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The map label for this gene is fcl [H]

Identifier: 21226760

GI number: 21226760

Start: 788387

End: 789325

Strand: Reverse

Name: fcl [H]

Synonym: MM_0658

Alternate gene names: 21226760

Gene position: 789325-788387 (Counterclockwise)

Preceding gene: 21226761

Following gene: 21226759

Centisome position: 19.27

GC content: 38.34

Gene sequence:

>939_bases
ATGGATAAAAGGTCAAAAATTTATGTGGCTGGCCACCGAGGTCTTGTAGGTTCAGCTCTGAAAAGAAAACTTGAGTCAAA
AGATTATTCAAATCTTATCTTCCGAACTCATCGAGAGCTTGATCTCACTAATCAGCAGGCTGTTAATGAATTTTTTGAAC
GAGAAAAGCCGGAGTATGTTTTTTTAGCCGCTGCAAAAGTTGGTGGAATTCTTGCCAATAATACTTATCCTGCCGAGTTC
ATTTACGAAAATCTTATGATCGAAGCAAATATTATTCACGCTTCCTACAAGTGCGGCGTAAAAAAACTACTTTTTCTGGG
TTCCTCCTGTATTTATCCTAAACTCGCTCCCCAACCTCTCAAAGAAGAATATCTATTGACCGGCCCTCTTGAAGAAACAA
ATGAAGCATATGCAGTTGCAAAGATCGCAGGAATAAGGCTTTGCAAACACTATAACCAGCAGTATGGGACTAACTTCATA
TCGGTAATGCCGACCAATCTTTATGGGCCTAATGATAATTTTGACCTTGAGACTTCACATGTAATGCCTGCATTGGTAAG
GAAGTTCCATGAAGCCAAGGTGAATAATGAGCCTGAAGTTGTAATCTGGGGTACCGGTAAACCATACAGGGAGTTTCTTC
ACGTAGATGATATGGCTGACGCCTGTGTGTATTTAATGGAAAACTTTAATACGGATGACATTGGAGAATTTGTCAATATC
GGGGTAGGGAAAGATATTACAATAGGTGAATTAGCTGAATTAATCAAAGAGATCGTAGGGTTTAAAGGTGAAATTAGAAA
AGATCTTTCAAAGCCTGACGGTACTCCACAGAAGCTGCTTGATATTACAAAATTGAGCTCACTTGGGTGGAAAGCTAATA
TCTCATTAAAAGATGGAATTAGACAAACTTATGAATGGTATCAAAGTCAAATTAAATAA

Upstream 100 bases:

>100_bases
CAAAAGTAAAACTGGAAGAGCTGGTAAAAATCATGATAACGGCCGACGAGAAAGCTGTACAGGAAAATCACACTTTTGAG
TAAAGTTCAATGGTGATCAC

Downstream 100 bases:

>100_bases
AGATATTAAAAATATAAATGAAGTGAAAAGCTTAGTAAATAAAAGGCTTGAAATATTTATTAGCTGTGCAATAGAGCAGA
ATGTATCCACAGCTAAAAAC

Product: GDP-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDKRSKIYVAGHRGLVGSALKRKLESKDYSNLIFRTHRELDLTNQQAVNEFFEREKPEYVFLAAAKVGGILANNTYPAEF
IYENLMIEANIIHASYKCGVKKLLFLGSSCIYPKLAPQPLKEEYLLTGPLEETNEAYAVAKIAGIRLCKHYNQQYGTNFI
SVMPTNLYGPNDNFDLETSHVMPALVRKFHEAKVNNEPEVVIWGTGKPYREFLHVDDMADACVYLMENFNTDDIGEFVNI
GVGKDITIGELAELIKEIVGFKGEIRKDLSKPDGTPQKLLDITKLSSLGWKANISLKDGIRQTYEWYQSQIK

Sequences:

>Translated_312_residues
MDKRSKIYVAGHRGLVGSALKRKLESKDYSNLIFRTHRELDLTNQQAVNEFFEREKPEYVFLAAAKVGGILANNTYPAEF
IYENLMIEANIIHASYKCGVKKLLFLGSSCIYPKLAPQPLKEEYLLTGPLEETNEAYAVAKIAGIRLCKHYNQQYGTNFI
SVMPTNLYGPNDNFDLETSHVMPALVRKFHEAKVNNEPEVVIWGTGKPYREFLHVDDMADACVYLMENFNTDDIGEFVNI
GVGKDITIGELAELIKEIVGFKGEIRKDLSKPDGTPQKLLDITKLSSLGWKANISLKDGIRQTYEWYQSQIK
>Mature_312_residues
MDKRSKIYVAGHRGLVGSALKRKLESKDYSNLIFRTHRELDLTNQQAVNEFFEREKPEYVFLAAAKVGGILANNTYPAEF
IYENLMIEANIIHASYKCGVKKLLFLGSSCIYPKLAPQPLKEEYLLTGPLEETNEAYAVAKIAGIRLCKHYNQQYGTNFI
SVMPTNLYGPNDNFDLETSHVMPALVRKFHEAKVNNEPEVVIWGTGKPYREFLHVDDMADACVYLMENFNTDDIGEFVNI
GVGKDITIGELAELIKEIVGFKGEIRKDLSKPDGTPQKLLDITKLSSLGWKANISLKDGIRQTYEWYQSQIK

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=310, Percent_Identity=31.2903225806452, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI7657641, Length=276, Percent_Identity=24.6376811594203, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI42516563, Length=329, Percent_Identity=24.9240121580547, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788365, Length=311, Percent_Identity=57.556270096463, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=25.1412429378531, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=306, Percent_Identity=29.4117647058824, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=24.5508982035928, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922778, Length=312, Percent_Identity=31.0897435897436, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=25.2279635258359, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35384; Mature: 35384

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRSKIYVAGHRGLVGSALKRKLESKDYSNLIFRTHRELDLTNQQAVNEFFEREKPEYV
CCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEE
FLAAAKVGGILANNTYPAEFIYENLMIEANIIHASYKCGVKKLLFLGSSCIYPKLAPQPL
EEEEHHHCCEEECCCCCHHHHHHHHEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEEYLLTGPLEETNEAYAVAKIAGIRLCKHYNQQYGTNFISVMPTNLYGPNDNFDLETSH
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VMPALVRKFHEAKVNNEPEVVIWGTGKPYREFLHVDDMADACVYLMENFNTDDIGEFVNI
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GVGKDITIGELAELIKEIVGFKGEIRKDLSKPDGTPQKLLDITKLSSLGWKANISLKDGI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCHHHHH
RQTYEWYQSQIK
HHHHHHHHHHCC
>Mature Secondary Structure
MDKRSKIYVAGHRGLVGSALKRKLESKDYSNLIFRTHRELDLTNQQAVNEFFEREKPEYV
CCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEE
FLAAAKVGGILANNTYPAEFIYENLMIEANIIHASYKCGVKKLLFLGSSCIYPKLAPQPL
EEEEHHHCCEEECCCCCHHHHHHHHEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEEYLLTGPLEETNEAYAVAKIAGIRLCKHYNQQYGTNFISVMPTNLYGPNDNFDLETSH
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VMPALVRKFHEAKVNNEPEVVIWGTGKPYREFLHVDDMADACVYLMENFNTDDIGEFVNI
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GVGKDITIGELAELIKEIVGFKGEIRKDLSKPDGTPQKLLDITKLSSLGWKANISLKDGI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCHHHHH
RQTYEWYQSQIK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]