTATATAGTAACAAATAATCACGTTGTAGATGATGCTGATACGATTACAGTGAATTTACCAG
GAAGCGACATAGAATATAAAGCAAAACTTATAGGTAAAGATCCAAAAACAGATTTGGCTGTTATAAAAATAGAGGCTAAT
AATCTTTCAGCTATTACTTTTACAAATTCTGATGATTTAATGGAAGGAGATGTTGTTTTTGCACTTGGAAATCCTTTTGG
AGTTGGTTTTAGTGTTACAAGTGGGATAATATCTGCTTTAAATAAAGACAATATAGGTTTAAATCAATATGAAAATTTTA
TACAAACAGATGCTTCTATCAATCCAGGAAATTCAGGTGGAGCTTTGGTGGATAGTCGCGGATATTTAGTAGGTATTAAT
TCAGCTATTCTTTCTCGTGGTGGTGGAAATAACGGCATAGGTTTTGCCATACCTTCAAATATGGTTAAAGATATAGCTAA
AAAACTTATTGAAAAAGGCAAGATTGATAGAGGATTTTTAGGTGTGACTATTTTAGCTTTGCAAGGTGATACTAAAAAAG
CTTACAAAAATCAAGAAGGAGCTTTAATCACTGATGTTCAAAAAGGTTCAAGTGCTGATGAAGCAGGGCTTAAGCGTGGA
GATTTAGTTACTAAAGTTAATGATAAGGTTATAAAAAGTCCTATTGATCTTAAAAATTATATAGGAACTTTAGAGATTGG
TCAAAAAATTTCATTAAGTTACGAAAGAGATGGAGAAAATAAGCAAGCAAGTTTTATTCTTAAAGGTGAAAAAGAAAATC
CTAAAGGCGTACAAAGCGATTTGATTGATGGTTTGAGTTTGAGAAATTTAGATCCAAGACTTAAGGATCGTTTGCAAATT
CCAAAAGATGTTAATGGTGTTTTGGTTGATAGTGTTAAAGAAAAGAGCAAAGGAAAAAATTCTGGTTTCCAAGAGGGTGA
TATTATCATAGGTGTTGGACAAAGTGAAATTAAAAATTTAAAAGATTTAGAACAAGCTTTAAAACAAGTTAATAAAAAAG
AATTTACCAAAGTTTGGGTATATCGCAATGGTTTTGCGACTTTGCTTGTGCTTAAATAA

Upstream 100 bases:

>100_bases
CAAGGTTTTATTTACAATTTTGTAAATTTTCGGTTTTATACTATGAAGACAGTAAAATATTTTACGAGATAATTTATCTT
TAAGAACAAAAGGAAATAAA

Downstream 100 bases:

>100_bases
AAATAACGGAAGGATTTTCCTTTCGTTATAAATTTTAGTTTAAAATTAAAATTAAGTTTAAAATAAAGGAAAACTATGAC
AAATATTCTTATGATAGAAG

Product: protease DO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKKIFLSLSLASALFAASINFNESTATANRVNPAAGNAVLSYHDSIKDAKKSVVNISTSKTITRANRPSPLDDFFNDPYF
KQFFDFDFPQRKGKNDKEVVSSLGSGVIISKDGYIVTNNHVVDDADTITVNLPGSDIEYKAKLIGKDPKTDLAVIKIEAN
NLSAITFTNSDDLMEGDVVFALGNPFGVGFSVTSGIISALNKDNIGLNQYENFIQTDASINPGNSGGALVDSRGYLVGIN
SAILSRGGGNNGIGFAIPSNMVKDIAKKLIEKGKIDRGFLGVTILALQGDTKKAYKNQEGALITDVQKGSSADEAGLKRG
DLVTKVNDKVIKSPIDLKNYIGTLEIGQKISLSYERDGENKQASFILKGEKENPKGVQSDLIDGLSLRNLDPRLKDRLQI
PKDVNGVLVDSVKEKSKGKNSGFQEGDIIIGVGQSEIKNLKDLEQALKQVNKKEFTKVWVYRNGFATLLVLK

Sequences:

