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The map label for this gene is gidA [H]

Identifier: 57238060

GI number: 57238060

Start: 1236026

End: 1237885

Strand: Reverse

Name: gidA [H]

Synonym: CJE1322

Alternate gene names: 57238060

Gene position: 1237885-1236026 (Counterclockwise)

Preceding gene: 57238061

Following gene: 57238059

Centisome position: 69.63

GC content: 33.39

Gene sequence:

>1860_bases
ATGTTTGATGTCATTGTTATAGGTGGTGGACATGCAGGTGTTGAAGCAAGTGCTGCTGCTGCTAGAATGGGTAAAAAAAC
CTTACTTTTAACTACATTGATTGAGCAAATAGGAGCGGCAAGTTGTAATCCAGCCATAGGTGGACTTGCTAAAGGGCATT
TGGTAAAAGAACTTGATGCCATGGGTGGTCTTATGGGTGAAATCACCGATGAAGCAGGGATACAATTTCGCATTTTAAAT
GAAAGTAAGGGTGTAGCCGTTCAAGGAAGTAGGGCACAAATTGATATGGATAAATACCGTATCATTGCACGTAATAAACT
TTTAAAACTTCCAAATTTAGAAATTTCTCAAGAACAAGCGAGTGTTTTAATCGTAGAAAATGATGAAGTTAAAGGCGTAA
AAACAAATTTAGAAAATATATATTTTGCCAAAAAAGTCATCCTAACAACAGGAACTTTTTTAAATGGACTTATTCACGTA
GGTGAAAATAAACTTCAAGCAGGAAGGGTAGGGGAGCTTGCTTCGGTAAATTTAGGAAATTATCTGCAAACTTTGGGTTT
AAAAATGGGACGTTTAAAAACAGGAACTTGCCCAAGAGTAGATGCAAAAAGCATTGATTTTAGTGTGCTTGAAATTCAAG
ATGGTGATGTAAATCCTAAAGCTTTTAGTTTTCGTTCAAGAAATTTTAATCCTACTCAACTTCCTTGTTATATTGCACGC
ACTAATACAACTACACATGAGATTATTAAGAATAATTTTTATCGTGCTCCACTTTTTACGGGACAGATTGAAGGTGTGGG
GCCAAGGTATTGTCCTTCTATAGAAGATAAGATCAATCGTTTTAGCGACAAAGAAAGTCATCATCTTTTTATAGAGCCTC
AAACTATCGATGCGACGGAGTATTATATTAACGGTTTTTCTACTTCTTTACCTTATGAAGTACAAACTCAAATGTTGCGC
TCTGTAAAAGGTTTTGAAAATGCTAAAATAACCCGTTTTGGTTATGCTATAGAATATGACTATATAGAGCCAACTGAACT
TAAGCATACTTTAGAACTTAAAAAGATTAAAAATCTTTATTGTGCAGGACAGATTAATGGAACTACAGGTTATGAAGAGG
CTGCAGCACAAGGCTTTATGGCGGGAATCAATGCAAGCTTAAGCATAGATATGAAAGAGCCTTTGATTTTAAGGCGTGAT
GAAGCTTATATAGGAGTTTTAATCGATGATTTAGTAGTAAAAGGTACAAAAGAACCTTATAGAATGTTTACTTCAAGAGC
AGAATTTAGACTGCTTTTAAGGGAAGAAAATGCTATACTTAGACTTGGAAAATATGGCTATGATTTGGGGCTTTTAAGCG
AGCAAGATTTTACTTATATACAAAATATCGCAAACAATCTTCAAAAAGGACTTGAATTTTTACTTTCTAAAGAATTCACG
CCCAATAACCAAAACAATGCTTTTTTAGAGAGTTTGGGAGAAGATAAAATTTCTTCTATAGTCAATCTTCAAAAAATTGT
CGCAAGAGCAAGTTTTGATATAGAAAAATTAAAAAAACTTGACCCTATATTTGAAACTATGGATCATTACTCCTTAAGAG
AGATTTTAAATGAGGCAAAGTATTATCATTACATTAGTATGCAAAAAGCTCAAGTAGAAAAAATGAAAAATCTTAGTGAG
CTTAAAATTCCTGAAAATTTTGATTTTAAAAGTGTAAGTGGTTTGAGTAATGAGGTTGTAGAAAAACTTAATCATCATAA
ACCGCCAACCATTTTTGCTGCAAGTCAAATCAGTGGAATCACTCCTGCAGCGTTAGATATTTTACAAATTTATATCAAAA
TGCAAAAGAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
AGAAGCTAAATAAATTTTTGTTAGACTTAAAACGAGTTTGTTTTAAGTCTAAGTATTATATAATCTTTCTTTTAAAAATT
CACATTTTTCAAGGCAAAAT

