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The map label for this gene is pgk [H]

Identifier: 21226587

GI number: 21226587

Start: 608492

End: 609742

Strand: Reverse

Name: pgk [H]

Synonym: MM_0485

Alternate gene names: 21226587

Gene position: 609742-608492 (Counterclockwise)

Preceding gene: 21226592

Following gene: 21226586

Centisome position: 14.89

GC content: 46.84

Gene sequence:

>1251_bases
GTGCTGAGGGTAATGACTTCCAGAAACTTTCTTACGATCGATGATTTTGACATACGCGGAAAGACGATTCTTTTAAGGGT
CGACATGAACTCTCCTATGGACACTCAGGGTCACATTCTGGACGATATGAGGATCAGGAGTCATATTGCCACTCTTAAAG
ACCTTGAGAGTGCAAAAGTCGTTGTGCTTGCGCACCAGAGCCGGCCTGGGAAAAAAGACTTCACCACTATGAAACCTCAT
GCTCACCTGCTGTCCAGGTATCTGGGCAGGCAAGTTACTTATGTGGACGACATCTTCGGGACTTTTGCGAAGACTCAGAT
CGCTTCTATGGAAGACGGGGACGTCATCATGCTCGAAAACGTCAGGTTCTATTCTGAAGAGAGCCTCGAGAGGACTACAG
CCGAGCAGGCCAATACTTTTATGGTTAAAAAACTGGCACCCTTTGTTGATATTTTCCTTAATGACGCGTTTGCGGTATCT
CACAGGTCTCATCTCTCTGTTGTAGGCTTTACCGAAGTCCTCCCTTCCGGAGCAGGCAGGGTAATGGAAAAAGAACTTAT
TTCCCTGGAAAAAGGAGTTAAAAGTGGAGAGAGGCCAAGCGTTTTCGTGCTGGGGGGGGCAAAAGTGGACGACTCACTTA
GAGTAACCGAAAATGTCCTCACAAACGGGGGAGCTGACAGAGTCCTCCTTACAGGGGTAGTTGCAAACGTTGCCCTTGCC
GCTTCCGGAGTAAATATAGGAAAAGCCAATCTGGATTTCATTAAATCCCAGGGCTACGAAGACCAGATAGAAAGGGCGAG
AGGTTTGCTTGCGAAGTTCGAAGATAAAATCGGACTTCCGAAAGACGTTGCTTTAAATGATAACAAAAAGCGTGTTGAAG
CTCCTATTTCCGAACTTAATTCCGATTCTCTGCCCATTAACGATATAGGTCTTGAAACCATTGTGGACTACACCAATGAA
ATCCAGAATGCCAAAACAGTTGTCCTGAACGGCCCTGCCGGGGTTTCAGAGATTGAGGACTTTGCCCTCGGAACCCATGA
AATTATAAAAGCTGCTATTAAATCCGATTTCTCTATCATTGGCGGCGGGCATATCTCTGTGGAAGTTGCACACCTCGGGC
TTGAACACCGCTTTTCACACATAAGCACTGGAGGAGGAGCCTGTATTGATTTCCTTGCAGGGGAAAAGCTGCCAGGCGTC
GAAGCTCTCAAGGCTGCTTACAATAAGTATCAGGAAGCTAAAAAGCTTTAA

Upstream 100 bases:

>100_bases
TTCCATGACAAGATAACCAATACTGAAAGAATAAGATTCTCTTAAATTTTATTTATCAAGCTTTATTCTTCGATATATAT
AAAATTTTGTTTACAAAGGT

Downstream 100 bases:

>100_bases
AGGCCTGTTTCCTGCAGCTTCCGGCAATGCCAGAAACCTACTTTAACCAGCTTCGATAAACATACTGTTACACCGGCGCA
CAGACATACAAGTAAACAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLRVMTSRNFLTIDDFDIRGKTILLRVDMNSPMDTQGHILDDMRIRSHIATLKDLESAKVVVLAHQSRPGKKDFTTMKPH
AHLLSRYLGRQVTYVDDIFGTFAKTQIASMEDGDVIMLENVRFYSEESLERTTAEQANTFMVKKLAPFVDIFLNDAFAVS
HRSHLSVVGFTEVLPSGAGRVMEKELISLEKGVKSGERPSVFVLGGAKVDDSLRVTENVLTNGGADRVLLTGVVANVALA
ASGVNIGKANLDFIKSQGYEDQIERARGLLAKFEDKIGLPKDVALNDNKKRVEAPISELNSDSLPINDIGLETIVDYTNE
IQNAKTVVLNGPAGVSEIEDFALGTHEIIKAAIKSDFSIIGGGHISVEVAHLGLEHRFSHISTGGGACIDFLAGEKLPGV
EALKAAYNKYQEAKKL

Sequences:

>Translated_416_residues
MLRVMTSRNFLTIDDFDIRGKTILLRVDMNSPMDTQGHILDDMRIRSHIATLKDLESAKVVVLAHQSRPGKKDFTTMKPH
AHLLSRYLGRQVTYVDDIFGTFAKTQIASMEDGDVIMLENVRFYSEESLERTTAEQANTFMVKKLAPFVDIFLNDAFAVS
HRSHLSVVGFTEVLPSGAGRVMEKELISLEKGVKSGERPSVFVLGGAKVDDSLRVTENVLTNGGADRVLLTGVVANVALA
ASGVNIGKANLDFIKSQGYEDQIERARGLLAKFEDKIGLPKDVALNDNKKRVEAPISELNSDSLPINDIGLETIVDYTNE
IQNAKTVVLNGPAGVSEIEDFALGTHEIIKAAIKSDFSIIGGGHISVEVAHLGLEHRFSHISTGGGACIDFLAGEKLPGV
EALKAAYNKYQEAKKL
>Mature_416_residues
MLRVMTSRNFLTIDDFDIRGKTILLRVDMNSPMDTQGHILDDMRIRSHIATLKDLESAKVVVLAHQSRPGKKDFTTMKPH
AHLLSRYLGRQVTYVDDIFGTFAKTQIASMEDGDVIMLENVRFYSEESLERTTAEQANTFMVKKLAPFVDIFLNDAFAVS
HRSHLSVVGFTEVLPSGAGRVMEKELISLEKGVKSGERPSVFVLGGAKVDDSLRVTENVLTNGGADRVLLTGVVANVALA
ASGVNIGKANLDFIKSQGYEDQIERARGLLAKFEDKIGLPKDVALNDNKKRVEAPISELNSDSLPINDIGLETIVDYTNE
IQNAKTVVLNGPAGVSEIEDFALGTHEIIKAAIKSDFSIIGGGHISVEVAHLGLEHRFSHISTGGGACIDFLAGEKLPGV
EALKAAYNKYQEAKKL

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=426, Percent_Identity=33.8028169014084, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI31543397, Length=423, Percent_Identity=32.8605200945626, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1789294, Length=416, Percent_Identity=31.4903846153846, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17508823, Length=426, Percent_Identity=33.8028169014084, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319857, Length=421, Percent_Identity=31.8289786223278, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI17136394, Length=423, Percent_Identity=33.806146572104, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24581203, Length=419, Percent_Identity=31.0262529832936, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 45391; Mature: 45391

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVMTSRNFLTIDDFDIRGKTILLRVDMNSPMDTQGHILDDMRIRSHIATLKDLESAKV
CEEEEECCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VVLAHQSRPGKKDFTTMKPHAHLLSRYLGRQVTYVDDIFGTFAKTQIASMEDGDVIMLEN
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
VRFYSEESLERTTAEQANTFMVKKLAPFVDIFLNDAFAVSHRSHLSVVGFTEVLPSGAGR
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEEEEEHHHHCCCCCCH
VMEKELISLEKGVKSGERPSVFVLGGAKVDDSLRVTENVLTNGGADRVLLTGVVANVALA
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ASGVNIGKANLDFIKSQGYEDQIERARGLLAKFEDKIGLPKDVALNDNKKRVEAPISELN
HCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCHHHCC
SDSLPINDIGLETIVDYTNEIQNAKTVVLNGPAGVSEIEDFALGTHEIIKAAIKSDFSII
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEE
GGGHISVEVAHLGLEHRFSHISTGGGACIDFLAGEKLPGVEALKAAYNKYQEAKKL
ECCEEEEEEEECCHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRVMTSRNFLTIDDFDIRGKTILLRVDMNSPMDTQGHILDDMRIRSHIATLKDLESAKV
CEEEEECCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VVLAHQSRPGKKDFTTMKPHAHLLSRYLGRQVTYVDDIFGTFAKTQIASMEDGDVIMLEN
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
VRFYSEESLERTTAEQANTFMVKKLAPFVDIFLNDAFAVSHRSHLSVVGFTEVLPSGAGR
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEEEEEHHHHCCCCCCH
VMEKELISLEKGVKSGERPSVFVLGGAKVDDSLRVTENVLTNGGADRVLLTGVVANVALA
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ASGVNIGKANLDFIKSQGYEDQIERARGLLAKFEDKIGLPKDVALNDNKKRVEAPISELN
HCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCHHHCC
SDSLPINDIGLETIVDYTNEIQNAKTVVLNGPAGVSEIEDFALGTHEIIKAAIKSDFSII
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEE
GGGHISVEVAHLGLEHRFSHISTGGGACIDFLAGEKLPGVEALKAAYNKYQEAKKL
ECCEEEEEEEECCHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]