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The map label for this gene is proS [H]

Identifier: 21226809

GI number: 21226809

Start: 851567

End: 853009

Strand: Direct

Name: proS [H]

Synonym: MM_0707

Alternate gene names: 21226809

Gene position: 851567-853009 (Clockwise)

Preceding gene: 21226808

Following gene: 21226810

Centisome position: 20.79

GC content: 49.76

Gene sequence:

>1443_bases
ATGGCAGAAAGCGAAAAAGAAGCAGCACTTCCTCCAAAAGAGGCATTCAGCGACTGGTACAATGAACTCCTGTGGATGGC
AGAAATAATGGACGTCCGCTATCCTGTAAAAGGGCTTTACGTATGGTACCCGTTCGGCTTTGCCATCAGGAGGAATACCT
ACAGTATCATAAGGGAAATCCTTGACAACAGTGGACATCAGGAAACACTTTTTCCTCTACTTATCCCCGAAAACGAGTTC
ATGAAGGAAGCCGAGCACATCAAAGGCTTTGAGAACGAAGTTTACTGGGTCACACACGGAGGAAAAGACCCTCTTGACAT
ACCACTGGCTCTCCGCCCAACAAGTGAGACTGCCATATATCCGATGTATAAAAAATGGGTCAGGTCGCACGCTGACTTCC
CGCTGAAGCTATATCAGATAGTTAACACCTTCCGCTATGAGACAAAGCATACCCGGCCTCTTATCAGGCTCAGGGAGATC
ACTTCCTTCAAAGAAGCCCACACCGTACATGCCACCTGGGAAGATGCAGAAGCCCAGGTAAAGGAAGCTATCGGGCTTTA
CACTGAGATATACCGCAGGCTGGCAGTCCCTGTGCTTCGTTCAAGGAGACCTGACTGGGACAAGTTCCCTGGAGCGGACT
ATACCGATGCTCTTGATGCTGTTATGCCTGACGGGAAGACCCTTCAGATAGGGACTGTCCACCACCTGGGCGACAACTTT
GCAAAGACCTTTGATATAAAATACGAGGCTCCTGACGGCGAACAGCGCTATGCCCACCAGACCTGTTACGGGATTTCAGA
GAGGTCTATTGCAGCCACAATCTCCATTCATGGAGACGACAAGGGGCTCGTCCTTCCCCCGGAAATAGCACCTGTCCAGG
TAGTCATTATCCCCATCATCTTCAAGAAAGGAGCAGAAGAGGTTTTCGCAGCCTGCAAAGATGTTCAGGAACGCCTGAAG
AAAGCAGGGATCAGAGTAGAGGTCGATGCAAGTGACCTGCGCCCGGGAGCGAAATATTACAAATGGGAAATGAAAGGAGT
CCCTCTCAGGCTTGAAATTGGACCTCGTGACCTGCAAAATAATGTTGCGGTTGCAGTCAGGAGAGACACGGGAGAAAAGG
ACCAGATAACTCTGCTCGAGATAGAAGCCGGGGTTCGCCTGAAGTTTGAAGCAATCCAGAAAAGCCTTTACGAGAAAGCC
GGGTCTGAACTTGAAAGCCGCATATTTGACTGTGTCGATCTTGACGAGGTAAAAGAAAAGATCCAGGAAGGAGTTGCAAC
AATCCCCTGGTGCGGCAAGAGGGAATGCGGGCTTGCAATGGAAGACCATATAGGAGCAGGAATCCTGGGAATTCCCCTGA
CCCCGAGAAGTAAAGGAAAAGAAAAATGCCCTGCCTGCGGAGAGGAAACCGAAACTCGCGTTTACGTTGCAAGGACCTAT
TAA

Upstream 100 bases:

>100_bases
TACTTGATTGAAGCACTTCATAGTAGCATACTTGATTAAAACACTTCATAATTCGGTTTATCATAAGAACTAACTGAAAT
CACAATAATATGGTGAAGGT

Downstream 100 bases:

>100_bases
GACCTGTAAAAGCTCTTCTAAGACACTGGGCAAAATCTGGAAAGTCCAAGATCTGTATCCAGAAAACATCCTCCGGGTGT
TTTCTGCTTTTTTTATTTCC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAESEKEAALPPKEAFSDWYNELLWMAEIMDVRYPVKGLYVWYPFGFAIRRNTYSIIREILDNSGHQETLFPLLIPENEF
MKEAEHIKGFENEVYWVTHGGKDPLDIPLALRPTSETAIYPMYKKWVRSHADFPLKLYQIVNTFRYETKHTRPLIRLREI
TSFKEAHTVHATWEDAEAQVKEAIGLYTEIYRRLAVPVLRSRRPDWDKFPGADYTDALDAVMPDGKTLQIGTVHHLGDNF
AKTFDIKYEAPDGEQRYAHQTCYGISERSIAATISIHGDDKGLVLPPEIAPVQVVIIPIIFKKGAEEVFAACKDVQERLK
KAGIRVEVDASDLRPGAKYYKWEMKGVPLRLEIGPRDLQNNVAVAVRRDTGEKDQITLLEIEAGVRLKFEAIQKSLYEKA
GSELESRIFDCVDLDEVKEKIQEGVATIPWCGKRECGLAMEDHIGAGILGIPLTPRSKGKEKCPACGEETETRVYVARTY

Sequences:

>Translated_480_residues
MAESEKEAALPPKEAFSDWYNELLWMAEIMDVRYPVKGLYVWYPFGFAIRRNTYSIIREILDNSGHQETLFPLLIPENEF
MKEAEHIKGFENEVYWVTHGGKDPLDIPLALRPTSETAIYPMYKKWVRSHADFPLKLYQIVNTFRYETKHTRPLIRLREI
TSFKEAHTVHATWEDAEAQVKEAIGLYTEIYRRLAVPVLRSRRPDWDKFPGADYTDALDAVMPDGKTLQIGTVHHLGDNF
AKTFDIKYEAPDGEQRYAHQTCYGISERSIAATISIHGDDKGLVLPPEIAPVQVVIIPIIFKKGAEEVFAACKDVQERLK
KAGIRVEVDASDLRPGAKYYKWEMKGVPLRLEIGPRDLQNNVAVAVRRDTGEKDQITLLEIEAGVRLKFEAIQKSLYEKA
GSELESRIFDCVDLDEVKEKIQEGVATIPWCGKRECGLAMEDHIGAGILGIPLTPRSKGKEKCPACGEETETRVYVARTY
>Mature_479_residues
AESEKEAALPPKEAFSDWYNELLWMAEIMDVRYPVKGLYVWYPFGFAIRRNTYSIIREILDNSGHQETLFPLLIPENEFM
KEAEHIKGFENEVYWVTHGGKDPLDIPLALRPTSETAIYPMYKKWVRSHADFPLKLYQIVNTFRYETKHTRPLIRLREIT
SFKEAHTVHATWEDAEAQVKEAIGLYTEIYRRLAVPVLRSRRPDWDKFPGADYTDALDAVMPDGKTLQIGTVHHLGDNFA
KTFDIKYEAPDGEQRYAHQTCYGISERSIAATISIHGDDKGLVLPPEIAPVQVVIIPIIFKKGAEEVFAACKDVQERLKK
AGIRVEVDASDLRPGAKYYKWEMKGVPLRLEIGPRDLQNNVAVAVRRDTGEKDQITLLEIEAGVRLKFEAIQKSLYEKAG
SELESRIFDCVDLDEVKEKIQEGVATIPWCGKRECGLAMEDHIGAGILGIPLTPRSKGKEKCPACGEETETRVYVARTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=437, Percent_Identity=38.9016018306636, Blast_Score=325, Evalue=6e-89,
Organism=Escherichia coli, GI1786392, Length=162, Percent_Identity=30.2469135802469, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71991342, Length=487, Percent_Identity=37.3716632443532, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71991346, Length=354, Percent_Identity=41.5254237288136, Blast_Score=284, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6321808, Length=438, Percent_Identity=38.3561643835616, Blast_Score=303, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24649468, Length=511, Percent_Identity=35.812133072407, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24649466, Length=511, Percent_Identity=35.812133072407, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54706; Mature: 54575

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESEKEAALPPKEAFSDWYNELLWMAEIMDVRYPVKGLYVWYPFGFAIRRNTYSIIREI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCHHHHHHHH
LDNSGHQETLFPLLIPENEFMKEAEHIKGFENEVYWVTHGGKDPLDIPLALRPTSETAIY
HHCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEH
PMYKKWVRSHADFPLKLYQIVNTFRYETKHTRPLIRLREITSFKEAHTVHATWEDAEAQV
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHHH
KEAIGLYTEIYRRLAVPVLRSRRPDWDKFPGADYTDALDAVMPDGKTLQIGTVHHLGDNF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHCCCC
AKTFDIKYEAPDGEQRYAHQTCYGISERSIAATISIHGDDKGLVLPPEIAPVQVVIIPII
CEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCEEEEEEEEH
FKKGAEEVFAACKDVQERLKKAGIRVEVDASDLRPGAKYYKWEMKGVPLRLEIGPRDLQN
HHCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCCCCC
NVAVAVRRDTGEKDQITLLEIEAGVRLKFEAIQKSLYEKAGSELESRIFDCVDLDEVKEK
CEEEEEECCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHH
IQEGVATIPWCGKRECGLAMEDHIGAGILGIPLTPRSKGKEKCPACGEETETRVYVARTY
HHCCCEECCCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
AESEKEAALPPKEAFSDWYNELLWMAEIMDVRYPVKGLYVWYPFGFAIRRNTYSIIREI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCHHHHHHHH
LDNSGHQETLFPLLIPENEFMKEAEHIKGFENEVYWVTHGGKDPLDIPLALRPTSETAIY
HHCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEH
PMYKKWVRSHADFPLKLYQIVNTFRYETKHTRPLIRLREITSFKEAHTVHATWEDAEAQV
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHHH
KEAIGLYTEIYRRLAVPVLRSRRPDWDKFPGADYTDALDAVMPDGKTLQIGTVHHLGDNF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHCCCC
AKTFDIKYEAPDGEQRYAHQTCYGISERSIAATISIHGDDKGLVLPPEIAPVQVVIIPII
CEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCEEEEEEEEH
FKKGAEEVFAACKDVQERLKKAGIRVEVDASDLRPGAKYYKWEMKGVPLRLEIGPRDLQN
HHCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCCCCC
NVAVAVRRDTGEKDQITLLEIEAGVRLKFEAIQKSLYEKAGSELESRIFDCVDLDEVKEK
CEEEEEECCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHH
IQEGVATIPWCGKRECGLAMEDHIGAGILGIPLTPRSKGKEKCPACGEETETRVYVARTY
HHCCCEECCCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]