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The map label for this gene is arsB [H]

Identifier: 57238059

GI number: 57238059

Start: 1234739

End: 1236025

Strand: Reverse

Name: arsB [H]

Synonym: CJE1321

Alternate gene names: 57238059

Gene position: 1236025-1234739 (Counterclockwise)

Preceding gene: 57238060

Following gene: 57238058

Centisome position: 69.52

GC content: 26.96

Gene sequence:

>1287_bases
ATGCTTGCTTTTTTTATTTTTTTATCTACTTTAGTTTTACTTTTTTGGCGTCCATGGAATTTACCTATTTGGGTATTTTC
TAGCTTGGGGGCTTTTTTTGTTTTTATTTTTCAATTGGTAGATTTTAAAGATGCTTGTTTTGTTTTTTCTTTGGTTTGGG
ATAGTTCTTTAACTTTGGTTGGGTTGATTATCCTTTCTTTTAGCTTGGAAGCTTTGGGATTTTTTGATTTTATTGCGAGT
AAAATTTTGTATTTTTCTAGAGAGAAAAATCAAGAAAAAATTTATATCAGTACAAAAAAAATGATGTTATTTTTGTTGAT
TTTTGTATTTTTTCTTTCTGCATTTTTTGCTAACGATGGAGCGATTTTAATCATTACACCTATTATTATAGCTTTATTTT
CAACTCTTAAAGATTGTAAAAATCATGCTTTTATTTTAAGTTCTTTTTTACTTAGCCTTTCTTTTTTATGTGATGCAAGT
TCAAACGCTTTGGTTATTTCAAATTTAACCAATATCATCACAGCAAATTATTTTAAGATAGAATTTTTAGAATTTGCTAA
AAATATGTTTTTGCCAAATTTTTTTGTTTTGCTTTCTACTATAGTTACGGTTTTTGTGCTTTATGTGAGAGTTTTACCTA
AAAGGCTTGAATTTAAATTAATCAAAAAAGAGCAAATTTCATTGAAATTGTTTTTTCTTTGCATAGTATTTTTATTTCTT
TTTGTTATCAGTTTTTTTATAGGTGAAATTTTTAATATTAAAATAAGTTTTTTTGCTTTGCTTTGGGCGGGAATTTTTTG
GCTTATTATTTTAAAAATTCAAGGTAAAAAAAGTATTAAAATTCTTTTTGAAGCACCTTATGGAGTTTTGCTTTTTAGTT
TTGGGCTTTATATGGTGGTTTTTGCTTTACATAAAATAGGTGTGAGTGAAATTTTGGTTAAAAGCTATGCTTTTTTGATG
CAAGATAAGAGTGGAATTTTTGGTGTGGCTTTGATTTCAGCTTTTGGATCTTCTGTGTTTAATAATTTGCCTATGGTTTT
AATCGGAGATTTGGCATTAAAAGAATATTTTGAGAATTTTTCTTTTGATTCTTTGATGATTTATGCACATCTTTTAGGTG
TAAATATAGGTCCAAAACTTACGCCAATAGGTTCTTTAGCAACTTTGCTTTGGCTTGGAGTTTTGGCTAGAAAAGGCATT
AATATTTCTTTTTGGCAATATTGTAAATTTGGCTTTTTGATAACTTTACCTGTGCTTGTTTTTTCTCTTTTTGCTCTTAT
TGTCTAA

Upstream 100 bases:

>100_bases
ACCGCCAACCATTTTTGCTGCAAGTCAAATCAGTGGAATCACTCCTGCAGCGTTAGATATTTTACAAATTTATATCAAAA
TGCAAAAGAAAAAAGTTTAA

Downstream 100 bases:

>100_bases
ATATCAAAACTTGTTAAACAAAGAAAAAATTTATTTTAATTTCGATAAACTTTTAACTTGGTTAAAAAATAAAATCGAAA
GGAGCAAGTTATGGCTACAT

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLAFFIFLSTLVLLFWRPWNLPIWVFSSLGAFFVFIFQLVDFKDACFVFSLVWDSSLTLVGLIILSFSLEALGFFDFIAS
KILYFSREKNQEKIYISTKKMMLFLLIFVFFLSAFFANDGAILIITPIIIALFSTLKDCKNHAFILSSFLLSLSFLCDAS
SNALVISNLTNIITANYFKIEFLEFAKNMFLPNFFVLLSTIVTVFVLYVRVLPKRLEFKLIKKEQISLKLFFLCIVFLFL
FVISFFIGEIFNIKISFFALLWAGIFWLIILKIQGKKSIKILFEAPYGVLLFSFGLYMVVFALHKIGVSEILVKSYAFLM
QDKSGIFGVALISAFGSSVFNNLPMVLIGDLALKEYFENFSFDSLMIYAHLLGVNIGPKLTPIGSLATLLWLGVLARKGI
NISFWQYCKFGFLITLPVLVFSLFALIV

Sequences:

>Translated_428_residues
MLAFFIFLSTLVLLFWRPWNLPIWVFSSLGAFFVFIFQLVDFKDACFVFSLVWDSSLTLVGLIILSFSLEALGFFDFIAS
KILYFSREKNQEKIYISTKKMMLFLLIFVFFLSAFFANDGAILIITPIIIALFSTLKDCKNHAFILSSFLLSLSFLCDAS
SNALVISNLTNIITANYFKIEFLEFAKNMFLPNFFVLLSTIVTVFVLYVRVLPKRLEFKLIKKEQISLKLFFLCIVFLFL
FVISFFIGEIFNIKISFFALLWAGIFWLIILKIQGKKSIKILFEAPYGVLLFSFGLYMVVFALHKIGVSEILVKSYAFLM
QDKSGIFGVALISAFGSSVFNNLPMVLIGDLALKEYFENFSFDSLMIYAHLLGVNIGPKLTPIGSLATLLWLGVLARKGI
NISFWQYCKFGFLITLPVLVFSLFALIV
>Mature_428_residues
MLAFFIFLSTLVLLFWRPWNLPIWVFSSLGAFFVFIFQLVDFKDACFVFSLVWDSSLTLVGLIILSFSLEALGFFDFIAS
KILYFSREKNQEKIYISTKKMMLFLLIFVFFLSAFFANDGAILIITPIIIALFSTLKDCKNHAFILSSFLLSLSFLCDAS
SNALVISNLTNIITANYFKIEFLEFAKNMFLPNFFVLLSTIVTVFVLYVRVLPKRLEFKLIKKEQISLKLFFLCIVFLFL
FVISFFIGEIFNIKISFFALLWAGIFWLIILKIQGKKSIKILFEAPYGVLLFSFGLYMVVFALHKIGVSEILVKSYAFLM
QDKSGIFGVALISAFGSSVFNNLPMVLIGDLALKEYFENFSFDSLMIYAHLLGVNIGPKLTPIGSLATLLWLGVLARKGI
NISFWQYCKFGFLITLPVLVFSLFALIV

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=431, Percent_Identity=32.9466357308585, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 48840; Mature: 48840

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHC
>Mature Secondary Structure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HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA