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The map label for this gene is frvX [C]

Identifier: 21226775

GI number: 21226775

Start: 808943

End: 810028

Strand: Reverse

Name: frvX [C]

Synonym: MM_0673

Alternate gene names: 21226775

Gene position: 810028-808943 (Counterclockwise)

Preceding gene: 21226782

Following gene: 21226774

Centisome position: 19.77

GC content: 47.79

Gene sequence:

>1086_bases
ATGGAAAAAAATACGGAAAAAGGCGAAAAGCTTGAAGAAATAAGATCCCTTCTCGAAAAGTTCACGAATGCTCACGGAAT
TTCCGGCTTTGAAGGCGAAGTCCGGAAACTGCTTGAGAAAGAGCTTGAGCCCTATGTTGACAGTATGCGGGAGGACATTA
TGGGGAACCTCATCGCTGTTAAAGAGGGCAAAGGTCCTTCTGTAATGCTGGCTGCTCATATGGATGAAATCGGGCTGATG
GTCAGATATATTGATGAGAACGGTTTCCTGCGCTTTGTCGGGATTGGGGGATGGTTTGACCAGACTCTTCTGAACCAGAG
AGTTATCGTGCATGGCAAAAAGGGCTCCATTTATGGAGTCATAGGGTCCAAACCTCCTCATGTGATGAAAGATGAGGACA
GAAAAAAGCCCGTGAAACTCGAGGACATGTTTATCGATATAGGGGCAAAGGACAGGGAGGACGCTGAAAATCTGGGTGTC
GAGATAGGTACTTCAGTATCAATCGATCAGGAATTCACGACCCTGGCAAACGGAAAGGTTACCTCCAAGGCTTTTGATAA
CCGTGCAGGAGTTGTAATTCTCGTCAGAGTTATGAAAGAGCTTCATGAACGCGGGATTGAAGCAAATGTCTATGCTGTCG
GCACGGTTCAGGAGGAAGTAGGGCTTAAAGGAGCAAAAACCTGTACTTTCTGCATCTGCCCGGATGTTGCCCTTGCCCTT
GACACCACCATCCCTGGAGATCATCCGGGAATCAGCAAAATCGATTCAGCCCTGGAACTTGGAAAAGGACCGGTGATAAC
TGTAGCAGACGCGTCCGGAAGAGGTCTCATAGCCCATCCGCAGATTCTCAAATGGCTCCGGGAAACCGCCGATAAAAATG
AGGTCCCCTATCAGCTGGGTGTAGGTTCTGGAGGCACCACAGACGCTACATCAATCCACCTCACCAAAGAAGGTATCCCT
ACAGGTACGGTCAGTATAGCCACACGCTACATTCACTCTCCTGTAGAGGTTCTGGATATGGCTGATATAGACGCCTGCGT
TTCTCTCATTGTGACGGCAATAGAAAATTTAAAAGCATACTTCTAA

Upstream 100 bases:

>100_bases
TAAAGCCGATAAACTATCCACAGTACTAACCTGTAATGTATTAACCTGTGTTCTTCAAATTGACATTCCAGATTTAAACA
GGATTCAGGAGATTTGTTAA

Downstream 100 bases:

>100_bases
AGCTACCGATTTTCAAGCCTGGCAAGTTTCTCCGGCTTTGCTCGCCCAAAGAGGCGATCTCCAGAGCCGGGGCGGCTGCA
CCGGAACAGCACCAGTAGAG

Product: hypothetical protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MEKNTEKGEKLEEIRSLLEKFTNAHGISGFEGEVRKLLEKELEPYVDSMREDIMGNLIAVKEGKGPSVMLAAHMDEIGLM
VRYIDENGFLRFVGIGGWFDQTLLNQRVIVHGKKGSIYGVIGSKPPHVMKDEDRKKPVKLEDMFIDIGAKDREDAENLGV
EIGTSVSIDQEFTTLANGKVTSKAFDNRAGVVILVRVMKELHERGIEANVYAVGTVQEEVGLKGAKTCTFCICPDVALAL
DTTIPGDHPGISKIDSALELGKGPVITVADASGRGLIAHPQILKWLRETADKNEVPYQLGVGSGGTTDATSIHLTKEGIP
TGTVSIATRYIHSPVEVLDMADIDACVSLIVTAIENLKAYF

Sequences:

>Translated_361_residues
MEKNTEKGEKLEEIRSLLEKFTNAHGISGFEGEVRKLLEKELEPYVDSMREDIMGNLIAVKEGKGPSVMLAAHMDEIGLM
VRYIDENGFLRFVGIGGWFDQTLLNQRVIVHGKKGSIYGVIGSKPPHVMKDEDRKKPVKLEDMFIDIGAKDREDAENLGV
EIGTSVSIDQEFTTLANGKVTSKAFDNRAGVVILVRVMKELHERGIEANVYAVGTVQEEVGLKGAKTCTFCICPDVALAL
DTTIPGDHPGISKIDSALELGKGPVITVADASGRGLIAHPQILKWLRETADKNEVPYQLGVGSGGTTDATSIHLTKEGIP
TGTVSIATRYIHSPVEVLDMADIDACVSLIVTAIENLKAYF
>Mature_361_residues
MEKNTEKGEKLEEIRSLLEKFTNAHGISGFEGEVRKLLEKELEPYVDSMREDIMGNLIAVKEGKGPSVMLAAHMDEIGLM
VRYIDENGFLRFVGIGGWFDQTLLNQRVIVHGKKGSIYGVIGSKPPHVMKDEDRKKPVKLEDMFIDIGAKDREDAENLGV
EIGTSVSIDQEFTTLANGKVTSKAFDNRAGVVILVRVMKELHERGIEANVYAVGTVQEEVGLKGAKTCTFCICPDVALAL
DTTIPGDHPGISKIDSALELGKGPVITVADASGRGLIAHPQILKWLRETADKNEVPYQLGVGSGGTTDATSIHLTKEGIP
TGTVSIATRYIHSPVEVLDMADIDACVSLIVTAIENLKAYF

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=344, Percent_Identity=39.2441860465116, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI87082417, Length=355, Percent_Identity=34.3661971830986, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1788727, Length=344, Percent_Identity=33.7209302325581, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 39231; Mature: 39231

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNTEKGEKLEEIRSLLEKFTNAHGISGFEGEVRKLLEKELEPYVDSMREDIMGNLIAV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
KEGKGPSVMLAAHMDEIGLMVRYIDENGFLRFVGIGGWFDQTLLNQRVIVHGKKGSIYGV
ECCCCCEEEEEECHHHHCEEEEEECCCCCEEEEECCCHHHHHHHCCEEEEECCCCCEEEE
IGSKPPHVMKDEDRKKPVKLEDMFIDIGAKDREDAENLGVEIGTSVSIDQEFTTLANGKV
ECCCCCCCCCCCCCCCCCEEEHEEEECCCCCCCCHHHCCEEECCCEECCCHHHHHCCCCE
TSKAFDNRAGVVILVRVMKELHERGIEANVYAVGTVQEEVGLKGAKTCTFCICPDVALAL
EHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHCCCCCCEEEEEECCCCEEEE
DTTIPGDHPGISKIDSALELGKGPVITVADASGRGLIAHPQILKWLRETADKNEVPYQLG
ECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCCEEEE
VGSGGTTDATSIHLTKEGIPTGTVSIATRYIHSPVEVLDMADIDACVSLIVTAIENLKAY
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
F
C
>Mature Secondary Structure
MEKNTEKGEKLEEIRSLLEKFTNAHGISGFEGEVRKLLEKELEPYVDSMREDIMGNLIAV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
KEGKGPSVMLAAHMDEIGLMVRYIDENGFLRFVGIGGWFDQTLLNQRVIVHGKKGSIYGV
ECCCCCEEEEEECHHHHCEEEEEECCCCCEEEEECCCHHHHHHHCCEEEEECCCCCEEEE
IGSKPPHVMKDEDRKKPVKLEDMFIDIGAKDREDAENLGVEIGTSVSIDQEFTTLANGKV
ECCCCCCCCCCCCCCCCCEEEHEEEECCCCCCCCHHHCCEEECCCEECCCHHHHHCCCCE
TSKAFDNRAGVVILVRVMKELHERGIEANVYAVGTVQEEVGLKGAKTCTFCICPDVALAL
EHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHCCCCCCEEEEEECCCCEEEE
DTTIPGDHPGISKIDSALELGKGPVITVADASGRGLIAHPQILKWLRETADKNEVPYQLG
ECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCCEEEE
VGSGGTTDATSIHLTKEGIPTGTVSIATRYIHSPVEVLDMADIDACVSLIVTAIENLKAY
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]