Downstream 100 bases:

>100_bases
ATGCTTGCTTTTTTTATTTTTTTATCTACTTTAGTTTTACTTTTTTGGCGTCCATGGAATTTACCTATTTGGGTATTTTC
TAGCTTGGGGGCTTTTTTTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGLMGEITDEAGIQFRILN
ESKGVAVQGSRAQIDMDKYRIIARNKLLKLPNLEISQEQASVLIVENDEVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDAKSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLR
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAAQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYDLGLLSEQDFTYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNLQKIVARASFDIEKLKKLDPIFETMDHYSLREILNEAKYYHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKV

Sequences:

>Translated_619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGLMGEITDEAGIQFRILN
ESKGVAVQGSRAQIDMDKYRIIARNKLLKLPNLEISQEQASVLIVENDEVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDAKSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLR
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAAQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYDLGLLSEQDFTYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNLQKIVARASFDIEKLKKLDPIFETMDHYSLREILNEAKYYHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKV
>Mature_619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGLMGEITDEAGIQFRILN
ESKGVAVQGSRAQIDMDKYRIIARNKLLKLPNLEISQEQASVLIVENDEVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDAKSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLR
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAAQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYDLGLLSEQDFTYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNLQKIVARASFDIEKLKKLDPIFETMDHYSLREILNEAKYYHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKV

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=41.2225705329154, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=39.6681749622926, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=38.7905604719764, Blast_Score=435, Evalue=1e-122,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=51.2155591572123, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=40.9967845659164, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321202, Length=633, Percent_Identity=41.5481832543444, Blast_Score=453, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=41.8495297805643, Blast_Score=440, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69201; Mature: 69201

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDA
CEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MGGLMGEITDEAGIQFRILNESKGVAVQGSRAQIDMDKYRIIARNKLLKLPNLEISQEQA
HCCHHHHCCCCCCCEEEEEECCCCEEEECCCCEECHHHHEEEEHHCEEECCCCCCCCCCC
SVLIVENDEVKGVKTNLENIYFAKKVILTTGTFLNGLIHVGENKLQAGRVGELASVNLGN
CEEEEECCCCCCHHHHHHHHHHHHHHEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHH
YLQTLGLKMGRLKTGTCPRVDAKSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
HHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEE
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATE
CCCCHHHHHHCCCEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCHH
YYINGFSTSLPYEVQTQMLRSVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLY
HEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCEE
CAGQINGTTGYEEAAAQGFMAGINASLSIDMKEPLILRRDEAYIGVLIDDLVVKGTKEPY
EEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEHHHHHCCCCCHH
RMFTSRAEFRLLLREENAILRLGKYGYDLGLLSEQDFTYIQNIANNLQKGLEFLLSKEFT
HHHHCCHHEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PNNQNNAFLESLGEDKISSIVNLQKIVARASFDIEKLKKLDPIFETMDHYSLREILNEAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHH
YYHYISMQKAQVEKMKNLSELKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGI
EEEEEEHHHHHHHHHCCHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCC
TPAALDILQIYIKMQKKKV
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDA
CEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MGGLMGEITDEAGIQFRILNESKGVAVQGSRAQIDMDKYRIIARNKLLKLPNLEISQEQA
HCCHHHHCCCCCCCEEEEEECCCCEEEECCCCEECHHHHEEEEHHCEEECCCCCCCCCCC
SVLIVENDEVKGVKTNLENIYFAKKVILTTGTFLNGLIHVGENKLQAGRVGELASVNLGN
CEEEEECCCCCCHHHHHHHHHHHHHHEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHH
YLQTLGLKMGRLKTGTCPRVDAKSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
HHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEE
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATE
CCCCHHHHHHCCCEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCHH
YYINGFSTSLPYEVQTQMLRSVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLY
HEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCEE
CAGQINGTTGYEEAAAQGFMAGINASLSIDMKEPLILRRDEAYIGVLIDDLVVKGTKEPY
EEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEHHHHHCCCCCHH
RMFTSRAEFRLLLREENAILRLGKYGYDLGLLSEQDFTYIQNIANNLQKGLEFLLSKEFT
HHHHCCHHEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PNNQNNAFLESLGEDKISSIVNLQKIVARASFDIEKLKKLDPIFETMDHYSLREILNEAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHH
YYHYISMQKAQVEKMKNLSELKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGI
EEEEEEHHHHHHHHHCCHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCC
TPAALDILQIYIKMQKKKV
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA