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The map label for this gene is glyA [H]

Identifier: 21226544

GI number: 21226544

Start: 562270

End: 563529

Strand: Reverse

Name: glyA [H]

Synonym: MM_0442

Alternate gene names: 21226544

Gene position: 563529-562270 (Counterclockwise)

Preceding gene: 21226545

Following gene: 21226543

Centisome position: 13.76

GC content: 48.49

Gene sequence:

>1260_bases
ATGAGTTATAAGGTGACAGTGATGTCTTATATTGAAAAAATTGACCCGGAATTGTTCGAGGCTATCAAAAATGAAGCCGA
TCGTCAGGAACACAAGCTGAACCTGATCGCATCAGAAAACTATGCGAGCAGGGCTGTTATGGAAGCCCAGGGCTCTATTA
TGACCAACAAGTATGCCGAAGGATATTCCGGTAAACGCTATTACGGAGGCTGCGATTTCGTTGACGTTGCCGAAAATCTC
GCCATTGCAAGGGCAAAGGAACTTTTCGGGGCAAAGTATGTAAACGTCCAGCCTCACTCAGGTTCAGGTGCCAACATGGC
TGTTTATTTCTCTGTGCTGCAGCCGGGGGATACTATCCTGTCCATGGACCTCTCTCACGGCGGACACCTTTCTCACGGCA
GCCCTGTAAGTTTCTCAGGAAAACTCTATAACATCGTGCCCTATGGGGTCAGCAAAGAGACCGAAGCCCTGGATTACGAT
GAGCTCCTTAAACTGGCAAAAGAATGCAAGCCCAGGATGATAGTATGCGGAGCTTCAGCCTATCCCCGTGTGATTGATTT
TAAAAGGTTCAGGGAAATTGCTGATGAGGTCGGAGCTTACCTCCTTGCAGATATTGCACACATCGCAGGACTTGTAGTTG
CAGGCGTACACCCGAGCCCTGTTCCTTATGCGGATTTTGTTACCACAACCACCCACAAGACCCTGCGCGGTCCCAGGGGC
GGAATGATTATCTCGAAGACTGAAGAGCTTGCAATAGGCGTGAACAAAGCGGTTTTCCCGGGTATCCAGGGCGGCCCCCT
CATGCATATCATAGCTGCAAAAGCTGTTGCCTTCAAAGAAGCCATGAGCGAAGAGTTCAGGCAGGACCAGGATCAGACCG
TAAAGAACGCAAAGGTCCTGTGTTCATGTCTGAAGCAGAAAGGTTTTGATATTGTTTCAGGCGGCACCGACAACCACCTT
ATGCTTGTAAATCTCAATAATATGAATATTACAGGAAAGGACGCTGAAGCTGCACTGAGCAAAGCCGGGATCATCGCCAA
CAAAAACACAGTTCCTTTCGAGACCCGCAGCCCATTCGTAACCAGCGGGGTAAGGCTCGGGACCCCTTCCTGCACCACAA
GGGGCATGAAAGAAAAGGAAATGGAACTGGTTGCAGACTACATCGAGGCTGCAATAAAGAATTCGGAAAACGATGCTCTT
CTGTCGGAAATCAATATTAAGGTAAGGGATCTCTGTTTAAAGTTCCCTGTTTACGAATAA

Upstream 100 bases:

>100_bases
CTCTTTTAAATCCGGAAGGTTGTAAAACCTCCTGATAAATCTGCCTATGAAGAAATTCAATTAACTCTATCAAGAAACCC
TTAAAACAAGTTTTAAATGC

Downstream 100 bases:

>100_bases
TTGAGGAGATAGGGGGTTTACCATTTGTTAGATGAAAGCTATGAGTCACGTATCATAGATGGAAAAGTCCTTGCACAGCA
GGTCGAAGAAGAGGTAAGGT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSYKVTVMSYIEKIDPELFEAIKNEADRQEHKLNLIASENYASRAVMEAQGSIMTNKYAEGYSGKRYYGGCDFVDVAENL
AIARAKELFGAKYVNVQPHSGSGANMAVYFSVLQPGDTILSMDLSHGGHLSHGSPVSFSGKLYNIVPYGVSKETEALDYD
ELLKLAKECKPRMIVCGASAYPRVIDFKRFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTTTTHKTLRGPRG
GMIISKTEELAIGVNKAVFPGIQGGPLMHIIAAKAVAFKEAMSEEFRQDQDQTVKNAKVLCSCLKQKGFDIVSGGTDNHL
MLVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFVTSGVRLGTPSCTTRGMKEKEMELVADYIEAAIKNSENDAL
LSEINIKVRDLCLKFPVYE

Sequences:

