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The map label for this gene is purU [H]

Identifier: 57237635

GI number: 57237635

Start: 818804

End: 819628

Strand: Direct

Name: purU [H]

Synonym: CJE0881

Alternate gene names: 57237635

Gene position: 818804-819628 (Clockwise)

Preceding gene: 57237634

Following gene: 57237637

Centisome position: 46.06

GC content: 29.33

Gene sequence:

>825_bases
ATGATTTCTGTATTAAAAATTTGCACCAAAGATCAAAAAGGTTTAATCTATAGAATTTCTGATGTTATTTTTAAATATCA
TATCAATATAGTAAAAAACGATGAATTTGTAGGCGAAGGAATGTTTTTTTTTCGTGCTCTTTTAGAAGGTGAGTTTGATA
AAGAAGCTTTTATAGGAACGCTTGAGGCTATGCTTGGACAAGAGGCCTTGATAGAGCTTTGCGAGAAGAGAAAAAAAGAT
ATTGTTGTTTTTGCTACTAAAGAAAGTCATTGTTTGGGAGATTTGCTAATAAAGCATTATAGTAACGAACTTGAAGCAAA
TATCAAAGCTGTGATTTCAAATCACAACTCTTTAAAGGATTTAGTTGAGAAATTTGAAATTCCTTATCATTTTATCAGCG
CTGAAAATTTAGATCGTAAAGAGCAAGAAAATCAGATTTTAAAATGTCTTGAACAATATAAATTTGATTATTTGGTTTTA
GCAAAATATATGCGAATTTTATCTCCTGATTTTGTTAGGCATTTTGAAGGCAAGATTATAAATATTCACCATTCTTTTTT
ACCTGCATTTATAGGAGCAAATCCTTATAAACAAGCCTTTGAAAGAGGGGTTAAAATAATTGGAGCTACGGCACATTTTG
TAAATAATAATCTTGATGAAGGTCCTATTATCACTCAAGCTGTTTCGCCTGTAAACCATGAATTTACTTGGCAAGATATG
CAGCAAGCAGGTAGAAATATAGAAAAAGATGTTCTTTCAAAAGCACTTGATTTGGCTTTTGAAGATAGAATTTTTATATA
TAATAATAAAACTATAATATTTTAA

Upstream 100 bases:

>100_bases
AAGATTTCATAAATGCTGGATTTTGTGGTAAAATTCTTGGCCTAAAGCTTCAAGAAGCAAGAGAGAATGAATTAAAAGAA
TATATAAAAGGTTTAGCTAA

Downstream 100 bases:

>100_bases
TGTGATAATTTTATTATAGTTTTATTTAAAGCATTAAAAAAATAATCAATATCCTCTTTTTCATGCGTATAATGCAAGCT
TATTCTTAACCAACCTGGTT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MISVLKICTKDQKGLIYRISDVIFKYHINIVKNDEFVGEGMFFFRALLEGEFDKEAFIGTLEAMLGQEALIELCEKRKKD
IVVFATKESHCLGDLLIKHYSNELEANIKAVISNHNSLKDLVEKFEIPYHFISAENLDRKEQENQILKCLEQYKFDYLVL
AKYMRILSPDFVRHFEGKIINIHHSFLPAFIGANPYKQAFERGVKIIGATAHFVNNNLDEGPIITQAVSPVNHEFTWQDM
QQAGRNIEKDVLSKALDLAFEDRIFIYNNKTIIF

Sequences:

>Translated_274_residues
MISVLKICTKDQKGLIYRISDVIFKYHINIVKNDEFVGEGMFFFRALLEGEFDKEAFIGTLEAMLGQEALIELCEKRKKD
IVVFATKESHCLGDLLIKHYSNELEANIKAVISNHNSLKDLVEKFEIPYHFISAENLDRKEQENQILKCLEQYKFDYLVL
AKYMRILSPDFVRHFEGKIINIHHSFLPAFIGANPYKQAFERGVKIIGATAHFVNNNLDEGPIITQAVSPVNHEFTWQDM
QQAGRNIEKDVLSKALDLAFEDRIFIYNNKTIIF
>Mature_274_residues
MISVLKICTKDQKGLIYRISDVIFKYHINIVKNDEFVGEGMFFFRALLEGEFDKEAFIGTLEAMLGQEALIELCEKRKKD
IVVFATKESHCLGDLLIKHYSNELEANIKAVISNHNSLKDLVEKFEIPYHFISAENLDRKEQENQILKCLEQYKFDYLVL
AKYMRILSPDFVRHFEGKIINIHHSFLPAFIGANPYKQAFERGVKIIGATAHFVNNNLDEGPIITQAVSPVNHEFTWQDM
QQAGRNIEKDVLSKALDLAFEDRIFIYNNKTIIF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1787483, Length=273, Percent_Identity=52.3809523809524, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1788846, Length=155, Percent_Identity=34.8387096774194, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=170, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582400, Length=182, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31712; Mature: 31712

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVLKICTKDQKGLIYRISDVIFKYHINIVKNDEFVGEGMFFFRALLEGEFDKEAFIGT
CCCHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
LEAMLGQEALIELCEKRKKDIVVFATKESHCLGDLLIKHYSNELEANIKAVISNHNSLKD
HHHHHCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVEKFEIPYHFISAENLDRKEQENQILKCLEQYKFDYLVLAKYMRILSPDFVRHFEGKII
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHCCCEE
NIHHSFLPAFIGANPYKQAFERGVKIIGATAHFVNNNLDEGPIITQAVSPVNHEFTWQDM
EEEHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHH
QQAGRNIEKDVLSKALDLAFEDRIFIYNNKTIIF
HHHCCCCHHHHHHHHHHHHHCCEEEEEECCEEEC
>Mature Secondary Structure
MISVLKICTKDQKGLIYRISDVIFKYHINIVKNDEFVGEGMFFFRALLEGEFDKEAFIGT
CCCHHHHHCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
LEAMLGQEALIELCEKRKKDIVVFATKESHCLGDLLIKHYSNELEANIKAVISNHNSLKD
HHHHHCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVEKFEIPYHFISAENLDRKEQENQILKCLEQYKFDYLVLAKYMRILSPDFVRHFEGKII
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHCCCEE
NIHHSFLPAFIGANPYKQAFERGVKIIGATAHFVNNNLDEGPIITQAVSPVNHEFTWQDM
EEEHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHH
QQAGRNIEKDVLSKALDLAFEDRIFIYNNKTIIF
HHHCCCCHHHHHHHHHHHHHCCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]