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The map label for this gene is aegA [H]

Identifier: 21226766

GI number: 21226766

Start: 795363

End: 796772

Strand: Direct

Name: aegA [H]

Synonym: MM_0664

Alternate gene names: 21226766

Gene position: 795363-796772 (Clockwise)

Preceding gene: 21226765

Following gene: 21226768

Centisome position: 19.42

GC content: 52.27

Gene sequence:

>1410_bases
ATGCAAGAATTACACGGAGAAAAGACAGGAAAAGCAAAGAAAGAGAGAACCCCAATGCCAGAGCAGCCTGCAGGGGAACG
CAGGAAGAACTTCAATGAAGTTGCCCTCGGCTACACGAAAGAAGATGCCCTTGCAGAGGCTTCCAGGTGTCTTGCCTGCA
AAGAACCGAAATGTGTTGAAGGCTGCCCTGTGAACGTGGATATTCCGGGATTTATTCAGCTTATATGTGAGGAGAATTTT
GAAGGGGCAATTGAAAGGATTAAAGCCACAAACGCTCTCCCTGCAATCTGCGGCCGCGTCTGCCCTCAGGAAAGCCAGTG
CGAAGCCCACTGTGTACTGGGGAAAAAGGGCCAGCCTGTTGCAATCGGAAGGCTTGAGCGTTTCTGTGCAGATTATGAGC
GCGAAAAAGGTGTGAAGTCTCCACAGGTCATGCCGCCCACAGGCAAGAGGGTGGCAGTTGTAGGTTCCGGTCCTGCAGGA
CTTACTGCCGCAGCAGACCTGGCAAAACTCGGGCACAAAGTAACAATCTTTGAGTCCCTGCACAAAGCCGGAGGAGTCCT
GAGTTACGGCATCCCCGAGTTCAGGCTGCCAAAAGAAATTGTCCGGCAGGAAATCGAGTACATTGAAAAACTCGGTGTTG
AGGTCAGGCCCAACTATATCATAGGCAGGATCAAGACTCTTGACGAATTATGTGACGATTTCGATGCCGTTTTCCTTGGA
ACCGGGGCAGGGCTCCCGAGCTTCATGGGAATAGAGGGCGAAAACCTGAATGGCGTATATTCAGCTAACGAATTCCTGAC
CCGGGTAAACCTCATGAAAGCCTATGACCCCGAATACGATACCACGGTCAGGCGCGGGAAGAATGTTGTGGTTGTAGGAG
GCGGAAATGTGGCAATGGACGCAGCCCGCTCTGCTCTCCGCCTGGGCGCAGACGAGGTAAGCATAGTCTACCGCCGCGGA
GAGGATGAGATGCCCGCCCGCCGTGAGGAGATCGAGCACGCCAAAGAAGAGGGCATTACTTTCAGGCTCCTTACAAACCC
CGTACGCATCCTTGGTGACGAGAAGTTCAACGTCACAGGTGTCGAATGCATAAGGATGGAACTTGGCGAACCCGATAAAT
CTGGCAGGAGAAGCCCTGTCCCTGTTGAAGGCTCGGAATTCCTTGTGCCGGCAGATGTTGTGGTTATTGCGATAGGCACC
TCTCCAAACCCGATCATTTTTAAAGGCTCGGAAGGGCTTGAACAGAATAAGAGAGGAACTGTAGTTGCTGATGAGGAGAC
CGGAGCGACCTCCAAATGTGGAGTCTTTGCCGGCGGAGACGTGGTTACAGGGGCAGCAACCGTTATCAGCGCAATGGGCG
CAGGTAAGAAGGCTGCAAAAGCAATTGATGAATATTTGAAAGAAAAGTGA

Upstream 100 bases:

>100_bases
GAACAGGCTTGCTATGTACCGCGGCGAAGAAACAGAAGCAACGGAAAAGTATGAAGAGAAATGCAGGTGCGGACTGCACT
GAACTGGAGGGGAAGAAAAA

Downstream 100 bases:

>100_bases
AACCAGAGAAATAAAGAAAAGTAGTAATGAAATAGCAGTGAGATAAAAGAAAGATGAAACTTCTTCATTTATTATTCTTT
TATCTCAGTTTCTTTGTATT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQELHGEKTGKAKKERTPMPEQPAGERRKNFNEVALGYTKEDALAEASRCLACKEPKCVEGCPVNVDIPGFIQLICEENF
EGAIERIKATNALPAICGRVCPQESQCEAHCVLGKKGQPVAIGRLERFCADYEREKGVKSPQVMPPTGKRVAVVGSGPAG
LTAAADLAKLGHKVTIFESLHKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDDFDAVFLG
TGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVGGGNVAMDAARSALRLGADEVSIVYRRG
EDEMPARREEIEHAKEEGITFRLLTNPVRILGDEKFNVTGVECIRMELGEPDKSGRRSPVPVEGSEFLVPADVVVIAIGT
SPNPIIFKGSEGLEQNKRGTVVADEETGATSKCGVFAGGDVVTGAATVISAMGAGKKAAKAIDEYLKEK

Sequences:

>Translated_469_residues
MQELHGEKTGKAKKERTPMPEQPAGERRKNFNEVALGYTKEDALAEASRCLACKEPKCVEGCPVNVDIPGFIQLICEENF
EGAIERIKATNALPAICGRVCPQESQCEAHCVLGKKGQPVAIGRLERFCADYEREKGVKSPQVMPPTGKRVAVVGSGPAG
LTAAADLAKLGHKVTIFESLHKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDDFDAVFLG
TGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVGGGNVAMDAARSALRLGADEVSIVYRRG
EDEMPARREEIEHAKEEGITFRLLTNPVRILGDEKFNVTGVECIRMELGEPDKSGRRSPVPVEGSEFLVPADVVVIAIGT
SPNPIIFKGSEGLEQNKRGTVVADEETGATSKCGVFAGGDVVTGAATVISAMGAGKKAAKAIDEYLKEK
>Mature_469_residues
MQELHGEKTGKAKKERTPMPEQPAGERRKNFNEVALGYTKEDALAEASRCLACKEPKCVEGCPVNVDIPGFIQLICEENF
EGAIERIKATNALPAICGRVCPQESQCEAHCVLGKKGQPVAIGRLERFCADYEREKGVKSPQVMPPTGKRVAVVGSGPAG
LTAAADLAKLGHKVTIFESLHKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDDFDAVFLG
TGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVGGGNVAMDAARSALRLGADEVSIVYRRG
EDEMPARREEIEHAKEEGITFRLLTNPVRILGDEKFNVTGVECIRMELGEPDKSGRRSPVPVEGSEFLVPADVVVIAIGT
SPNPIIFKGSEGLEQNKRGTVVADEETGATSKCGVFAGGDVVTGAATVISAMGAGKKAAKAIDEYLKEK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=473, Percent_Identity=32.9809725158562, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI237757300, Length=126, Percent_Identity=34.9206349206349, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788811, Length=457, Percent_Identity=40.0437636761488, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1789606, Length=460, Percent_Identity=38.2608695652174, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=38.8888888888889, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI1788468, Length=453, Percent_Identity=34.4370860927152, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1789243, Length=418, Percent_Identity=35.8851674641148, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=29.0060851926978, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71984108, Length=447, Percent_Identity=32.662192393736, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17543860, Length=159, Percent_Identity=35.8490566037736, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=467, Percent_Identity=29.7644539614561, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI28574881, Length=478, Percent_Identity=32.0083682008368, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24665539, Length=478, Percent_Identity=32.0083682008368, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24665547, Length=478, Percent_Identity=32.0083682008368, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24665543, Length=478, Percent_Identity=32.0083682008368, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24640763, Length=442, Percent_Identity=32.8054298642534, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI18858217, Length=442, Percent_Identity=32.8054298642534, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50566; Mature: 50566

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELHGEKTGKAKKERTPMPEQPAGERRKNFNEVALGYTKEDALAEASRCLACKEPKCVE
CCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCHHEECCCCHHHHHHHHHHHCCCCCCCCC
GCPVNVDIPGFIQLICEENFEGAIERIKATNALPAICGRVCPQESQCEAHCVLGKKGQPV
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
AIGRLERFCADYEREKGVKSPQVMPPTGKRVAVVGSGPAGLTAAADLAKLGHKVTIFESL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEHHHHHH
HKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDDFDAVFLG
HHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHCCCCEEEEE
TGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVGGGNVAMD
CCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCHHHH
AARSALRLGADEVSIVYRRGEDEMPARREEIEHAKEEGITFRLLTNPVRILGDEKFNVTG
HHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHEECCCCCCCCH
VECIRMELGEPDKSGRRSPVPVEGSEFLVPADVVVIAIGTSPNPIIFKGSEGLEQNKRGT
HEEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCC
VVADEETGATSKCGVFAGGDVVTGAATVISAMGAGKKAAKAIDEYLKEK
EEECCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQELHGEKTGKAKKERTPMPEQPAGERRKNFNEVALGYTKEDALAEASRCLACKEPKCVE
CCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCHHEECCCCHHHHHHHHHHHCCCCCCCCC
GCPVNVDIPGFIQLICEENFEGAIERIKATNALPAICGRVCPQESQCEAHCVLGKKGQPV
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
AIGRLERFCADYEREKGVKSPQVMPPTGKRVAVVGSGPAGLTAAADLAKLGHKVTIFESL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEHHHHHH
HKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDDFDAVFLG
HHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEHHHHHHHHHHCCCCEEEEE
TGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVGGGNVAMD
CCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCCCHHHH
AARSALRLGADEVSIVYRRGEDEMPARREEIEHAKEEGITFRLLTNPVRILGDEKFNVTG
HHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHEECCCCCCCCH
VECIRMELGEPDKSGRRSPVPVEGSEFLVPADVVVIAIGTSPNPIIFKGSEGLEQNKRGT
HEEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCC
VVADEETGATSKCGVFAGGDVVTGAATVISAMGAGKKAAKAIDEYLKEK
EEECCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]