>Translated_419_residues
MSYKVTVMSYIEKIDPELFEAIKNEADRQEHKLNLIASENYASRAVMEAQGSIMTNKYAEGYSGKRYYGGCDFVDVAENL
AIARAKELFGAKYVNVQPHSGSGANMAVYFSVLQPGDTILSMDLSHGGHLSHGSPVSFSGKLYNIVPYGVSKETEALDYD
ELLKLAKECKPRMIVCGASAYPRVIDFKRFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTTTTHKTLRGPRG
GMIISKTEELAIGVNKAVFPGIQGGPLMHIIAAKAVAFKEAMSEEFRQDQDQTVKNAKVLCSCLKQKGFDIVSGGTDNHL
MLVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFVTSGVRLGTPSCTTRGMKEKEMELVADYIEAAIKNSENDAL
LSEINIKVRDLCLKFPVYE
>Mature_418_residues
SYKVTVMSYIEKIDPELFEAIKNEADRQEHKLNLIASENYASRAVMEAQGSIMTNKYAEGYSGKRYYGGCDFVDVAENLA
IARAKELFGAKYVNVQPHSGSGANMAVYFSVLQPGDTILSMDLSHGGHLSHGSPVSFSGKLYNIVPYGVSKETEALDYDE
LLKLAKECKPRMIVCGASAYPRVIDFKRFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTTTTHKTLRGPRGG
MIISKTEELAIGVNKAVFPGIQGGPLMHIIAAKAVAFKEAMSEEFRQDQDQTVKNAKVLCSCLKQKGFDIVSGGTDNHLM
LVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFVTSGVRLGTPSCTTRGMKEKEMELVADYIEAAIKNSENDALL
SEINIKVRDLCLKFPVYE

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=447, Percent_Identity=43.8478747203579, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI261862352, Length=447, Percent_Identity=43.8478747203579, Blast_Score=358, Evalue=8e-99,
Organism=Homo sapiens, GI261862350, Length=447, Percent_Identity=43.8478747203579, Blast_Score=358, Evalue=8e-99,
Organism=Homo sapiens, GI261862348, Length=447, Percent_Identity=43.8478747203579, Blast_Score=358, Evalue=8e-99,
Organism=Homo sapiens, GI261862346, Length=447, Percent_Identity=43.1767337807606, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI22547186, Length=402, Percent_Identity=46.0199004975124, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=44.8453608247423, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=59.5121951219512, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25144732, Length=409, Percent_Identity=46.9437652811736, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25144729, Length=409, Percent_Identity=46.9437652811736, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319739, Length=406, Percent_Identity=47.7832512315271, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323087, Length=410, Percent_Identity=44.8780487804878, Blast_Score=339, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24640005, Length=448, Percent_Identity=45.9821428571429, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI221329721, Length=448, Percent_Identity=45.9821428571429, Blast_Score=381, Evalue=1e-106,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45683; Mature: 45552

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKVTVMSYIEKIDPELFEAIKNEADRQEHKLNLIASENYASRAVMEAQGSIMTNKYAE
CCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEECHHCC
GYSGKRYYGGCDFVDVAENLAIARAKELFGAKYVNVQPHSGSGANMAVYFSVLQPGDTIL
CCCCCEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEECCCCEEE
SMDLSHGGHLSHGSPVSFSGKLYNIVPYGVSKETEALDYDELLKLAKECKPRMIVCGASA
EEECCCCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCC
YPRVIDFKRFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTTTTHKTLRGPRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCC
GMIISKTEELAIGVNKAVFPGIQGGPLMHIIAAKAVAFKEAMSEEFRQDQDQTVKNAKVL
CEEEECCHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CSCLKQKGFDIVSGGTDNHLMLVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFV
HHHHHHCCCEEEECCCCCEEEEEEECCCEECCCCHHHHHHHCCEEECCCCCCCCCCCCCC
TSGVRLGTPSCTTRGMKEKEMELVADYIEAAIKNSENDALLSEINIKVRDLCLKFPVYE
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHEEHHHHHCCCCC
>Mature Secondary Structure 
SYKVTVMSYIEKIDPELFEAIKNEADRQEHKLNLIASENYASRAVMEAQGSIMTNKYAE
CEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCEEECHHCC
GYSGKRYYGGCDFVDVAENLAIARAKELFGAKYVNVQPHSGSGANMAVYFSVLQPGDTIL
CCCCCEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEECCCCEEE
SMDLSHGGHLSHGSPVSFSGKLYNIVPYGVSKETEALDYDELLKLAKECKPRMIVCGASA
EEECCCCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCC
YPRVIDFKRFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTTTTHKTLRGPRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCC
GMIISKTEELAIGVNKAVFPGIQGGPLMHIIAAKAVAFKEAMSEEFRQDQDQTVKNAKVL
CEEEECCHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CSCLKQKGFDIVSGGTDNHLMLVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFV
HHHHHHCCCEEEECCCCCEEEEEEECCCEECCCCHHHHHHHCCEEECCCCCCCCCCCCCC
TSGVRLGTPSCTTRGMKEKEMELVADYIEAAIKNSENDALLSEINIKVRDLCLKFPVYE
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]