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The map label for this gene is dsbA [C]

Identifier: 57237707

GI number: 57237707

Start: 891829

End: 892500

Strand: Direct

Name: dsbA [C]

Synonym: CJE0954

Alternate gene names: 57237707

Gene position: 891829-892500 (Clockwise)

Preceding gene: 57237706

Following gene: 57237708

Centisome position: 50.16

GC content: 26.64

Gene sequence:

>672_bases
TTGAAAATTTTATTTAACCAAGGATATAATATGCGTAAATTTTTTTGCAAATTTGTTTTAACGCTTGTATTTTGTTCAAG
TTTTGCATTAGCAAATAATAGTTTTATTACTCTTAATCCAAGTTTACCTAGCTCTGAAAATAGTGTAATAGAAGCATTTT
CTTATAAATGTATACATTGTTACAATCATCATAAATTTGGAACCCTTGAAAAACTGAGAGAAGCTTTTCCAAATTTACAT
TTTAAACTCTATCCTGTAAGTTTAATGAATGGGGAATTTTCCAAAGAAATGAATGAGCTTTTTGCCTTTGCTCAATATAA
AGATGAACAAAATGGTAAAGACGCGAGTTATTCTGATAGTTTATCTTATAAACTTGCTGATGTTTATTTTGTTTCTTATT
TTTTAAACAAGCAAAGAAATCTTTCAAATTTAGATGAATTTTACGACATAGGTTTAAAGGCTATGAATGTAAATAAAAAT
GAAGTATTAAATTTTTTAAATACCCCAAAAGCTAAAGAAATTTTAAGCGAGTTTCAAAGAGCTAATGATATAGCTAAAAC
CTATGGCACCCCTGCTTTTGTTGTTAATGGAAAATATCAAATTAACCCAAGTGCAATCAATTCGATGCAAGATTTAGAAG
ATCTAGTTAAAAAATTAAGTAATATGAAATAA

Upstream 100 bases:

>100_bases
AAGCAATGCCTTTTAGTGTTGATCAAGCATTCAATCCTAAAAAATAAATAAGCCCAAATTTATTTTTGGGTTTATTTATT
TTTTTTAAATAAAATTATCT

Downstream 100 bases:

>100_bases
TAAATTTTTAAGGCAATAAAAATTGCCTTAAAAATTATTTTTTGATCATATTTGTATAAGGTCCATAGCGATGTTGGGTT
GTAGAAAAGCTATCATCATA

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKILFNQGYNMRKFFCKFVLTLVFCSSFALANNSFITLNPSLPSSENSVIEAFSYKCIHCYNHHKFGTLEKLREAFPNLH
FKLYPVSLMNGEFSKEMNELFAFAQYKDEQNGKDASYSDSLSYKLADVYFVSYFLNKQRNLSNLDEFYDIGLKAMNVNKN
EVLNFLNTPKAKEILSEFQRANDIAKTYGTPAFVVNGKYQINPSAINSMQDLEDLVKKLSNMK

Sequences:

>Translated_223_residues
MKILFNQGYNMRKFFCKFVLTLVFCSSFALANNSFITLNPSLPSSENSVIEAFSYKCIHCYNHHKFGTLEKLREAFPNLH
FKLYPVSLMNGEFSKEMNELFAFAQYKDEQNGKDASYSDSLSYKLADVYFVSYFLNKQRNLSNLDEFYDIGLKAMNVNKN
EVLNFLNTPKAKEILSEFQRANDIAKTYGTPAFVVNGKYQINPSAINSMQDLEDLVKKLSNMK
>Mature_223_residues
MKILFNQGYNMRKFFCKFVLTLVFCSSFALANNSFITLNPSLPSSENSVIEAFSYKCIHCYNHHKFGTLEKLREAFPNLH
FKLYPVSLMNGEFSKEMNELFAFAQYKDEQNGKDASYSDSLSYKLADVYFVSYFLNKQRNLSNLDEFYDIGLKAMNVNKN
EVLNFLNTPKAKEILSEFQRANDIAKTYGTPAFVVNGKYQINPSAINSMQDLEDLVKKLSNMK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 25713; Mature: 25713

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFNQGYNMRKFFCKFVLTLVFCSSFALANNSFITLNPSLPSSENSVIEAFSYKCIHC
CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHEE
YNHHKFGTLEKLREAFPNLHFKLYPVSLMNGEFSKEMNELFAFAQYKDEQNGKDASYSDS
ECCCCCCHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSYKLADVYFVSYFLNKQRNLSNLDEFYDIGLKAMNVNKNEVLNFLNTPKAKEILSEFQR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCHHHHHHHHCCCCHHHHHHHHHH
ANDIAKTYGTPAFVVNGKYQINPSAINSMQDLEDLVKKLSNMK
HHHHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILFNQGYNMRKFFCKFVLTLVFCSSFALANNSFITLNPSLPSSENSVIEAFSYKCIHC
CEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHEE
YNHHKFGTLEKLREAFPNLHFKLYPVSLMNGEFSKEMNELFAFAQYKDEQNGKDASYSDS
ECCCCCCHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSYKLADVYFVSYFLNKQRNLSNLDEFYDIGLKAMNVNKNEVLNFLNTPKAKEILSEFQR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCHHHHHHHHCCCCHHHHHHHHHH
ANDIAKTYGTPAFVVNGKYQINPSAINSMQDLEDLVKKLSNMK
HHHHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]