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The map label for this gene is purD [H]

Identifier: 21226246

GI number: 21226246

Start: 178642

End: 179949

Strand: Direct

Name: purD [H]

Synonym: MM_0144

Alternate gene names: 21226246

Gene position: 178642-179949 (Clockwise)

Preceding gene: 21226244

Following gene: 161485684

Centisome position: 4.36

GC content: 46.94

Gene sequence:

>1308_bases
ATGAAAATGAAAATTTTGCTTATCGGCGGCGGCGGAAGGGAGCACGCTATTGCCGAAGGAATTAAGAAAAGCAAGCATAA
CCCAATCCTTTATGCGTTAATGGCAAAGAAAAATCCGGGAATTGCCGCCCTCTGTGAGGACTTCCTGCTCGAAAAAGAAA
CAGAGGTTGAAAAAGTTGTCGAATATGCAAAAGCCAGGAAGATAGAAATGGCTTTTGTGGGGCCTGAAGCTCCCCTTGCA
GCAGGAGTTGCAGATGCCCTATGGGATGCCGGAATTCCCGTTGTAGGACCTAAAAAAGCCTGTGCAATTATTGAATTTGA
CAAGGCGTGGGCAAGAAATTTCATGAAGAAATACGGGATCGAAGGCTGCCCTGCCTATGAGGTTTTTACGGAAGAAGCTC
CTGCACACGCTTTCATTGAAAAGCTTGGTGACGTTGCAGTTAAGCCCTCGGGACTTACAGGCGGCAAAGGTGTAAAAGTC
ATGGGCGACCAGCTACCTGACATCAAAGCTGCCAGGGAATACACAAGCGAGCTTCTGGAAAAGGGACCTGTTGTTATTGA
GGAGCGTTTCATAGGAGAAGAATTTACCCTCCAGGCTTTTGTTGACGGAAAAAACCTTGCCTTTTTCCCTGCAGTGCAGG
ACCATAAGAGGGCTTATGAAGGGGACATCGGGCCAAATACTGGCGGCATGGGCTCTTACACGGATGCAGGAGAGATCCTT
CCTTTCATGGTCCCGGAAGATATCGAGAAGGCAAAAAAGATTATGCAGCATACCGTAACAGCCCTTCGTGAGGAAACCGG
AACGGGATACCAGGGGATTCTTTACGGGCAGTTTATCCTTACATCCAGAGGCCCAAAAGTCGTGGAGTTCAATGCCAGGT
TCGGAGATCCTGAAGCCATGAACGTTATTCCCCTTATAGAGACTGATTTTGTAGAAATAATGTCTGCCGTGACAAAAGGA
ACCCTTGGAGACCTGCCTGTGAAATTCAGCAGGAAAGCTACAGTCTGCAAATACGCAGTTCCTGCAGGCTACCCGGATAA
CCCGAAAAAAGACAGCGAAGTGATTGTAGGAGATATAGGGGAAGCTTCAGTTTACTATGCAAGTGTGTATGAAAAAGAAG
GGAAAATTTATACCACCACTTCCCGCGCCGTTGCTGTTGTCGGGTGTGCAGAAACAATAGAGGCTGCAGAGAAAGTCGCT
CAGAATGCTCTTGAAAATATTCAGGGTGAACTTTTCTTCAGGAGAGATATAGGCACAGCGAGTCTTATCCAGAAAAGAAT
AGACCATATGAAAGAACTTAGAGGCTGA

Upstream 100 bases:

>100_bases
TTGTGAGTTCCATAATCCATTACCTTTTTTTTCACGATATTTCCTCAAAGGCATTAAGAACAAATTTCAATTGAAAAATA
AATTTTTTTGAACAAGGAGA

Downstream 100 bases:

>100_bases
TTGTTAAAAACTATGCATAAGTGCCGCATCTAAATATGTGTAAATAAGTACTATGCAGTAATAATAAAGCCTGGAAACAA
AACAGAGCCTGGAAATAAAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKMKILLIGGGGREHAIAEGIKKSKHNPILYALMAKKNPGIAALCEDFLLEKETEVEKVVEYAKARKIEMAFVGPEAPLA
AGVADALWDAGIPVVGPKKACAIIEFDKAWARNFMKKYGIEGCPAYEVFTEEAPAHAFIEKLGDVAVKPSGLTGGKGVKV
MGDQLPDIKAAREYTSELLEKGPVVIEERFIGEEFTLQAFVDGKNLAFFPAVQDHKRAYEGDIGPNTGGMGSYTDAGEIL
PFMVPEDIEKAKKIMQHTVTALREETGTGYQGILYGQFILTSRGPKVVEFNARFGDPEAMNVIPLIETDFVEIMSAVTKG
TLGDLPVKFSRKATVCKYAVPAGYPDNPKKDSEVIVGDIGEASVYYASVYEKEGKIYTTTSRAVAVVGCAETIEAAEKVA
QNALENIQGELFFRRDIGTASLIQKRIDHMKELRG

Sequences:

>Translated_435_residues
MKMKILLIGGGGREHAIAEGIKKSKHNPILYALMAKKNPGIAALCEDFLLEKETEVEKVVEYAKARKIEMAFVGPEAPLA
AGVADALWDAGIPVVGPKKACAIIEFDKAWARNFMKKYGIEGCPAYEVFTEEAPAHAFIEKLGDVAVKPSGLTGGKGVKV
MGDQLPDIKAAREYTSELLEKGPVVIEERFIGEEFTLQAFVDGKNLAFFPAVQDHKRAYEGDIGPNTGGMGSYTDAGEIL
PFMVPEDIEKAKKIMQHTVTALREETGTGYQGILYGQFILTSRGPKVVEFNARFGDPEAMNVIPLIETDFVEIMSAVTKG
TLGDLPVKFSRKATVCKYAVPAGYPDNPKKDSEVIVGDIGEASVYYASVYEKEGKIYTTTSRAVAVVGCAETIEAAEKVA
QNALENIQGELFFRRDIGTASLIQKRIDHMKELRG
>Mature_435_residues
MKMKILLIGGGGREHAIAEGIKKSKHNPILYALMAKKNPGIAALCEDFLLEKETEVEKVVEYAKARKIEMAFVGPEAPLA
AGVADALWDAGIPVVGPKKACAIIEFDKAWARNFMKKYGIEGCPAYEVFTEEAPAHAFIEKLGDVAVKPSGLTGGKGVKV
MGDQLPDIKAAREYTSELLEKGPVVIEERFIGEEFTLQAFVDGKNLAFFPAVQDHKRAYEGDIGPNTGGMGSYTDAGEIL
PFMVPEDIEKAKKIMQHTVTALREETGTGYQGILYGQFILTSRGPKVVEFNARFGDPEAMNVIPLIETDFVEIMSAVTKG
TLGDLPVKFSRKATVCKYAVPAGYPDNPKKDSEVIVGDIGEASVYYASVYEKEGKIYTTTSRAVAVVGCAETIEAAEKVA
QNALENIQGELFFRRDIGTASLIQKRIDHMKELRG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=431, Percent_Identity=35.7308584686775, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI209869995, Length=431, Percent_Identity=35.7308584686775, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI209869993, Length=431, Percent_Identity=35.7308584686775, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI28416899, Length=431, Percent_Identity=35.7308584686775, Blast_Score=232, Evalue=6e-61,
Organism=Escherichia coli, GI1790438, Length=437, Percent_Identity=31.8077803203661, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=31.8604651162791, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=27.9642058165548, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24582400, Length=362, Percent_Identity=38.3977900552486, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI62471619, Length=362, Percent_Identity=38.3977900552486, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47325; Mature: 47325

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKILLIGGGGREHAIAEGIKKSKHNPILYALMAKKNPGIAALCEDFLLEKETEVEKVV
CEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHH
EYAKARKIEMAFVGPEAPLAAGVADALWDAGIPVVGPKKACAIIEFDKAWARNFMKKYGI
HHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCC
EGCPAYEVFTEEAPAHAFIEKLGDVAVKPSGLTGGKGVKVMGDQLPDIKAAREYTSELLE
CCCCCHHHHHCCCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
KGPVVIEERFIGEEFTLQAFVDGKNLAFFPAVQDHKRAYEGDIGPNTGGMGSYTDAGEIL
CCCEEEEHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PFMVPEDIEKAKKIMQHTVTALREETGTGYQGILYGQFILTSRGPKVVEFNARFGDPEAM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCC
NVIPLIETDFVEIMSAVTKGTLGDLPVKFSRKATVCKYAVPAGYPDNPKKDSEVIVGDIG
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCC
EASVYYASVYEKEGKIYTTTSRAVAVVGCAETIEAAEKVAQNALENIQGELFFRRDIGTA
CCCEEEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
SLIQKRIDHMKELRG
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMKILLIGGGGREHAIAEGIKKSKHNPILYALMAKKNPGIAALCEDFLLEKETEVEKVV
CEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHH
EYAKARKIEMAFVGPEAPLAAGVADALWDAGIPVVGPKKACAIIEFDKAWARNFMKKYGI
HHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCC
EGCPAYEVFTEEAPAHAFIEKLGDVAVKPSGLTGGKGVKVMGDQLPDIKAAREYTSELLE
CCCCCHHHHHCCCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
KGPVVIEERFIGEEFTLQAFVDGKNLAFFPAVQDHKRAYEGDIGPNTGGMGSYTDAGEIL
CCCEEEEHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PFMVPEDIEKAKKIMQHTVTALREETGTGYQGILYGQFILTSRGPKVVEFNARFGDPEAM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCC
NVIPLIETDFVEIMSAVTKGTLGDLPVKFSRKATVCKYAVPAGYPDNPKKDSEVIVGDIG
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCC
EASVYYASVYEKEGKIYTTTSRAVAVVGCAETIEAAEKVAQNALENIQGELFFRRDIGTA
CCCEEEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
SLIQKRIDHMKELRG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]