>Translated_472_residues
MKKIFLSLSLASALFAASINFNESTATANRVNPAAGNAVLSYHDSIKDAKKSVVNISTSKTITRANRPSPLDDFFNDPYF
KQFFDFDFPQRKGKNDKEVVSSLGSGVIISKDGYIVTNNHVVDDADTITVNLPGSDIEYKAKLIGKDPKTDLAVIKIEAN
NLSAITFTNSDDLMEGDVVFALGNPFGVGFSVTSGIISALNKDNIGLNQYENFIQTDASINPGNSGGALVDSRGYLVGIN
SAILSRGGGNNGIGFAIPSNMVKDIAKKLIEKGKIDRGFLGVTILALQGDTKKAYKNQEGALITDVQKGSSADEAGLKRG
DLVTKVNDKVIKSPIDLKNYIGTLEIGQKISLSYERDGENKQASFILKGEKENPKGVQSDLIDGLSLRNLDPRLKDRLQI
PKDVNGVLVDSVKEKSKGKNSGFQEGDIIIGVGQSEIKNLKDLEQALKQVNKKEFTKVWVYRNGFATLLVLK
>Mature_472_residues
MKKIFLSLSLASALFAASINFNESTATANRVNPAAGNAVLSYHDSIKDAKKSVVNISTSKTITRANRPSPLDDFFNDPYF
KQFFDFDFPQRKGKNDKEVVSSLGSGVIISKDGYIVTNNHVVDDADTITVNLPGSDIEYKAKLIGKDPKTDLAVIKIEAN
NLSAITFTNSDDLMEGDVVFALGNPFGVGFSVTSGIISALNKDNIGLNQYENFIQTDASINPGNSGGALVDSRGYLVGIN
SAILSRGGGNNGIGFAIPSNMVKDIAKKLIEKGKIDRGFLGVTILALQGDTKKAYKNQEGALITDVQKGSSADEAGLKRG
DLVTKVNDKVIKSPIDLKNYIGTLEIGQKISLSYERDGENKQASFILKGEKENPKGVQSDLIDGLSLRNLDPRLKDRLQI
PKDVNGVLVDSVKEKSKGKNSGFQEGDIIIGVGQSEIKNLKDLEQALKQVNKKEFTKVWVYRNGFATLLVLK

Specific function: Serine protease that is required at high temperature. Involved in the degradation of damaged proteins. Shared specificity with hhoA/degQ [H]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Homo sapiens, GI4506141, Length=283, Percent_Identity=34.2756183745583, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI24308541, Length=251, Percent_Identity=35.8565737051793, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI7019477, Length=265, Percent_Identity=32.8301886792453, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI22129776, Length=267, Percent_Identity=32.9588014981273, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786356, Length=408, Percent_Identity=41.1764705882353, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789629, Length=399, Percent_Identity=38.0952380952381, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789630, Length=269, Percent_Identity=40.1486988847584, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646839, Length=263, Percent_Identity=35.361216730038, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 51017; Mature: 51017

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFLSLSLASALFAASINFNESTATANRVNPAAGNAVLSYHDSIKDAKKSVVNISTSK
CCEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHEEEECCCC
TITRANRPSPLDDFFNDPYFKQFFDFDFPQRKGKNDKEVVSSLGSGVIISKDGYIVTNNH
EEECCCCCCCCHHHHCCHHHHHHHCCCCCHHCCCCHHHHHHHHCCCEEEECCCEEEECCE
VVDDADTITVNLPGSDIEYKAKLIGKDPKTDLAVIKIEANNLSAITFTNSDDLMEGDVVF
EECCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEE
ALGNPFGVGFSVTSGIISALNKDNIGLNQYENFIQTDASINPGNSGGALVDSRGYLVGIN
ECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEC
SAILSRGGGNNGIGFAIPSNMVKDIAKKLIEKGKIDRGFLGVTILALQGDTKKAYKNQEG
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHCCCCC
ALITDVQKGSSADEAGLKRGDLVTKVNDKVIKSPIDLKNYIGTLEIGQKISLSYERDGEN
CEEEEECCCCCCCHHCCCCCCEEEECCHHHHCCCCCHHHHCCEEECCCEEEEEECCCCCC
KQASFILKGEKENPKGVQSDLIDGLSLRNLDPRLKDRLQIPKDVNGVLVDSVKEKSKGKN
CEEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHCCCCC
SGFQEGDIIIGVGQSEIKNLKDLEQALKQVNKKEFTKVWVYRNGFATLLVLK
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHCEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKKIFLSLSLASALFAASINFNESTATANRVNPAAGNAVLSYHDSIKDAKKSVVNISTSK
CCEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHEEEECCCC
TITRANRPSPLDDFFNDPYFKQFFDFDFPQRKGKNDKEVVSSLGSGVIISKDGYIVTNNH
EEECCCCCCCCHHHHCCHHHHHHHCCCCCHHCCCCHHHHHHHHCCCEEEECCCEEEECCE
VVDDADTITVNLPGSDIEYKAKLIGKDPKTDLAVIKIEANNLSAITFTNSDDLMEGDVVF
EECCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEE
ALGNPFGVGFSVTSGIISALNKDNIGLNQYENFIQTDASINPGNSGGALVDSRGYLVGIN
ECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEC
SAILSRGGGNNGIGFAIPSNMVKDIAKKLIEKGKIDRGFLGVTILALQGDTKKAYKNQEG
HHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHCCCCC
ALITDVQKGSSADEAGLKRGDLVTKVNDKVIKSPIDLKNYIGTLEIGQKISLSYERDGEN
CEEEEECCCCCCCHHCCCCCCEEEECCHHHHCCCCCHHHHCCEEECCCEEEEEECCCCCC
KQASFILKGEKENPKGVQSDLIDGLSLRNLDPRLKDRLQIPKDVNGVLVDSVKEKSKGKN
CEEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHCCCCC
SGFQEGDIIIGVGQSEIKNLKDLEQALKQVNKKEFTKVWVYRNGFATLLVLK
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHCEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Serine endopeptidases [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1645840; 11677609 [H]