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The map label for this gene is cca [H]

Identifier: 57237634

GI number: 57237634

Start: 817776

End: 818807

Strand: Direct

Name: cca [H]

Synonym: CJE0880

Alternate gene names: 57237634

Gene position: 817776-818807 (Clockwise)

Preceding gene: 57237633

Following gene: 57237635

Centisome position: 46.0

GC content: 29.55

Gene sequence:

>1032_bases
TTGGTTGGAGGTAGCGTTAGGGATTTGTTTTTAGGCTTAAAAATTTGTGACTATGATATAGAGCTTTATGATATAAAACC
TAAAGATTTTGAAAAGATTATGCAAAAATTGGGCGCACAAGGCTTTGGAAAAAGTTTTTTTGTGTATAAGTTTAAAAATT
ATGATTTAGCTCTTGCTAGAACCGAAAACAAAATAAGCTATGGACATAAAGGTTTTGAAGTTCAAATTTGCAATGATGAA
AAACTTGGCGCCAAAAGACGCGATTTTACCATAAATTCTATGATGATTAATCTTTTTAACGATGAGTTTTTAGATTTTTA
TGGAGGATTAAAAGATCTAGAAGCAGGCTTTTTAAGGCATATTGATAAACAAAGCTTTCAAGAGGATAGTTTAAGAGTAT
TAAGAGCGGTAGTATTTGCTTCAAGGTTTAACTTTAAGATAACTAGCGAAAGCTTGAAATTGATGCAAAGTATGGATATA
ACTGATCTTAGCAAAGATAGGATCAATGCAGAGCTTTATAAATTCTTTAAAAGTCCTAGACTTGATGTCGGCTATAGATA
TTTACAAGAACTTGGCTTAGAGAAACAGGTTTTCGGCTTTGAGAGTGTTTTTAAGAGTTTGGAATTTCAAAATTTACTCA
GACAAAGTAGAGAATTTGTAAAAAGCGATGCTTTATTCTTGTATTTGTATTTGAATTTTTTTAATTTAGAAAAAGATATA
TTTTTTAAAAGAACTAAACTTAAAAAAGAATACCTAAAATATGCAAATCAAGCTTTTTATCTTGATGATATAAGTGATTT
TGAACTAGCAAAAATTGCTTTTGAAATGCCTTTGAAGGAATGGCTTGGACTTTGGAGTAAAAAGCGTATTGAGCAAGCTA
AAAGATTAGGGCTTTATGAGAATAAGTTTGAAAGTAAAATTTTAGCAAAAGATTTCATAAATGCTGGATTTTGTGGTAAA
ATTCTTGGCCTAAAGCTTCAAGAAGCAAGAGAGAATGAATTAAAAGAATATATAAAAGGTTTAGCTAAATGA

Upstream 100 bases:

>100_bases
TTAGAGTTTTTGCTTGCAAATATCGAAGATAAGCTTAAAAAATAATCCCGATTTACAGTTTATCGCTGAGTTTTTAAAGC
CTTATACTAAACGAGCGTAT

Downstream 100 bases:

>100_bases
TTTCTGTATTAAAAATTTGCACCAAAGATCAAAAAGGTTTAATCTATAGAATTTCTGATGTTATTTTTAAATATCATATC
AATATAGTAAAAAACGATGA

Product: polyA polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVGGSVRDLFLGLKICDYDIELYDIKPKDFEKIMQKLGAQGFGKSFFVYKFKNYDLALARTENKISYGHKGFEVQICNDE
KLGAKRRDFTINSMMINLFNDEFLDFYGGLKDLEAGFLRHIDKQSFQEDSLRVLRAVVFASRFNFKITSESLKLMQSMDI
TDLSKDRINAELYKFFKSPRLDVGYRYLQELGLEKQVFGFESVFKSLEFQNLLRQSREFVKSDALFLYLYLNFFNLEKDI
FFKRTKLKKEYLKYANQAFYLDDISDFELAKIAFEMPLKEWLGLWSKKRIEQAKRLGLYENKFESKILAKDFINAGFCGK
ILGLKLQEARENELKEYIKGLAK

Sequences:

>Translated_343_residues
MVGGSVRDLFLGLKICDYDIELYDIKPKDFEKIMQKLGAQGFGKSFFVYKFKNYDLALARTENKISYGHKGFEVQICNDE
KLGAKRRDFTINSMMINLFNDEFLDFYGGLKDLEAGFLRHIDKQSFQEDSLRVLRAVVFASRFNFKITSESLKLMQSMDI
TDLSKDRINAELYKFFKSPRLDVGYRYLQELGLEKQVFGFESVFKSLEFQNLLRQSREFVKSDALFLYLYLNFFNLEKDI
FFKRTKLKKEYLKYANQAFYLDDISDFELAKIAFEMPLKEWLGLWSKKRIEQAKRLGLYENKFESKILAKDFINAGFCGK
ILGLKLQEARENELKEYIKGLAK
>Mature_343_residues
MVGGSVRDLFLGLKICDYDIELYDIKPKDFEKIMQKLGAQGFGKSFFVYKFKNYDLALARTENKISYGHKGFEVQICNDE
KLGAKRRDFTINSMMINLFNDEFLDFYGGLKDLEAGFLRHIDKQSFQEDSLRVLRAVVFASRFNFKITSESLKLMQSMDI
TDLSKDRINAELYKFFKSPRLDVGYRYLQELGLEKQVFGFESVFKSLEFQNLLRQSREFVKSDALFLYLYLNFFNLEKDI
FFKRTKLKKEYLKYANQAFYLDDISDFELAKIAFEMPLKEWLGLWSKKRIEQAKRLGLYENKFESKILAKDFINAGFCGK
ILGLKLQEARENELKEYIKGLAK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=209, Percent_Identity=34.9282296650718, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081691, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=227, Percent_Identity=29.5154185022026, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 40416; Mature: 40416

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGGSVRDLFLGLKICDYDIELYDIKPKDFEKIMQKLGAQGFGKSFFVYKFKNYDLALAR
CCCCHHHHHHHHHEEEECCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEE
TENKISYGHKGFEVQICNDEKLGAKRRDFTINSMMINLFNDEFLDFYGGLKDLEAGFLRH
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IDKQSFQEDSLRVLRAVVFASRFNFKITSESLKLMQSMDITDLSKDRINAELYKFFKSPR
HHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
LDVGYRYLQELGLEKQVFGFESVFKSLEFQNLLRQSREFVKSDALFLYLYLNFFNLEKDI
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
FFKRTKLKKEYLKYANQAFYLDDISDFELAKIAFEMPLKEWLGLWSKKRIEQAKRLGLYE
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHH
NKFESKILAKDFINAGFCGKILGLKLQEARENELKEYIKGLAK
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVGGSVRDLFLGLKICDYDIELYDIKPKDFEKIMQKLGAQGFGKSFFVYKFKNYDLALAR
CCCCHHHHHHHHHEEEECCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEE
TENKISYGHKGFEVQICNDEKLGAKRRDFTINSMMINLFNDEFLDFYGGLKDLEAGFLRH
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IDKQSFQEDSLRVLRAVVFASRFNFKITSESLKLMQSMDITDLSKDRINAELYKFFKSPR
HHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
LDVGYRYLQELGLEKQVFGFESVFKSLEFQNLLRQSREFVKSDALFLYLYLNFFNLEKDI
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
FFKRTKLKKEYLKYANQAFYLDDISDFELAKIAFEMPLKEWLGLWSKKRIEQAKRLGLYE
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHH
NKFESKILAKDFINAGFCGKILGLKLQEARENELKEYIKGLAK
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA