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The map label for this gene is purN [H]

Identifier: 21226545

GI number: 21226545

Start: 564066

End: 564674

Strand: Reverse

Name: purN [H]

Synonym: MM_0443

Alternate gene names: 21226545

Gene position: 564674-564066 (Counterclockwise)

Preceding gene: 21226546

Following gene: 21226544

Centisome position: 13.78

GC content: 47.78

Gene sequence:

>609_bases
ATGACGGTAAAGATTGCAGTGCTGGTCTCTGGACGGGGTTCAAATCTCCAGGCGATCATAGATAGCATTGAAAAGGGCTA
CATAAAAAACGCGGCAGTTAATGTAGTTATCTCCAACAAAGCCGATGCTTATGCGCTCGAACGTGCAAAAAACCACGGTA
TCAGTGCTGTCTTTCTTGACTCCAGAGGGCGGGACAGAGCTGAATATGACAGGGAAATCCTGAAAGTGCTCAGACAGTAT
GATACTGACCTCCTTTTGCTTGCAGGCTATTTCCGGCTTCTGGGAAGCGAGATTATTAATGCATACCGGAACAGGATACT
GAACATCCATCCCTCTCTTCTCCCGGCTTTCAAAGGGCTTCATGCCCAGAAACAGGCTTTTGAATATGGGGTAAAGGTTG
CCGGCTGCACTGTTCATTTTGTAGACGAAGGTCTCGATTCAGGTCCGATTATTATCCAGAGATGCGTGCCTGTGCTCCCG
GGAGATACCGAAGAGACCCTTACTGACAGGATTCTTGAGCAGGAGCATATTATCTATCCCGAAGCTGTCAGGCTTTTTGT
CGAGGGGAAACTTAAAGTTGAGGGCAGGAACGTAACAGCCCCTGATTAA

Upstream 100 bases:

>100_bases
GACAAACTATTTTTCTCTTCCTCTGGATTTACTTCCGTTTTTAAGGGTGCGGTAAAGCTCCAAAAACCTAATATATCACT
GTTTCAATCTAAATCCCTCA

Downstream 100 bases:

>100_bases
GACCTTTGAAAAGGAAGATAAAGGAACCCGGGATTTCATCAGTTTTATCATGAGGTGCAAGACTGGAGAGAATCAGACCC
TGAGCTGGAGTAAAGTTTTC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTVKIAVLVSGRGSNLQAIIDSIEKGYIKNAAVNVVISNKADAYALERAKNHGISAVFLDSRGRDRAEYDREILKVLRQY
DTDLLLLAGYFRLLGSEIINAYRNRILNIHPSLLPAFKGLHAQKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQRCVPVLP
GDTEETLTDRILEQEHIIYPEAVRLFVEGKLKVEGRNVTAPD

Sequences:

>Translated_202_residues
MTVKIAVLVSGRGSNLQAIIDSIEKGYIKNAAVNVVISNKADAYALERAKNHGISAVFLDSRGRDRAEYDREILKVLRQY
DTDLLLLAGYFRLLGSEIINAYRNRILNIHPSLLPAFKGLHAQKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQRCVPVLP
GDTEETLTDRILEQEHIIYPEAVRLFVEGKLKVEGRNVTAPD
>Mature_201_residues
TVKIAVLVSGRGSNLQAIIDSIEKGYIKNAAVNVVISNKADAYALERAKNHGISAVFLDSRGRDRAEYDREILKVLRQYD
TDLLLLAGYFRLLGSEIINAYRNRILNIHPSLLPAFKGLHAQKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQRCVPVLPG
DTEETLTDRILEQEHIIYPEAVRLFVEGKLKVEGRNVTAPD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=44.6153846153846, Blast_Score=170, Evalue=9e-43,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=44.6153846153846, Blast_Score=170, Evalue=9e-43,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=44.6153846153846, Blast_Score=170, Evalue=9e-43,
Organism=Homo sapiens, GI238814322, Length=152, Percent_Identity=33.5526315789474, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21614513, Length=152, Percent_Identity=35.5263157894737, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=47.1502590673575, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787483, Length=105, Percent_Identity=40.9523809523809, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=44.1988950276243, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=203, Percent_Identity=32.0197044334975, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=46.8085106382979, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22428; Mature: 22297

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKIAVLVSGRGSNLQAIIDSIEKGYIKNAAVNVVISNKADAYALERAKNHGISAVFLD
CEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEC
SRGRDRAEYDREILKVLRQYDTDLLLLAGYFRLLGSEIINAYRNRILNIHPSLLPAFKGL
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCC
HAQKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQRCVPVLPGDTEETLTDRILEQEHIIYP
HHHHHHHHHCCEEECEEEEEECCCCCCCCEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
EAVRLFVEGKLKVEGRNVTAPD
HHHEEEEECEEEECCCCCCCCC
>Mature Secondary Structure 
TVKIAVLVSGRGSNLQAIIDSIEKGYIKNAAVNVVISNKADAYALERAKNHGISAVFLD
EEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEC
SRGRDRAEYDREILKVLRQYDTDLLLLAGYFRLLGSEIINAYRNRILNIHPSLLPAFKGL
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCC
HAQKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQRCVPVLPGDTEETLTDRILEQEHIIYP
HHHHHHHHHCCEEECEEEEEECCCCCCCCEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
EAVRLFVEGKLKVEGRNVTAPD
HHHEEEEECEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]