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The map label for this gene is alaXM [H]

Identifier: 21226335

GI number: 21226335

Start: 312628

End: 313440

Strand: Reverse

Name: alaXM [H]

Synonym: MM_0233

Alternate gene names: 21226335

Gene position: 313440-312628 (Counterclockwise)

Preceding gene: 21226336

Following gene: 21226333

Centisome position: 7.65

GC content: 45.26

Gene sequence:

>813_bases
TTGAATGCCGCTTTTAAAGATGAGTCTATAAAAGTTCCTGACCGGAGAGGTTTACCAATGACAGAAGCGCTTTACTTCCT
TGACTGTTACCTGAAAGAATTCGAAGCAACCGTTGAAAAAGTGACTGAAGGCAAGTATATTGTCCTTGACCGCACTGCTT
TCTATCCCGAGAGCGGCGGGCAGCCCAGTGATACCGGTAAACTGGTCCGGGAAAGGGACGGGGCTGAATTTAAGGTTGTT
TATGCGGGCAAGTTCAATGGAGACATAAGCCACGAGATCTCTCCTGAAGGCGAAACCGGAGCAGAAGGTCTTAAGGTTGG
AGATAAAGTAAAAGGTATTATTGACTGGGACCGCCGTTACAGGCATATGCGCATGCATACTGCAACCCATGTGATTGCAA
ATGTGATCGAAAAAGAAGCAGGAGCCCAGATTACCGGAAACCAGCTGGGGCTTGACCAGAGCAGGGTTGATTTCAGCCTT
GAGGCTTTTGACAGGGAAAAGTTCGCAGAATACGAAAAAATCGCAAATGAAATAATAGCCGAAAACCATTCCGTAAATCT
CTACCTTGTCAGCCGCAAAGAAGCTGAAGAAAGATTATCAAGGCTTACCACGCTTGCCAAAGGTTTCTCTGAAGAGATAA
CTGAAGTCCGCCTTGTTGAAATCGAGGGCGTGACAATTGAAGCCTGTGGAGGGTCACACCTTAAAAATACAGGAGAGATT
AAAGGAATAAAGATTGAAAAACTCCAGAACAAAGGGAAAAGTAACAGGAGAATGTACTTTTCGCTCCTGGACCAAGCTTC
TGGACTGAAATAA

Upstream 100 bases:

>100_bases
GAGCATCCAGTTACCTGATAGTATATTTTTGATTCATGTGCAGGCATTATTTTGATTATAATTTGTACTGAGATGTGGCT
ATTTGAGATTTGCCGGCTTC

Downstream 100 bases:

>100_bases
CCAGGTGAGTTGAATTTCTATGAAATCTTTTTCCTTTAATTTTTAACAGCAGGCTATTGCAGGCTATTAACAGTGCTTAG
CTACAGACTAAATACAGACG

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; AlaXp; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNAAFKDESIKVPDRRGLPMTEALYFLDCYLKEFEATVEKVTEGKYIVLDRTAFYPESGGQPSDTGKLVRERDGAEFKVV
YAGKFNGDISHEISPEGETGAEGLKVGDKVKGIIDWDRRYRHMRMHTATHVIANVIEKEAGAQITGNQLGLDQSRVDFSL
EAFDREKFAEYEKIANEIIAENHSVNLYLVSRKEAEERLSRLTTLAKGFSEEITEVRLVEIEGVTIEACGGSHLKNTGEI
KGIKIEKLQNKGKSNRRMYFSLLDQASGLK

Sequences:

>Translated_270_residues
MNAAFKDESIKVPDRRGLPMTEALYFLDCYLKEFEATVEKVTEGKYIVLDRTAFYPESGGQPSDTGKLVRERDGAEFKVV
YAGKFNGDISHEISPEGETGAEGLKVGDKVKGIIDWDRRYRHMRMHTATHVIANVIEKEAGAQITGNQLGLDQSRVDFSL
EAFDREKFAEYEKIANEIIAENHSVNLYLVSRKEAEERLSRLTTLAKGFSEEITEVRLVEIEGVTIEACGGSHLKNTGEI
KGIKIEKLQNKGKSNRRMYFSLLDQASGLK
>Mature_270_residues
MNAAFKDESIKVPDRRGLPMTEALYFLDCYLKEFEATVEKVTEGKYIVLDRTAFYPESGGQPSDTGKLVRERDGAEFKVV
YAGKFNGDISHEISPEGETGAEGLKVGDKVKGIIDWDRRYRHMRMHTATHVIANVIEKEAGAQITGNQLGLDQSRVDFSL
EAFDREKFAEYEKIANEIIAENHSVNLYLVSRKEAEERLSRLTTLAKGFSEEITEVRLVEIEGVTIEACGGSHLKNTGEI
KGIKIEKLQNKGKSNRRMYFSLLDQASGLK

Specific function: Functions in trans to edit the amino acid moiety from mischarged Ser-tRNA(Ala). Recognition depends, at least in part, on the acceptor stem of tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=256, Percent_Identity=28.515625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI13376886, Length=256, Percent_Identity=28.515625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI109148542, Length=242, Percent_Identity=30.5785123966942, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1789048, Length=219, Percent_Identity=31.0502283105023, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17506981, Length=232, Percent_Identity=30.1724137931034, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324911, Length=241, Percent_Identity=32.7800829875519, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324288, Length=243, Percent_Identity=25.1028806584362, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582809, Length=245, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI45552267, Length=245, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI18543305, Length=231, Percent_Identity=29.4372294372294, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 30330; Mature: 30330

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAFKDESIKVPDRRGLPMTEALYFLDCYLKEFEATVEKVTEGKYIVLDRTAFYPESGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCC
QPSDTGKLVRERDGAEFKVVYAGKFNGDISHEISPEGETGAEGLKVGDKVKGIIDWDRRY
CCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECHHHHH
RHMRMHTATHVIANVIEKEAGAQITGNQLGLDQSRVDFSLEAFDREKFAEYEKIANEIIA
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHCEEHHHHCHHHHHHHHHHHHHHHH
ENHSVNLYLVSRKEAEERLSRLTTLAKGFSEEITEVRLVEIEGVTIEACGGSHLKNTGEI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEECCCCCCCCCCCC
KGIKIEKLQNKGKSNRRMYFSLLDQASGLK
CCEEHHHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNAAFKDESIKVPDRRGLPMTEALYFLDCYLKEFEATVEKVTEGKYIVLDRTAFYPESGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCC
QPSDTGKLVRERDGAEFKVVYAGKFNGDISHEISPEGETGAEGLKVGDKVKGIIDWDRRY
CCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECHHHHH
RHMRMHTATHVIANVIEKEAGAQITGNQLGLDQSRVDFSLEAFDREKFAEYEKIANEIIA
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHCEEHHHHCHHHHHHHHHHHHHHHH
ENHSVNLYLVSRKEAEERLSRLTTLAKGFSEEITEVRLVEIEGVTIEACGGSHLKNTGEI
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEECCCCCCCCCCCC
KGIKIEKLQNKGKSNRRMYFSLLDQASGLK
CCEEHHHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]