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The map label for this gene is tcpJ [H]

Identifier: 57237666

GI number: 57237666

Start: 850478

End: 851254

Strand: Direct

Name: tcpJ [H]

Synonym: CJE0912

Alternate gene names: 57237666

Gene position: 850478-851254 (Clockwise)

Preceding gene: 57237665

Following gene: 57237667

Centisome position: 47.84

GC content: 24.58

Gene sequence:

>777_bases
ATGATATTTTTCATAATAATTCTAGGGGCTTGTTTGGGTTCTTTTTGCACAAGTTTGGCAAGTAGGATAATTGAAAAAAA
GCCCTTTTTTATCTCGCGCTCTTTTTGTTTTTCTTGTGATACAAAATTAAAATACTATGAAATTATTCCTATTTTTTCTT
ATATTTTTTTAAAGGCAAAATGTCAAACTTGTAAATGTCATTTGCCTATTTCTTTGTTAATCAATGAAATTTTAGGCGCA
ATTTTGCTTATTTTGGTTTATTCTTTAAGTCAAAATTTTTATGATTTTTTATTTTTATCTCTATTTTTATTTAATCTTTT
CTTACTTTCTTTAATTGATATTAAACTCAAAGCCGTACCACAAATTTTACTTTGGAGTGCTTTTTTATTTGCTTTTTTTT
ACGCCTTTAGGGAGAGTGAAATTTTACATTTTTTGATTTTTAAAGAGTTTAGTGGAGGTTTTTTACTAAATGCTTTTAGT
TTTGGAGGATTTGTTTTTTTATTAAAAAGTTTGGTTTTTTTTCTAATGAATTTCCAAAAAAAAGATGAAATTTTAGAAAA
TTTGGGTGATGCTGATATTATTATTATGTCTTGTATAGGTGGAATTTTAGGTTTTGAGTATGGATTTTTAGTATTATTTA
TAGCTTCTATTTTGACTTTGCCTTTTTTTATTTTTTTTAGAATTAAAGCAATAAAAGAGCAAGAACTTGCTATGATACCC
TTTTTAAATATCGCTTTTGTGGCAGTTTTATTTTATAAAAACTTAGGATTATTCTAA

Upstream 100 bases:

>100_bases
CTGAAATTTATTTTAGTGAAACTTTATTTCCATCTCTTACCAAGAGAGAATTTAAAAGGATTATTAAAGAATTTAGAAAT
AGAGAAAGAACTTTTGGAAA

Downstream 100 bases:

>100_bases
TGAAGCTTAGTTTAAGATATGTTTTAAATCAATTTTTAAGTACGAATTTGTCTATATTTTTCGTACTTTTTGCTATTGTA
TCTATGGTGTTTTTTATTCA

Product: A24 family peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MIFFIIILGACLGSFCTSLASRIIEKKPFFISRSFCFSCDTKLKYYEIIPIFSYIFLKAKCQTCKCHLPISLLINEILGA
ILLILVYSLSQNFYDFLFLSLFLFNLFLLSLIDIKLKAVPQILLWSAFLFAFFYAFRESEILHFLIFKEFSGGFLLNAFS
FGGFVFLLKSLVFFLMNFQKKDEILENLGDADIIIMSCIGGILGFEYGFLVLFIASILTLPFFIFFRIKAIKEQELAMIP
FLNIAFVAVLFYKNLGLF

Sequences:

>Translated_258_residues
MIFFIIILGACLGSFCTSLASRIIEKKPFFISRSFCFSCDTKLKYYEIIPIFSYIFLKAKCQTCKCHLPISLLINEILGA
ILLILVYSLSQNFYDFLFLSLFLFNLFLLSLIDIKLKAVPQILLWSAFLFAFFYAFRESEILHFLIFKEFSGGFLLNAFS
FGGFVFLLKSLVFFLMNFQKKDEILENLGDADIIIMSCIGGILGFEYGFLVLFIASILTLPFFIFFRIKAIKEQELAMIP
FLNIAFVAVLFYKNLGLF
>Mature_258_residues
MIFFIIILGACLGSFCTSLASRIIEKKPFFISRSFCFSCDTKLKYYEIIPIFSYIFLKAKCQTCKCHLPISLLINEILGA
ILLILVYSLSQNFYDFLFLSLFLFNLFLLSLIDIKLKAVPQILLWSAFLFAFFYAFRESEILHFLIFKEFSGGFLLNAFS
FGGFVFLLKSLVFFLMNFQKKDEILENLGDADIIIMSCIGGILGFEYGFLVLFIASILTLPFFIFFRIKAIKEQELAMIP
FLNIAFVAVLFYKNLGLF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tcpA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29738; Mature: 29738

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1680773; 7894059; 11179381 [H]