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The map label for this gene is folD [H]

Identifier: 21226543

GI number: 21226543

Start: 561382

End: 562245

Strand: Reverse

Name: folD [H]

Synonym: MM_0441

Alternate gene names: 21226543

Gene position: 562245-561382 (Counterclockwise)

Preceding gene: 21226544

Following gene: 21226542

Centisome position: 13.73

GC content: 47.69

Gene sequence:

>864_bases
TTGTTAGATGAAAGCTATGAGTCACGTATCATAGATGGAAAAGTCCTTGCACAGCAGGTCGAAGAAGAGGTAAGGTCAGG
AGTAGAAGACCTTGAAAGCAACAGGGGAATTACTCCCGGGCTTGCCACCATCCTTGTAGGGGATGACCCTGCATCAAAGA
TGTATGTCCGCCTGAAGCACAGAGCCTGTGAGCGTGTGGGCATAAGAGCAGAAGACTTCCTTCTTCCTGCAGATTCTACC
CAGGAAGAACTTCTTGCCCTGATCGATTCCCTTAACAATAACAAGGATGTGCACGGCATCCTGCTTCAGCTGCCTTTGCC
AGAGCATTTCTCTCCGCAGGAAGCAATGGAAGCCATATCTCCTGCAAAGGATGCAGACGGTTTTCATCCCTATAATATGG
GAAAACTCATGATAGGGGATGAAGGGCTTGTCCCATGTACTCCGCACGGGGTAATCAGGGCACTCGAAGAGTATAATGTG
CCTGTCAAGGGGAAAAATGCGGTTATTGTCGGACACAGCAATGTTGTGGGAAAACCAATGGCAGCAATGCTTCTTAATAG
AAATGCAAGCGTTTCTGTCTGCCACATCTTCACCGATGACCTGAAGAAATACACCCTTGGTGCCGATATCCTTGTTGTTG
CAGCAGGAGTCAAACACCTTATTAAAGCCGACATGGTTAAGGAAGGGGCTGTAATTTTCGATGTGGGAATCACCAAAGAA
GAGGACGGTGTATACGGGGACGTGGATTTCGAAAATGTAATCAAAAAAGCTTCCCTTATCACTCCCGTCCCTGGCGGTGT
AGGACCCCTGACAGTTGCCATGCTTATGAAGCACGTACTTATGTGTGCTGAGAAAAGCCTTTAA

Upstream 100 bases:

>100_bases
GGAAAACGATGCTCTTCTGTCGGAAATCAATATTAAGGTAAGGGATCTCTGTTTAAAGTTCCCTGTTTACGAATAATTGA
GGAGATAGGGGGTTTACCAT

Downstream 100 bases:

>100_bases
TTTTTTAATTTAAGGCTTTTCCTTTTATGATGAAAAGAGCCGGTTCAATTTTCCGGAAAAAACAAGTAAATTTGTTTTTT
GATTGCATCGAACTGGAGCC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLDESYESRIIDGKVLAQQVEEEVRSGVEDLESNRGITPGLATILVGDDPASKMYVRLKHRACERVGIRAEDFLLPADST
QEELLALIDSLNNNKDVHGILLQLPLPEHFSPQEAMEAISPAKDADGFHPYNMGKLMIGDEGLVPCTPHGVIRALEEYNV
PVKGKNAVIVGHSNVVGKPMAAMLLNRNASVSVCHIFTDDLKKYTLGADILVVAAGVKHLIKADMVKEGAVIFDVGITKE
EDGVYGDVDFENVIKKASLITPVPGGVGPLTVAMLMKHVLMCAEKSL

Sequences:

>Translated_287_residues
MLDESYESRIIDGKVLAQQVEEEVRSGVEDLESNRGITPGLATILVGDDPASKMYVRLKHRACERVGIRAEDFLLPADST
QEELLALIDSLNNNKDVHGILLQLPLPEHFSPQEAMEAISPAKDADGFHPYNMGKLMIGDEGLVPCTPHGVIRALEEYNV
PVKGKNAVIVGHSNVVGKPMAAMLLNRNASVSVCHIFTDDLKKYTLGADILVVAAGVKHLIKADMVKEGAVIFDVGITKE
EDGVYGDVDFENVIKKASLITPVPGGVGPLTVAMLMKHVLMCAEKSL
>Mature_287_residues
MLDESYESRIIDGKVLAQQVEEEVRSGVEDLESNRGITPGLATILVGDDPASKMYVRLKHRACERVGIRAEDFLLPADST
QEELLALIDSLNNNKDVHGILLQLPLPEHFSPQEAMEAISPAKDADGFHPYNMGKLMIGDEGLVPCTPHGVIRALEEYNV
PVKGKNAVIVGHSNVVGKPMAAMLLNRNASVSVCHIFTDDLKKYTLGADILVVAAGVKHLIKADMVKEGAVIFDVGITKE
EDGVYGDVDFENVIKKASLITPVPGGVGPLTVAMLMKHVLMCAEKSL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=46.6442953020134, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=46.1279461279461, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=41.0958904109589, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI36796743, Length=205, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=48.0286738351254, Blast_Score=260, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=37.890625, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=41.2371134020619, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=38.047138047138, Blast_Score=205, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6322933, Length=317, Percent_Identity=28.0757097791798, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=44.1471571906354, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=44.2567567567568, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=40.2061855670103, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=40.2061855670103, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=40.2061855670103, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=40.2061855670103, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31015; Mature: 31015

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDESYESRIIDGKVLAQQVEEEVRSGVEDLESNRGITPGLATILVGDDPASKMYVRLKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
RACERVGIRAEDFLLPADSTQEELLALIDSLNNNKDVHGILLQLPLPEHFSPQEAMEAIS
HHHHHHCCCHHHEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHC
PAKDADGFHPYNMGKLMIGDEGLVPCTPHGVIRALEEYNVPVKGKNAVIVGHSNVVGKPM
CCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHH
AAMLLNRNASVSVCHIFTDDLKKYTLGADILVVAAGVKHLIKADMVKEGAVIFDVGITKE
HHHHHCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
EDGVYGDVDFENVIKKASLITPVPGGVGPLTVAMLMKHVLMCAEKSL
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDESYESRIIDGKVLAQQVEEEVRSGVEDLESNRGITPGLATILVGDDPASKMYVRLKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
RACERVGIRAEDFLLPADSTQEELLALIDSLNNNKDVHGILLQLPLPEHFSPQEAMEAIS
HHHHHHCCCHHHEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHC
PAKDADGFHPYNMGKLMIGDEGLVPCTPHGVIRALEEYNVPVKGKNAVIVGHSNVVGKPM
CCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHH
AAMLLNRNASVSVCHIFTDDLKKYTLGADILVVAAGVKHLIKADMVKEGAVIFDVGITKE
HHHHHCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
EDGVYGDVDFENVIKKASLITPVPGGVGPLTVAMLMKHVLMCAEKSL
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]