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The map label for this gene is gidB [H]

Identifier: 57237822

GI number: 57237822

Start: 1005259

End: 1005825

Strand: Direct

Name: gidB [H]

Synonym: CJE1077

Alternate gene names: 57237822

Gene position: 1005259-1005825 (Clockwise)

Preceding gene: 57237821

Following gene: 57237825

Centisome position: 56.54

GC content: 25.4

Gene sequence:

>567_bases
ATGATTTTTAAAGATTATGATTTTTTGCAAAACTACGATTTAAAAAACTTTGAAGAAAAGGTAAAAATTTATAAAGAATT
ATTATCTAAATTTAACCGCATTCACAATCTTACTCATTTAAAAAATATAGATGAAAATATCTTTGATAGTATTAAAATTT
TAGATTTTTATGATTTTTCTAAAGCAAAAAATATTGCCGATATAGGAAGTGGCGCAGGATTTCCAGCTGTATTTTTAGCT
TTTTTACTGCAAGGCAATTTTCATCTTTTCGAACCCAATCCCAAAAAAGCTGCCTTTTTGCGGACACTAAAAATAGAATG
CGAACTTTCAAATTTACATATTTACAAAGAAAAAGTTCAAGAATACCAAAATATCTTTAAAGCCGATATCATCACTTCAA
GAGCCTTAATGGATGTCAAGCCTTTGCTTGAAATTTGCAAAAATCTAAAAGATGAAAATACGGTTTTTATTTTATGGAAA
GGTAGTGAAATTTATCAAGAACTTGAAAATATAAAAGATTATGAAATTTTTGAAAATAATTTAAGAAAATATTGTATTTT
AAAGTAG

Upstream 100 bases:

>100_bases
AGAAAAAATCATCATACGCATACCGATTTTAATAGAACCTAATCGTTTTAATGTAGAGTATTTAAACATAAAACAAACTC
AAATGGGACATCTTAAATAA

Downstream 100 bases:

>100_bases
CCCTAAGGCTACTTTTTATTCTGCTAGGTCAGCGTTAAAACTGATATCCACTAAAGGCCAAGAAATTCCACCATGTCCTG
CAGCAACATCGCTTAATGCT

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MIFKDYDFLQNYDLKNFEEKVKIYKELLSKFNRIHNLTHLKNIDENIFDSIKILDFYDFSKAKNIADIGSGAGFPAVFLA
FLLQGNFHLFEPNPKKAAFLRTLKIECELSNLHIYKEKVQEYQNIFKADIITSRALMDVKPLLEICKNLKDENTVFILWK
GSEIYQELENIKDYEIFENNLRKYCILK

Sequences:

>Translated_188_residues
MIFKDYDFLQNYDLKNFEEKVKIYKELLSKFNRIHNLTHLKNIDENIFDSIKILDFYDFSKAKNIADIGSGAGFPAVFLA
FLLQGNFHLFEPNPKKAAFLRTLKIECELSNLHIYKEKVQEYQNIFKADIITSRALMDVKPLLEICKNLKDENTVFILWK
GSEIYQELENIKDYEIFENNLRKYCILK
>Mature_188_residues
MIFKDYDFLQNYDLKNFEEKVKIYKELLSKFNRIHNLTHLKNIDENIFDSIKILDFYDFSKAKNIADIGSGAGFPAVFLA
FLLQGNFHLFEPNPKKAAFLRTLKIECELSNLHIYKEKVQEYQNIFKADIITSRALMDVKPLLEICKNLKDENTVFILWK
GSEIYQELENIKDYEIFENNLRKYCILK

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=143, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 22323; Mature: 22323

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKDYDFLQNYDLKNFEEKVKIYKELLSKFNRIHNLTHLKNIDENIFDSIKILDFYDFS
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KAKNIADIGSGAGFPAVFLAFLLQGNFHLFEPNPKKAAFLRTLKIECELSNLHIYKEKVQ
HHCCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHEEEEEECHHHHHHHHHH
EYQNIFKADIITSRALMDVKPLLEICKNLKDENTVFILWKGSEIYQELENIKDYEIFENN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHC
LRKYCILK
CHHHCCCC
>Mature Secondary Structure
MIFKDYDFLQNYDLKNFEEKVKIYKELLSKFNRIHNLTHLKNIDENIFDSIKILDFYDFS
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KAKNIADIGSGAGFPAVFLAFLLQGNFHLFEPNPKKAAFLRTLKIECELSNLHIYKEKVQ
HHCCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHEEEEEECHHHHHHHHHH
EYQNIFKADIITSRALMDVKPLLEICKNLKDENTVFILWKGSEIYQELENIKDYEIFENN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHC
LRKYCILK
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA