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The map label for this gene is folD [H]

Identifier: 57237696

GI number: 57237696

Start: 879156

End: 880004

Strand: Direct

Name: folD [H]

Synonym: CJE0942

Alternate gene names: 57237696

Gene position: 879156-880004 (Clockwise)

Preceding gene: 57237688

Following gene: 57237697

Centisome position: 49.45

GC content: 32.98

Gene sequence:

>849_bases
ATGACTTTGCTTGATGGTAAAGCTTTAAGCGCAAAAATCAAAGAAGAATTAAAAGAAAAAAATCAATTTTTGAAAAGCAA
AGGGATTGAAAGCTGTTTAGCTGTTATCTTGGTTGGAGATGATCCTGCAAGTCAAACTTATGTAAAATCTAAGGCTAAAG
CCTGTGAAGAATGTGGAATTAAATCTTTAGTTTATCATCTTAATGAAAATACTACACAAAATGAACTTTTGGCATTGATT
AATACCTTAAATCACGATGATAGTGTTCATGGAATTTTGGTTCAACTTCCCTTACCTGATCATATTTGTAAAGATTTAAT
ACTTGAAAGCATTATAAGTAGTAAAGATGTTGATGGCTTTCATCCTATTAATGTAGGATATTTAAATTTAGGCTTAGAAA
GTGGGTTTTTGCCTTGTACTCCACTTGGAGTTATGAAGCTTTTGAAGGCTTATGAAATTGATTTAGAAGGAAAAGACGCT
GTTATTATAGGTGCTTCAAATATAGTAGGTCGTCCTATGGCTACTATGCTTTTAAACGCTGGAGCTACAGTAAGCGTTTG
TCATATTAAAACCAAAGATTTGAGCCTTTATACTAGGCAAGCTGATTTGATTATAGTAGCAGCAGGTTGTGTGAATTTAC
TTCGATCGGATATGGTAAAAGAAGGAGTGATTGTTATTGATGTTGGAATCAATCGTCTTGAAAGTGGAAAGATAGTAGGA
GATGTAGATTTTGAAGAGGTATCTAAAAAATCTAGTTATATAACTCCTGTTCCAGGTGGAGTAGGTCCTATGACTATAGC
CATGCTTTTAGAAAACACAGTTAAATCTGCTAAAAATAGACTTAATTAG

Upstream 100 bases:

>100_bases
AAACATTAAGACAATTTTCAAAGTTTCACCTTGTTAATGAAGTTTTAATATGATACAATTTTTACTTTAAAAAAGTGATT
AAATTTATAGAAGGAAAATA

Downstream 100 bases:

>100_bases
GGAAAATCTATGGAAATTTTAAAGAAATTATATAAATTTTCACAGTCTTGGACTGGAACTGTAGTTATTGTTCTTTTGGT
GATTTTTTTCTTTATACAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDDPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALI
NTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDA
VIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVIDVGINRLESGKIVG
DVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRLN

Sequences:

>Translated_282_residues
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDDPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALI
NTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDA
VIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVIDVGINRLESGKIVG
DVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRLN
>Mature_281_residues
TLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDDPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALIN
TLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDAV
IIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVIDVGINRLESGKIVGD
VDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRLN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.7586206896552, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=45.5479452054795, Blast_Score=221, Evalue=4e-58,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.6081081081081, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786741, Length=275, Percent_Identity=54.5454545454545, Blast_Score=287, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17568735, Length=261, Percent_Identity=40.9961685823755, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=45.8333333333333, Blast_Score=251, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=41.8918918918919, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=287, Percent_Identity=27.8745644599303, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=45.6081081081081, Blast_Score=247, Evalue=5e-66,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=45.6081081081081, Blast_Score=247, Evalue=6e-66,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=40.8304498269896, Blast_Score=217, Evalue=6e-57,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=40.8304498269896, Blast_Score=217, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=40.8304498269896, Blast_Score=217, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=40.8304498269896, Blast_Score=217, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30353; Mature: 30222

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDDPASQTYVKSKAKACEECGI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCH
KSLVYHLNENTTQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
HPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDAVIIGASNIVGRPMATMLLNA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCC
GATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVIDVGINRLESGKIVG
CCEEEEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
DVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRLN
CCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGDDPASQTYVKSKAKACEECGI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCH
KSLVYHLNENTTQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
HPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDAVIIGASNIVGRPMATMLLNA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCC
GATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVIDVGINRLESGKIVG
CCEEEEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
DVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRLN
CCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA